• 제목/요약/키워드: cage experiment

검색결과 178건 처리시간 0.022초

Effects of $1{\alpha}$-Hydroxycholecalciferol and Phytase on Growth Performance, Tibia Parameter and Meat Quality of 1- to 21-d-old Broilers

  • Han, J.C.;Yang, X.D.;Zhang, L.M.;Li, W.L.;Zhang, T.;Zhang, Z.Y.;Yao, J.H.
    • Asian-Australasian Journal of Animal Sciences
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    • 제22권6호
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    • pp.857-864
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    • 2009
  • This experiment was conducted to investigate the effects of interaction between $1{\alpha}$-hydroxycholecalciferol ($1{\alpha}$-OH $D_3$) and phytase on growth performance, parameters of tibia and plasma, and meat quality of 1- to 21-d-old broilers. Two hundred and forty male, 1-d-old Arbor Acres broilers were randomly assigned to 20 cages, with 12 chicks per cage. Five treatments were designed, with four cages each. A 2${\times}$2 factorial experiment was designed to test 0 and 5 ${\mu}g/kg$ of $1{\alpha}$-OH $D_3$ in combination with 0 and 500 U/kg of phytase. A basal diet was formulated to contain 2.9 g/kg of non-phytate phosphorus (NPP), and the control diet was formulated to contain a normal level of NPP (4.5 g/kg). Results showed that $1{\alpha}$-OH $D_3$ alone increased tibia ash, contents of calcium and phosphate, breaking strength, concentrations of plasma calcium and phosphate, and water-holding capacity of breast and thigh meat, while it decreased growth of broilers. Phytase alone improved performance and tibia quality. Although growth of broilers was lower than that of the positive control when the diet was supplemented with $1{\alpha}$-OH $D_3$ and phytase, tibia quality was significantly improved by the addition of $1{\alpha}$-OH $D_3$ and phytase. These data suggest that interaction between $1{\alpha}$-OH $D_3$ and phytase at 2.9 g/kg of dietary NPP could significantly increase bone quality of 1- to 21-d-old broilers, while not improving growth performance.

환경조절에 의한 돌돔 Oplegnathus fasciatus 성 성숙 유도 (Sexual Maturation Inducement of Striped Knife-Jaw, Oplegnathus fasciatus by Manipulating Environmental Condition)

  • 김성연;방인철;김석민
    • 한국어류학회지
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    • 제12권1호
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    • pp.46-53
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    • 2000
  • 돌돔 Oplegnathus fasciatus의 인위적인 성 성숙 및 산란 유도를 위한 연구에서, 3년생 돌돔 친어를 대상으로 수온 및 광주기 조절에 의한 생식소 발달을 조직학적으로 조사하였다. 수온 및 광주기 조절구 (Exp. I)는 1996년 12월부터 1997년 2월까지 수온은 $14.5^{\circ}C$에서 $21.0^{\circ}C$ 그리고 광주기는 10 : 30 L에서 15 : 30 L로 증가시킨 후 4월까지 계속 유지시켰다. 수온 조절구 (Exp. II)에서 수온은 Exp. I과 동일하게 조절하였고, 광주기는 3월 초순까지는 자연 광주기로 조절하였다가 이후 장일 광주기 (15 : 30L)로 재조절 하였다. 대조구는 1996년 12월부터 1997년 4월까지 해상 가두리의 자연 조건하에서 사육하였다. 생식소 활성은 1 월부터 Exp. I, II 모두에서 시작되었다. 그리고 성숙과 산란은 Exp. I에서는 수온과 광 주기가 각각 $21.0^{\circ}C$와 15 : 30L까지 도달한 2월부터 시작되었으나, Exp. II는 3월 초순까지도 부분적 성숙만 유도되다 장일 광주기 (15 : 30 L)로 조절한 후 3월 하순 부터는 성숙과 산란이 시작되었다.

