Thirty-eight isolates of Phytophthora sp. caused rots on roots and basal stems were collected from five flowering plants from 1992 to 1997 at eight cultivation areas in Korea. All the isolates were identified as P. nicotianae based on following characteristics. The fungus produced markedly papillate, not caducous and ovoid to spherical sporangia, abundant chlamydospores, and small oospores with amphigynous antheridia only when paired with either A1 or A2 mating type. All isolates grew well at 35$^{\circ}C$ and showed distinct arachnoid colony patterns on CMA and PDA. Sizes of sporangia and chlamydospores of five representative isolates from each plant averaged 43-52$\times$30-38 ${\mu}{\textrm}{m}$ and 28 ~34 ${\mu}{\textrm}{m}$. Mating type of the isolates was either A1 or A2, and oogonia and oospores were measured as 28~31 ${\mu}{\textrm}{m}$ and 21~25 ${\mu}{\textrm}{m}$. PCR-RFLP analysis of rDNA of the five isolates resulted that restriction band patterns of the small subunit and ITS regions were identical to a perilla isolate of P. nicotianae, but distinct from P. cactorum and P. capsici. Cross inoculation tests showed that the five isolates had pathogenicity to lily, christmas cactus, anthurium, baby's breath and carnation with different degrees. However, each isolate showed stronger pathogenicity to its corresponding original host than others. Among five lily cultivars Georgia and Quririna were more susceptible than Napoli and others. This is first report of Phytophthora root and stem rot of lily, Christmas cactus, anthurium, baby's breath and monochoria in Korea.
Objectives: This study was performed to produce data on the pulmonary toxicity of neodymium oxide($Nd_2O_3$) by intratracheal instillation. Methods: Two groups of rats were exposed to neodymium oxide by intratracheal instillation with doses of 0.5 mg and 2.0 mg, respectively. At two days, four weeks and 12 weeks after exposure, body weight change, organ weight change and histopathological change were observed. At 12 weeks after exposure, lung function change was measured. Results: The body weight of rats in the high concentration group decreased after 12 weeks by 4-5% compared with the control group. At four weeks and 12 weeks after the administration of neodymium oxide, the absolute weight of the lungs of the high concentration group were significantly increased when compared with the control group(p<0.05). At 12 weeks after the injection of neodymium oxide, breath frequency and respiratory minute volume were increased, but inhalation time and expiratory time were decreased. Bronchiolar epithelial hyperplasia, alveolar type II cell hypertrophy/hyperplasia and foreign body granulomatous inflammation were observed in the high exposure group. Conclusions: Body weight decrease, lung absolute weight and breath frequency increase, and pathological lung change were all observed. We found that pulmonary toxicity of neodymium oxide nanoparticles by intratracheal instillation could be confirmed.
To select the criteria, literature review was made in the quantitative case of conservation biology, foreign country's EIA and domestic ecology. Among them, a few factors was extracted. To applicate the criteria to domestic forest ecosystem, expert opinion survey was executed to the ecologist. The results were summarized as follows; 1. Classification of sites was made of land use system which is related to forest ecosystem or forest conservation. Sites are divided into 3 categories which are nature preservation area, seminature preservation area and urbanized area. Evaluation criteria is consisted of rarity and naturalness. 2. Each area had different criteria composition according to the site characteristics. Criteria of nature preservation area is rarity in the broad sense (distribution pattern of vegetation), vegetation size, successional stage and depth of organic matters. Those of seminature preservation area are rarity in the broad sense (distribution area of vegetation), vegetation size, successional stage, diameter at breath height and depth of organic matters. And those of urbanized area are vegetation distribution in area, successional stage, age of forest and diameter of breath height. The basic data of criterion was gathered by field survey. 3. Evaluation index and total naturalness index was obtained by adding the each criterion. It is made up of two categories-rarity and naturalness. TNi is divided into 3 grades. Grade I is more than 70% for TNi, grade IT is 50~70%, and grade III is below 50%. According to the each grade, permitted action and facilities were suggested.. This research just focuses on the evaluation of vegetation quality and the assessment results do not directly judge conservation or development. To make better evaluation criteria, various fields of forest ecosystem-geological or physical nature environment and fauna ecosystem etc. -will be added wholly to this research.
