• 한국수산과학회지
• /
• 제49권6호
• /
• pp.838-844
• /
• 2016

In this study, epidemiological cut-off values were estimated for 44 Photobacterium damselae isolates, since clinical breakpoints have not been established for this pathogenic bacterium. The susceptibility of the isolates to 10 antibiotics was evaluated using internationally standardized disc diffusion protocols. Normalized resistance interpretation was used to generate statistically valid epidemiological cut-off values for the susceptibility data. There were not enough strains exhibiting full sensitivity to ampicillin and amoxicillin to allow analysis of these antibiotics. Because there were only a marginally sufficient number of strains exhibiting full sensitivity to oxytetracycline, the cut-off value generated provided only a provisional estimate. The valid wild-type cut-off values were <13, 13, 9, 22, 25, 27, and 28 mm for gentamicin, cephalexin, erythromycin, oxolinic acid, flumequine, florfenicol, and sulfamethoxazole/trimethoprim, respectively. The application of these cut-off values should greatly facilitate the rational selection of antibiotics for use in commercial fish farms.

• 약학회지
• /
• 제52권3호
• /
• pp.219-224
• /
• 2008

Clinically isolated methicillin-resistant Staphylococcus aureus strains were exposed to subinhibitory concentration of DW286, DW-224a, gemifloxacin, trovafloxacin, sparfloxacin and ciprofloxacin during 26- to 39-days period. Subculturing led to resistance development, and most of the selected mutants were above susceptible breakpoints. Selected mutants had broad cross resistance to other quinolone antibiotics and only one mutant was completely susceptible to all fluoroquinolones. Twenty five among 42 mutants revealed mutations on DNA gyrase and topoisomerase IV by sequencing. Also 16 mutants had fluoroquinolones MICs that were 4-32 times lower in the presence of reserpine. In conclusion, alterations in DNA gyrase or topoisomerase IV and action of efflux pumping out system are the resistance mechanisms of DW-224a.

• The Journal of Asian Finance, Economics and Business
• /
• 제8권10호
• /
• pp.239-247
• /
• 2021

Central Bank authorities should carefully manage inflation rate uncertainties to achieve economic growth and development not only in the short-run but also in the long-run. Since inflation is a key macroeconomic variable, an increased understanding about its behavior is undoubtedly important. Thus, paper employs unit root with breakpoints to examine the mean reverting behavior of inflation rate in the Philippines using monthly data from 2002 to 2020. Empirically, the unit root breakpoint innovational and additive outlier tests favor the stationarity or mean reverting behavior of inflation in the Philippines. Also, results of standard unit root tests, ADF, PP, GLS-Dickey-Fuller, KPSS and NP, provide strong evidence of mean reverting processes. The mean reverting behavior of inflation rate reveals that the monetary policy using inflation targeting framework has succeeded in reducing chronic inflation persistence in the Philippines. Thus, this research supports inflation targeting policy that aims to maintain general price level stability for the Philippine economy's long-term growth and development prospects. The findings of this research remain important for the central bankers for not only providing them better understanding about the behavior of inflation rate, but also helping them formulate and implement policy reforms related to money, credit and banking.

• 정보과학회 논문지
• /
• 제41권10호
• /
• pp.832-837
• /
• 2014

프로그램의 로딩 속도는 프로그램이 요청하는 디스크 블록을 미리 읽어 들임으로써(프리페칭) 향상시킬 수 있다. 그러나 기존의 프리페칭 관련 기법들은 특정 프로그램에 최적화된 경우를 제외하면 상당한 오버헤드를 보여주었다. 특히 요청블록을 정확히 추적하는데 어려움이 있었다. 어떤 블록들은 여러 시퀀스(단위시간 내에 추적된 블록들)에 나타날 수 있고 두 접근 시퀀스가 동일 하더라도 버퍼 캐시에 의해서 접근 시간과 수집되는 블록 정보가 다를 수 있기 때문에 분석이 까다롭다. 본 논문에서는 소프트웨어적 접근 방법으로 새로운 범용 워크로드 프리페칭 기법을 제안한다. 제안하는 프리페칭 기법은 브레이크 포인트를 프로그램의 적재 적소에 배치함으로써 요청 블록의 상관관계 정보를 수집하고, 이를 바탕으로 프리페칭을 수행한다. 상용 하드디스크를 이용한 실험 결과, 불필요한 오버헤드가 감소되었으며 기동 시간은 평균 30%, 로딩은 평균 15% 단축되었음을 확인하였다.