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The Effects of Rhodobacter capsulatus KCTC-2583 on Cholesterol Metabolism, Egg Production and Quality Parameters during the Late Laying Periods in Hens

  • Lokhandea, Anushka;Ingale, S.L.;Lee, S.H.;Kim, J.S.;Lohakare, J.D.;Chae, B.J.;Kwon, I.K.
    • Asian-Australasian Journal of Animal Sciences
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    • 제26권6호
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    • pp.831-837
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    • 2013
  • An experiment was conducted to investigate the effects of dietary supplementation of Rhodobacter capsulatus KCTC-2583 on egg-yolk and serum cholesterol, egg production and quality parameters during the late laying periods in hens. A total of 160 Hy-Line Brown layers (54 wk-old) were randomly allotted to 4 treatment groups on the basis of laying performance. Each treatment had 4 replicates with 10 birds each (40 birds per treatment). Two hens were confined individually with cage size $35{\times}35{\times}40$ cm and each 10 birds (5 cages) shared a common feed trough between them forming one experimental unit. Dietary treatments were; basal diet supplemented with 0 (control), 0.05, 0.10 and 0.15% R. capsulatus KCTC-2583. Experimental diets were fed in meal form for 56 d. Dietary supplementation of increasing levels of R. capsulatus KCTC-2583 reduced (linear, p<0.05) egg-yolk cholesterol and triglycerides (d 28, 42 and 56) concentrations. Also, serum cholesterol and triglycerides (d 21, 42 and 56) concentrations were linearly reduced (p<0.05) with increasing dietary R. capsulatus KCTC-2583. Laying hens fed a diet supplemented with increasing levels of R. capsulatus KCTC-2583 had increased (linear; p<0.05) overall egg production, egg weight, egg mass and feed efficiency. However, dietary treatments had no effect (linear or quadratic; p>0.05) on feed intake of laying hens. At d 28 and 56, breaking strength and yolk colour of eggs were linearly improved (p<0.05) in laying hens fed dietary increasing levels of R. capsulatus KCTC-2583. Dietary treatment had no effects (linear or quadratic; p>0.05) on albumin height, shell thickness and shell weight at any period of experiment. These results indicate that dietary supplementation of R. capsulatus KCTC-2583 has the potential to improve the laying hen performance and lead to the development of low cholesterol eggs during late laying period in Hy-Line Brown hens.

복합운동훈련이 신생 흰쥐의 알코올성 소뇌손상 후 운동기능 및 신경연접가소성에 미치는 영향 (The Effects of Complex Motor Training on Motor Function and Synaptic Plasticity After Neonatal Binge-like Alcohol Exposure in Rats)

  • 이선민;구현모;권혁철
    • 한국전문물리치료학회지
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    • 제12권3호
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    • pp.56-66
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    • 2005
  • The purposes of this study were to test that complex motor training enhance motor function significantly, to test change in cerebellum, and to test the synaptic plasticity into the immunohistochemistry response of synaptophysin. Using an animal model of fetal alcohol syndrome - which equates peak blood alcohol concentrations across developmental period - the effects of alcohol on body weight during periods were examined. The effect of complex motor training on motor function and synaptic plasticity of rat exposed alcohol on postnatal days 4 through 10 were studied. Newborn rats were assigned to one of two groups: (1) normal group (NG), via artificial rearing to milk formula and (2) alcohol groups (AG), via 4.5 g/kg/day of ethanol in a milk solution. After completion of the treatments, the pups were fostered back to lactating dams, where they were raised in standard cages (two-and three animals per cage) until they were postnatal 48 days. Rats from alcohol group of postnatal treatment then spent 10 days in one of two groups: Alcohol-experimental group was had got complex motor training (learning traverse a set of 6 elevated obstacles) for 4 weeks. The alcohol-control group was not trained. Before consider replacing with "the experiment/study", (avoid using "got" in writing) the rats were examined during four behavioral tests and their body weights were measured, then their coronal sections were processed in rabbit polyclonal antibody synaptophysin. The synaptophysin expression in the cerebellar cortex was investigated using a light microscope. The results of this study were as follows: 1. The alcohol groups contained significantly higher alcohol concentrations than the normal group. 2. The alcohol groups had significantly lower body weights than the normal group. 3. In alcohol groups performed significantly lower than the normal group on the motor behavioral test. 4. In alcohol-control group showed significantly decreased immunohistochemistric response of the synaptophysin in the cerebellar cortex compared to the nomal group. These results suggest that improved motor function induced by complex motor training after postnatal exposure is associated with dynamically altered expression of synaptophysin in cerebellar cortex and that is related with synaptic plasticity. Also, these data can potentially serve as a model for therapeutic intervention.