Objectives The purpose of this study is to understand the intention expressed as Jeubjeubhanchul(濈濈汗出) unlike the aspect of perspiration in 『Sanghanlun(傷寒論)』 and 『Donguibogam(東醫寶鑑)』. Methods We examined Sibjo-tang(十棗湯) and the aspect of perspiration focusing on the Chip(漐) and Jeub(濈) in 『Sanghanlun(傷寒論)』, 『Donguibogam(東醫寶鑑)』, and 『Donguisusebowon(東醫壽世保元)』. Moreover, we examined the connection between the quoted purpose of Sibjo-tang and the aspect of perspiration. Results and Conclusion 1. When Leejema quoted Sibjo-tang to explain the Chestbind disease(結胸) of the Soyang pattern, he used Jeubjeubhanchul (濈濈汗出) instead of Chipchiphanchul(漐漐汗出). 2. About the aspect of perspiration in 『Sanghanlun』 and 『Donguibogam』, the Chip(漐) is used in a situation when the disease progressed from Pyo(表) to Li(裏) and Taeyang-byeong(太陽病) to Yangmyung-byeong(陽明病), and it is expression of physiological perspiration. The Jeub(濈) is used in a situation related to interior(內), Li(裏), and Yangmyung-byeong. 3. Leejema understood Sibjo-tang in relation to the Chestbind disease of the Soyang pattern caused by aggravation of Liyeol (裏熱). Therefore, Leejema used the Jeub(濈) related to interior, Li and Yangmyung-byeong, and it is expression of perspiration caused by aggravation of Liyeol in the Soyang pattern. 4. At first time, Leejema mentioned perspiration and short of breath(短氣) in the symptoms of Sibjo-tang, but he mentioned dry vomiting(乾嘔) and short of breath in 『Sinchukbon(辛丑本)』. Thus, his perspective of perspiration related to Sibjo-tang was appeared in 『Gabobon(甲午本)』, and he paid attention to perspiraion as a major symptom of the Chestbind disease in the early. After clinical experience was built up, It was not that important as the past.
To compare the accuracy of breath-hold magnetic resonance imaging sequences to establish the most effective superparamagnetic iron oxide-enhanced sequence for detection of hepatic metastases. A total of 100 patients(50men and 50women, mean age: 60years) with liver disease(including malignant and benign liver lesions) were investigated at 3.0T machine (GE, General Electric Medical System, Excite HD) with 8Ch body coil. Pulse sequence for MR imaging decided to the FS-T2-FSE-RT(TR/TE/Thick./Freq./Phase=12857ms/100ms/7mm/512/384), MGRE(TR/TE/Thick./Freq./Phase=100ms/9.7ms/7mm/384/288), in-out of phase echo(TR/$TE_1$, $TE_2$/Thick./Freq./Phase=140ms/2.4, 5.8ms/7mm/352/300), Images obtained before the injection of SPIO. Six sequences were optimized for lesion detection: FS-T2-FSE-RT, multigradient recalled echo data image(MGRE), T2-weighted MGRE with an 9.7msec echo time. Images were reviewed independently by five blinded observers. The accuracy of each sequence was measured by using picture archiving communication system analysis. All results were correlated with findings at multidectator computed tomography examination. Differences between the mean results of the six observers were measured by using paired student t-test analysis. Postcontrast T2-weighted MGRE sequences were the most accurate and were significantly superior to postcontrast FS-T2-FSE-RT, T2-weighted MGRE, in-out of phase MR sequences(p < .05). For all lesions that were malignant or smaller than 1 cm, respectively, contrast to noise ratio of pre and postcontrast sequences were -1and -0.3 for T2-weighted FSE, 0.53 and 4.5 in-out of phase, 7, 7.08, 5.08, 3.32, 1.7, 1.16, 0.79, 0.68 for GRE with 2.9, 7.5, 12.1, 16.6, 21.2, 25.8, 30.4, 35.0 TE values. Breath-hold various TE precontrast sequences offer improvement in sensitivity compared with fixed multigradient recalled echo sequences alone.
Objective : This study is purposed to report 2 cases of cancer patient whose symptoms related to radiation pneumonitis had been controlled with Saengmaek-san treatment. Methods : A 56-year-old female rectal cancer patient was prescribed with Saengmaek-san due to the symptoms which had developed 4 weeks after the completion of her radiotherapy session in both lungs. Her chief complaints were shortness of breath, dry cough and fatigue. Another case, 53-year-old male patient with hepatocellular carcinoma, had also developed symptoms of fatigue, weight loss and dry cough after his radiotherapy session in left upper lung zone. Radiological changes of both patients' chest X-ray suggested radiation pneumonitis. Results : Symptoms of the female patient were improved, especially shortness of breath, after Saengmaek-san treatment, without any aggravation in her chest X-ray result. However, infiltrative opacity in the left upper lung zone of the male patient was aggravated despite the improvement of his clinical symptoms. His remaining symptoms and radiological change were effectively controlled after steroid therapy. Conclusion : Seangmaek-san may be considered as a potential treatment for symptoms related to radiation pneumonitis with proper monitoring.
