• KOREAN JOURNAL OF CROP SCIENCE
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• v.58 no.4
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• pp.331-335
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• 2013

Buckwheat sprout is used as vegetable, and also flour for making noodles, and so on. Currently, information about tissue culture in buckwheat is limited and restricted to micro-propagation. We carried out somatic embryogenesis and plant regeneration using hypocotyl segments as explant of the cultivated buckwheat species, Fagopyrum esculentum which differs from existing studies in the growth regulator combinations used. Maximum callus regeneration was induced on MS medium containing 2,4-dichlorophenoxyacetic acid (2,4-D) $2.0mg{\cdot}L^{-1}$, benzyladenine (BA) $1.0mg{\cdot}L^{-1}$ and 3% sucrose. Friable callus was transferred to solidified MS media containing BA ($1.0mg{\cdot}L^{-1}$) with various concentrations of 2,4-dichlorophenoxyacetic acid for the induction of embryogenesis. The optimum concentrations of growth regulators (for regeneration of plantlet) were indole-3-acetic acid ($2.0mg{\cdot}L^{-1}$), Kinetin ($1.0mg{\cdot}L^{-1}$), BA ($1.0mg{\cdot}L^{-1}$). Only 2,4-D did not show any significant effect on callus induction or embryogenesis. Regeneration of embryonic callus varied from 5% to 20%. Whole plants were obtained at high frequencies when the embryogenic calli with somatic embryos and organized shoot primordia were transferred to MS media with 3% sucrose. The main objective of this research was to develop an efficient protocol for plant regeneration for common buckwheat, and to apply in future for genetic transformation.

• Korean Journal of Plant Resources
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• v.22 no.3
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• pp.209-214
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• 2009

To facilitate the introgression of F. esculentum into the traits of F. homotropicum, several accessions of the hybrids between these two species were pollinated with F. esculentum as the recurrent parent. The embryo in vitro rescue was performed to increase the recovery of backcross progenies. The $F_{2}$ generation was more amenable than $F_{1}$ hybrids to produce backcross progenies. The $F_{1}$ hybrids were backcrossed twice with common buckwheat (pin-type F. esculentum) (recurrent backcrossing). Also, alternate backcrosses with common buckwheat and F. homotropicum (congruity backcrossing) were carried out. Pollen tube growth of BC$F_{1}$ ${\times}$ F. esculentum (thrum) and F. homotropicum ${\times}$ BC$F_{1}$ was the disturbed penetration exceeded for all initial interspecific hybrids, and its requirement was proportionally lower when the common buckwheat was used as the recurrent parent and as the last parent of congruity hybrids. Effects of both common buckwheat and F. homotropicum on seed success rate for hybridization were observed. Growth of hybrid embryos before rescue, regeneration of mature hybrids all increased recurrent and congruity backcrosses and inter-crosses between $F_{1}$ plants and selected fertile plants of the second congruity backcrosses.

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2003.10a
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• pp.155-155
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• 2003

• Proceedings of the Korean Society of Crop Science Conference
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• 1995.05a
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• pp.24-25
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• 1995

• Proceedings of the Korean Society of Life Science Conference
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• 2005.04a
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• pp.160-160
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• 2005

• Proceedings of the Korean Society of Crop Science Conference
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• 2019.09a
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• pp.20-20
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• 2019

• The Korean Journal of Food And Nutrition
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• v.7 no.4
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• pp.267-273
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• 1994

To provide the effective application scheme and basic information of buckwheat (Fagopyrum esculentum Moench), buckwheat was germinated at 10$^{\circ}C$ for 7 days and 100 kernels weight, germination rate, root length, chemical composition and mineral contents were examined at 24 hour Intervals. During the germination period, the 100 kernels weight increased approximately 0.3g per day. The germination rate increased sharply after 2 days and the root length increased greatly after 4 days. The crude protein contents increased with germination time, whereas the carbohydrate contents decreased. The crude ash and fat contents did not differ significantly during the germination period. The Ca contents Increased for the 4th day of gemination, but gradually decreased afterwords. The Na contents increased in the initial stage of germination, but then gradually decreased. However, there were no significant change In the Mg, K. Fe. Mn and Zn contents.

