• Title/Summary/Keyword: breeding data

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APPLICATION OF RANDOMLY AMPLIFIED POLYMORPHIC DNA(RAPD) ANALYSIS METHOD FOR CLASSIFICATION AND BREEDING OF THE KOREAN GINSENG

  • Lim Y.P.;Shin C.S.;Lee S.J.;Youn Y.N.;Jo J.S.
    • Proceedings of the Ginseng society Conference
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    • 1993.09a
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    • pp.138-142
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    • 1993
  • Korean ginseng has been widely used as medicine from ancient times in Asia. Current breeding efforts in Korea include the individual plant selection and the subsequent pure - line isolation, and considerable number of lines with desirable traits have thus been isolated. However, there were rare data on genetic maker and its analysis for selection of superior varieties. For taxonomic characterization and development of genetic markers for ginseng breeding, molecular biological methods including the RFLP and RAPD methods were applied. Cytoplasmic DNA of ginseng was analyzed for RFLP analysis. However. there is no different pattern among the chloroplast DNA or mitochondrial DNA of variants. In the case of RAPD analysis, the band patterns using 4 of 10 RAPD primers show the distinctive polymorphism among 9 ginseng variants, and lines, and Similarity Index(SI) on polymorphism was calculated for the extent and nature of these variabilities in ginseng. The sequences of 4 selected primers were TGCCGAGCTG, AATCGGGCTG. GAAACGGGTG, and GTGACGTAGG. By SI based on the polymorphic band patterns, Chungkyung - Chong and Hwangskoog - Chong, and JakyungChong 81783 and Jinjakyung of Russia showed the most close SI. The data of KG10l coincided with the fact that it was released from Hwangskoog - Chong. and Jakyung - Chong 81783 and Jinjakyung of Russia showed the most close SI. The data of KG101 coincided with the fact that it was released from Hwangskoog - Chong by breeding process. The data of Jakyung strains indicated the significant variation among the strains. From these results, RAPD analysis method could be succesively applied to the classification and genetic analysis for breeding of Korean ginseng.

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Program Development of Genetic Analysis for Diallel Cross Experiment

  • Kim, Seo Young;Bae, Jong Sung
    • Communications for Statistical Applications and Methods
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    • v.9 no.3
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    • pp.675-682
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    • 2002
  • In this study, we develop the statistical analysis program for genetic analysis of diallel crosses data by SAS/MACRO, SAS/IML. Genetic analysis is to estimate of genetics parameters and heredity with reciprocal cross and without reciprocal cross. Statistical analysis program solve the problem of the difficulties on the data analysis in field denetics and breeding Therefore the user whoever want to analysis of data on genetics and breeding easily conduct the work saving time and suffering.

Efficacy of Auxiliary Traits in Estimation of Breeding Value of Sires for Milk Production

  • Sahana, G.;Gurnani, M.
    • Asian-Australasian Journal of Animal Sciences
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    • v.12 no.4
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    • pp.511-514
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    • 1999
  • Data pertaining to 1111 first lactation performance record of Karan Fries (Holstein-Friesian $\times$ Zebu) cows spread over a period of 21 years and sired by 72 bulls were used to examine the efficiency of sire indices for lactation milk production using auxiliary traits. First lactation length, first service period, first calving interval, first dry period and age at first calving were considered as auxiliary traits. The efficiency of this method was compared with simple daughter average index (D), contemporary comparison method (CC), least-square method (LSQ), simplified regressed least-squares method (SRLS) and best linear unbiased prediction (BLUP) for lactation milk production. The relative efficiency of sire evaluation methods using one auxiliary trait was lower (24.2-32.8%) in comparison to CC method, the most efficient method observed in this study. Use of two auxiliary traits at a time did not further improve the efficiency. The auxiliary sire indices discriminate better among bulls as the range of breeding values were higher in these methods in comparison to conventional sire evaluation methods. The rank correlation between breeding values estimated using auxiliary traits were high (0.77-0.78) with CC method. The rank correlation among auxiliary sire indices ranged from 0.98 to 0.99, indicating similar ranking of sire for breeding values of milk production in all the auxiliary sire indices.

