• KOREAN JOURNAL OF CROP SCIENCE
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• v.41 no.2
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• pp.230-235
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• 1996

This study was conducted to investigate the effects of planting densities on sex change and fiber yield in hemp plants, local variety 'Gangweonjong' was planted with various planting densities (40 $\times$ 5, 40 $\times$ 10, 40 $\times$ 20, 60 $\times$ 5, 60 $\times$ 10, 60 $\times$ 20cm), and harvesting dates (June 24, July 9 and 24). The stem length was the longest in the space of 60x20cm(8, 333 plants per l0a). The diameters of stem was the thickest in the planting space of 40 $\times$ 20cm(12, 500 plants per l0a) The ratio of peeled fiber yield was the highest in the planting space of 40$\times$5cm(50, 000 plants per l0a). In general, the condition under denser planting space (40 $\times$ 5cm ; 50, 000 plants per l0a) led to an increase in the number of female plants.

• Journal of Conservation Science
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• v.35 no.5
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• pp.385-391
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• 2019

In order to examine the material characteristics of a black and white photograph of the Borobudur temple, in Indonesia, several analyses were conducted. A sample was taken from a black and white photograph; microscopy, Fourier transform-infrared spectroscopy, scanning electron microscopy/energy dispersive spectroscopy, X-ray diffraction, and X-ray fluorescence analyses showed that the black and white photograph was composed of four layers including a paper substrate, baryta, binder and surface layers. It was confirmed that the substrate was paper made of cellulose fiber from Cannabis sativa, the baryta layer was made of barium sulfate(BaSO₄) in powder form, the binder was an emulsion containing silver halide, and the surface protective layer was made of gelatin. Since photographs have different characteristics and require different preservation environments depending on the material of construction, it is necessary to study various black and white photographic material characteristics corresponding to each time period.

• The Journal of Korean Medicine
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• v.32 no.3
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• pp.35-43
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• 2011

Objectives: This study was performed to screen target-specific inhibitors of ${\beta}$-catenin/TCF signaling whose functional activation plays an important role in early events in carcinogenesis. Methods: To investigate the activation or suppression of ${\beta}$-catenin/TCF transcription, we established a transiently transfected cell line with a constitutively active ${\beta}$-catenin mutant gene whose product is not degraded. This cell line was also co-transfected with luciferase reporter gene constructs containing either an optimized (TOPflash) or mutant (FOPflash) TCF-binding element. We investigated cytotoxic effects using a 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium salt (MTS) assay. To find effective inhibitors of ${\beta}$-catenin/TCF signaling from medicinal herbs, the crude extracts of 99 types of medicinal herbs were screened using a luciferase assay system in HEK-293 and SH-SY5y cells. Results: At a concentration of $50{\mu}g$/ml, extracts of Angelica koreanae radix, Cannabis sativa semen, Ephedrae intermedia Schrenk radix, and Vitis rotundifolia fruit showed the following inhibitory effects on ${\beta}$-catenin/TCF signaling: $40{\pm}5.6%$, $23{\pm}6.1%$, $8{\pm}5.1%$, and $22{\pm}9.8%$ in ${\beta}$-catenin-activated HEK-293 cells and $9{\pm}4.7%$, $39{\pm}8.1%$, $39{\pm}6.4%$, and $42{\pm}10.1%$ in ${\beta}$-catenin-activated SH-SY5y cells, respectively. Crude extracts of E. radix were isolated by silica gel column chromatography, and two non-polar fractions of these extracts showed inhibitory effects on ${\beta}$-catenin/TCF signaling. Conclusions: In this study, we established a transiently transfected cell line as a screening system and found that various medicinal herb extracts had inhibitory effects on ${\beta}$signaling.

