• Title/Summary/Keyword: burial test

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Characterization of Bacterial Community Dynamics during the Decomposition of Pig Carcasses in Simulated Soil Burial and Composting Systems

  • Ki, Bo-Min;Kim, Yu Mi;Jeon, Jun Min;Ryu, Hee Wook;Cho, Kyung-Suk
    • Journal of Microbiology and Biotechnology
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    • v.27 no.12
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    • pp.2199-2210
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    • 2017
  • Soil burial is the most widely used disposal method for infected pig carcasses, but composting has gained attention as an alternative disposal method because pig carcasses can be decomposed rapidly and safely by composting. To understand the pig carcass decomposition process in soil burial and by composting, pilot-scale test systems that simulated soil burial and composting were designed and constructed in the field. The envelope material samples were collected using special sampling devices without disturbance, and bacterial community dynamics were analyzed by high-throughput pyrosequencing for 340 days. Based on the odor gas intensity profiles, it was estimated that the active and advanced decay stages were reached earlier by composting than by soil burial. The dominant bacterial communities in the soil were aerobic and/or facultatively anaerobic gram-negative bacteria such as Pseudomonas, Gelidibacter, Mucilaginibacter, and Brevundimonas. However, the dominant bacteria in the composting system were anaerobic, thermophilic, endospore-forming, and/or halophilic gram-positive bacteria such as Pelotomaculum, Lentibacillus, Clostridium, and Caldicoprobacter. Different dominant bacteria played important roles in the decomposition of pig carcasses in the soil and compost. This study provides useful comparative date for the degradation of pig carcasses in the soil burial and composting systems.

Experimental Assessment for the Effect of Burial Depth on the Formation of Underground Cavities and Ground Cave-ins by Damaged Sewer Pipes (하수관 손상으로 인한 지하공동 및 지반함몰 발생에 대한 하수관 매립심도 영향의 실험적 평가)

  • Kwak, Tae-Young;Chung, Choong-Ki;Kim, Joonyoung;Lee, Minho;Woo, Sang-Inn
    • Journal of the Korean Geotechnical Society
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    • v.35 no.11
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    • pp.37-49
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    • 2019
  • In order to analyze the effect of burial depth on the generation of ground cavities and cave-ins, a series of model experiments with different height of model ground were performed. Digital images of the model ground were captured to evaluate the internal deformation of the model grounds by adopting the PIV (Particle Image Velocimetry) technique. Additionally, the vertical displacement at the surface, the size of the cavity, and the weight of the discharged soil were measured in each test. The results indicate that the model ground with low burial depth, which does not satisfy the criterion, was more vulnerable to ground cavities and cave-ins than the model ground with high burial depth.

Biodegradation Characteristics of Poly(butylene succinate-co-butylene adipate) during Soil Burial Test (토양 매립 시험에서 Poly(butylene succinate-co-butylene adipate)의 생분해 특성)

  • Kim, Mal-Nam
    • Korean Journal of Environmental Biology
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    • v.28 no.3
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    • pp.150-157
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    • 2010
  • Biodegradation behavior of poly(butylene succinate-co-butylene adipate) (PBSA) was examined when PBSA was buried in the natural soil and the soil inoculated with Burkholderia cepacia after sterilization. After 80 days of the soil burial test at room temperature, the PBSA film buried in the natural soil lost 34.0% of its intial weight, while the same film lost 59.2% of its initial weight when buried in the sterile soil inoculated with B. cepacia. The optical and SEM observations of the surface morphology of the PBSA film also indicated that the surface erosion and rupture took place faster when the film was buried in the sterile soil inoculated with B. cepacia compared to the film buried in the natural soil. Viable cell number in the natural soil and that the sterile soil inoculated with B. cepacia increased by a factor of 6~7 and 10~14, respectively as compared to the initial viable cell number.

