• 한국수정란이식학회지
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• 제14권3호
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• pp.211-217
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• 1999

The objective of this study was to determine the cause and prevalence of bovine abortion and stillbirth in Kyungi-do area. Seventy three bovine fetuses were collected from farms and submitted to the College of Veterinary Medicine, Seoul National University. Submitted fetuses were evaluated during a 4-month period (July to November, 1999) for pathological lesion, tissue protozoa, bacteria and viral infection. The average proportion of abortions was decreased with parity in 73 abortion heifers and cows. Monthly incidence rate of bovine abortion was not different in this study. In fetuses from 90 to 282 days gestation, the majority were between 150 and 250 days gestation(58%). The cause of abortion or stillbirth was determined in 51% of the cases examined. In 15(21%) of the fetus, neosporosis were diagnosed by pathological findings. In three (4%) additional fetuses in three additional fetuses, suspected Neosporosis by pathological lesion, and in 3 (4%) fetuses examined Neopsorosis were diagnosed in 15 feturses and in 3 fetuses, Neosporosis was suspected by pathological legions. Neosporosis / viral infection were diagnosed in three additional fetuses). Miscellaneous bacterial infection, BVDV, iatrogenic cause, Neosporosis / IBRV / BVDV, miscellaneous viral, IBRV/BVDV and others were 3(4%), 3(4%), 2(3%), 2(3%), 1(1%), 1(%) and 9(12%) respectively. The cause and incidence of bovine abortion in different area in Kyungi-do was not different in this study.

• 한국수정란이식학회지
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• 제17권2호
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• pp.163-168
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• 2002

1999년 6월부터 2002년 4월까지 총 2년 11개월 간 전국에서 발생한 소 유조사산에 대한 임상학적 연구결과는 다음과 같았다. 1. 계절별 소 유조사산 발생은 여름에 다른 계절에 비해 많이 발생하였다. 이는 여름철의 고온 다습한 기후와 낮은 우군관리 집중도에 기인한다고 사료된다. 2. 임신우의 산차별 유조사산 발생 비율을 조사한 결과 산차가 낮은 소에서 많이 발생하였다. 이는 유조사산의 원인이 임신우의 산차수에 있기보다는 산차수가 높은경우 사육되는 임신우의 절대수가 적기 때문이다. 3. 임신기간별 유산 발생을 조사한 결과 임신 150∼250일 및 임신 251일 이후에 집중 발생하였다. 이는 임신 초기보다 유산태아의 발견이 용이하고, 유산이 주로 임신 중기에 다발하는 Neospora caninum으로 진단된 예가 많았기 때문인 것으로 사료된다. 4. 유산이 발생한 농장에서의 vaccination 여부를 조사한 결과 총 75％의 목장에서 백신접종을 하지 않은 것으로 조사되었다. 이는 임신우가 유산원인체에 접촉되었을 때 쉽게 감염될 수 있는 위험성이 높은 것으로 사료된다. 5. 조사당시 확인된 180개 목장 중 36개 목장에서 동가가축을 사육하였다. 또한 Neospora caninum으로 진단된 22개 목장 중 6개 목장에서 개를 사육하고 있어 동거가축에 의해 소에서 유산이 발생의 연관성은 더 깊은 연구가 필요할 것으로 사료된다. 6. 유조사산 후 생식기 검사는 모체의예후 판정 및 생식기 수복을 위한 적절한 임상학적 처치를 위한 지표로 이용할 수 있었다.

• 대한수의학회지
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• 제41권3호
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• pp.381-385
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• 2001

Neospora caninum infections have been associated with neonatal paresis as well as abortion around the world. Bovine abortion induced by N caninum was first reported in 1997 in Korea. Diagnosis of N caninum infection is usually based on histopathology and immunohistochemical detection of organism. However, often the tissues having lesion suggestive of N caninum infection were negative on immunohistochemistry. Here, we describe establishment of PCR-based diagnostic strategy for N caninum infection using DNA extracted from paraffin blocks containing the lesion. PCR was able to amplify N caninum-specific bands from the paraffin blocks containing at least moderate degree of inflammation. Compared to paraffin-blocks, DNA extracted from fresh tissues were less sensitive than that of paraffin blocks. This PCR-based method can be practically applicable for rapid diagnosis of bovine N caninum infection with high specificity and sensitivity. Based on this method, 17% of bovine abortion surveyed during a designated period was associated with N caninum infection.

• 대한수의학회지
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• 제38권4호
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• pp.853-858
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• 1998

A case of repeated bovine abortion caused by Neospora caninum is described. The 3-year-old Holstein cow, which was seropositive to N caninum antibody by indirect immunofluoresent assay, aborted consecutively within a 10-month period. Two fetuses of 6- or 5-month of gestation were aborted in June 1996 and March 1997, respectively. Histologically, multifocal necrotizing encephalitis, nonsuppurative periportal hepatitis, myocarditis, and myositis were observed in both fetuses. Tachyzoites or tissue cysts detected in the brain, heart and liver were positive to N caninum but negative to Toxoplasma gondii by immunohistochemical method. The results of our study demonstrate that repeated abortion due to N caninum actually occurs in Korea. This is believed to the first report of repeated abortion associated with N caninum in Korea.

