• Title/Summary/Keyword: byc1

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Variation of Anthocyanins and Isoflavones between Yellow-Cotyledon and Green-Cotyledon Seeds of Black Soybean

  • Kim, Sun-Lim;Kim, Hyun-Bok;Chi, Hee-Youn;Park, Nam-Kyu;Son, Jong-Rok;Yun, Hong-Tae;Kim, Si-Ju
    • Food Science and Biotechnology
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    • v.14 no.6
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    • pp.778-782
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    • 2005
  • Analysis of black soybeans [Glycine max (L.) Merr.; 59 Korean varieties] revealed that 100-seed weights of green cotyledon seeds (33.5 g, n=31) were higher than those of yellow ones (28.9 g, n=28). Contents of delphinidin-3-glucoside (D3G), cyanidin-3-glucoside (C3G), petunidin-3-glucoside (P3G), and total anthocyanins in seed coats of black soybeans were 0.03-4.15, 0.74-18.36, 0.02-1.60, and 0.87-23.52 mg/g, respectively, among which most prominent anthocyanin was C3G (80.9% of total content), followed by D3G (13.6%) and P3G (5.5%). No significant differences were observed in color parameters $a^*$ and $b^*$ between black soybeans with yellow cotyledon (BYC) and green cotyledon (BGC). Total isoflavone content of BGC was higher than that of BYC, and negative correlation was found between total anthocyanin and isoflavone contents.

High Frequency Somatic Embryogenesis and Plant Regeneration in Seedling Explant Cultures of Melon (Cucumis melo L.) (멜론(Cucumis melo L.) 유묘 절편으로부터 고빈도의 체세포배발생과 식물체 재분화)

  • 최필선;소웅영;조덕이;유장렬
    • Korean Journal of Plant Tissue Culture
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    • v.21 no.1
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    • pp.1-6
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    • 1994
  • Cotyledonary and hypocotyl explants of melon seedlings were cultured on Murashige and Skoog's (MS) medium supplemented with various concentrations of 2,4-Dichlorophenoxyacetic acid (2,4-D) and benzyladenine (B.A).Up to 22% of cotyledonary explants and 7%, of hypocotyl explants, respectively: Produced somatic embryos through intervening two types of calli: bright yellow compact (BYC) callus and pale-yellow compact (PYC) callus. BYC callus was capable of producing somatic embryos at initial culture, but it became necrotic as subrulhues proceeded. In contrast UC callus was incapable of producing somatic embryos during initial culture (first 6 weeks), but it became bright-yellow friable (BYF) callus with forming a few globular embryos after 2 months of subculture, indicating that the callus turned embryogenic. The embryogenic capacity of BYF maintained for over one year when the callus was sucultured at 4-week interval. Upon transfer onto MS basal medium the callus gave rise to numerous somatic embryos and subsequently converted to plantlets. Plantlets were transplanted to potting soil and grown to maturity in the phyotron.

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Preparation of Wool/Poly(ethylene terephthalate) Composite Membrane and It's Dyeablities (모/폴리에틸렌테레프탈레이트 복합막의 제조와 염색성)

  • Kim, Gong-Ju;Shin, Hye Kyong;Park Mi-Ra;Kim, Kyong-Hi;Jeon, Jae-Hong
    • Textile Coloration and Finishing
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    • v.9 no.1
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    • pp.23-32
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    • 1997
  • Composite membranes having different mixing ratio of Wool(SCMK) and poly(ethylene terephthalate) (PET) were prepared by dissolving wool/PET in hexafluoro-2-propanol(HFIP), casting the obtained solution on a glass plate and evaporation the solvent in the presence and absence of an electric field. The internal structure of the prepared membrane was investigated using polarise microscope dyeing and dye permeation method. In the composite membrane prepared under electric field, both components were micro mixing, while in the membranes prepared under nonelectric field, the two components formed a random sea/island structure according to different mixing ratio. Such characteristic membrane structure was influenced the permeation behavior of C.I. Acid Red 118 through the membranes from an aqueous solution.

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Novel strategy for isolating suppressors of meiosis-deficient mutants and its application for isolating the bcy1 suppressor

  • Shin, Deug-Yong;Yun, Jean-Ho;Yoo, Hyang-Sook
    • Journal of Microbiology
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    • v.35 no.1
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    • pp.61-65
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    • 1997
  • A novel strategy was developed for isolating suppressors from sporulation-deficient mutants. The mutation in the BCY1 gene, which codes for the regulatory subunit of cAMP-dependent protein kinase, when homozygous, results in diploids being meiosis and sporulation deficient. Two plasmids, YCp-MAT.alpha. and YEp-SPOT7-lacZ, were introduced into MAT.alpha. BCY1$\^$+/ or MAT.alpha. bcy1 haploid cells. The transformant of the BCY1$\^$+/ haploid cell produced .betha.-galactosidase under nutrient starvation, but the bcy1 transformant did not. Using this system, the mutagenesis experiment performed on the bcy1 transformant strain resulted in a number of sporulation mutants that produced .betha.-galactosidase under nutrient starvation. One complementation group, sob1, was identified from the isoalted suppressor mutants and characterized as a single recessive mutation by tetrad analysis. Genetic analysis revealed that the sob1 mutation suppressed the sporulation deficiency, the failure to arrest at the G1 phase of the cell cecle, and the sensitivity to heat or nitrogen starvation caused by the bcy1 mutation. However, the sob1 mutation did not suppress the sporulation deficiency of ime1 and of ime2 diploids. These results suggest that the sob1 mutation affects a gene which functions as a downstream regulator in both meiosis and cell cycle regulation.

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Analysis of Korean Consumer Brand Awareness and Preferred Types of Sock Design (국내 양말 소비자의 브랜드 인지도 및 디자인 선호도 분석)

  • Kim, Chil-Soon;Nam, Young-Mi;Kim, Hyun-Su
    • Fashion & Textile Research Journal
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    • v.1 no.4
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    • pp.335-341
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    • 1999
  • The purpose of this study was to develop a large representative data base for socks marketing strategy. The study was to determine socks brand awareness in ralation to segmented distribution regions and such demographic variables as sex and age. The authors also analyzed preferred design. A total of 650 questionnaires were distributed and 611 reliable ones were used for statistical analysis. A SAS statistical package including frequency tables and chi square test was used. The results are as follows: Brand awareness involves "brand recall" based on asking a person to name the brand he or she recalls first, and also "brand recognition" based on asking subjects to identify brand names from 30 given brands. 'SOCKSTOP' was found to be a dominant brand as a result of the brand recall test, and 'BYC' was found to be a dominant brand as a result of the brand recognition test. Brand recognition was significantly different in the segmented distribution regions, three age groups and different sex groups. People considered design first in purchasing sports/casual socks, while they considered color first in purchasing dress socks. The most favored type of sock style was the common crew sock type. The results of a chi square test showed that preferred type of sock design was related with the sex and age variables.

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