• Plant Resources
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• v.1 no.1
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• pp.33-37
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• 1998

Carotenoid compounds in embryos of wild-type(WT) and viviparous mutants of maize(Zea mays L.) were analyzed using high performance liquid ehromatography (HPLC) with a photodiode array detector. Zeaxanthin accumulates in WT embryos as the major carotenoid. Phytoene accumulates in vp2 and vp5. Phytofluene in w3 and ${\xi}$-carotene in the vp9 mutant embryos. This indicates that the vp2 and vp5 mutants impair phytoene desaturase from 15-cis-phytoene to 15-cis-phytofluene. The w3 mutant has neither an isomerase from 15-cis-phytofluene to all-trans-phytofuene nor phytofluene desaturase from phytofluene to ${\xi}$-carotene. The vp9 mutant does not have the ${\xi}$-carotene desaturase from ${\xi}$-carotene to lycopene. Our analysis shows that the terminal carotenoid. ${\gamma}$-carotene(${\beta},{\Psi}$-carotene), accumulates in the vp7 mutant embryos. The ${\varepsilon}$-carotene(${\varepsilon},{\varepsilon}$-carotene), a product of ${\delta}$-carotene(${\varepsilon},{\Psi}$-carotene) in some plants, however, has not been found in maize embryos. The vp7 mutant impairs a cyclization step from ${\gamma}$-carotene to both ${\beta}$-carotene and ${\alpha}$-carotene. We suggest that monocyclic ${\gamma}$-carotene is the sole precursor of both bicyclic ${\beta}$-carotene(${\beta},{\beta}$-carotene) and ${\alpha}$-carotene(${\beta},{\varepsilon}$-carotene) in maize.

• Journal of Pharmaceutical Investigation
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• v.29 no.4
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• pp.323-327
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• 1999

To protect ${\beta}-carotene$ at the stomach and to release rapidly at the intestine we prepared alginate beads containing ${\beta}-carotene$. ${\beta}-carotene$ and alginate solution was homogenized and prepared o/w emulsion was prepared. It was poured into $Ca^{2+}$ solution through syringe needle. The gel was formed spontaneously and alginate beads containing ${\beta}-carotene$ were prepared. ${\beta}-Carotene$ was incorporated into the beads more than 95%. The release rate of ${\beta}-carotene$ was dependent on the concentration of $Ca^{2+}$, ${\beta}-carotene$ and surfactants. However, the concentration of alginate did not affect the release rate of ${\beta}-carotene$. The high concentration of $Ca^{2+}$ slowed down the release rate of ${\beta}-carotene$. The addition of surfactants in the ${\beta}-carotene$beads increased the release rate of ${\beta}-carotene$ in the order of Tween 80 > Cremophor > Span 20. The contents of ${\beta}-carotene$ and diameter of ${\beta}-carotene$ beads did not change significantly at $50^{\circ}C$ for 20 days.

• Journal of the Korean Society of Food Science and Nutrition
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• v.23 no.5
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• pp.743-749
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• 1994

This study was designed to investigate the effect of dietary ${\beta}-carotene$ level on the lipid metabolism and lipid peroxide metabolizing enzyme activities in rats. Male Sprague -Dawley rats were fed on diets containing five levels of ${\beta}-carotene$ (0, 10, 120, 1200, 12000mg/kg diet ; BC 0, BC 1, BC 2, BC 3, BC 4 group). The rats were sacrificed after 7 weeks of the feeding periods. Lipid peroxide value of mitochondrial fraction of rat liver was elevated in ${\beta}-carotene$ restriction group when compared to $\beta$ -carotene groups. Superxide dismutase activity increased significantly by ${\beta}-carotene$ supplementation. Both catalase and glutathione peroxidase activities were reduced with increasing ${\beta}-carotene$ supplementation, except only ${\beta}-carotene$ restriction group. In liver, the contents of total lipid and cholestero decreased by ${\beta}-carotene$ supplementation but triglyceride content was not different among treatment groups. HDL-and total cholesterol ratio in plasma of 12, 000 ${\beta}-carotene$ group decreased, and was similar to that of ${\beta}-carotene$ restriction group.

• Journal of the Korean Society of Food Science and Nutrition
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• v.20 no.1
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• pp.5-12
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• 1991

the Chinese cabbage kimchi was fermented with the various submaterials such as hot pep-per garlic ginger leek green onion fermented anchovy juice and sugar according to the average contents of each submaterial described in the 39 kinds of references. And then the effects of each submaterial and lactic acid bacteria such as L. brevis. Leu. mesenteroides. P cerevisiae and L. plantarum on the content of carotenes were investigated, The major carotene in kimchi was $\beta$-carotene. And also $\delta$-carotene and $\alpha$-carotene were detected. Contents of $\beta$-carotene and total carotene were high in the kimchi containing leek red pepper powder green onion and fermented anchovy juice as a submaterial. But the kimchi containing or omitting the other submaterials were litter affected to the contents of carotene. Contents of $\beta$-carotene and total carotene were high in kimchi fermented with Leu. msenteroides L. brevis and P. cerevi-siae as a starter but was low with L plasntarum.