• 한국동물위생학회지
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• 제37권3호
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• pp.179-183
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• 2014

Reproductive losses in a herd can be huge. Fetal reabsorption or undefined infertility often remain undetected. Routine herds monitoring for exposure, controlling the introduction of potential agent carriers, appropriate biosafety procedures, and vaccination where possible are together the best security against abortion and stillbirth inducing disease. For biosecurity of local farms, we performed antibody titers of abortion and stillbirth related diseases such as bovine viral diarrhea virus (BVDV), infectious bovine rhinotracheitis virus (IBRV), Neospora caninum, Toxoplasma gondii and Campylobacter fetus subsp venerealis. The blood samples were collected from 500 female Hanwoo over 1 year old of 100 farms in Jeonbuk eastern area. Champhylobater serological test was evaluated by the standard tube agglutination test (STAT) and other pathogen's antibodies were detected by indirect-enzyme linked immunoassay (I-ELISA). The seroprevalence of abortion and stillbirth inducing disease were BVDV 72.4%, IBRV 13.0%, N. caninum 1.2%, T. gondii 10.4% and C. venerealis 0.6%, irrespectively.

• 한국수정란이식학회지
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• 제25권1호
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• pp.43-50
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• 2010

The specimens from 32 aborted fetus and 274 aborted cows were collected in 168 farms of Chonnam province from 2005 to 2008 and were tested the brucellosis. The results obtained are summarized as follows. In the 32 aborted fetus, bovine brucellosis was detected in 12 heads (37.5%), bovine viral diarrhea-mucosal disease was detected in 7 heads (19%), ainovirus infection was detected in 1 head (3.1%), and multi-infection of BVD and brucellosis was not detected, respectively. In the 306 cases of aborted fetus and cows, bovine brucellosis was detected in 44 heads (14%). Status of abortion were confirmed in 63 farms (38%) out of 168 farms from June to August. From the point of raising scale, studies found that 128 farms (76%) out of all raised under 20 heads. The incidence of abortion by brucellosis was mainly showed in 30 heads (68.1%) about 151~250 days of gestation. In the result of the 18 farms survey, the causes of infections were detected movement of infected cattle in 5 farms (28%), unknown cause in 12 farms (67%), and recurrence in 1 farm (5%). The results of this study suggest to take an advantage of the prevention and fundamental research for bovine brucellosis in Chonnam province.

• 한국가축번식학회지
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• 제1권1호
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• pp.29-33
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• 1977

• 대한수의사회지
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• 제16권1112호
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• pp.355-362
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• 1980

The purposes of this study were to determine the incidence of the abortion and vaginitis due to Aspergilli and to research the Aspergilli in the vaginal exudates from apparently healthy dairy cows in the Gyeongbug area. Fetuses from 13 aborted cows, vagin

• 대한수의학회지
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• 제37권3호
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• pp.607-612
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• 1997

A case of neosporosis is described in a 6-month-old aborted bovine fetus. Grossly, numerous, well-demarcated, 2-3mm, yellowish white foci were scattered in the heart and skeletal muscle. Histopathologic changes were confined to the brain, heart, and skeletal muscle. Multifocal areas of necrotizing encephalitis were observed in the cerebrum as well as cerebellum. Severe multifocal to coalescing nonsuppurative inflammation was observed in the myocardium and skeletal muscle. The tachyzoites of Neospora caninum were demonstrated in the brain, myocardium and skeletal muscle with immunohistochemical method using Neospora caninum-specific antibody. Based on the histopathology and immunohistochemistry, this case was diagnosed as neosporosis in aborted Korean cattle. This is believed to be the first reported case of bovine neosporosis in Korea.

• 대한수의학회지
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• 제38권4호
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• pp.859-866
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• 1998

This study was carried out to investigate the prevalence of Neopsora (N) caninum infection in Korean dairy herds. To determine the prevalence of antibodies to N caninum in Korean dairy cattle, a total of 1,688 sera including 895 sera taken from 30 herds having recent high abortion rate and 793 sera selected randomly from 168 herds with no history of recent abortion problem, respectively, collected nationwide during a designated period were analyzed by indirect fluorescent antibody (IFA) test. Mean nationwide seropositive rate of the sera tested in herds and individual cattle tested were 53.5% and 35.6%, respectively. However mean seropositive rate of the samples from herds having abortion problem was approximately two and half times higher than those in herds with no recent abortion history. Regional seropositive rates of the samples from the herds with abortion problem were 48.6%, 51.6%, 44.4% and 71.4% at Kyunggi, Kangwon, Kyungbuk and Jeonnam province, respetively. Regional seropositive rates of the samples from the herds with no recent abortion problem were 35.6%, 18.3%, 16.5%, 37.5%, 19.4%, 33.3%, 32.1%, 3.8% and 0.0% at Kyunggi, Kangwon, Chungbuk, Chungnam, Kyungbuk, Kyungnam, Jeonbuk, Jeonnam and Jeju province, respectively. The results of this study suggested that N caninum infection was widespread and considerably associated with bovine abortion in Korea.