• 폴리머
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• 제27권5호
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• pp.397-404
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• 2003

조직공학적 지지체에서의 골수유래 간엽줄기세포의 점착과 성장에 있어서 세포 점착성 단백질과 폴리펩타이드와의 상호작용을 조사하였다. 세포 점착성 물질로 알려진 단백질이나 폴리펩타이드는 락타이드-글리콜라이드 공중합체인 PLGA 필름과 지지체에 흡착하여 코팅되었으며, 이에 골수유래 간엽줄기 세포의 점착과 성장 거동을 비교하였다. 이들 단백질과 폴리펩타이드는 콜라겐 IV형과 피브리노겐, 라미닌, 젤라틴, 피브로넥틴, 폴리(L-라이신)이 사용되었다. 이중 폴리(L-라이신)을 제외한 단백질과 폴리펩타이드는 PLGA 필름 표면에 거의 단층으로 덮어져 흡착되었으며, PLGA 필름과 지지체에서 골수유래 간엽줄기세포가 1일과 2일, 4일간 배양되었다. 세포의 점착과 성장 거동은 sulforhodamine B법으로 평가하였다. PLGA 필름과 지지체에 단백질이나 폴리펩타이드가 흡착되지 않은 표면보다는 흡착된 표면에서의 세포의 점착과 성장이 우수하였다.

• 폴리머
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• 제30권5호
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• pp.445-452
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• 2006

본 연구에서는 슈반세포와 다양한 세포 부착인자로 코팅된 고분자 표면과의 상호관계에 대해 연구하였다. 세포 접착인자로 알려진 피브로넥틴, 피브리노겐, 라미닌, 비트로넥틴, 폴리-D-라이신 및 폴리-L-라이신을 PLGA 필름에 코팅하고 물 접촉각 측정과 ESCA 분석을 실시해 표면특성을 평가하구 접착인자로 코팅하거나 하지 않은 PLGA 필름의 표면에 슈반세포를 배양한 후 세포 계수와 SEM 관찰을 통해 세포 부착과 성장을 알아보았다. 세포 계수 결과에서 세포 부착은 PLGA 표면의 단백질 흡착과 관련 있음을 확인할 수 있었으며, 세포의 성장은 배양액의 우태아혈청 함량의 영향을 받는 것으로 나타났다. 이러한 결과를 통해 슈반세포의 접착과 성장이 특정한 세포 접착인자에 의해 영향을 받음을 알 수 있었다. 본 실험의 결과를 통해 조직공학적 신경 재생에 응용하기 위한 신경유도관의 개발에서 세포의 부착과 성장을 향상시키기 위해서는 세포의 종류 및 배양조건에 따라 신경유도관이 적절한 표면환경을 제공해야 함이 필수적임을 알 수 있었다.

• 대한화장품학회지
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• 제25권4호
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• pp.57-63
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• 1999

Pericarpium castaneae extracts have variously potent activities, such as anti-oxidative activity and free radical scavenging activity. in vivo and in vitro studies both indicate that pericarpium castaneae extracts acts as a free radical scavenger($IC_{50}:7.6{\mu}g/ml$) stronger than gallic acid($IC_{50}:12.5{\mu}g/ml$) and ellagic acid($IC_{50}:15{\mu}g/ml$) which could prevent cutaneous UV damages and skin aging. The extracts showed a good effect as a anti-oxidant($IC_{50}:50{\mu}g/ml$). It was shown that the appearance of wrinkle in human skin was reduced by topical application of pericarpium castaneae extracts. And the treatment of human skin with the extracts increased the elasticity and moisture of the skin. We investigated the effect of the pericarpium castaneae extracts on production of extracellular matrix using cultured A431 fibroblast cells. The results indicated that the extracts had no detectable effect on collagen synthesis. But synthesis of cell adhesion protein was increased by the extracts. The results suggest that increase of cell adhesion protein synthesis by pericarpium castaneae extracts has closely related to reduction of wrinkle in skin.

• 대한화장품학회:학술대회논문집
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• 대한화장품학회 1999년도 IFSCC . ASCS 학술대회 발표 논문
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• pp.57-64
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• 1999

Pericarpium castaneae extracts have variously potent activities, such as anti-oxidative activity and free radical scavenging activity. in vivo and in vivo studies both indicate that pericarpium castaneae extracts acts as a flee radical scavenger ($IC_{50}$/: 7.6$\mu\textrm{g}$/ml) stronger than gallic acid($IC_{50}$/: 12.5$\mu\textrm{g}$/ml) and ellagic acid($IC_{50}$/: 15$\mu\textrm{g}$/ml) which could prevent cutaneous UV damages and skin aging. The extracts showed a good effect as a anti-oxidant ($IC_{50}$/: 50$\mu\textrm{g}$/ml). It was shown that the appearance of wrinkle in human skin was reduced by topical application of pericarpium castaneae extracts. And the treatment of human skin with the extracts increased the elasticity and moisture of the skin. We investigated the effect of tile pericarpium castaneae extracts on production of extracellular matrix using cultured A431 fibroblast cells. The results indicated that the extracts had no detectable effect on collagen synthesis, But synthesis of cell adhesion protein was increased by the extracts. The results suggest that increase of cell adhesion protein synthesis by pericarpium castaneae extracts has closely related to reduction of wrinkle in skin.

