• Journal of Nutrition and Health
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• 제29권8호
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• pp.849-860
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• 1996

The supply of different fatty acids during the development period has significant effects. This study examined the effects of dietary $\omega$3 and $\omega$6 fatty acid compositions on phospholipids (PLs) of RBC and rat brain subcellular fractions (synaptosome, microsome, mitochondria), and on learning ability of the 2nd generation rat. Rats were fed experimental diets 3-4 wks prior to the conception. Early in the lactation period, the feeding mothers were exchanged. Diets consisted of 10% fat(by weight), which was either safflower oil('S') poor in $\omega$3 fatty acids or computer-searched mixed oil('M') with P/M/S ratio, 1/1.4/1 and $\omega$6/$\omega$3 ratio, 6.1/1. The 'S' and 'M' rats were subdivided further into SS, SM, MS & MM rats according to their lactation stauts. At 3 (weaning) & 9 wks of age, the percentage of total $\omega$3 fatty acids to their lactation status. At 3 (weaning) & 9 wks of age, the percentage of total $\omega$3 fatty acids and the ratios of $\omega$3/$\omega$6 fatty acids in PLs of RBC and brain subcellular fractions in SM and MM groups fed milk from the mixed oil-fed mothers for 2 wks tended to be higher than those in SS and MS groups respectively. In contrast, the concentrations of $\omega$6 fatty acids, especially 22:5$\omega$6 in all fractions, were significantly lower in the SM & MM groups compared to those of the SS & MS groups respectively. In contrast, the concentration of $\omega$6 fatty acids, especially 22:5$\omega$6 in all fractions, were significantly lower in the SM & MM groups compared to those of the SS & MS groups, The values for the DHA$\omega$3/22:5$\omega$6 ratios after the lactation period were markedly higher in the groups (SM & MM) which were reared by mixed oil(MO) fed mothers. In carring out Y-water maze at 9th wk of age, the SM(4.2$\pm$0.5) & MM (5.3$\pm$0.5) groups made significantly less errors compared to the SS(6.2$\pm$0.6, p<0.05 compared with SM) & MM (7.2$\pm$0.5, p<0.05 compared with MM) groups which were lactated by the safflower oilfed mothers. Therefore, by feeding a balanced fatty acid diet from the lactation period up to 9 wks of age as compared with the groups fed $\omega$3 fatty acid-deficient diet regardless of mother's diet given before parturition. The levels of DHA(synaptosome) and 22:5$\omega$3 (mitochondria) were positively correlated not only with these values in RBC but also with visual discriminating ability. The levels of DHA and 22:5$\omega$3 in RBC can, therfore, reflect visual discriminatng ability in the rat.

• 한국식품영양과학회지
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• 제33권10호
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• pp.1626-1633
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• 2004

$\omega$3계 지방산이 부족한 50군과 P/M/S 및$\omega$6/$\omega$3비율이 적절한 MO군으로 생후 9주까지 사육한 흰쥐의 적혈구와 뇌조직의 시냅토솜, 미토콘드리아 및 마이크로솜내 oleic acid 조성 비율은 생후 3주에는 두 군간에 유의한 차이를 보이지 않고 일정한 수준으로 유지되는 것으로 나타났으나 생후 9주에는 MO군에서 SO군보다 높게 나타났다. 실험식이내 oleic ac:인 조성은 SO군이 MO군보다 매우 낮으나, SO군의 모유에서 oleic acid 조성비율이 식이에 비해 크게 증가된 것으로 나타나, 모유가 뇌 성장$.$발달기간중의 뇌조직내 oleic acid의 주요 급원이 될 수 있음을 나타내주고 있다. 18:0에서 oleic acid의 de novo 합성 정도를 나타내는 간접지표인 -9 desaturation index는 생후 3주에는 실험군간(시냅토솜 예외)에 유의한 차이를 보이지 않았으나, 생후 9주에는 식이내 oleic acid가 풍부한 MO군의 시냅토솜에서 높게 나타났다. 한편 생후 9주 마이크로솜 분획 에서 olelc acid 수준은 실험군 간에 차이를 보이지 않았으나, -9 desaturation index는 SO군에서 MO군보다 높아 뇌조직 에서 oleic acid가 생합성될 수 있음을 간접적으로 설명 해주고 있다. 따라서 흰쥐 뇌조직의 oleic acid는 식이와 모유 등의 이미 합성된 oleic acid가 뇌로 우선적으로 유입되는 부분과 뇌조직 자체에서 합성되는 부분에 의한다고 생각된다. 그러나 식이중 oleic acid가 부족하면 뇌 세포분획내의 oleic acid수준이 유의하게 감소하였으므로, 식이중 적절한 수준의 oleic acid가 정상적인 뇌발달에 필수적임을 다시 한번 강조하게 한다.