A Study on the Biodegradable Properties of Polyesters associated with their Chemical Structures (폴리에스테르의 화학적 구조에 따른 생분해 거동에 관한 연구)

  • Woo, Je-Wan;Sohn, Myung-Ho;Cha, Hye-Young;Park, Yang-Sung;Chang, Kil Sang;Whang, Young-ae;Park, Sang-Soon
    • Clean Technology
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    • v.8 no.4
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    • pp.223-228
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    • 2002
  • The biodegradable Properties of various polyester resins with different chemical structures have been studied by applying the controlled compost test and soil burial test. Celluose was taken as a fully biodegradable reference resin while PVC and PE were empolyed as non-biodegradable reference chains or ester group were rather easily degraded by hydrolase, meanwhile copolymer type polyesters which contain aromatic rings showed relatively low biodegradability. According to the results from controlled compost test, cellulose(the positive reference) showed 70.6% degradation after 45 days, whereas synthetic poly(butylene adipate-co-succinate), poly(butylene succinate), poly(butylene adipate-co-succinate-co-terephthalate) showed 44.0%, 32.0% and 23.4% degradation respectively. In this regard, it was concluded that biodegradable properties of polymers are largely dependant on the chemical structures constituting the polymers.

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Analyses on Artifacts of Koryo Kynasty Excavated from Hyungok-ni Danyang (단양 현곡리 출토 고려시대 섬유류 및 지류 유물에 관한 연구)

  • 안춘수;박희현
    • Journal of the Korean Society of Clothing and Textiles
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    • v.23 no.8
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    • pp.1161-1169
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    • 1999
  • This research was aimed to identify the artifact type and their characteristics of the textile related materials excavated from Koryo Dynasty tombs of Hyungok-ni Danyang. Microscopy solubility test energy dispersive X-ray spectroscopy(EDS) and fourier transform infrared spectroscopy (FT-IR) fabric density test were used for the identification and when necessary standard samples were made in order to compare its characteristics with hose of the original artifact, the result indicated that the artifacts included both textile and paper. Artifacts from Burial 14 were comprised of textile fragments FT-IR and the solubility test conducted on the artifact indicated that the textiles were made with ramie woven with plain weave technique in a fairly loose fabric density. Rest of the artifacts were all comprised of paper.

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Analysis of Microbiological Contamination in the Chosun Dynasty Textiles Exhumed from Hwasung Kupori Burial

  • Cheunsoon Ahn;Kim, Jung-wan
    • The International Journal of Costume Culture
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    • v.2 no.1
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    • pp.54-61
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    • 1999
  • The purpose of this research was first to analyse and compare the types of microbes inherent among the unwashed and washed Kupori textiles, and second to investigate whether there is a difference between unwashed and washed Kupori textiles on the susceptibility of contamination by microorganism when exposed to the same microbial environment. Microbial identification procedure and the Shake Flask Test for investigating the effect of exposure to microbial environment were carried out separately. The result of microbial identification procedure indicated that a variety of bacteria and fungi were inherent in both unwashed and washed textiles and that the population of contaminated microorganism became more diverse after washing. The result of Shake Flask Test indicated that given the same exposure condition, the unwashed textiles tend to be more susceptible to bacterial contamination than the washed textiles. The results of the present study supported the current conservation procedures adopted in Korean museums which include washing and humigation procedures before long-term storage or display of exhumed textiles.

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The Design and Implementation of a Real-Time FMD Cattle Burial Sites Monitoring System Based-on Wireless Environmental Sensors (u-EMS : 센서네트워크 기반의 가축매몰지 악취환경정보 실시간 모니터링 시스템 설계 및 구현)

  • Moon, Seung-Jin;Kim, Hong-Gyu;Park, Kyu-Hyun
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.36 no.12B
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    • pp.1708-1721
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    • 2011
  • Recent outbreak of cattle diseases such as foot-and-mouth disease(FMD) requires constant monitoring of burial sites of mass cull of cattles. However, current monitoring system takes environmental samples from burial sites with period of between one and two weeks, which makes it impossible for non-stop management of hazardous bio-waste. Therefore, in this study, we suggest an improved real-time environmental monitoring system for such bio-hazardous sites based on wireless sensor networks, which makes constant surveillance of the FMD burial sites possible. The system consists mainly several wireless environmental monitoring sensors(i.e dust, Co2, VOC, NH3, H2S, temperature, humidity) nodes and GPS location tracking nodes. Through analysis of the relayed of the environmental monitoring data via gateway, the system makes it possible for constant monitoring and quick response for emergency situation of the burial sites. In order to test the effectiveness of the system, we have installed a set of sensor to gas outlets of the burial sites, then collected and analyzed measured bio-sensing data. We have conducted simulated emergency test runs and was able to detect and monitor the foul smell constantly. With our study, we confirm that the preventive measures and quick response of bio environmental accident are possible with the help of a real-time environmental monitoring system.

