• Journal of Plant Biotechnology
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• v.29 no.3
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• pp.151-159
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• 2002

Genetic map and molecular marker have a great importance in improving and facilitating crop breeding program as well as in genome analysis and map-based cloning of genes representing desirable characters. This study aimed at developing RAPD markers and constructing a genetic linkage map using 82 BC$_1$F$_1$individuals originated from the cross between '835' and B$_2$in radish (Raphanus sativus L.). One of the parents for genetic linkage map construction, '835'(P$_1$) of egg type is susceptible to Fusarium wilt and have medium resistance to virus infection and the other parent, B$_2$(P$_2$) of round type, is susceptible to Fusarium wilt and virus, Screening of 394 RAPD primers in BC$_1$F$_1$) population resulted in selecting 128 polymorphic markers which displayed 1:1 segregation pattern. Two markers failed to display 1:1 segregation and showed the segregation ratio skewed to maternal genotype. Selected markers were categorized into 14 linkage group based on LOD score represented by MAPMAKER/EXP program. Five groups composed of single marker among them were excluded from the linkage map, and consequently, the remaining groups are well matched with the number of radish chromosome (n＝9). The linkage map constructed with 128 markers covers 1,688.3 cM and the average distance between markers was 13.8 cM. For developing STS marker, we determined the partial nucleotide sequence of OPE10 marker at both ends and designed a oligonucleotide primer pair based on this sequence. STS PCR using the primer pair displayed a single, clear band of which segregation is perfectly matched with that of OPE10 marker. This implies that RAPD markers could readily convert into clear and reliable STS markers.

• Journal of Life Science
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• v.31 no.7
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• pp.626-630
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• 2021

This study was conducted to examine the association between the myosin heavy chain 3 (MYH3) in 6-bp deletion variant genotypes and meat color traits in a crossbred pig population Landrace and Jeju native black pigs (JNBP). The longissimus dorsi, semimembranosus, triceps brachii and biceps femoris muscle from each carcass were used for the analysis of meat color traits. A total of 187 pigs and three meat color traits, CIE L^* (lightness), CIE a^* (redness), and CIE b^* (yellowness), were analyzed. All experimental pigs were successfully genotyped for the MYH3 6-bp deletion variant using Polymerase chain reaction (PCR) analysis. We detected three MYH3 6-bp deletion variant genotypes qq, Qq, and QQ with 0.091, 0.551 and 0.358 genotype frequencies, respectively. Compared to qq homozygotes, the MYH3 6-bp deletion QQ genotype animals showed a higher levels of the meat colors traits CIE L^* (lightness), CIE a^* (redness), and CIE b^* (yellowness) in longissimus dorsi (p>0.05, p<0.001, p<0.001), semimembranosus (p>0.05, p<0.001, p<0.001), triceps brachii (p<0.001, p<0.001, p<0.001), and biceps femoris (p<0.01, p<0.001, p<0.001), respectively. The QQ genotype pigs was associated with increasing meat color traits in the crossbred between Landrace and JNBP. Our findings suggest that the MYH3 6-bp deletion variant genotypes can be used as valuable genetic markers for JNBP-related breeding programs to improve meat quality and control meat color traits.

• The Korean Journal of Zoology
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• v.14 no.2
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• pp.57-74
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• 1971

The present investigation has been done to observe the ecological habits of field mice to protect the rice from damages during the growing season in paddy-field and during the storge period. The results obtained are summarized as follows: 1. Of 155 mice captured in the period of April-November 1970, which belong to four genera (Apodemus, Cricetulus, Rattus, and Micromys), 148 mice(95%) were found as striped field mice (Apodemus agrarius coreae). The population density of striped field mouse was revealed by the present study as 55/ha, which is quite a low level compared with that in Japan of 900/ha. 2. The age distribution of the mice as judged by their body weight was found mainly composed of adult and the sex ratio was found to be 1.8 as determined with 147 individuals. The nest was found to be occupied by an adult and was composed of at least three openings and more than one food storage tunnels. The mice usually keep hulled rice rather than unhulled one in storage tunnel. The weight of food found in a nest was about 50 grams on an average. 3. The mice show a most active behaviour 1-2 hours after the sunset, around midnight, and an hour before the sunrise, but they are active even in daytime in order for searching for food and for breeding. 4. The ratio (%) of damage appeared in high stem of sweet corn in August was 30 ~ 40 percent, whereas that in low stem was 80 ~ 90 percent. The weight of spoiled grains in paddy-field was 11, 400gm/0.4ha and this gives an estimate of 349, 695 for whole country. 5. The female striped field mouse weighs average of about 30 grams and gives birth to average of 4.8 younglings which wean away from female mouse three weeks after delivery. 6. The natural enemies to the mice are found to be carnivores (weasel, cat, mountain cat, fox, raccoon, and otter), raptatores(eagle, owl, kete, buzzard), and snakes. Two kinds of field rats(Rattus norvegicus, Cricetulus tritor) are also the predator to the mice. 7. The feeding preference of striped field mice follows in decreasing order of sweet corn, soybean, sweet potatoes, chestnut, and wheat. The mice do not have a preference for barley, millet, rough millet, red bean, and green bean. 8. The starvation experiment, in which water alone was supplied, revealed that the mice in good physical and nutritional conditions survived for 71 ~ 79 hours, whereas those in worse conditions survived for only 32 ~ 39 hours.