First Report of Annual Habitat Ranges in Black-tailed Gulls (Larus crassirostris) Breeding on Dokdo Island (독도에서 번식하는 괭이갈매기의 연간 서식 범위 첫 보고)

  • Hong, Mijin;Kim, Miran;Lee, Ho;Cho, Sang-moon
    • Ocean and Polar Research
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    • v.41 no.2
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    • pp.99-105
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    • 2019
  • Black-tailed gulls, Larus crassirostris, have been known as resident birds in Korea, but their wintering and breeding habitat ranges were little known about. We investigated the habitat ranges of Blacktailed gulls breeding on Dokdo Island - which is the eastern end of their breeding colonies in Korea. Three adult Black-tailed gulls were fitted with Global Positioning System data loggers in May 2018 and their positions were tracked for 9 days, 245 days and 365 days each respectively. Black-tailed gulls stayed on Dokdo Island until June for breeding purposes and moved down to the southern part of Japan for wintering. The following year, a Black-tailed gull started to move northward in February and returned to Dokdo Island in April. They traveled a total of $207,334-229,507km^2$ (MCP) throughout a whole year. Based on location density categories accounting for 50% of locations, they used 3,618 to $3,803km^2$ in area. Black-tailed gulls breeding on Dokdo Island used smaller habitat ranges during the wintering period than the other periods. This is the first report on the habitat ranges of Black-tailed gulls breeding on Dokdo Island.

Bridging Comparative Genomics and DNA Marker-aided Molecular Breeding

  • Choi, Hong-Kyu;Cook, Douglas R.
    • Korean Journal of Breeding Science
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    • v.43 no.2
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    • pp.103-114
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    • 2011
  • In recent years, genomic resources and information have accumulated at an ever increasing pace, in many plant species, through whole genome sequencing, large scale analysis of transcriptomes, DNA markers and functional studies of individual genes. Well-characterized species within key plant taxa, co-called "model systems", have played a pivotal role in nucleating the accumulation of genomic information and databases, thereby providing the basis for comparative genomic studies. In addition, recent advances to "Next Generation" sequencing technologies have propelled a new wave of genomics, enabling rapid, low cost analysis of numerous genomes, and the accumulation of genetic diversity data for large numbers of accessions within individual species. The resulting wealth of genomic information provides an opportunity to discern evolutionary processes that have impacted genome structure and the function of genes, using the tools of comparative analysis. Comparative genomics provides a platform to translate information from model species to crops, and to relate knowledge of genome function among crop species. Ultimately, the resulting knowledge will accelerate the development of more efficient breeding strategies through the identification of trait-associated orthologous genes and next generation functional gene-based markers.

HCCR breeding blankets optimization by changing neutronic constrictions

  • Zadfathollah Seighalani, R.;Sedaghatizade, M.;Sadeghi, H.
    • Nuclear Engineering and Technology
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    • v.53 no.8
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    • pp.2564-2569
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    • 2021
  • The neutronic analysis of Helium Cooled Ceramic Reflector (HCCR) breeding blankets has been performed using the 3D Monte Carlo code MCNPX and ENDF nuclear data library. This study aims to reduce 6Li percentage in the breeder zones as much as possible ensuring tritium self-sufficiency. This work is devoted to investigating the effect of 6Li percentage on the HCCR breeding blanket's neutronic parameters, such as neutron flux and spectrum, Tritium Breeding Ratio (TBR), nuclear power density, and energy multiplication factor. In the ceramic breeders at the saturated thickness, increasing the enrichment of 6Li reduces its share in the tritium production. Therefore, ceramic breeders typically use lower enriched Li from 30% to 60%. The investigation of neutronic analysis in the suggested geometry shows that using 60% 6Li in Li2TiO3 can yield acceptable TBR and energy deposition results, which would be economically feasible.