Comparison of Behaviors for Underground Flexible Pipes with Installation Gap (관로 이격거리에 따른 지중매설관의 거동특성 비교)

  • 이대수;상현규;김경열;홍성연
    • Journal of the Korean Geotechnical Society
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    • v.19 no.5
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    • pp.79-87
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    • 2003
  • Underground flexible pipes for electric cables are subject to external loads and surrounding soil pressure. Particularly, strain of flexible pipes is of great concern in terms of safety and maintenance of electric cables. In this paper, stress and strain of flexible pipes with two types of installation gap, ie, l0cm and naught, were compared to investigate the structural integrity of pipes from actual field test. The effect of degree of compaction and burial depth were also investigated to simulate the variety of construction situation. The results of the field test show that the strain criteria were satisfied under the burial depths of 80cm, 100cm and 120cm regardless of installation gap.

Biodegradation of Cotton/Polyester Blends (면/폴리에스터 혼방직물의 생분해성 평가)

  • Lee, Seung-Hyun;Park, Chung-Hee;Im, Seung-Soon
    • Journal of the Korean Society of Clothing and Textiles
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    • v.29 no.2
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    • pp.347-355
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    • 2005
  • Biodegradability of cotton/polyester blend fabric was investigated employing activated sluge test, soil burial test and enzyme hydrolysis. Surface changes of the degraded sample were observed through a microscopy. Changes in X-ray diffraction patterns and crystallinity were examined using X-ray diffractometer. Experimental results revealed that biodegradability of cotton/polyester blend fabric was proportional to the blending ratio of cotton, not showing any synergy effect. Polyester 100% hardly degraded in this study. Through the comparison of the experimental method it was shown that the biodegradabilities determined from activated sludge test and enzymatic hydrolysis except soil burial test were linearly related to the blending ratio of cotton in the blent fabrics. It is probably because the biodegradability determined from the retention of tensile strength of fabrics buried in soil was affected by the stress distribution of polyesters throughout the fabric. From the microscopic observations it was revealed that fungi were grown on the fabric surface and the colors turned yellow, brown and black. X-ray diffraction patterns showed that the heights of crystalline peak coming from cotton part in blend fabrics decreased whereas those coming from polyester part increased comperatively as time passed by. Crystallinities of cotton 100% fabric increased slightly at the begining and then decreased continuously.

Biodegradabilities of Cotton Fabrics treated with Silicones (실리콘 처리한 면직물의 생분해성)

  • 김보형;박정희;임승순
    • Journal of the Korean Society of Clothing and Textiles
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    • v.28 no.8
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    • pp.1048-1056
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    • 2004
  • Hydrophilicities of finished cotton fabrics were evaluated in respect of moisture regain and wickability. Changes in internal structure were determined using X-ray diffraction and surface changes in degraded samples were observed through a microscopy. Activated sludge test, soil burial test and enzyme hydrolysis were employed to evaluate the biodegradabilities. In addition, correlation analysis was done between biodegradability and the factors affecting biodegradability in each evalution methods. It was shown that hydrophilicities of silicone finished specimens were lower than that of untreated cotton and decreased in a row of PDMS(polydimethyl siloxane : -CH$_3$)>AFS(amino functional siloxane ; -C$_3$H$_{6}$ NHC$_2$H$_4$NH$_2$)>MHPS(methylhydrogen polysiloxane : -H, Cat : (C$_{17}$ H$_{35}$ COO)$_2$Zn) Although, moisture regain of mercerized cotton was higher than those of the others, wickability was shown to be lower. It was represented that crystalinities of cotton fabrics decreased by the silicone treatment. In activated sludge test and soil burial test, biodegradabilities of silicone treated specimens were lower than that of untreated cotton, where specimens of higher biodegradability exhibited higher biodegradability except mercerized ones. The results from enzyme hydrolysis, however, showed somewhat different tendency in that biodegradability was more closely related with the crystallinities of fabrics. It can be thought that enzyme hydrolysis is carried out for short time, physical accessibility becomes important.