• Food Science of Animal Resources
• /
• v.41 no.4
• /
• pp.623-635
• /
• 2021

The effect of deer antler extract on muscle differentiation and muscle atrophy were evaluated to minimize muscle loss following aging. Various deer antler extracts (HWE, hot water extract of deer antler; FE, HWE of fermented deer antler; ET, enzyme-assisted extract of deer antler; UE, extract prepared by ultrasonication of deer antler) were evaluated for their effect on muscle differentiation and inhibition of 5-aminoimidazole-4-carboxamide ribonucleoside (AICAR)-induced muscle atrophy in C2C12 cells. Morphological changes according to the effect of antler extracts on muscle differentiation were confirmed by Jenner-Giemsa staining. In addition, the expression levels of genes related to muscle differentiation and atrophy were confirmed through qRT-PCR. In the presence of antler extracts, the length and thickness of myotubes and myogenin differentiation 1 (MyoD1) and myogenic factor 5 (Myf5) gene expression were increased compared to those in the control group (CON). Gene expression of AMP-activated protein kinase (AMPK), MyoD1, and myogenin, along with the muscle atrophy factors muscle RING finger-1 (MuRF-1) and forkhead box O3a (FoxO3a) upon addition of deer antler extracts to muscle-atrophied C2C12 cells was determined by qRT-PCR after treatment with AICAR. The expression of MuRF-1 and FoxO3a decreased in the groups treated with antler extracts compared to that in the group treated with AICAR alone. In addition, gene expression of MyoD1 and myogenin in the muscle atrophy cell model was significantly increased compared that into the CON. Therefore, our findings indicate that antler extract can increase the expression of MyoD1, Myf5 and myogenin, inhibit muscle atrophy, and promote muscle differentiation.

• Journal of the Korean GEO-environmental Society
• /
• v.12 no.7
• /
• pp.13-21
• /
• 2011

In seasonal frozen areas with climatic features, which have a temperature difference in the winter and thawing season, changes of mechanical properties of the soil in the zone could be seen between the freezing and thawing surface. In particular, in soil with many fine particles, a softening of the roadbed usually occurs from frost and thawing actions. The lower bearing capacity is a rapidly progressive the softening of roadbed, and occurred a mud-pumping by repeated loading. In this study, the three kind of sandy soil with contents of fine particles were conducted by directly shear box test with the number of cyclic in freeze-thawing and the water content of soil. Subsequently, the relationship between the shear strength and freeze-thaw cycling time was obtained. The shear strength was decreased with the increase of the freeze-thaw cycling time. A shear stress deterioration of the soil with power function modal is proposal.

• Journal of Korean Society of Forest Science
• /
• v.100 no.3
• /
• pp.397-401
• /
• 2011

This study was conducted to clarify the breeding ecology of tits Parus spp. used artificial nest boxes in a deciduous forest at the Ansung Campus, Chung-Ang University from March through July, 2006 to 2010. Varied tit Parus varius, marsh tit P. palustris and great tit P. major bred in artificial nest boxes during our study period. There were no differences in number of breeding bird pairs among different entrance diameters of artificial nest boxes. The number of breeding pairs of great tits was the most highest among tits and that of varied tits was the least. Great tits mainly used 40 mm and 35 mm artificial nest box entrance diameter and marsh tits preferred 30 mm entrance diameter. In three tits species, there were differences in first egg date, clutch size, brood size, egg incubation period, nestling care period and breeding success among study years. It is needed the study on long-term ecological research about breeding ecology of tits related with habitat conditions.

• Entomological Research
• /
• v.48 no.5
• /
• pp.390-399
• /
• 2018

In order to precisely assess gene expression levels, the suitable internal reference genes must be served to quantify real-time reverse transcription polymerase chain reaction (RT-qPCR) data. For armyworm, Mythimna separata, which reference genes are suitable for assessing the level of transcriptional expression of target genes have yet to be explored. In this study, eight common reference genes, including ${\beta}$-actin (${\beta}$-ACT), 18 s ribosomal (18S), 28S ribosomal (28S), glyceraldehyde-3-phosphate (GAPDH), elongation fator-alpha ($EF1{\alpha}$), TATA box binding protein (TBP), ribosomal protein L7 (RPL7), and alpha-tubulin (${\alpha}$-TUB) that in different developmental stages, tissues and insecticide treatments of M. separata were evaluated. To further explore whether these genes were suitable to serve as endogenous controls, three software-based approaches (geNorm, BestKeeper, and NormFinder), the delta Ct method, and one web-based comprehensive tool (RefFinder) were employed to analyze and rank the tested genes. The optimal number of reference genes was determined using the geNorm program, and the suitability of particular reference genes was empirically validated according to normalized HSP70, and MsepCYP321A10 gene expression data. We found that the most suitable reference genes for the different experimental conditions. For developmental stages, 28S/RPL7 were the optimal reference genes, both $RPL7/EF1{\alpha}$ were suitable for experiments of different tissues, whereas for insecticide treatments, $28S/{\alpha}-TUB$ were suitable for normalizations of expression data. In addition, $28S/{\alpha}-TUB$ were the suitable reference genes because they have the most stable expression among different developmental stages, tissues and insecticide treatments. Our work is the first report on reference gene selection in M. separata, and might serve as a precedent for future gene expression studies.