Folic acid supplementation regulates key immunity-associated genes and pathways during the periparturient period in dairy cows

  • Khan, Muhammad Zahoor;Zhang, Zhichao;Liu, Lei;Wang, Di;Mi, Siyuan;Liu, Xueqin;Liu, Gang;Guo, Gang;Li, Xizhi;Wang, Yachun;Yu, Ying
    • Asian-Australasian Journal of Animal Sciences
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    • v.33 no.9
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    • pp.1507-1519
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    • 2020
  • Objective: The current research was aimed to profile the transcriptomic picture of the peripheral blood lymphocytes (PBLs) associated with immunity in Chinese Holsteins supplemented orally with coated folic acid during the periparturient period. Methods: The total of 123 perinatal cows were selected for this study and divided into three groups; group A (n = 41, 240 mg/500 kg cow/d), group B (n = 40, 120 mg/500 kg cow/d) and group C (n = 42, 0 mg/cow/d) based on the quantity of folic acid fed. Three samples of PBLs were selected from each folic acid treated group (high, low, and control) and RNA sequencing method was carried out for transcriptomic analysis. Results: The analysis revealed that a higher number of genes and pathways were regulated in response to high and low folic acid supplementation compared to the controls. We reported the novel pathways tumor necrosis factor (TNF) signaling, antigen processing and presentation, Staphylococcus aureus infection and nuclear factor (NF)-kappa B signaling pathways) and the key genes (e.g. C-X-C motif chemokine ligand 10, TNF receptor superfamily member 1A, cluster difference 4, major histocompatibility complex, class II, DQ beta, NF-kappa-B inhibitor alpha, and TNF superfamily 13) having great importance in immunity and anti-inflammation in the periparturient cows in response to coated folic acid treatment. Conclusion: Collectively, our study profiled first-time transcriptomic analysis of bovine lymphocytes and compared the involved cytokines, genes, and pathways between high vs control and low vs control. Our data suggest that the low folic acid supplementation (120 mg/500 kg) could be a good choice to boost appropriate immunity and anti-inflammation as well as might being applied to the health improvement of perinatal dairy cows.

Genetic Parameters and Annual Trends for Birth and Weaning Weights of a Northeastern Thai Indigenous Cattle Line

  • Intaratham, W.;Koonawootrittriron, S.;Sopannarath, P.;Graser, H.-U.;Tumwasorn, S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.21 no.4
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    • pp.478-483
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    • 2008
  • Records of a Northeastern Thai indigenous cattle line population were used to estimate genetic parameters and annual trends for calf weights. The data set comprised records of 1,922 and 1,489 animals for birth and weaning weight, respectively born from 1993 to 2004. A bivariate analysis was carried out for variance and covariance components estimations using average information restricted maximum likelihood procedure. Average estimated breeding value and maternal breeding value of the animals born in 1993 were set to zero as a base group. Genetic trends of each trait were calculated by regressing average estimated breeding values and maternal breeding values on birth year of calves. Phenotypic trends for each trait were calculated by regressing the yearly adjusted weight on birth year of calves. The results revealed that the estimate of direct heritability, maternal heritability and maternal permanent environmental variance as a proportion of phenotypic variance for birth and weaning weight was 0.40, 0.14 and 0.04; 0.27, 0.05 and 0.23, respectively. Direct heritability was moderately heritable and genetic improvement through selection can be achieved. The estimate of phenotypic, direct genetic, maternal genetic and maternal permanent environmental correlation between birth and weaning weight was 0.48, 0.65, 0.98 and 0.73, respectively. The phenotypic trend, genetic trends of estimated breeding value and maternal breeding value for birth weight was 0.18, 0.04 and 0.01 kg/year, respectively. The phenotypic trend, genetic trends of estimated breeding value and maternal breeding value for weaning weight was -1.36, 0.32 and 0.03 kg/year, respectively. As maternal genetic effect was considerably less important than direct genetic effect, selection for improved weaning weight of this Northeastern Thai indigenous cattle line can place more emphasis on the direct genetic effect.

