• Applied Biological Chemistry
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• 제16권1호
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• pp.12-17
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• 1973

냉동시킨 마늘로부터 Alliin을 분리(分離)해서 순수한 상태(相態)로 결정화(結晶化) 시켰다. 분리(分離)된 Alliin의 순도(純度)는 융점측정과 thin-layer chromatography에 의하여 확증되었다. Sulfoxide bond 및 아미노산의 기능기는 IR spectrum에 의하여, vinyl 결합은 NMR spectrum에 의하여 확인되었다. 분자량, allyl 및 기타의 결합은 MS spectrum에 의하여 확인(確認)되었다.

• 식물병연구
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• 제29권1호
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• pp.39-44
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• 2023

To diagnose lily fasciation, lily bulbs showing fasciation were collected from several greenhouses in Jeju Island, South Korea. Bacteria were isolated from the lily bulbs and amplified with both primers for fasA in plasmid and for putative glycosyltransferase epsH gene in chromosome of Rhodococcus fascians. Three bacterial isolates were detected with the P450 primer set and identified as R. fascians by NCBI blast analysis. Twelve bacterial isolates were identified as R. fascians using RS02785 primer set, including the three bacterial isolates identified as the same pathogen using the P450 primer set. Pathogenicity of these bacterial strains identified as R. fascians was demonstrated. Apparent symptoms were observed on wounded lily leaves after inoculation with each bacterial suspension whereas no symptom was found on lily leaves treated with H₂O. Furthermore, bacteria re-isolated from wounded sites were identified as R. fascians. Based on the results, these two sets of primers are recommended for quarantine of R. fascians.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2012년도 춘계학술대회 논문집
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• pp.983-984
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• 2012

• 농업과학연구
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• 제43권1호
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• pp.28-32
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• 2016

This experiment was carried out to determine optimal culture conditions for the production of cut flowers and tubers of Calla (Zantedeschia 'Black Magic') in highlands. To achieve the research purpose, growth of 'Black Magic' Calla influenced by tuber hardness (3.3 and $6.0kg/cm^2$), $GA_3$ concentration (0, 100 and $200mgL^{-1}$), duration of $GA_3$ treatment (24 hr, 12 hr and 30min before planting) were investigated. When tubers have high hardness, those were not severely injured by soft rot disease regardless of $GA_3$ concentrations and treatment durations. Tubers with low tuber hardness showed more than 90% of soft rot occurrence when treated with $200mgL^{-1}$ $GA_3$ for 24 hrs before planting. However, soft rot did not occur when tubers were treated with $200mgL^{-1}$ $GA_3$ for 12 hrs before planting. In conclusion, $GA_3$ treatment results showed soft rot occurrence statistically significant degree in accordance with the bulbs hardness. In addition, the yield of the cut flowers is the result received the greatest effect in accordance with the bulbs in size and appeared to not be determined in accordance with the $GA_3$ treatment concentration and hardness bulbs. To obtain flowers without soft rot symptom, tubers (as $6.0kg/cm^2$) should be completely dried after $GA_3$ treatment.

• 식물조직배양학회지
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• 제22권1호
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• pp.53-57
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• 1995

수선화의 기내증식에 미치는 TDZ처리의 가능성을 조사하기 위하여 disk를 붙인 인편을 MS 기본배지에 NAA, BA 및 TDZ을 첨가한 배지에 배양하였다. 인편배양으로부터 자구의 형성 및 직접적인 신초형성에는 5.0 mg/L NAA와 1.0 mg/L BA가 반응이 양호했고 3 mg/L NAA와 0.02 mg/L TDZ을 혼용처리했을 때에도 비교적 양호한 반응을 보였다. 분화된 유식물의 뿌리형성에는 5.0 mg/L NAA와 0.02 mg/L TDZ이 첨가된 배지에서 가장 양호했다. 기내배양에서 얻어 진 식물체의 조직편을 이용하여 기내증식에 미치는 시토키닌의 영향을 조사했던 바 TDZ이 다른 시토키닌보다 효과가 있었고 조직은 인편을 배양했을 때가 자구형성 및 신초 분화가 가장 양호했으며 1.0 mg/L NAA와 0.5 mg/L TDZ을 첨가한 배지에서 여러 조직편으로부터 자구형성 및 신초분화가 가장 양호하여서 수선화의 기내증식 시 TDZ처리의 가능성이 있음이 인지되었다.

• 식물조직배양학회지
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• 제27권1호
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• pp.25-29
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• 2000

백합의 건전 우량주의 확보와 대량 증식을 위해 소인편을 배양하여 얻어진 직경 3mm 정도 크기의 소인경구를 사용하여 액체배양을 실시하였다. 인경구의 생장에 미치는 sucrose와 질소 농도의 영향을 알아본 결과 인경구의 생장과 무게에 가장 효과적인 처리구는 10 mM $NH_4NO_3$에 sucrose 9% 또 는 20 mM $NH_4NO_3$에 sucrose 6%인 것으로 나타났으며, 새로운 소인경구의 분구는 $NH_4NO_3$, 20 또는 40 mM에 sucrose 3%에서 가장 양호하였다. 또한 전체 생체중은 광도 $60\;{\mu}mol{\cdot}m^2{\cdot}sec^{-1}$ BA 0.2 mg/L 단독 처리구에서 효과적이었으며, 구중은 BA에 농도에 큰 차이없이 0.2와 2 mg/L 처리에 광도 $60\;{\mu}mol{\cdot}m^2{\cdot}sec^{-1}$에서 증가하는 경향을 보였다. 액체 진탕배양에 적절한 소인경구의 배양기간은 2개월 정도인 것으로 나타냈다.