• Journal of Genetic Medicine
• /
• 제11권1호
• /
• pp.16-21
• /
• 2014

A 31-year-old woman, who was pregnant with twins, underwent chorionic villus sampling because of increased nuchal translucency in one of the fetuses. Cytogenetic analysis showed a normal karyotype in the fetus with increased nuchal translucency. However, the other fetus, with normal nuchal translucency, had a derivative X chromosome (der(X)). For further analysis, fluorescence in situ hybridization (FISH) and additional molecular studies including fragile X analysis were performed. FISH analysis confirmed that the Y chromosome was the origin of extra segment of the der(X). The X-chromosome breakpoint was determined to be at Xq27 by FMR1 CGG repeat analysis, and the Y-chromosome breakpoint was determined to be at Yq11.23 by the Y chromosome microdeletion study. To predict the fetal outcome, the X-inactivation pattern was examined, and it revealed non-random X inactivation of the der(X). To the best of our knowledge, the identification of an unbalanced Xq;Yq translocation at prenatal diagnosis has never been reported. This study was performed to identify precise breakpoints and the X-inactivation pattern as well as to provide the parents with appropriate genetic counseling.

• Yonsei Medical Journal
• /
• 제59권9호
• /
• pp.1115-1122
• /
• 2018

Purpose: To investigate biomechanical properties of the cornea using a dynamic Scheimpflug analyzer according to age. Materials and Methods: In this prospective, cross-sectional, observational study, participants underwent ophthalmic investigations including corneal biomechanical properties, keratometric values, intraocular pressure (IOP), and manifest refraction spherical equivalent (MRSE). We determined the relationship of biomechanical parameters and ocular/systemic variables (participant's age, MRSE, IOP, and mean keratometric values) by piecewise regression analysis, association of biomechanical parameters with variables by Spearman's correlation and stepwise multiple regression analyses, and reference intervals (RI) by the bootstrap method. Results: This study included 217 eyes of 118 participants (20-81 years of age). Piecewise regression analysis between Corvis-central corneal thickness (CCT) and participant's age revealed that the optimal cut-off value of age was 45 years. No clear breakpoints were detected between the corneal biomechanical parameters and MRSE, IOP, and mean keratometric values. Corneal velocity, deformation amplitude, radius, maximal concave power, Corvis-CCT, and Corvis-IOP exhibited correlations with IOP, regardless of age (all ages, 20-44 years, and over 44 years). With smaller deformation amplitude and corneal velocity as well as increased CorvisIOP and Corvis-CCT, IOP became significantly increased. We provided the results of determination of confidence interval from RI data using bootstrap method in three separate age groups (all ages, 20-44 years, and over 44 years). Conclusion: We demonstrated multiple corneal biomechanical parameters according to age, and reported that the corneal biomechanical parameters are influenced by IOP.

• 산업경영시스템학회지
• /
• 제44권1호
• /
• pp.26-36
• /
• 2021

The topic of this study is the field of humanitarian logistics for disaster response. Many existing studies have revealed that compliance with the golden time in response to a disaster determines the success or failure of relief activities, and logistics costs account for 80% of the disaster response cost. Besides, the agility, responsiveness, and effectiveness of the humanitarian logistics system are emphasized in consideration of the disaster situation's characteristics, such as the urgency of life-saving and rapid environmental changes. In other words, they emphasize the importance of logistics activities in disaster response, which includes the effective and efficient distribution of relief supplies. This study proposes a mathematical model for establishing a transport plan to distribute relief supplies in a disaster situation. To determine vehicles' route and the amount of relief for cities suffering a disaster, it mainly considers the urgency, effectiveness (restoration rate), and uncertainty in the logistics system. The model is initially developed as a mixed-integer nonlinear programming (MINLP) model containing some nonlinear functions and transform into a Mixed-integer linear programming (MILP) model using a logarithmic transformation and piecewise linear approximation method. Furthermore, a minimax problem is suggested to search for breakpoints and slopes to define a piecewise linear function that minimizes the linear approximation error. A numerical experiment is performed to verify the MILP model, and linear approximation error is also analyzed in the experiment.