• Food Science of Animal Resources
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• v.34 no.2
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• pp.141-150
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• 2014

This study was conducted to determine the effects of hempseed (H) on performance, carcass traits, and antioxidant activity in Japanese quail (Coturnix coturnix japonica). A total of 192 quail with seven-days old were divided into four experimental groups with four replicates. The treatments were; i) Control diet (C, no hempseed); ii) 5% hempseed in diet (H5); iii) 10% hempseed in diet (H10); and iv) 20% hempseed in diet (H20). The body weight (BW) and feed intake (FI) of quail was determined at 7, 21 and 42 d of age. At 42 d of age four quail were slaughtered and the carcass and internal organ traits were determined. Malondialdehyde (MDA), catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), nitric oxide (NO) and total protein were determined in the blood serum end of the experiment. The BW of the groups were not significant at 7 and 21 d, however in the 20% hempseed group BW decreased at 42 d (p<0.05). The FI and feed conversion ratio were not significant among the treatment groups. The carcass, liver, intestine and heart weight and their percentage to carcass were significantly differ in treatment groups (p<0.05). The serum MDA and NO decreased in hempseed addition (p <0.001). The serum SOD, CAT and GSH-Px were increased by hempseed supplementation (p<0.001). In conclusion, hempseed supplementation to quail diets may not improve quail performance traits but increase antioxidant activity in blood.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2001.11b
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• pp.35-42
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• 2001

In order to obtain basic information for pure line breeding of hemp which is effective on the pure line preservation and propagation, three varieties were investigative from 1995 to 1996 at Mokpo Experiment Station, Honam Agriculture Experiment Station, and cutting parts, cutting times, cutting length and storage duration after cutting of plant were observed. Higher rooting ratio were observed in cutting of shoot and later branch, in July 5, optimum harvesting time and 5 days after harvesting time, in shorter storage duration,0 and 2 days. Also there were the highest rooting ratio increased by from 12% to 16% in shorter cutting of 10cm than 15cm long cutting in shoot, lateral branch and stem cuttings. Hemp cv. Korea showed higher rooting ratio than the other varieties, Japan and Turkey. Therefore, it was concluded that hemp, a dioecious plant, could be effectively propagated for the pure lines by cutting method, especially in cv. Korea.

• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.45 no.6
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• pp.30-35
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• 2013

To conserve wood resources for papermaking, chemical compositions of the hemp (Cannabis sativa L.) bast fiber cultivated in Korea such as holocellulose, ${\alpha}$-cellulose, lignin, alcohol-benzene extractives, hot and cold water extractives, and ash contents were investigated to manufacture the specialty packaging paper effectively. Significantly very low klason lignin content of 3.3% was accomplished by removing of the outer shell of bark. Laboratory soda pulping method which is very useful for the nonwood fiber was adapted, and it was found that there was no significant difference in both kappa number and H-factor between 25% and 30% NaOH charge. Hemp pulp cooked with the laboratory digester in 25% NaOH at $170^{\circ}C$ were mixed together with the wood pulp(NBKP:LBKP=1:1) in order to find the optimum mixture ratio which exhibited acceptable paper strength properties such as tensile index, burst index, and tear strength. When 10% of hemp soda pulps was mixed with 90% of wood pulps comprised of SwBKP and HwBKP (1:1), all physical strength increased significantly. The physical strength decreased as the amount of hemp pulp increased because the cell wall of bast fiber is very thick which causes low conformability and low fiber-fiber bonding. These results showed that paper made of hemp-wood pulp can be used for the specialty packaging paper which requires both the characteristic surface properties and the high physical strength of hemp fiber.

• Journal of the Korean Applied Science and Technology
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• v.34 no.3
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• pp.666-672
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• 2017

Hemp [Cannabis sativa L.] has been cultivated as a fiber crop for long history, but it was a good oil crop because its seed contain plenty of lipid which is high ratio of unsaturated fatty acid. Hemp seed oil was extracted with a extruding method in many countries including Europe. The color of oil extracted with extruding method is dark green which could be difficult to attract consumer's interest in Korea because of insufficient information about hemp seed oil. This study was conducted to know correct information about hemp seed oils which were extracted with pressuring and extruding methods. In extruding method, seeds were crushed during the extracting process and discharged oil cake in shape of thin ribbon, but maintained seed shape although the seed were slightly flatted in pressuring method. Oil yield were higher in the extruding method in comparison with pressuring method. The oil have lower degree of lightness but higher degree of greenness and yellowness in the extruding method in comparison with pressuring method because of higher content of chlorophyll A, B and carotenoid. In fatty acid composition, the ratio of palmitic acid, stearic acid, oleic acid and ${\gamma}$-linolenic acid were higher but linoleic acid and ${\alpha}$-linolenic acid were lower in the extruding method in comparison with pressuring method. The content of total tocopherol and ${\gamma}$-tocopherol were lower in the extruding method in comparison with pressuring method.

