• KOREAN JOURNAL OF CROP SCIENCE
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• v.34 no.4
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• pp.370-377
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• 1989

Jerusalem artichoke (Helianthus tuberosus L.) as a biomass potential crop has several distinct advantages such as vigorous growth on poor land and high yield of carbohydrate. In this crop, seed dormancy has hampered the efforts of seed-propagration and to use them in breeding programs for improving jerusalem artichoke. Several seed treatments were tested to determine their effectiveness in overcoming the seed dormancy found in five collected varietes of jerusalem artichoke. The first results showed that the seed fertilities of five collected varieties ranged from 2.4％ to 14.7％ and the number of seed produced by one plant ranged from 88 to 1058. Germinability of seeds stored for 3 months at room temperature after harvest was almost 0％ and it was not improved by addition to the treatments of temperature, light and GA3, while germinability of seeds stored for 27 months at room temperature after harvest increased to 47.5％ in germination rate. But the removal and pin-pricking of seedcoat were very effective in breaking the seed dormancy, giving germination of 96.8％ and 82.3％, respectively. These results showed that the seed dormancy of jerusalem artichoke was induced by the seedcoat. Besides the treatment of seedcoat removal and seedcoat-pinpricking, the treatment of low and wet stratification was also effective in breaking the seed dormancy of jerusalem artichoke. Whole dormant seeds incubated for 70 days in low and wet condition germinated over 85％.

• Horticultural Science & Technology
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• v.29 no.6
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• pp.651-654
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• 2011

Cymbidium 'Orange Bowl' (Lucky Rainbow 'Randevous' ${\times}$ 'Eastern Star') was developed from a cross between hybrids at the National Institute of Horticultural & Herbal Science, Rural Development Administration in 2006. A cross was made between the pink colored flower C. Lucky Rainbow 'Randevous' as maternal line and pure yellow colored flower, C. 'Eastern Star' as paternal line in 1995. The seed germination, cultivation, selection, and characteristic trials were conducted from 1996 to 2006. The line was named as Wongyo F1-18 and phenotype was characterized in 2006 as a new 'Orange Bowl'. The 'Orange Bowl' has having light yellow basal color (RHS, YO21D) and orange line (RHS, OR30B) on both of sepal and petal with red lip (RHS, OR30B). 'Orange Bowl' has about 10.9 flowers per flower stalk and flower size of 7.4 cm. General appearance of the petals and sepals is slightly incurved shape. The plant size is intermediate having erect peduncle. Blooming is started from the late of January (mid winter) under optimal culture condition. Leaf attitude and twisting is half- erect and very weak respectively. This hybrid has attractive floral arrangement, long flower stalk (71.8 cm) and vigorous growth. We expect that 'Orange bowl' has a great potential for exporting to Chinese market.

• Journal of the Korean Society for Marine Environment & Energy
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• v.4 no.2
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• pp.43-51
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• 2001

Now our ocean environment pollution is very serious. Its harm hinders in marine breeding and the safe navigation of ships at the coast. We have used an assembly system for a measure taken against environment pollution like this. But, here are some problems awaiting solution. First, most of combustible materials among ocean waste are high polymer, so it is necessary some special equipment to incinerate them. In the process we can't overlook air pollution by exhaust gas. Also, when we reclaim these wastes, we remember that they can't be decomposed naturally and leaking water may pollute soil. Thus now a days new treatment method has been developed, it recycles and doesn't product secondary pollution materials by recovering oil from pyrolysis. For it, this study investigated chemicalㆍphysical properties of wastes. And it found condition of recovering the most oil. Also it probed that the variation of temperature raising speed affects the weight reduction characteristics of wastes. Also, while studying recovered oil by waste pyrolysis and the rate of non-condensing gas in accordance with the variation of temperature raising speed. Finally we had confidence the development of pyrolysis oil recovery would succeed because we carried out evaluation at an economic point of view about it.

• Journal of Sericultural and Entomological Science
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• v.47 no.2
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• pp.93-98
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• 2005

The antioxidant capacity of yield-type mulberry leaves and fruits (Morus alba L., M. bombycis Koidz, and M. Lhou (Ser). Koidz) collected from In-je, Won-ju and Yang-yang regions, Kang-won province, Korea, was investigated with minium L-100 device and ARAW-KIT (anti-radical ability of water-soluble substance), in comparison to the ascorbic acid. The results indicated that total antioxidant capacity of yield-type mulberry leaves was 2711.2 nmol (ascorbic acid equivalents). The highest stage of antioxidant capacity (3587.6 nmol) was opening stage of five leaves in spring. Especially, antioxidant capacity of mulberry leave collected from In-je region was high than that of other regions. No. 7 mulberry leave showed the strongest antioxidant capacity (6184.9 nmol) at opening stage of five leaves in spring. Also, fluit growing condition including fruit size and yield was good, too. Therefore, No. 7 mulberry three from In-je region, possessed not only high antioxidant capacity but also considerable fruit quality, was selected as potent resource for mulberry breeding and functional material development.