• Journal of Genetic Medicine
• /
• 제2권1호
• /
• pp.11-15
• /
• 1998

This paper reports 3 cases with 46,XX sex reversed male. Three 46,XX hypogonadal subjects showed complete sex reversal and had normal phallus and azoospermia. We studied them under clinical, cytogenetic and molecular aspects to find out the origin of the sex reversal. Patients had markedly elevated serum follicle-stimulating hormone (FSH) and lutenizing hormone (LH) and decreased or normal range of serum testosterone. The testicular volumes were small (3-8ml). Testicular biopsy showed Leydig cell hyperplasia and atrophy of seminiferous tubules. We obtained the results of normal 46,XX, and the presence of Y chromosome mosaicism was ruled out through XY dual fluorescent in situ hybridization (FISH). By using polymerase chain reaction (PCR), we amplified short arm (SRY, PABY, ZFY and DYS14), centromere (DYZ3), and heterochromatin (DYZ1) region of the Y chromosome. PCR amplification of DNA from these patients showed the presence of the sex-determining region of the Y chromosome (SRY) but didn't show the centromere and heterochromatin region sequence. The SRY gene was detected in all the three patients. Amplification patterns of the other regions were different in these patients; one had four amplified loci (PABY+, SRY+, ZFY+, DYS14+), another had two loci (SRY+, ZFY+) and the other had two loci (PABY+, SRY+). We have found that each patient's translocation elements had different breakpoints at upstream and downstream of the SRY gene region. We conclude that the testicular development in 46,XX male patients were due to insertion or translocation of SRY gene into X chromosome or autosomes.

• Genomics & Informatics
• /
• 제15권3호
• /
• pp.87-97
• /
• 2017

Multiple myeloma (MM) is a malignant disease caused by an abnormal proliferation of plasma cells, of which the prognostic factors include chromosomal abnormality, ${\beta}$-2 microglobulin, and albumin. Recently, the term chromothripsis has emerged, which is the massive but highly localized chromosomal rearrangement in response to a one-step catastrophic event. Many studies have shown an association of chromothripsis with the prognosis in several cancers; however, few studies have investigated it in MM. Here, we studied the association between chromothripsis-like patterns and treatment resistance or prognosis. First, we analyzed nine MM cell lines (U266, MM.1S, RPMI8226, KMS-11, KMS-12-BM, KMS-12-PE, KMS-28-BM, KMS-28-PE, and NCI-H929) and bone marrow samples of four patients who were diagnosed with MM by next-generation sequencing-based copy number variation analysis. The frequency of the chromothripsis-like pattern was observed in seven cell lines. We analyzed the treatment-induced chromothripsis-like patterns in KMS-12-BM and KMS-12-PE cells. As a result, breakpoints and chromothripsis-like patterns were increased after drug treatment in the relatively resistant KMS-12-BM. We further analyzed the patients' results according to the therapeutic response, which was divided into sensitive and resistant, as suggested by the International Myeloma Working Group. The chromothripsis-like pattern was more frequently observed in the resistant group. In the sensitive group, the frequency of the chromothripsis-like pattern decreased after treatment, whereas the resistant group showed increased chromothripsis-like patterns after the treatment. These results suggest that the chromothripsis-like pattern is associated with treatment response in MM.

• 한국환경과학회지
• /
• 제11권4호
• /
• pp.347-353
• /
• 2002

본 연구의 시료는 현재 서울특별시와 수도권 도시들에서 상수원수로 이용하고 있는 한강수를 대상으로 수행되었다. 배ㆍ급수계통의 2차 소독을 위한 클로라민소독의 특성에 관하여 고찰하였으며 그 결과는 다음과 같다. 1) $25^{\circ}C$, Cl$_2$ / NH$_3$-N비 1:1 ~ 14:1에서 PH6, 7, 8의 불연속점은 각각 Cl$_2$ / NH$_3$-N비 11:1, 9:1, 10:1이었다. pH6의 경우, Cl$_2$ / NH$_3$-N비가 9:1일 때 총 잔류염소량이 최대인 반면, pH 7, 8의 경우 Cl$_2$ / NH$_3$-N비가 각각 6:1, 5:1 일 때 최대가 되었다. 2) pH가 6, 7, 8로 증가함에 따라 모노클로라민이 최대로 생성되는 Cl$_2$ / NH$_3$-N비는 7:1에서 5:1로 이동하였으며, pH6 ~ 8 범위에서 pH가 증가함에 따라 모노클로라민의 생성량이 많았다. 3) 클로라민의 분해는 Cl$_2$ / NH$_3$-N비가 증가하고 pH가 낮을수록 증가하였다. 클로라민의 잔류성은 유리염소보다 월등히 좋았다. pH6에서 모노클로라민과 디클로라민이 생성된 이후, 접촉시간이 경과함에 따라 디클로라민의 분포비가 증가하였다.