• 한국잡초학회지
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• 제31권4호
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• pp.330-339
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• 2011

연구는 붉노랑상사화 인경에 함유된 살초활성 물질을 분리하여 살초활성을 평가하고 그 화학구조를 구명하고자 수행되었다. Silica gel flash column chromatography, preparative TLC, HPLC 등 일련의 크로마토그래피법들을 통해 붉노랑상사화 인경의 MeOH 추출물을 분리 정제하였다. HPLC에 의해 최종 획득된 활성분획에 대한 GC/MS 분석 결과, galanthine (galanthan-1-ol)과 isoquinoline alkaloid인 montanine ($O^2$-methyl pancracine)이 동정되었다. Montanine은 $20{\mu}g\;mL^{-1}$ 농도에서 돌피 유묘의 생장을 거의 100% 억제시키는 것으로 나타났다. 한편, 붉노랑상사화 인경의 MeOH 추출물은 $1,000{\mu}g\;mL^{-1}$ 에서 대조구에 비해 화영벼 유묘의 신초와 유근의 생장을 각각 약 3.1% 및 8.3% 억제하였다.

• 한국생물환경조절학회:학술대회논문집
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• 한국생물환경조절학회 2001년도 봄 학술발표논문집
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• pp.45-50
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• 2001

Stem firmness and flowering response of cut lily as influenced by medium composition (Control: Upland soil, Pt: Peatmoss, Pe: Perlite, Ve: Vermiculite, Rrh: Rotted rice-hull, RPt: Russian Peatmoss) were studied. For 'Casa Blanca', plant height and length of flower stalk increased when bulbs were planted in Pt:Rrh:Ve(1:1:1, v/v), and dried leaves of lower part plants decreased by RPt:Pe:Rrh(1:1:1, v/v). In case of 'Marco Polo' plant height and length of flower stalk increased with Pt:Rrh(1:1, v/v) as compared to other treatment, number of leaves and dried leaves increased when bulbs were planted in RPt:Pe:Rrh(1:1:1, v/v) as compared to control. Flowering of 'Casa Blanca' was promoted in Pt:Pe:Ve(1:1:1, v/v) and 'Marco Polo' was accelerated in Pt:Rrh:Ve(1:1:1, v/v). Flower length of 'Casa Blanca' was increased by RPt:Pe:Rrh(1 :1 :1, v/v) as compared with control and 'Marco Polo' was increased when bulbs planted to Pt:Rrh(1 :1, v/v). Flower-bud blasting of two cultivars was increased with Pt as compared with other treatment. Stem firmness of 'Casa Blanca' was increased by Pt:Pe:Ve (1:1 :1, v/v), and especially, stem firmness of upper part plants was increased by Pt:Rrh(1 :1, v/v) in 'Marco Polo' as compared to control plants. but generally, stem firmness of 'Casa Blanca' was not influenced with all cultural media as compared to control.

• 한국육종학회지
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• 제43권2호
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• pp.126-132
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• 2011

The bulb storability of eight red and ten yellow onion (Allium cepa L.) cultivars grown primarily in Korea was evaluated. During storage, sprouting occurred earlier in red cultivars than in yellow cultivars. In addition, the ratio of sprouted to unsprouted bulbs increased more rapidly in red cultivars than in yellow cultivars. However, not all yellow cultivars had strong storabilities. Bulb storability of three yellow cultivars was as poor as that of red cultivars, suggesting only a slight possibility of a pleiotropic effect of color and bulb storability. Meanwhile, $F_3$ lines of a red cultivar selected based on stronger storabilities showed intermediate storabilities between those of the red and yellow cultivars, implying that strong storability could be obtained by successive selection. In contrast, $F_1$ hybrids from crosses between yellow and red breeding lines showed poor storability compared with the yellow cultivars, indicating that poor storability may be dominant over strong storability. The relationships between color and other traits that potentially affect storability, such as fresh and dry weight, water content, and firmness of bulbs, were evaluated. No significant differences in these traits were observed between the two colors, although the water content of yellow cultivars and the dry weight of red cultivars were slightly higher than their counterparts. Correlation analyses between bulb storability and other traits, including weight, water content, and firmness, also showed no significant correlations. In this study, no correlations were identified between bulb color, storability, and other traits. However, based on these results, red cultivars with stronger storabilities could be developed through successive selection of bulbs with stronger storabilities.

• Journal of Plant Biotechnology
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• 제49권3호
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• pp.213-221
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• 2022

Wild garlic (Allium victorialis var. platyphyllum, AVVP) is a nontimber forest product used as an edible and medicinal vegetable. AVVP is usually propagated form offspring bulbs but it takes a long time to harvest. Using tissue culture technology could overcome this problem. This study investigated the optimal conditions for shoot multiplication, root growth, and plant growth by scooping AVVP bulbs. AVVP bulbs harvested from Ulleung Island, Korea, the main producer of AVVP, were surface-sterilized and used for in vitro propagation. Shoot multiplication was performed by the scooping method. More than five multiple shoots were induced from scooped tissue in Quoirin and Lepoivre (QL) medium containing plant growth regulators (PGRs); the maximum number of multiple shoots were induced from scooped tissue in QL medium containing 0.45 μM thidiazuron (TDZ) after 16 weeks of culture. Roots were induced directly at the base of the shoots in all treatments. In vitro rooting depended on the type of PGRs, and the best root-inducing treatment was QL medium containing 9.84 μM indole-3-butyric acid (IBA). Plants with in vitro roots were transferred to pots containing artificial soil and successfully acclimatized for 4 weeks. The acclimatized plants showed a survival rate of 80% after 20 weeks and gradually promoted growth depending on the acclimatization period. The results of this study will be of great help to AVVP dissemination through sustainable mass propagation.