• Bulletin of the Korean Chemical Society
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• 제33권10호
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• pp.3378-3382
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• 2012

A superhydrophobic surface with a water contact angle > $150^{\circ}$ has attracted great interest from both fundamental and practical aspects. In this study, we demonstrated that hydrophobicity of a silica nanotube (SNT) array can be easily controlled by the SNT aspect ratio. In addition, the adhesive and anti-adhesive properties were controlled without modifying the hydrophobic surface. Various silica structures on a polydimethylsiloxane substrate were prepared using the desired alumina template. Bundle-arrayed and bowl-arrayed silica surfaces exhibited extraordinary superhydrophobicity due to the large frontal surface area and hierarchical micro/nanostructure. As the strategy used in this study is biocompatible and a wide range of hydrophobicities are capable of being controlled by the SNT aspect ratio, a hydrophobic surface composed of an SNT array could be an attractive candidate for bioapplications, such as cell and protein chips.

• 대한의용생체공학회:학술대회논문집
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• 대한의용생체공학회 1989년도 춘계학술대회
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• pp.1-3
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• 1989

We have treated polymer surfaces such as polyethylene, polystyrene and polyester by various physicochemical and biological surface modification methods to be suitable for cell adhesion. The physicochemical methods we used were $O_2$ plasma discharge, corona discharge, sulfuric acid and chloric acid treatments. For the biological treatments, blood proteins such as plasma protein, serum protein and fibronectin were adsorbed onto the polymer surfaces. Chinese Hamster Ovary (CHO) cells were cultured on the surface-modified polymers and the cell-compatibility of those surfaces were compared. The chloric acid and fibronectin treatments were found to be the best methods of rendering the polymer surfaces adhesive for CHO cells.

• 미생물학회지
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• 제27권3호
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• pp.259-264
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• 1989

The adherence of $^{3}H$-labeled oral streptococcal cells to protein-coated hydroxyapatite (HA) beads was studied by a standard adherence assay. The adherence equilibrium for S. mutans 10449 occured in about 2 hrs. The cell numbers adhering to SHA was 50% less than those on bare HA. Sailva from different subjects had varying effect on bacterial adherence. The use of saliva adsorbed with homologouis bacteria decreased S. mutans adherence by 38% ; this indicates the presence of salivary agglutinin in acquired pellicle formed on HA. Animal sera and BSA decreased S. sanguis adherence. BSA concentration as high as 10mg/ml caused up to 87% adherence inhibition. The desorption experiment of adhered bacteria confirmed the previous reports that the adhesive sites on HA beads for S. mutans were different from those for S. sanguis and that S. mutans could enhance the adherence of S. sanguis but not vice versa.

• Journal of Microbiology and Biotechnology
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• 제20권6호
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• pp.978-984
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• 2010

This paper examines the probiotic bacterium Lactobacillus rhamnosus GG, and how it reacts to the presence of mucin in its extracellular milieu. Parameters studied included cell clustering, adhesion to mucin, extracellular protein production, and formation of final metabolites. L. rhamnosus GG was found to grow efficiently in the presence of glucose, N-acetylglucosamine, or mucin (partially purified or purified) as sole carbon sources. However, it was unable to grow using other mucin constituents, such as fucose or glucuronic acid. Mucin induced noticeable changes in all the parameters studied when compared with growth using glucose, including in the formation of cell clusters, which were easily disorganized with trypsin. Mucin increased adhesion of the bacterium, and modulated the production of extracellular proteins. SDS-PAGE revealed that mucin was not degraded during L. rhamnosus GG growth, suggesting that this bacterium is able to partially use the glucidic moiety of glycoprotein. This study goes some way towards developing an understanding of the metabolic and physiological changes that L. rhamnosus GG undergoes within the human gastrointestinal tract.

• 생명과학회지
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• 제17권7호통권87호
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• pp.1023-1025
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• 2007

Ajuba is a number of proteins containing cytosolic LIM domain. Its function may provide a new pathway whereby cell-cell adhesive events are transmitted to the nucleus to regulate cell proliferation and differentiation decisions. Here, Ajuba gene expression was investigated its molecular properties associated with endoplasmic reticulum (ER) stresses (tunicamycin, DTT, A23187 and BFA) which induced remarkable ex-pression of Ajuba mRNA. The mRNA half life of Ajuba was also determined, its half life of Ajuba mRNA in FRTL-5 cells was approximately 2 hr after the initial translation. Although the obvious bioligical function of Ajuba is not clear, on the base of the results, Ajuba gene expression is deeply associated with ER stresses.

• Natural Product Sciences
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• 제19권1호
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• pp.54-60
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• 2013

Rhus verniciflua Stokes (Anacadiaceae) is a plant that is native to East Asian countries, such as Korea, China, and Japan, and it has been found to exert various biological activities including antioxidative, anti-aggregatory, anti-inflammatory, anti-mutagenic, and apoptotic effects. Sulfuretin is one of the major flavonoid component isolated from the heartwood of R. verniciflua. Reactive oxygen species (ROS), produced via dental adhesive bleaching agents and pulpal disease, can cause oxidative stress. In the present study, we isolated sulfuretin from R. verniciflua and demonstrated that sulfuretin possesses cytoprotective effects against hydrogen peroxide ($H_2O_2$)-induced dental cell death. $H_2O_2$ is a representative ROS and causes cell death through necrosis in human dental pulp (HDP) cells. $H_2O_2$-induced cytotoxicity and production of ROS were blocked in the presence of sulfuretin, and these effects were dose dependent. Sulfuretin also increased heme oxygenase-1 (HO-1) protein expression. In addition, to determine whether sulfuretin-induced HO-1 expression mediated this cytoprotective effect, HDP cells were cotreated with sulfuretin in the absence or presence of SnPP, an inhibitor of HO activity. Sulfuretin-dependent HO-1 expression was required for suppression of $H_2O_2$-induced HDP cell death and ROS generation. These results indicate that sulfuretin-dependent HO-1 expression was required for the inhibition of $H_2O_2$-induced cell death and ROS generation. In addition, sulfuretin may be used to prevent functional dental cell death and thus may be useful as a pulpal disease agent.