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Effect of calcium stearoyl-2 lactylate and lipase supplementation on growth performance, gut health, and nutrient digestibility of broiler chickens

  • Wickramasuriya, Samiru Sudharaka;Cho, Hyun Min;Macelline, Shemil Priyan;Kim, Eunjoo;Shin, Taeg Kyun;Yi, Young Joo;Park, Seung Hwan;Lee, Kyung Bon;Heo, Jung Min
    • Asian-Australasian Journal of Animal Sciences
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    • 제33권6호
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    • pp.981-991
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    • 2020
  • Objective: To evaluate calcium stearoyl-2 lactylate (CSL) performance as an exogenous emulsifier together with lipase for broiler diets. Methods: In total, 252 one-day-old Ross 308 broiler chickens were allocated in a completely randomized design to give 6 replications per treatment with 7 birds in each cage. There were six dietary treatments representing a 2×3 factorial arrangement consisted of two energy levels (standard energy [positive control, PC] and -100 kcal/kg of the requirement level [negative control, NC]) and three dietary treatments (without additives [CON], CON+CSL [CSL], and CON+CSL+lipase [CSL-Lipase]). Corn and soybean meal-based experimental diets containing vegetable oil were formulated. Growth performance, blood parameters, visceral organ weights, ileal morphology, nutrient digestibility, and cytokine gene expression were measured. Results: Birds fed a diet including CSL increased (p<0.05) lipase level in blood compared to birds fed a diet including CSL-Lipase on day 21. Similarly, higher (p<0.05) liver weight was observed in birds fed a diet including either CSL or CSL-Lipase on day 21. Birds fed NC diet with CSL improved (p<0.05) nutrient digestibility compared to the NC diet on day 21. However, birds fed a diet supplemented with CSL or CSL-Lipase did not affect (p>0.05) the weight gain, feed efficiency, ileal morphology, and cytokine concentrations during the experiment period, regardless of dietary energy levels. Conclusion: Our results indicated that CSL has a role in improving nutrient digestibility in young birds when supplemented to a corn-soybean meal based broiler diet.

Effect of Bacillus amyloliquefaciens-based Direct-fed Microbial on Performance, Nutrient Utilization, Intestinal Morphology and Cecal Microflora in Broiler Chickens

  • Lei, Xinjian;Piao, Xiangshu;Ru, Yingjun;Zhang, Hongyu;Peron, Alexandre;Zhang, Huifang
    • Asian-Australasian Journal of Animal Sciences
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    • 제28권2호
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    • pp.239-246
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    • 2015
  • The present study was conducted to evaluate the effect of the dietary supplementation of Bacillus amyloliquefaciens-based direct-fed microbial (DFM) on growth performance, nutrient utilization, intestinal morphology and cecal microflora in broiler chickens. A total of two hundred and eighty eight 1-d-old Arbor Acres male broilers were randomly allocated to one of four experimental treatments in a completely randomized design. Each treatment was fed to eight replicate cages, with nine birds per cage. Dietary treatments were composed of an antibiotic-free basal diet (control), and the basal diet supplemented with either 15 mg/kg of virginiamycin as antibiotic growth promoter (AGP), 30 mg/kg of Bacillus amyloliquefaciens-based DFM (DFM 30) or 60 mg/kg of Bacillus amyloliquefaciens-based DFM (DFM 60). Experimental diets were fed in two phases: starter (d 1 to 21) and finisher (d 22 to 42). Growth performance, nutrient utilization, morphological parameters of the small intestine and cecal microbial populations were measured at the end of the starter (d 21) and finisher (d 42) phases. During the starter phase, DFM and virginiamycin supplementation improved the feed conversion ratio (FCR; p<0.01) compared with the control group. For the finisher phase and the overall experiment (d 1 to 42) broilers fed diets with the DFM had better body weight gain (BWG) and FCR than that of control (p<0.05). Supplementation of virginiamycin and DFM significantly increased the total tract apparent digestibility of crude protein (CP), dry matter (DM) and gross energy during both starter and finisher phases (p<0.05) compared with the control group. On d 21, villus height, crypt depth and villus height to crypt depth ratio of duodenum, jejunum, and ileum were significantly increased for the birds fed with the DFM diets as compared with the control group (p<0.05). The DFM 30, DFM 60, and AGP groups decreased the Escherichia coli population in cecum at d 21 and d 42 compared with control group (p<0.01). In addition, the population of Lactobacillus was increased in DFM 30 and DFM 60 groups as compared with control and AGP groups (p<0.01). It can be concluded that Bacillus amyloliquefaciens-based DFM could be an alternative to the use of AGPs in broilers diets based on plant protein.