• The Korean Journal of Ecology
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• v.24 no.2
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• pp.93-100
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• 2001

One-year-old cottonwood (Populus deltoides Bartr.) clones, which were classified as sensitive or tolerant, were exposed to 150 n1/1 ozone (O$_3$) over 8 days for 8 hours each day under glass chamber conditions with natural sunlight. The leaves of the sensitive clone had black stipple and bifacial necrosis after $O_3$ treatment. Photosynthesis and stomatal conductance were measured before, during, and after the $O_3$ treatment. The photosynthetic rates due to $O_3$ treatment were decreased 51 percent and 34 percent on the sensitive and tolerant clone, respectively. The stomatal conductance of the sensitive clone was more than 40 percent higher than that of the tolerant clone regardless of the $O_3$ treatment. As light intensity increased, the $O_3$ effect on photosynthesis was clear. Compared to the previous growth chamber studies, our natural light exposure system was able to maintain a stable photosynthetic responses of the control treatment throughout the fumigation period. In addition, changes in assimilation versus intercellular $CO_2$ concentration (A/C curves) showed that $O_3$ decreased the slope and asymptote of the curves for the sensitive clone. This indicates that $O_3$ decreases the biochemical capacity of photosynthesis on the sensitive clone. Chlorophyll contents and fluorescence of the two clones were analyzed to examine the $O_3$ effects on photosystem 11, but $O_3$ did not impact these variables on either clone. Although the tolerant clone did not show any foliar injury, we could not find any ecophysiological defensive responses to $O_3$ treated. Stomatal conductance of the tolerant clone was originally much lower than that of the sensitive one. Thus, the mechanisms of the tolerant clone in this system are to narrowly open stomata and efficiently maintain photosynthesis with a more durable biochemical apparatus of photosynthesis under $O_3$ stress. The sensitive clone has higher photosynthetic capacity and more efficient light reaction activity than the tolerant one under charcoal filtered condition, but is not as resilient under stress.

• Journal of Plant Biotechnology
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• v.34 no.1
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• pp.11-17
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• 2007

Previously developed transgenic watermelon rootstocks (gongdae) inserted by CGMMV-CP were examined to test the virus tolerance levels. In the restricted plastic house, the $T_{3}$ watermelon rootstock showed tolerance to CGMMV until 70 days after inoculation on the leaves while the non-transformed watermelon rootstock became susceptible at 20 days after inoculation. In the field, tolerance efficiency of transgenic rootstocks maintained up to 40% at 71 days after contamination with CGMMV in the soil while all of the non-transformed rootstocks became susceptible at 37 days with the same condition. In the same field, transgenic rootstocks showed more tolerance to CGMMV than the non-transformed rootstocks as those were inoculated on the leaves, but it showed only 10 days delay before being susceptible. Therefore, transgenic rootstocks have a characteristic of delay effect against CGMMV susceptibility, rather than resistance character. From $T_{3}$ rootstocks homozygous for the CGMMV-CP horticulturally favorable individuals were selected for further breeding and a transgenic line was finally obtained at the $BC_{1}T_{5}$. A material transfer experiment was conducted to find out if the DNA, RNA or expressed protein in the transgenic rootstocks could move to the grafted scion (non-transformed watermelon, Super-Kumcheon). PCR, northern, and western blot analysis were performed and no evidence of transferring of those materials from rootstock to scion was ever found.

• Korean Journal of Plant Resources
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• v.31 no.5
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• pp.531-537
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• 2018

This study was carried to survey distribution of the nucleotide polymorphisms in heme-binding (HB) domain, which is highly conserved region between 1,210 and 1,240 bp of cytochrome P450, in domestic garlic cultivars. 120 garlic cultivars collected from Korea were classified into seven HB domain variation based on the nucleotide sequence of the domain. Northern type garlic cultivars, collected from Kyungpook, Chungnam, Chungpook and Kangwon province, showed 51.3% of KP2 type nucleotide sequence, 5'-TTT/GGC/GGT/GGA/CGG/AGA/ATA/TGT/CCT/GGA-3' with coding amino acid FGGGRRICPG, 13.7% of KP1, 11.3% of CP, 8.8% of CM and 5% of KW2 types. Southern type cultivars, collected from Kyungnam province, showed 52.5% of KM type nucleotide sequence, 5'-TTT/GGC/GCA/GGA/CGG/AGA/ATT/TGT/CCT/GGA-3' with coding amino acid FGAGRRICPG, 22.5% of KP2, 5.0% of KW2 and 2,5% of CP type nucleotide sequence. These results showed that Korean garlics were cultivated in highly mixed condition even in the same region.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.55 no.4
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• pp.292-298
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• 2010