• Journal of English Language & Literature
• /
• v.57 no.4
• /
• pp.625-649
• /
• 2011

Even though "An Outpost of Progress" and Heart of Darkness were based upon Joseph Conrad the sailor's same experience in Congo Free State, their narrative strategies are quite different. The realistic representation of "An Outpost of Progress," with which Conrad was not satisfied at all, was converted into the modernistic narrative strategy of Heart of Darkness so that the sympathetic power of the story should be improved. The conservative value system of realism is expressed by the omniscient author in "An Outpost of Progress," whereas the frame narrator of Heart of Darkness is proved to be an unreliable one whose norms and behavior are not in accordance with the implied author. The glorious history of the British Empire, which was proudly presented by the frame narrator at the beginning of Heart of Darkness, was strongly opposed by Marlow, another narrator, who said that the British Empire had been "one of the dark places of the earth" when ruled by the Roman Empire. The feeling of the frame narrator was uneasily changed into the gloomy mood when he described the Thames as the flow which "seemed to lead into the heart of an immense darkness" at the end of Heart of Darkness. Similar to the straightforward narrative strategy of representation in "An Outpost of Progress," the realistic approach of Part I in Heart of Darkness is considered by Conrad as insufficient to reveal the darkest truth of imperialism, which was declared by Kurtz as "The Horror! The Horror!" Thus Conrad uses the Chinese-box structure, in which Kurtz' episode is enveloped by Marlow's tale which is enclosed by the frame narrator's story, in order to penetrate into the mind of ordinary readers in the novelist's age of New Colonialism, while attacking the ideology itself of imperialism instead of critisizing its inefficiency and individualism.

• Korean Journal of Metals and Materials
• /
• v.49 no.1
• /
• pp.25-31
• /
• 2011

Solid oxide fuel cells (SOFCs) have many attractive features for widespread applications in generation systems. Recently, stainless steels have attractive materials for metallic bipolar plate because metallic bipolar plates have many benefits compared to others such as graphite and composite bipolar plates. SOFC operates on high temperature of about $800{\sim}1000^{\circ}C$ than other fuel cell systems. Thus, many studies have attempted to reduced the operation temperature of SOFC to about $600{\sim}800^{\circ}C$, which is the intermediate temperature (IT) of SOFC. Low cost and high-temperature corrosion resistance are very important for the practical applications of SOFC in various industries. In this study, two specimens, 304 and 430 stainless steels with and without different pre-surface treatments on the surface were investigated. And, specimens were exposed at high temperature in the box furnace under oxidation atmosphere of $800^{\circ}C$. Oxidation behavior have been investigated with the materials exposed at different times (100 hrs and 400 hrs) by SEM, EDS and XRD. By increasing exposure time, the amount of metal oxide increased in the order like; STS304 < STS430 and As-received < As-polished < Sand-blast specimens.

• Journal of the Korea Institute of Information Security & Cryptology
• /
• v.21 no.5
• /
• pp.47-58
• /
• 2011

Recently, there are several technologies for protecting information in the lightweight device, One of them, the AES[1] algorithm and CRT mode, is used for numerous services(e,g, OMA DRM, VoIP, IPTV) as encryption technique for preserving confidentiality. Although it is possible that the AES algorithm CRT mode can parallel process transmitting data, IPTV Set-top Box or Mobile Device that uses these streaming service has limited computation-ability. So optimizing crypto algorithm and enhancing its efficiency for those environment have become an important issue. In this paper, we propose implementation method that can improve efficiency of the AES-CRT Mode by improving algorithm logics. Moreover, we prove the performance of our proposal on the mobile device which has limited capability.

• Journal of the Korea Institute of Information Security & Cryptology
• /
• v.20 no.3
• /
• pp.53-65
• /
• 2010

Conditional Access System (CAS) is a core security mechanism of IPTV SCP (Service and Content Protection) which enables only authenticated user to be able to watch the broadcasting contents. In the past, it was general that CAS was built in Set-Top Box (STB) as hardware or as a detachable cable card. However, numerous researches in Downloadable CAS (DCAS), where users can download CAS code in their STB through their network, have been recently conducted widely due to the lack of security and scalability problem. In this paper, the security requirements of OpenCable based DCAS which is typical example of downloadable IPTV SCP will be derived, the novel authentication and key management scheme will be proposed by using the Authentication Proxy (AP) which is the core DCAS. Also, the benefits of the proposed system will be evaluated by comparison and analysis with preceding research.

• Journal of the Korea Institute of Information Security & Cryptology
• /
• v.20 no.3
• /
• pp.67-78
• /
• 2010

CAS(Conditional Access System) is used in Pay-TV System to prohibit unauthorized user(s) accessing the contents in IPTV broadcasting environment. In the CAS, Smartcard transfers CW which is necessary in the process of descrambling the scrambled program to STB. CW hacking problem is one of the most serious problems in pay-TV system. There have been many researches on generating secure communication channel between smartcard and STB for secure transmitting, But they had problems in efficiency and security. In this paper, we propose a lightweight key agreement protocol based on a symmetric key algorithm. We show that our proposed protocol is more efficient than existing protocols by comparing the amount of computations, and analyzing the security requirement of the proposed protocol.

• Journal of the Korea Institute of Information Security & Cryptology
• /
• v.21 no.2
• /
• pp.13-25
• /
• 2011

Biometric-based authentication can provide strong security guarantee about the identity of users. However, security of biometric data is particularly important as the compromise of the data will be permanent. The fuzzy fingerprint vault system is one of the most popular solutions for protecting the fingerprint template stored in the database. Recently, however, this system is very susceptible to a correlation attack that finds the real minutiae using multiple fingerprint vaults enrolled for different applications. To solve this problem, we propose a robust fuzzy fingerprint vault system against the correlation attack. In this paper, we add chaff minutiae based on the relative information of minutiae such as direction, coordinate instead of adding randomly. Also, our proposed approach allow to add multiple chaff minutiae within tolerance box for enhanced security level. Experimental results show that the proposed approach can protect the correlation attack and achieve enhanced verification accuracy.