Pedigree Analysis of 17 High Quality Korean Rice Cultivars Using Web Database Systems

  • Yi, Gi-Hwan;Park, Dong-Soo;Chung, Eun-Sook;Song, Song-Yi;Jeon, Nam-Soo;Nam, Min-Hee;Kim, Doh-Hoon;Han, Chang-Deok;Eun, Moo-Young;Ku, Yeon-Chung
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.51 no.6
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    • pp.554-564
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    • 2006
  • It is important to understand pedigree of rice cultivars commonly used for breeding. In this paper, pedigree tables for tracking the pedigree of 17 representative rice cultivars recommended by Rural development Adminstration (RDA) were completed and analyzed using two kinds of web database system; 'IRIS' and 'RRDB'. Seven cultivars, namely, 'Sangmibyeo', 'Ilpumbyeo', 'Saegewhabyeo', 'Surabyeo', 'Shindongjinbyeo', 'Ilmibyeo' and 'Jungwhabyeo' had 'Koshihikari' on the pedigree of their ancestor. Besides 'Koshihikari', the most feguently used ancestral germplasms among the high quality rice cultivars were 'Fujisaka 5', 'Kameno o' and 'Asahi', 'Fujisaka 5' was used as ancestral parent in 12 out of 17 cultivars. Interestingly, 'Kameno o' was used in pedigree of 16 out of 17 high quality varieties and 'Asahi' was used in the ancestral pedigree of all 17 varieties. 'Hwayeongbyeo' was used as one of parent in the breeding of 'Dongjin 1', 'Hwabongbyeo', 'Saegewhabyeo' and 'Junambyeo'. 'Ilpumbyeo' was used in the breeding pathway of 'Junambyeo' and 'Saegewhabyeo', 'Mangeumbyeo' itself was not enlisted as one of high quality rice cultivars, but was used as a breeding parent of three high quality varieties, namely, 'Saegewhabyeo', 'Hwabongbyeo' and 'Nampyeongbyeo'. Incorporated with evaluation data, pedigree will provide a valuable chance to genealogical tracking of agronomic traits such as disease resistance, grain quality and etc.

Estimation of Genetic Parameter for Growth Traits of Olive Flounder Paralichthys olivaceus on the 8th Generation of Selective Breeding Using Multiple Traits Animal Model (다형질 Animal Model을 이용한 선발 8세대 육종 넙치(Paralichthys olivaceus)의 성장형질에 대한 유전모수 추정)

  • Park, Jong-Won;Lee, Dain;Jung, Hyo Sun;Kim, Julan;Yang, Hye-Rim;Kim, Hyun-Chul;Lee, Jeong-Ho
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.55 no.5
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    • pp.549-556
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    • 2022
  • The aim of this study was to evaluate the genetic parameters of growth traits for improvement breeding in olive flounder Paralichthys olivaceus at the 8th generation of selective breeding in April 2021. Growth traits such as total length, body weight and condition factor at 11 months of age were measured for 7,508 individuals with confirmed paternity. Data were analyzed using the restricted maximum likelihood method applied to a multiple traits animal model. The effects of sex and family were significantly different across traits (P<0.05). The heritability values of total length, body weight and condition factor were estimated to be high as 0.479, 0.457, and 0.466, respectively. Correlation analysis between phenotypic and breeding values, indicated that the selection accuracy was 75.9-85.2% for all traits. To increase the selection accuracy for parent fish selection, the sex and pedigree characteristics that affect each trait should be considered. Moreover, further improvement of multiple traits can be achieved if the correlation between each trait is appropriately considered.