• 약학회지
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• 제22권4호
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• pp.219-225
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• 1978

From the dried roots of Caragana chamlagu Lamarck which have been known to be effective against neuralgia, arthritis, migraine, and sputum, etc. in the folk medicines of Korea, a colorless sterol-like substance, C$_{29}H_{50}O$, m.p. $115-117^{\circ}$, was isolated from the non-saponified fraction of methanolic extract. It was confirmed to be a mixture of four sterols, namely cholesterol, brassicasterol, campesterol, and $\beta$-sitosterol by physico-chemical properties, ir, nmr, mass spectra and gas liquid chromatographic method with the comparison of the authentic samples. In mass fragments of four sterols, furthermore, the same ions of m/e 273, 255, 229 and 213 were assigned to fragments of stigmastane skeletons of the unsaturated phytosterol, and the characteristic ions of each sterol were observed 386, 371, 368, 353 in cholesterol, 398, 383, 380, 365, 355, 337 in brassicasterol, 400, 385, 382, 367 in campesterol and 414, 399, 396, 381 in $\beta$-sitosterol, respectively.

• Journal of Applied Biological Chemistry
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• 제53권4호
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• pp.207-211
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• 2010

Kohlrabi 새싹을 100% MeOH을 이용하여 추출하였고, 그 추출물을 EtOAc, n-BuOH 및 $H_2O$을 이용하여 분획을 나누고 감압농축 하였다. EtOAc에 대한 silica gel과 ODS column chromatography를 반복 수행하여 3개의 sterol 화합물을 분리하였다. 분리한 물질의 물리적, 화학적, 및 NMR, MS, IR과 같은 분광학적 데이터를 측정하였고, 그것을 해석하여 sterol 화합물인 ${\beta}$-sitosterol(1), brassicasterol(2) 및 7-ketobrassicasterol (3)로 구조동정 하였다. 화합물 1은 대부분 식물에서 발견되는 물질이다. 화합물 2는 배추과 식물에서 발견되는 물질이며, 화합물 3은 식물에서 드물게 발견되는 물질이다.

• 대한가정학회지
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• 제28권2호
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• pp.31-36
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• 1990

This studies were conducted to find out the possibility of utilizing red pepper seed as resources of food fats, the research method was designed to make a comparison between crude and refined oil, and the results of the studies are as follows : The red pepper seed contained 28% of crude fat and 21% of crude protein. The main fatty acids of red pepper seed oil were linoleic acid(72.10~72.31%), palmitic acid(12.81~13.28%) and oleic acid(9.47~10.48%). The linolenic acid content was so small that is will not influence the autoxidation of the red pepper seed oil. The major triglyceride type of crude and refined oil of red pepper seeds were C52 and C54. The other types were found in a small quantity. The sterol composition of crude oil was $\beta$-sitosterol, campasterol, stigmasteral and brassicasterol,in the quantity order. after refining, brassicasterol was not detected, and the content was decreased by one six and one eight. The toropherol composition of crude and refined oil, tocopherol analog was composed of three kinds $\alpha$-, ${\gamma}$-, $\delta$-, but no $\beta$-form. the quantity of ${\gamma}$-, $\alpha$- and $\delta$-tocopherol were 162.91, 83.72, 43.98mg% respectively. The Quantity of and capsaicin in crude oil was 1,296 ppm, and it was reduced consicerably by refining and removed completely after the process of redeodorization.

• 생약학회지
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• 제36권1호통권140호
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• pp.34-37
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• 2005

Reactive oxygen species (ROS) are known to cause oxidative modification of DNA, proteins, lipids and small cellular molecules and are associated with tissue damage and are the contributing factors for inflammation, aging, cancer, arteriosclerosis, hypertension and diabetes. We screened the anti-oxidants in V79-4 hamster lung fibroblast cells induced by hydrogen peroxide with eighteen pure compounds isolated from natural products. Allantoin, brassicasterol, and hypaconitine were found to strongly scavenge intracellular reactive oxygen species, which is measured by dichlorodihydrofluorescin diacetate method (DCHF-DA), and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical.