This study aims to understand the contribution rate of plant parts on biomass in five winter forage crops including barley, wheat, oat, rye and triticale. In barley and oat, the major plant parts on dry matter yields were changed from culm to spike at 20 days after heading(DAH), but culm was very important part on biomass of rye, wheat and triticales until 30DAH. Wheat, rye and triticales showed same pattern in dry weight increase indicating that culm was main component of biomass from heading stage but spike became heavier after 30 DAR. Barley and oat were similar in dry weight increase where spike was the main components of biomass at 20 DAR. After heading stage the major biomass of most winter forage crops were dependent on the weight of culm and spike, while the contribution of leaves to plant biomass was lower as compared to culm and spike. Thus culm and spike were very important factors for improving the dry matter production of winter forage crops breeding and early maturing is also additively required under Korean climatic condition.

• Research in Plant Disease
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• v.21 no.3
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• pp.243-249
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• 2015

Soybean frogeye leaf spot caused by the fungus Cercospora sojina Hara, has known to lead a severe reduction of crop yield. Since frogeye leaf spot on soybean has recently become a serious problem in Korea, the susceptibility of recent recommended cultivars against C. sojina had been tested. To standardize the disease severity of soybean, the optimum sporulation condition of C. sojina and the disease index were established in this study. Sporulation was maximized on the 10% V8 juice agar with 12 h light and 12 h dark at $25^{\circ}C$. Spore suspension ($10^5spores/ml$) was sprayed on the leaves of soybean (V6 stage), and the disease responses to each isolate were evaluated on 28 days after inoculation. As a result, Daepung, Shinpaldal2ho, Yeonpung and Cheonga showed the resistance reaction to 8, 7, 6, 6 isolates of C. sojina, respectively, whereas Cheongja, Hwangkeum, Taekwang, Daewon, Cheonsang and Sinhwa showed the susceptible reaction to 8 isolates of C. sojina. Breeding the resistant soybean cultivars against C. sojina requires a uniform resistance for screening technique. The disease index of frogeye leaf spot on soybean developed in this study can be effectively used for the accurate field assay to select the frogeye leaf spot resistant soybean.

• Journal of Sericultural and Entomological Science
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• v.5
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• pp.25-38
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• 1965

I. Breeding of tussah silkworm(preliminary report). The preliminary examination for bleeding has been carried out since 1963 in tussah silkworms. 1) The strain(l-MG-B)of the heaviest silk quantity was the green silkworm and brown cocoon in univoltine, and the strains(2-G-B, 2-MG-B) of the heaviest silk quantity were also the green silkwom and brown cocoon in bivoltine in both spring and fall in 1965. 2) It looks like the voltinism, the body color and the cocoon color have reached to pure line up to 1965. II. Best place for the winter of tussah pupa. This work was aimed to find out good ways for the winter of tussah pupa. 1) The hatch of bivoltine was better than that of univoltine. 2) The cocoons covered with the leaves were good in the emergence of moth. 3) The cocoons which were kept at natural temperature till the first emergence of moths would show bad in both hatch and emergence. 4) If some of the pupae kept under natural condition were controled at proper temperature for a few days, hatch and laying eggs were best. 5) The best places for the winter were the egg storage and the rearing room. III. Relation between incubation temperature and voltinism. 1) When the tussah pupa are kept at natural temperature during winter, the moths do not come out of the pupa. 2) There is no difference between about 18$^{\circ}C$ and about 25$^{\circ}C$ during incubation in hatching ratio. 3) The tussah silkworms of univoltine in mortality are stronger than that of bivoltine. 4) There is not any relation between voltinism and high or low temperature for pupa and eggs. IV. Induced mutation by gamma-ray and neutron in tussah silkworm. This work was carried out in order to induce the mutation by treating the pupa or the eggs of tussah silkworm with gamma my and neutron. The results obtained are as follows. 1. Though the whole pupa treated with neutron become moths, the moths have no ability to copulate each other. The only moths emerged from pupa treated with neutron, 4000${\gamma}$ are able to lay all un-fertilized eggs, some of which have a hole on the surface and nothing of contents. 2. The non-diapause eggs are treated with neutron in spring, but the hatching ratio is 50∼60 percent, but the whole eggs treated with gamma ray are never hatched. 3. The sensitivity of the pupa to neutron is weaker than that of the eggs. 4. The hatching ratio is in direct proportion to the gamma ray dose. 5. Author find out a new mutant which is excellent in the cocoon quality, so he will do the progeny test next hear.