• Biomedical Science Letters
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• v.17 no.4
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• pp.291-295
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• 2011

PD98059 is the specific inhibitor of extracellular signaling-regulated kinase (ERK) kinase (MEK). ERK is involved in a mitogen-activated protein kinase (MAPK) cascade controlling cell growth and differentiation. Although the inhibition of ERK is known to induce cell death in various cell lines, this effect is still controversial and the role of PD98059 on the death of HeLa $S_3$ cells, a subclone of the cervical cancer cell line, is not well understood. The apoptosis of HeLa $S_3$ cells increased after the treatment of 50 ${\mu}M$ PD98059. The induction of apoptosis by PD98059 was occurred in a time- and a dose-dependent manners. The expression of Bcl-2 was reduced in accordance with decrease of ERK2 expression. Taken together, these results indicate that PD98059 has a cytotoxicity in HeLa $S_3$ cells and it may be used as a potential target for the treatment of cervical cancer.

• Journal of Korean Institute of Industrial Engineers
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• v.25 no.1
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• pp.111-124
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• 1999

This evapaper is toluate the forecasting performance of three neural network(NN) approaches against ARIMA model using the famous time series analysis competition data. The first NN approach is to analyze the second Makridakis (M2) Competition Data using Multilayer Perceptron (MLP) that has been the most popular NN model in time series analysis. Since it is recently known that MLP suffers from bias/variance dilemma, two approaches are suggested in this study. The second approach adopts Cascade Correlation Network (CCN) that was suggested by Fahlman & Lebiere as an alternative to MLP. In the third approach, a time series is separated into two series using Noise Filtering Network (NFN) that utilizes autoassociative memory function of neural network. The forecasts in the decomposition analysis are the sum of two prediction values obtained from modeling each decomposed series, respectively. Among the three NN approaches, Decomposition Analysis shows the best forecasting performance on the M2 Competition Data, and is expected to be a promising tool in analyzing socio-economic time series data because it reduces the effect of noise or outliers that is an impediment to modeling the time series generating process.

• BMB Reports
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• v.33 no.4
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• pp.349-352
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• 2000

Proteins that are involved in cellular signal cascade experience phosphorylation and dephosphorylation cycles in their tyrosine residue(s) during cell adhesion. In order to identify the protein(s), which tyrosine desidues are specifically phosphorylated when the cells attached to the substrate, we compared the tyrosine phosphorylation level of proteins between suspension and adhered culture condition in rat fibroblast 3Yl cells. We found that a cluster of 70 kDa protein was specifically phosphorylated when the cells adhered to the substrate, but did not effect the cells held in suspension. The phosphorylated protein is identified as paxillin, a focal adhesion protein in immunoprecipitation and immunobloting analysis. These results suggest that the tyrosine phosphorylation of paxillin may play a role in cell-substrate adhesion.

• Proceedings of the PSK Conference
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• 2003.04a
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• pp.165.2-166
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• 2003

Diesel exhaust is suspected to cause acute and chronic adverse effects on health. In recent. the effect of estrogenic endocrine disruptor in diesel particulate matter was little studied. Therefore, we examined the estrogenic activity of respirable diesel exhaust particulate matter derived from diesel engine motor. PM2.5 diesel exhaust of vehicle was collected using a high volume samples equipped with a cascade impactor. (omitted)

• 제어로봇시스템학회:학술대회논문집
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• 2005.06a
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• pp.1637-1640
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• 2005

This paper presents a technique for electromagnetic field analysis on surge response due to Mid-span back-flashovers effects in lightning protection system of 500 kV EHV single circuit transmission tower by the neural networks method. These analyses are based on modeling lightning return stroke as well as on coupling the electromagnetic fields of the stroke channel to the line. The ground conductivity influences both the electric field as well as the coupling mechanism and hence the magnitude and wave shape of the induced voltage. The technique can be used to analyzed the corona voltage effect, the effective of stroke to the span tower, the surge impedance of transmission lines. The maximum voltage from flashovers effects in the lines. The model is compatible with general electromagnetic transients programs such as the ATP-EMTP. The simulation results show that this study analyses for time-domain with those produced by a cascade multi-section model, the surge impedance of a full-sized tower hit directly by a lightning stroke is discussed.

• 유체기계공업학회:학술대회논문집
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• 2001.11a
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• pp.113-119
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• 2001

Interaction of flow through an Impeller and a Vaned Diffuser in Centrifugal Compressor was investigated using the 3-dimensional Wavier-Stokes solution method. To consider the interaction effect of impeller and vaned diffuser, Inlet boundary conditions are imposed with the results of the steady calculation of the impeller and rotates with time. The results have been compared to steady computation results and experiment. From this, it is discussed about the compatability of the method and the advantage and disadvantage of the steady calculation.

• BMB Reports
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• v.34 no.5
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• pp.478-483
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• 2001

We examined the effect of CsCl and spermine on the induction of ornithine decarboxylase (ODC), a key enzyme in polyamine synthesis form Glycine max axes. Transcription of the ODC gene was induced by 0.1 and 1 mM of CsCl, and the amount of putrescine was increased 3.5-fold by 1 mM CsCl treatment. Spermine also induced the expression of the ODC gene in a die dependent manner. However, CsCl provoked an increase in the active phosphorylated ERK (pERK), a central element of the mitogen-activated protein kinase (MAPK) cascade. Our data demonstrates an interaction between the ODC induction and the MAPK signaling pathway, and suggests that the latter may be involved in cell signaling in salt-stressed plants.

• 한국전산유체공학회:학술대회논문집
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• 2008.03b
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• pp.648-651
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• 2008

Numerical calculations are performed to simulate the film cooling effect of turbine blade tip with squealer rim. Because of high temperature of inside rim, squealer rim is damaged easily. Therefore many various cooling systems were used. The calculations are based on 100,000 Reynolds number in linear cascade model. A blade has 2% tip clearance and 8.4% rim height. The axial chord length and turning angle is 237mm, 126$^{\circ}$. Numerical calculations are performed without and with film cooling. In a film cooling in the cavity, hot spots of cavity were cooled effectively. However hot spots of suction side rim still remains. The CFD results show that the circulation flow in cavity of squealer tip affects the temperature rise of squealer rim. To maintain the blade integrity and avoid the excessive hot spot of blade, rearrangement of cooling hole is needed.

• Microbiology and Biotechnology Letters
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• v.28 no.5
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• pp.285-290
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• 2000

The effects of dissolved oxy-gen(DO) concentration and DO control modes on cell growth in a 4L computer-controlled bioreactor system were investigated using Phellinus linteus WI-001, a producer of protein-polysaccharides having potent anticancer activi-ties. When DO was controlled at about 20%, maximum cell concentration and specific growth rate of the strain were observed to increase 36% and 64%, respectively, as compared to the experiment performed without DO control. By adopting cascade automatic control of DO ar 20% in a mixed automatic control mode using computer-con-trolled program, 19.5g/L of maximum cell concentration was obtained. These results showed that the mixed auto-matic control mode was the effective method for enhancing cell growth of the shear sensitive Phellinus linteus.

• The Transactions of the Korean Institute of Electrical Engineers B
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• v.54 no.11
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• pp.547-552
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• 2005

This paper describes a pseudo-random carrier PW technique for the power converters. The proposed method generates a new pseudo-random carrier by randomly composing a carrier with fixed frequency and a carrier with opposition phase. To confirm the validity of the proposed method, a single-phase multi-level inverter was implemented and tested. The experimental results show that the output voltage and current harmonics spectra of an inverter have broadening effect of harmonics, as only simple composition of fixed frequency carries.