• Journal of Nutrition and Health
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• 제10권2호
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• pp.44-53
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• 1977

The Korean origin edible oil sterol part of the rape, sesame and perilla oil can be separated from the other constituents of the non-saponifiable fraction, by the thin layer chromatography on the preparative Plates. The composition of sterols have been determined by gas liquid chromatography and thin layer chromatographic anlysis. Two sterols, ${\beta}$-sitosterol and campesterol were present in all of the oils. And brassicasterol were found in rapeseed oil in addition to the two sterols that were common to all of the oils studied. It was noted that ${\beta}$-sitosterol was the major sterol in the Korean original edible oils. The results showed that contents of sterols were campesterol 24.31%, ${\beta}$-sitosterol 58.90% ana Brassicasterol 11.54%, and $\Delta^7$-sterol 5.25% by method of triangulation and campesterol 26.16%, ${\beta}$-sitosterol 57.50%, brassicasterol 11.70% and ${\Delta}^7$-sterol 4.64% by method of Planimetry of rape seed oil. By sesame seed oil sterol compositions were campesterol 20.35%, stigmasterol 9.15%, ${\beta}$-sitosterol 43.49%, ${\Delta}^7$-sterol 11.25% and others 15.76% by method of triangulation and campesterol 16.79%, stigmasterol 8.69%, ${\beta}$-sitosterol 44.58%, ${\Delta}^7$-sterol 14.28% and others 15.56% by method of planimetry. Campesterol 12.45%, stigmasteriol 5.40%, ${\beta}$-sitosterol 72.32% and ${\Delta}^7$-Sterol 9.83% by method of triangulation-and campesterol 13.00%, stigmasterol 3.76%, ${\beta}$-sitosterol 74.57% and ${\Delta}^7$ sterols 8.67% by method of planimetry of perilla oil. Contents of totalsterol in Korean edible oils were 0.82% by rape, 0.58% by sesame and 0.45% by perilla, respectively.

• 한국작물학회지
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• 제48권
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• pp.24-30
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• 2003

Plant fats and oils contain phytosterols as naturally occurring constituents, which are present in pure or esterified form, or conjugated as glycosides. The predominant phytosterol is sitosterol and minor components are campesterol, stigmasterol, $\Delta^7$-avenasterol and brassicasterol. They play a key role in cell membrane function of plants as cholesterol in animals. Phytosterols have been known for many years for their properties for reducing blood cholesterol levels, as well as their other beneficial health effects for cancer preventive biological-active substances. The objective of this paper is to present a description of the recent advances in phytosterols for future research.

• 한국수산과학회지
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• 제16권3호
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• pp.255-259
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• 1983

개불지질의 성상을 밝힐 목적으로 그 지방산조성 및 sterols 조성에 대하여 분석 검토 하였는데, 그 결과는 다음과 같다. (1) 조지질과 부검화물함량은 각각 $1.9\%,\;14.3\%$였으며, 요오드값은 111이었다. (2) 조지질의 조성은 인지질이 $39.8\%$, 유리 sterols이 $27.7\%$, triglyceride가 $21.4\%$였다. (3) 지방산조성은 $C_{20:4}$가 $19.4\%$로 가장 많았고, 다음으로 $C_{16:0},\;C_{20:1},\;C_{18:1}$이 각기 $13.7\%,\;11.3\%,\;10.4\%$였으며 또 polyene산 monene 산 및 포화산으로 계산하면 각각 $39.7\%,\;31.9\%$ 및 $26.6\%$였다. (4) 5종의 sterols이 검출되었는데 cholesterol이 $57.6\%$로 가장 많았고 다음이 brassicasterol이 $20.3\%$, 24-methylene cholesterol of $17.7\%$, 22-dehydro cholesterol of $3.0\%$, 22-trans-24-norcholesta-5,22-dien-3-${\beta}$-ol 이 $1.4\%$였다.

