• Title/Summary/Keyword: cccDNA breakage assay

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Use of cccDNA Breakage Assay and Sulforhodamine B Assay for the Prescreening of Antitumor Agents from Microbial Sources (미생물 배양액으로부터 항암제의 예비선별을 위한 cccDNA Breakage 활성검정과 Assay Sulforhodamine B 활성검정의 이용)

  • Lee, Sang-Han;Lee, Dong-Sun;Kim, Jong-Guk;Hong, Soon-Duck
    • Journal of Life Science
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    • v.8 no.1
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    • pp.67-71
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    • 1998

  • In order to develop new antitumor agents from fermentation broths, we used cccDNA breakage assay abd sulforhodamine B assay for prescreening. As a result, it was shown that sample reach 3.3% when using cccDNA breakage assay. In sulforhodamine B assay, we obtained 4 acive fraction against A549 (a cell line of human lung carcinoma) and SK-OV-3 (a cell line of human adenocarcinoma). These results suggest that these assay would be a promising method for antitumor prescreening from microbial sources.

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