• Asian Pacific Journal of Cancer Prevention
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• v.16 no.16
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• pp.7129-7138
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• 2015

Background: The polycystic ovary syndrome (PCOS), characterized by hyperandrogenism and chronic anovulation, is a common endocrine disorder in women. PCOS, which is associated with polycystic ovaries, hirsutism, obesity and insulin resistance, is a leading cause of female infertility. In this condition there is an imbalance in female sex hormones. All the sequelae symptoms of PCOS gradually lead to cancer in the course of time. It is heterogeneous disorder of unknown etiology so it is essential to find the exact cause. Materials and Methods: In this study both invasive and non-invasive techniques were employed to establish the etiology. Diagnosis was based on Rotterdam criteria (hyperandrogenism, ovulatory dysfunction, PCOM) and multiparameters using buccal samples and dermatoglypic analysis and cytogenetic study for 10 cases and four age and sex matched controls. Results: In clinical analysis we have observed the mean value of total testosterone level was 23.6nmol/L, total hirsutism score was from 12-24, facial acne was found in in 70% patients with 7-12 subcapsular follicular cysts, each measuring 2-8 mm in diameter. In dermatoglypic analysis we observed increases in mean value ($45.9^{\circ}$) of ATD angle when compared with control group and also found increased frequency (38%) of Ulnar loops on both fingers (UU), (18%) whorls on the right finger and Ulnar loop on left finger (WU) and (16%) arches on right and left fingers (AA) were observed in PCOS patients when compared with control subjects. Features which could be applied as markers for PCOS patients are the presence of Ulnar loops in middle and little fingers of right and left hand. The buccal micronucleus cytome assay in exfoliated buccal cells, we found decrease in frequency of micronuclei and significant increases in frequency of karyolysed nuclei in polycystic ovarian syndrome patients. Chromosome aberration analysis revealed a significant increase in frequency of chromosome aberrations (CAs) in PCOS patients when compared with controls. Conclusions: From this present work it can be concluded that non-invasive technique like dermatoglypics analysis and buccal micronucleus cytome assays with exfoliated buccal cell can also be effective biomarkers for PCOS, along with increased CAs in lymphocytes as a sign of genetic instability. There is a hypothesis that micronuclei and chromosomal aberrations could have a predictive value for cancer. From this present work it can be concluded to some extent that non-invasive technique like dermatoglypics and buccal cell analysis can also be effective for diagnosis.

• Animal Systematics, Evolution and Diversity
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• v.24 no.1
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• pp.81-88
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• 2008

Two oxytrichid ciliates collected from the mosses and estuarine littoral in Korea were identified as Oxytricha longigranulosa Berger and Foissner, 1989 and O. marina Kahl, 1932. These species are reported for the first time from Korea. The description was based on living and protargol impregnated specimens. Diagnostic characters for each species are as follows. Oxytricha longigranulosa: Cell in vivo $80-115{\times}30-50{\mu}m$, mostly $90{\times}40{\mu}m$. Length/width ratio about 2.4/1. Cortical granules about $1{\times}1.5{\mu}m$ in size, colorless, arranged in short and discontinued longitudinal rows. Four frontoventral cirri. Adoral zone of membrane lies (AZM) covering 30-50% of cell length with 25-27 adoral membranelles (AM). Buccal area flat, typical Oxytricha pattern. Five transverse cirri, 19-23 right marginal cirri, 19-24 left marginal cirri, three caudal cirri, five dorsal kineties. Two macronuclear nodules 2 in number and spherical in shape, two micronuclei in number. Oxytricha marina: Cell in vivo $100-150{\times}30-60{\mu}m$. Cytoplasm colorless without cortical granules. Four frontoventral cirri. AZM covering 50% of cell length with 28-44 AMs, Buccal area flat, typical Oxytricha pattern. Five transverse cirri, 23-38 right marginal cirri, 19-25 left marginal cirri, three caudal cirri, five dorsal kineties. Two macronuclear nodules and spherical in shape, 1-5 micronuclei, mostly two in number.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.7
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• pp.3409-3416
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• 2012

Aim: It is well known that polycyclic aromatic hydrocarbons (PAHs) such as benzo (a) pyrene have carcinogenic properties and may cause many types of cancers in human populations. Genetic susceptibility might be due to variation in genes encoding for carcinogen metabolizing enzymes, such as cytochrome P-450 (CYP450). Our study aimed to investigate the effect of genetic polymorphisms of CYP1A1 (m1 and m2) on genetic damage in 115 coal-tar workers exposed to PAHs at their work place. Methods: Genetic polymorphisms of CYP1A1 were determined by the PCR-RFLP method. Comet and buccal micronucleus assays were used to evaluate genetic damage among 115 coal tar workers and 105 control subjects. Results: Both CYP1A1 m1 and CYP1A1 m2 heterozygous and homozygous (wt/mt+mt/mt) variants individually as well as synergistically showed significant association (P<0.05) with genetic damage as measured by tail moment (TM) and buccal micronuclei (BMN) frequencies in control and exposed subjects. Conclusion: In our study we found significant association of CYP1A1 m1 and m2 heterozygous (wt/mt)+homozygous (mt/mt) variants with genetic damage suggesting that these polymorphisms may modulate the effects of PAH exposure in occupational settings.

• Animal Systematics, Evolution and Diversity
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• no.nspc5
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• pp.71-79
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• 2005

Two hypotrichous ciliates inhabiting the representative terrestrial habitats of Jindo Island, Holostichides chardezi Foissner, 1987 and Rigidocortex octonucleatus (Foissner, 1988) are found from Jindo Island. These two species are newly recorded from Korea. The description was based on the observation of living specimens, protargol impregnated specimens and biometric analysis. The morphological variations among the populations of these species were investigated with morphometry. The diagnostic characteristics of these species are as follows: Holostichides chardezi : elongate body flexible and twisted, yellowish subpellicular granules scattered entire cortex, adoral zone of membranelles covering about $28\%$ of body length, left and right rows of mid-ventral cirri paired, left row ended at buccal vertex and right row extending to center of body, no transverse cirri, both marginal rows of cirri not confluent at posterior end, 28-42 macronuclei and 1-5 micronuclei scattered entire body. 3 caudal cirri and 4 dorsal kineties. Rigidocortex octonucleatues: elongate-oval body rigid and not contractile, yellowish cytoplasmic crystals and fat globules densely packed, adoral zone of membranelles covering about $40\%$ of body length, 5 transverse cirri, both marginal rows of cirri not confluent at posterior end, 8 macronuclei longitudinally arranged in median of cell, 3 caudal cirri and 7-10 fragmented dorsal kineties.