• 한국수산과학회지
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• 제9권3호
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• pp.185-193
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• 1976

패류의 스테롤조성은 복장하나 이들 스테롤의 유래(由來)에 관한 연구는 없다. 이들 스테롤의 기원에 관한 연구로서 우선 2매패(二枚貝)인 개량조개와 북방조개, 권패(卷貝)연 전복, 함수산(鹹水産) 소라의 스테롤 조성을 조사한 결과 다음과 같은 결과를 얻었다. 1. 각 시료의 스테롤의 함량을 보면 2매패인 개량조개 및 복방조개가 총지질에 대하여 각각 $12.0\%,\;11.8\%$로서 권패인, 전복의 $16.2\%$ 소라의 $15.3\%$ 보다 약간 낮았다. 2. $15\%$ $AgNO_3-Silica$ Gel HF254 TLC 상에서 조성이 복잡한 스테롤을 상호 분리할 수 있었다. 3. 2매패인 개량조개 및 북방조개의 스테롤 조성은 C-26의 스테롤인 22-트란스-24-놀 코레스타-5.22-디엔-3-$\beta$을 이 각각 $3.0\%,\;3.9\%$, 22-데하이드로 코레스테롤이 $6.7\%,\;10.2\%$, 콜레스테롤이 $39.0\%\;48.6\%$, 브라시카스테롤이 $14.0\%,\;13.8\%$, 24-메치렌코레스테롤이 $10.0\%,\;11.9\%$, 스티그마스스테롤이 $2.4\%$, 소량, $\beta$-시토스테롤이 $10.5\%,\;11.9\%$, 프코스테롤 4.3\%, 소량이었고, 북방 대합에는 구조 미상의 스테롤이 소량 함유되어 있었다. 4. 권패인 전복 및 소라에는 코레스테롤이 각각 $98.0\%,\;97.5\%$였고, 22-데하이드로 코테스테롤, 브라시카 스테롤이 미량 함유되어 있다. 또 전복에는 이외 24-메치렌코레스테로, 프코스테롤이 미량 함유되어 있었다. 5. 2매패에는 코레스테롤이 $50\%$ 미만인데 대하여 권패에는 코레스테롤이 $98\%$ 정도로, 2매패와 권패의 스테롤조성상에 차가 현저히 보였다.

• 한국응용과학기술학회지
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• 제10권2호
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• pp.19-27
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• 1993

Levels of sterols including ${\Delta}^7$-dehydrogenase isolated from the tissues of marine animal products (20 species) were determined on 1.5% OV-17 columm of gas-liquid chromatography. The composition showed that the mussels and clams contained various sterols in their tissues : cholesterol, brassicasterol. 24-methylenecholesterol with some minor components such as 22-trans-norcholesta-5,22-dien-3${\beta}$-ol, 22-cis-dehydrocholesterol, 22-trans-dehydrocholesterol, desmosterol, 7-dehydrocholesterol, campesterol, stigmasterol, ${\beta}$-sitosterol, isofucosterol, and 7-dehydrocholesterol which could be converted into vitamin $D_3$ in the skin tissue of animal was present in the muscle of oyster, Crassostrea gigas. On the other hand, the others including gastropoda were predominantly composed of cholesterol. The minor sterols such as 24-methylenecholesterol, stigmasterol and ${\beta}$-sitosterol in the fish intestines are supposed to be derived from dietary plankton. Cholesterol ${\Delta}^7$-dehydrogenase which could convert cholesterol into ${\Delta}^7$-dehydrogenase was present in the pickles of Tricurus haumela intestine.

• 한국수산과학회지
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• 제38권2호
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• pp.118-128
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• 2005

Surface sediment samples from 31 stations in Jinhae Bay were analyzed to evaluate the pollution by sewage from the spatial distribution of the fecal sterol, coprostanol. The sums of eight sterols (coprostanol, epicholestanol, epicoprostanol, cholesterol, cholestanol, brassicasterol, stigmasterol and $\beta$-sitosterol) were in the range of $2,703-27,154{\cal}ng/{\cal}g$ dry weight. The concentrations of the coprostanol in the sediments, which is a good tracer of sewage-derived organic contamination, were in the range of $76-3,964{\cal}ng/{\cal}g$ dry weight. The levels of coprostanol were much higher (almost one order of magnitude) at stations close to the big cities such as Masan and Jinhae. However, those levels were comparable to or lower than those previously reported in other foreign locations. Some ratios of coprostanol to other sterols and multivariate analysis could provide us with the information on the origin of the sterols being from sewage, plankton, and/or benthos. Those showed sewage dominance at the stations near the cities Masan and Jinhae, plankton dominance at the center of Jinhae Bay and mainly marine fauna origin at the mouth of Jinhae Bay. These results suggest that the contents of sterols and some ratios of these components are quite powerful tracer for the detection of the origin of the organic contaminants in the coastal environments.