• The Korean Journal of Food And Nutrition
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• v.8 no.1
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• pp.32-36
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• 1995

Changes in the w-amylase activity and free sugar contents were Investigated during buckwheat (Fagopyrum esculentum Moench) germination at 1$0^{\circ}C$ for 7 days. The a-amylase activity in ungerminated seeds was 1.66 U. It increased for the 1st day of germination, but then decreased until 3rd day, and thereafter Increased. The free sugar contents In ungerminated seeds were as follows. The maltose, fructose, glucose and rhamnose were 1.81mg%, 0.42mg%, 7.71mg%, 6.80mg% on dry weight basis, respectively. The maltose and fructose contents decreased in the initial stage of germination, but then gradually increased. The glucose contents decreased for the 3rd day, but sharply Increased afterwords. The rhamnose contents decreased until 1 day, and then there was no significant change for the 6 days.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2021.04a
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• pp.26-26
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• 2021

메밀(Fagopyrum esculentum Moench)은 마디풀과(Polygonaceae) 메밀속 (Fagopyrum)으로 약 20종이 있으며, 전 세계 인류 역사와 함께해 왔으며, 오랫동안 사랑을 받아 온 작물이다. 메밀 생태형 분류는 늦게 파종할수록 종실 수량이 떨어지는 여름메밀, 반대로 늦게 파종할수록 종실 수량이 증가하는 가을메밀과 중간형 메밀이 있다. 여름메밀 대표품종은 양절메밀, 양절메밀2호가 있다. '양절메밀'은 춘파·하파 재배가 가능한 여름메밀 품종으로, 1994년 육성되어 장려 품종으로 결정되었다. '양절메밀'의 특성은 유한생육 초형으로, 조숙·단간·다수성 이며, 꽃색은 흰색이며 종자는 흑색이다. 성숙일수는 60-64일로 짧으며, 성숙 후에도 탈립이 강하고, 경장은 단간으로, 도복에 강한 편이다. 그 후 수량성이 증가된 '양절메밀 2호'가 육성되었다. 양절메밀2호는 대립종자로 종자가 흑갈색이며 천립중도 29.5g으로 양절메밀보다 무겁다. 가을메밀로는 대산메밀, 다원 및 순백이 육성되었다. 싹용인 '대산메밀'은 줄기가 연홍색이고 종자가 갈색이었다. '대산메밀'은 가을메밀 품종으로 가을 재배에 적합하다. 경장은 중장간종이며, 생육 일수는 양절메밀보다 약간 늦은 중생종이다. 메밀싹 수율이 높아 새싹 재배에 적합한 것으로 나타났다.

• Journal of the Korean Society of Food Science and Nutrition
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• v.29 no.2
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• pp.280-287
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• 2000

Generally, buckwheat has been regarded as a crop of secondary importance in many countries. In vitro functionalities of buckwheats as a food were evaluated in this study. Five of buckwheat cultivars were extracted with methanol, and the extractant were dried and lyophilized, separately. Or water soluble buckwheat components were digested with the commercial enzymes and the obtained protein hydrolysate was again fractionated by acid precipitation. The antioxidant capacity of the methanol extracts determined using Fe2+-ascorbic acid system was dependent ont the cultivars: The extract of Suwon 4 showed 3.3 times stronger activity than ascorbic acid in terms of IC50. Also, the extracts of buckwheats inhibited efficiently the activities of $\alpha$-amylase and lens aldose reductase. Buckwheat soluble protein or rutin suppressed the in vitro activities of angiotensin-converting enzyme, and the inhibitory degree depended largely on the cultivars. Buckwheat proteins exerted higher hydrophobicity being related to the sterol binding capacity than casein. The results suggested that buckwheat seeds may be desirable and functional food resources in human living in current society.