• Korean Journal of Environment and Ecology
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• v.23 no.1
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• pp.90-98
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• 2009

Every spring, Korea is always plagued by sandy dust from the western region of China and Mongolia. Yellow sand is causing an environmental problem to Japan and far into the American continent, let alone Korea. At present, the western region of China is going under desertification at a great speed due to climatic change and humans' damaging activities. To cope with this, each country including China is considering ecological restoration of deserts through planting. Accordingly, this research conducted a vegetation survey on Baicheng district which is a representative dry land of western China to obtain a basic data for ecological restoration of a desert. The survey revealed that Setaria viridis which invaded an arid land made a succession into Setaria viridis-Cannabis sativa var. fruderalis community together with Artemisia mongolica-Setaria viridis community due to the increase in salt concentration and alkalization subsequent to dryness. It was also found out that there finally formed Artemisia mongolica community on a flat intense in harsh wind and dryness with the continuous worsening of environmental conditions. There appeared a different type of vegetation on hilly districts where sporadic shade could come into being because the air humidity could be available relatively there. Frequently, typically appearing at the whole survey area, the Tributlus terrestris community was found to make succession into Tribulus terrestris-Cleisrogenes squarrosa community due to the aggravation of soil environment. In addition, with the worsening of the environment at hilly districts, there formed Clesirogenes squarrosa community resistant to dryness, salinity in soil and strong alkalinity. Further, there appeared higher plant life totalling to 62 taxa comprising 58 species and 4 varieties with 27 families and 49 genuses at the whole survey area. Among these, Compositae plants excellent in resistance to environment was surveyed the most, accounting for 27%.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.51 no.5
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• pp.483-490
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• 2006

In order to breed hemp variety with nondrug type adapted to Korea, a total of 44 accessions were introduced from Center for plant breeding and reproduction research (CPRO) In Netherland and evaluated for plant growth characteristics and contents of cannabinoids such as tetrahydrocannbinol (THC), cannabidiol (CBD), Cannabinol (CBN) in Korean climatical and geographical condition. 1. In Korean geographical position which latitude is $34^{\circ}N$, days to flowering and stem length were shorter than in Netherlands which latitude is $52^{\circ}N$. But THC content was not shown significant difference between two areas. 2. Corrected accessions of hemp were classified into 3 variety groups by cluster analysis and 4 accessions including Korean local variety were not classified into any variety group. 3. Most hemp accessions have low THC contents but were not suitable for fiber production in Korea due to short days to flowering and stem length. 4. Among the introduced accessions, IH3 was suitable for breeding material of fiber hemp with non-drug type because of low THC content and similar days to flowering with Korean local variety.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.51 no.3
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• pp.187-190
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• 2006

In order to produce hemp with low cannabinoids and appropriate fiber yield, seeds of the IH3 (non drug type germplasm) and local variety (drug type cultivar) were mixed to 1:1, 2:1, 3:1 on base of seed weight ratio and cultivated for seed production. In the seed yield trial, the weight of 1000 grains were around 21 g, which was increased in proportion to the ratio of IH3 seeds but seed yield were 100 kg/10a, which were not significantly different by the seeds-mixing ratio. In fiber yield trials with seeds from seed yield trial, fiber yield ranged $193{\sim}198kg/10a$ which was not significantly different by the seeds mixing ratio but the content of THC(${\Delta}$-9-tetrahydrocannabinol) was lowered in proportion to the ratio of IH3 seeds. Variation of THC and CBD (Cannabidiol) level in the 150 plant cultivated with various seed mixing ratio reflected that the percentage of drug and intermediate type were decreased but non drug type were increased in proportion to the ratio of IH3 seeds.