Effects of replacing soybean meal with perilla seed meal on growth performance, and meat quality of broilers

  • Oh, Han Jin;Song, Min Ho;Yun, Won;Lee, Ji Hwan;An, Ji Seon;Kim, Yong Ju;Kim, Gok Mi;Kim, Hyeun Bum;Cho, Jin Ho
    • Journal of Animal Science and Technology
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    • 제62권4호
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    • pp.495-503
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    • 2020
  • The purpose of this study was to evaluate effects of replacing soybean meal with perilla seed meal (PSM) on growth performance, proximate composition of meat, meat quality, and fatty acids composition of meat in broilers. A total of 60 one-day-old chicks of ROSS 308 (initial body weight of 44.8 ± 0.4 g) were randomly allotted to four treatment groups (15 replicate cages with 1 broilers per cage) in a period of 5 weeks. Dietary treatments included control (CON) basal diet (corn-soybean meal-based diets) and test diets in which PSM was included at 0.5% (T1), 1% (T2), or 2% (T3) to gradually replace soybean meal. At the end of experiment, all broilers were slaughtered for collecting breast and thigh meat. At 5 weeks, T2 and T3 treatment groups had higher (p < 0.05) body weights than CON and T1 treatment groups. Weight gain was increased (p < 0.05) in T2 and T3 treatment groups. Feed intake was decreased (p < 0.05) in T3 treatment group. Broilers in T2 treatment group had lower (p < 0.05) feed conversion ratio than those in other treatment groups. In proximate composition of breast meat, there was no significant (p > 0.05) difference in moisture and ash. Fat composition of breast meat was significantly (p < 0.05) increased in T2 and T3 treatment groups. Regarding meat quality, broilers fed T3 diet had higher (p < 0.05) water holding capacity than those fed other diets. Regarding fatty acids composition of thigh meat, broilers fed T3 diet had higher (p < 0.05) linolenic acid than those fed other diets. In conclusion, soybean meal replaced by 2% perilla seed meal in broiler diet can improve growth performance, meat quality, and fatty acids composition of thigh meat in broilers. Perilla seed meal can be a lot of potential alternatives feedstuff for soybean meal in this study.

직립식 산란계사 내의 소음 진동 발생 현황 조사연구 (Study on the Characteristics of Noise/Vibration in the Upright Laying Hen House)

  • 이승주;장동일;장홍희
    • 한국축산시설환경학회지
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    • 제12권1호
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    • pp.21-28
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    • 2006
  • 국민의 소득 증대로 인해 육류 및 계란의 소비량이 점차적으로 증가하는 추세에 있다. 이로 인해 산란계사의 규모가 대형화됨에 따라 기계화되었고, 이 연구에서는 고단 직립식 케이지를 사용하는 무창계사에서 기계시설이 작동 시 발생되는 소음 및 진동이 산란계의 생산성에 미치는 영향을 구명하고자 산란계사내의 소음/진동 수준을 측정 분석하였다. 전국의 13개 산란계 농장을 대상으로 하여 평상시 및 기계시설(사료급이기, 사료분배기, 집란시스템, 환기장치, 계분벨트)의 작동시에 발생되는 소음/진동을 측정하였다. (1) 평상시 소음 최대 82dB, 진동 최대 0.2072 cm/s, (2) 사료급이기 작동 시 소음 최대 90dB, 최대 진동 2.8560cm/s, (3) 사료분배기 작동시 소음 최대 90dB, 최대 진동 2.022cm/s, (4) 집란시스템 작동 시 최대 소음 87dB, 최대 진동 0.1865cm/s, (5) 환기장치 작동 시 케이지에 미치는 최대 88dB, 계사 후면 벽면에 미치는 최대 진동 2.5364cm/s, (6) 계분벨트 작동 시 최대 소음 88dB, 최대 진동 0.2387cm/s 이었다. 따라서 산란계에게 직접적이고 가장 크게 영향을 미치는 장치는 급이 시스템이였다. 이 때의 소음은 최대 90dB로 가장 높게 발생하였으며, 진동은 최대 2.8560 cm/s 발생하였다.