• Journal of the Korean Society of Food Science and Nutrition
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• v.40 no.8
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• pp.1092-1098
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• 2011

The present study was conducted to investigate the effect of ${\beta}$-carotene on the antioxidant system of rats with diabetes. Forty Sprague Dawley rats were fed the AIN-76 control diet or the same diet supplemented with ${\beta}$-carotene (7.2 mg/kg diet) for 3 weeks, then diabetes was induced in half the rats by administering streptozotocin (45 mg/kg BW) into the femoral muscle. Diabetic and normal rats were fed the experimental diets for 2 more weeks. To investigate the effect of dietary ${\beta}$-carotene on diabetes, the activities of antioxidative enzymes and glutathione concentration were determined in normal and streptozotocin-induced diabetic rats. The plasma glucose levels in diabetic rats were not influenced by the dietary supplementation of ${\beta}$-carotene. Hepatic activities of catalase and superoxide dismutase in diabetic rats were significantly lower than those of control rats but ${\beta}$-carotene tended to induce these activities. Glutathione-S-transferase activity was not significantly different between experimental groups. Glucose-6-phosphatase activity was induced in diabetic rats, but dietary supplementation of ${\beta}$-carotene reduced this activity. The hepatic concentration of reduced glutathione in diabetic rats was lower than that of control rats, but dietary supplementation with ${\beta}$-carotene restored the content to some extent. These data suggest that diabetic rats are exposed to increased oxidative stress and that dietary supplementation with ${\beta}$-carotene may reduce its detrimental effects.

• Journal of the Korean Society of Food Science and Nutrition
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• v.24 no.4
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• pp.530-536
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• 1995

A study was performed to assess dietary intake and serum levels of $\beta$-carotene and the influence of preference for hot taste on $\beta$-carotene intake in Korean female college students. Daily intake of $\beta$-carotene was estimated with a questionnaire composed of food item of high $\beta$-carotene contents and the average daily intake level was 4089$\pm$2400$\mu\textrm{g}$. The major sources of $\beta$-carotene included carrot, pumpkin, spinach, tomatoes and red pepper powder. 14$\pm$10% of total dietary $\beta$-carotene intake was from foods containing red pepper powder. The average amount of red pepper powder added to bean sprout soupr were 0.32$\pm$0.34g. Subjects preferring hot taste added significantly larger amount of red pepper powder(p<0.05) than those not preferring hot taste. Subjects of the lowest quartile of $\beta$-carotene intake level showed the lowest precentage of subjects preferring hot taste. The average serum $\beta$-carotene concentration was 36.5$\mu\textrm{g}$/dl and large variation in the amount among the subject has been observed. The level of dietary $\beta$-carotene intake and serum $\beta$-carotene concentration of Korean female college students were not significantly correlated but both were higher than levels in reports from western countries. These results indicate that $\beta$-carotene intake levels of the subjects are adequate and the preference for hot taste affects $\beta$-carotene intake significantly.

• Journal of the East Asian Society of Dietary Life
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• v.3 no.2
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• pp.51-61
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• 1993

This study was designed to investigate the effect of dietary level of $\beta$-carotene on level of antioxidant nutrients of rat tissues. Male Sprague Dawley rats at the age of 30days were fed on diets containing different levels of $\beta$-carotene(0, 10, 120, 1200, 12000mg per kg diet). Body weight gain of rats fed with 12000mg $\beta$-carotene diet was significantly decreased, but liver and heart weights were not significantly different among groups, The content of total glutathione tended to decrease significantly in 12000mg $\beta$-carotene diet group when compared to $\beta$-carotene restriction group(BC O). However, total vitamin C content of liver showed the tendency to increase by $\beta$ -carotene supply up to 1200mg. But this tendency was not found in plasma, The content of zinc in liver and plasma was significantly decreased by $\beta$-carotene restriction. Alkaline phosphatase activity was significantly higher in 12000mg diet group. In case of $\beta$-carotene restriction group, fibroblasts were proliferated in portal endothelium, and the vacuolar size was enlarged more than the nuclear, In 12000mg diet group, hepatic vacuoles were extended, but their size was regular and the lysis of hepatocytes was observed. Also, fibroblasts were proliferated in portal endothelium and the regular vacuolar size was extended.