To develop a $^{13}C-urea-containing$ capsule for more economic and sensitive diagnosis of Helicobacter pylori infection, the $^{13}C-urea-containing$ capsules were prepared with various additives such as polyethylene glycol, microcrystalline cellulose, sodium lauryl sulfate and citric acid. Their dissolution test and $^{13}C-urea$ Breath Test in human volunteers were then performed. Polyethylene glycol increased the initial dissolution rates of urea and difference ${\sigma}$$^{13}C$ values from $^{13}C-urea$, while microcrystalline cellulose and sodium lauryl sulfate decreased them. Irrespective of addition of citric acid, the compositions with polyethylene glycol showed higher values from $^{13}C-urea$ compared to a commercial 76 mg $^{13}C-urea-containing$ capsule due to higher initial dissolution rate. The capsules with 38 mg $^{13}C-urea$ and 1.9 mg polyethylene glycol, which showed higher Helicobacter pylori-positive value of about $8{\textperthousand}$ at 10 min, improved the sensitivity of $^{13}C-urea$ in human volunteers. Thus, the $^{13}C-urea-containing$ capsule with polyethylene glycol would be a more economical and sensitive preparation for diagnosis of Helicobacter pylori infection.
The purpose of this study was to find out the degree of self-consciousness of oral malodor, the status of oral hygiene care, some oral examination factors, and measurement values of malodor compounds through conduction oral examination and questionnaire survey, and measuring volatile malodor compounds by instrumental analysis. The data were collected from 155 patients visiting a dental clinic by using a self-administered questionnaire, conducting oral examination for halitosis, and measuring malodor compounds with Oral Chroma. The rate of recognizing their breath as 'somewhat bad' were 80.0% for the female patients and 74.3% for the male, and, however, 14.3% of the male recognized their breath as 'very bad' while 18.8% of the female did not recognized oral malodor, showing statistically significant difference between gender(p=0.004). The average concentrations of volatile sulfur compounds measured by Oral Chroma were 1.65 ng/10 ml for hydrogen sulfide, 1.71 ng/10 ml for methyl mercaptan and 1.66 ng/10 ml for dimethyl sulfide, on the average, respectively, exceeding malodor threshold levels of all 3 compounds, and were significantly higher in the male group than those in the female, also exceeding all threshold levels except hydrogen sulfide values of the female group. The type of oral malodor was the most prevalent for Type I as 23.2%, followed by Type V, Type IV, Type II whileas Type I and Type IV in the female as 30.6% and 25.9% respectively, showing statistically significant difference by gender(p=0.006). The correlations among oral examination indices was the highest between tongue fur score and simplified oral hygiene index, followed by a significant reverse correlation between the number of fixed prosthodontic teeth and the number of dental caries(p=0.000).
Idiopathic Parkinson's Disease patients' speech is hypokinetic dysarthria and their speech is possibly the consequence of impaired respiratory support. The purpose of this study was focused on the respiratory characteristics of speech breathing in de novo IPD who were not given prior vocal or anti-Parkinson treatment. A total of 40 subjects participated in the study: 20 de novo IPD patients between the ages of 50 and 80, and 20 normal subjects with similar age, height, and weight matches. Forced Expiratory Vital Capacity (FVC), Forced Expiratory Volume in 1 sec (FEV1) and $FEV_1$ as a percentage of FVC (FEV1/FVC) was measured with a PC-based spirometer (Cosmed). In addition, Maximum Phonation Time (MPT), Mean Airflow Rate (MFR), Subglottal Pressure (Psub) and the number of syllables produced per breath were measured with a Phonatory Aerodynamic System (Kay PENTAX). All subjects were asked to read a standardized Korean paragraph and the following measurements were obtained from the task. Results indicated no statistically significant differences in respiratory function (FEV1/FVC%) and aerodynamic function between the two groups, but the number of syllables per breath was significantly lower in the IPD patient group than in the normal group and it could be predicted by FVC and MFR. Therefore, the study shows that the MFR from the lungs during speech in de novo IPD patients is used inefficiently.
Objectives The purpose of this study is to find out whether or not there are differences of effects when we count breaths on inspiration or expiration, and how differently the effects show up according to gender or Sasang Constitution Methods A group of 79 participants were enrolled and diagnosed Sasang Constitution by Sasang Constitution Diagnosis System of the Korea Institute of Oriental Medicine. Physical responses to respiratory changes were measured by Biofeedback sensors that were attached to the participants while they were seated comfortably on a sofa and breathing normally without counting for 5 minutes, counting on inspiration for 5 minutes, and counting on expiration for 5 minutes. Results and Conclusions 1. Mean heart rate and respiration rate decreased in association with breathing counting on inspiration and expiration more than breathing normally without counting. Especially respiration rate was stabilized during counting on inspiration and mean heart rate was stabilized during counting on expiration. 2. Interaction between breathing methods and gender did not appear. In comparison between gender, LF/HF ratio was higher in male during breathing normally without counting and counting on inspiration. Body temperature was higher in male during counting on inspiration and expiration. 3. Interaction between breathing methods and Sasang Constitution did not appear. In comparison between Sasang Constitution, mean heart rate was higher in Soyang-in, Soeum-in than Taeeum-in and abdominal amplitude was lower in Taeeum-in than Soeum-in.
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