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유황을 급여한 육용 교잡계의 성장능력과 계육의 물리.화학적인 성상의 비교 (A Comparison of Fattening Performance, Physico-Chemical Properties of Breast Meat, Vaccine Titers in Cross Bred Meat Type Hybrid Chicks Fed Sulfur)

  • 박재홍;류명선;이영은;송근섭;류경선
    • 한국가금학회지
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    • 제30권3호
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    • pp.211-217
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    • 2003
  • 본 실험은 유황을 육용 교잡계에 급여하여 성장능력, 계육의 물리$.$화학적인 성상, 항체가, 관능검사에 미치는 영향을 구명하고자 실행하였다. 8주령 교잡 육용계를 케이지에 암수 1:1의 비율로 처리구당 4반복으로 반복당 18수씩 총 288수를 배치하여 3주간 사양실험을 실행하였다. 유황은 황 함유량이 45%제재로서 0, 1.0, 2.0, 4.0%를 시판용 사료에 첨가하여 급여하였다. 증체량, 사료섭취량, 사료요구율은 주간별로 측정하여 계산하였으며, 실험 종료시에 계육의 일반성분, 물리$.$화학적인 성상, 백신항체가, 관능검사를 실행하였다. 유황 급여구에서 증체량, 사료섭취량, 사료요구율은 대조구와 통계적인 차이가 없었지만 복강지방은 대조구에 비하여 증대되는 경향을 보였다. 가슴육의 지방함량은 유황급여구가 대조구에 비하여 매우 낮았으며(P<0.05), 적색도가 높아지는 경향을 나타냈다. 조리감량은 유황 급여구에서 대조구에 비하여 현저하게 낮았으며(P<0.05), 조직감 특성중 응집성, 탄력성 및 검성은 증가하는 경향을 나타냈다. 관능평가 결과 유황을 첨가함에 따라 다즙성은 증가하고, 기름이 있는 입촉감은 감소하는 경향을 보여(P<0.05) 유황 첨가가 닭고기의 수용도에 좋은 영향을 주는데 기여하였으며, 씹힘성은 유황 첨가수준 2%에서 가장 연한 것으로 나타났으며(P<0.01) 닭고기에 대한 전체적인 수용도는 유황 2% 첨가구에서 통계적인 차이를 보였으므로 가장 좋은 것으로 평가되었다. 유황의 급여로 혈액의 AST, ALT, BUN은 대조구와 차이가 없었다.

생리적 요구에 따른 인의 시간별 급여가 산란계의 능력에 미치는 영향 (Effects of Feeding Laying Hens Different Levels of Dietary Phosphorus at Different Physiological Status)

  • 유경선;최진호
    • 한국가금학회지
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    • 제13권1호
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    • pp.15-21
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    • 1986
  • 산란계에서 인의 생리적인 요구에 맞추어 인 수준이 다른 사료를 서로 다른 시간에 급여하였을 때 산란율, 사료섭취량, 사료효율, 인, 섭취량, 산란시각, 계란의 비중, 란중에 미치는 영향과 란중과 산란시각, 계란의 비중과 산란시각, 계란의 비중과 란중의 관계를 조사하기 위하여 108수의 Manina Brown 산란계를 공시하여 14일간 시험한 결과는 다음과 같다. 1. 0.7% 인 수준의 사료를 계속 급여한 대조구(처리 1)와 오전 6시부터 10시까지 2.54%의 인 수준의 사료를 급여하고 나머지 시간에 0.33% 인 수준의 사료를 급여한 처리 2와 2.54% 인 수준의 사료를 산란직후 4시간 동안 급여하고 나머지 시간에는 0.33% 인 수준의 사료를 급여한 처리 3과 사이에 산란율, 사료효율은 차이가 없었으나 (p〈0.01) 인 섭취량은 처리 1에 비하여 처리 2와 3에서 높았다(P〈0.01). 2. 산란시각은 처리3이 제일 빨랐으며 처리 1(P〈0.05) 및 처리 2(P〈0.01)와 유의차가 있었다. 3. 평균 란중은 사료의 인 수준을 달리하여 급여시 처리간에 별 다른 차이를 보이지 않았다. 4. 계란의 비중은 처리 3에서 처리1이나 2에 비하여 높았으나 (P〈0.01) 처리 1과 처리 2 사이에는 유의차가 없었다. 5. 산란시각과 란중의 관계는 부(-)의 상관관계를 보였는데, 아침 일찍 산란한 계란은 오후에 산란한 계란보다 무거웠음을 나타내었다. (P〈0.01). 6. 산란시각과 계란의 비중사이에는 2차 회귀관계가 인정되었으며, 늦게 산란한 계란일수록 비중은 높았다. (P〈0.01). 7.계란의 비중과 란중의 관계는 부(-)의 상관관계를 보였으며, 란중이 커질수록 계란의 비중은 저하되는 경향을 나타내었다. (P〈0.01).

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