• Journal of Nutrition and Health
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• v.36 no.7
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• pp.675-683
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• 2003

This study investigated the effect of dietary $\beta$-carotene supplementation on lipid peroxidation and anti oxidative enzyme activity as indices of oxidative stress in diabetic rats. Fifty Sprague-Dawley male rats aging 7 weeks were used as experimental animals, which were divided into the non-diabetic control group and the diabetic group. The diabetic group received an intraperitoneal injection with streptozotocin to induce diabetes. Then the diabetic rats were divided into four dietary groups which contained different amounts of $\beta$-carotene; 0%, 0.002%, 0.02%, or 0.2% of the diet. The diabetic rats were fed the experimental diets and the non-diabetic rats were fed the basal diet without $\beta$-carotene supplementation for 2 weeks and then sacrificed. The diabetic group had a significantly higher blood glucose level than the non-diabetic group. However, blood glucose level were not significantly changed by the level of dietary $\beta$-carotene supplementation. Compared to the non-diabetic control group, the diabetic control group indicated a significant increase of plasma thiobarbituric acid reactive substance (TBARS). Liver TBARS level also tended to be higher in diabetic control group, although it was not significant. The $\beta$-carotene supplementation did not reduce plasma TBARS level. However, Liver TBARS level was significantly decreased when 0.02% or more $\beta$-carotene was supplemented in the diet. The liver lipofuscin level in the diabetic control group was higher than in the non-diabetic control group, but the effect of $\beta$-carotene supplementation did not show any differences. Superoxide dismutase activity was significantly lower in the diabetic group, but it was increased in groups receiving 0.02% or more $\beta$-carotene. Compared to the non-diabetic control group, lower activities of catalase and glutathione peroxidase were observed in the diabetic control group, although it was not significant. Catalase and glutathione peroxidase activities tended to increase as the levels of $\beta$-carotene supplementation increased, although it was not statistically significant. Therefore, it seems that dietary $\beta$-carotene supplementation might reduce diabetic complications by partly decreasing the lipid peroxidation and increasing the activity of antioxidative enzyme in diabetes.

• Korean Journal of Food Science and Technology
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• v.54 no.1
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• pp.66-74
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• 2022

To improve the solubility of β-carotene, three types of β-carotene-loaded nanocapsules were prepared using chitosan (CS) and two cross-linkers, sodium tripolyphosphate (TPP) and hyaluronic acid (HA), alone or in combination (CS-TPP, CS-TPP-HA, and CS-HA). The entrapment efficiency of all nanocapsules significantly increased with an increase in TPP and HA, with the efficiency ranging from 95% to 99%. The solubility of β-carotene was significantly improved by CS nanoencapsulation before and after lyophilization and during storage. CS/HA nanoencapsulation significantly improved (by 11-fold) the water solubility of β-carotene. In particular, CS/HA nanoencapsulation was the most effective in terms of not only the solubility of β-carotene, but also the redispersibility ratio. Therefore, CS/HA encapsulation could be useful for improving the solubility of poorly soluble active ingredients, such as β-carotene.

• Food Industry And Nutrition
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• v.7 no.3
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• pp.44-49
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• 2002

본 연구는 retinyl acetate와 $\beta$-carotene보충식이가 에탄올에 의한 산화적 손상에 미치는 영향을 조사하고자 흰쥐에게 비타민 A와 $\beta$-carotene을 보충한 식이를 5주간 급여한 후 에탄올을 급성으로 투여하여 혈청과 간 조직에서 생성되는 지질과 지질과산화물 함량 그리고 glucose-6-phosphatase 활성도를 측정하였다. 본 실험에서 얻은 결과를 요약하면 체중 증가량은 전 실험군에서 유의적인 차이없이 실험 기간 동안 증가되었으며 사료섭취량과 식이효율을 그리고 간 중량 역시 각 군 모두 유의적인 차이를 나타내지 않았다. 혈청 중성지질 함량은 대조군과 retinyl acetate 보충군에서 높은 함량을 보였으나 통계적인 유의성은 없었다. 총 콜레스테를 함량은 대조군과 $\beta$-carotene 보충군은 비슷한 수준을 나타낸 반면 retinyl acetate 보충군에서는 유의적으로 감소되었다. HDL-콜레스테롤 함량은 대조군에서 가장 높았으며 LDL-콜레스테롤 함량은 유의적이지는 않았으나 retinyl acetate 보충군에서 가장 낮은 함량을 보였다. 동맥경화지수는 전 실험군에서 유의적인 차이는 없었으나 retinyl acetate 보충군에서 가장 낮았다. 간 마이크로솜 내에서 지질과산화물 함량은 재도군에 비하여 $\beta$-carotene 보충군에서 유의적으로 감소되었다. Retinyl acetate와 $\beta$-carotene 보충군 사이에서는 유의적인 차이가 없었으나 급성적인 에탄올 투여시에 $\beta$-carotene의 보충 급여가 지질과산화반응에 방어효과가 있는 것으로 나타났다. 간 조직중의 glucose-6-phosphatase 활성도는 비타민을 정상적으로 공급시킨 대조군이 retinyl acetate나 B-carotene 보충군에 비하여 유의적으로 높았으며 retinyl acetat와 B-carotene 보충군 사이에는 유의적인 차이가 없었다.