• Journal of Microbiology and Biotechnology
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• v.21 no.5
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• pp.525-528
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• 2011

The Raf-1 kinase inhibitory protein (RKIP) can regulate multiple key signaling pathways. Specifically, RKIP binds to Raf-1 kinase and inhibits the Ras-Raf-1-MEK1/2- ERK1/2 pathway. Additionally, Raf-1 has been shown to translocate to mitochondria and thereby protect cells from stress-mediated apoptosis. Recently, HBx was found to stimulate the mitochondrial translocation of Raf-1, contributing to the anti-apoptotic effect. We found that RKIP was downregulated during HBx-mediated hepatocarcinogenesis. In this study, we show that RKIP bound to Raf-1 and consequently inhibited the translocation of Raf-1 into mitochondria. This promoted the apoptosis of cells treated with apoptotic stimulus. Thus, the downregulation of RKIP increased the level of free Raf-1 and thereby elevated the mitochondrial translocation of Raf-1 during HBx-mediated hepatocarcinogenesis. The elevated Raf-1 mitochondrial translocation induced the increased anti-apoptotic effect and subsequently promoted HBx-mediated hepatocarcinogenesis.

• Korean Journal of Veterinary Research
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• v.48 no.3
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• pp.243-250
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• 2008

A calmodulin-dihydrofolate reductase (DHFR) sandwich fusion protein was generated by insertion of calmodulin into the $\beta$-bulge region of DHFR to observe the effects of structurally constraining the calmodulin structure. The calcium binding properties of the sandwich protein were almost identical to calmodulin. Similar to calmodulin ($10.7 {\mu}M$), the sandwich protein bound four equivalents of calcium, with half saturation ($K_{0.5}$) observed at a [$Ca^{2+}$] of $8{\mu}M$. However, nicotinamide adenine dinucleotide (NAD) kinase activation property of the sandwich protein was lower than that of calmodulin. The sandwich protein activated NAD kinase, but to only half of the level obtained with calmodulin. The K 0.5 for both calmodulin and the sandwich protein were approximately the same (1-2 nM). Methylation analyses of the sandwich protein show that insertion of calmodulin into DHFR results in a large decrease in methylation. The $V_{max}$ observed with the sandwich protein (95 nmole/min/ml) was only 22% of the value observed with calmodulin (436 nmol/min/ml) in the presence of calcium. Addition of trimethoprim to the reaction significantly inhibited the observed methylation rate. Overall, the data suggest that the insertion of calmodulin into the DHFR structure has little effect on calcium binding by the individual lobes of calmodulin, but may constrain the lobes in a manner that results in altered interaction with the calmodulin-dependent proteins, and severely perturbed the methyltransferase recognition site.

• Korean Journal of Optics and Photonics
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• v.10 no.5
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• pp.405-412
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• 1999

We propose an effective algorithm, which can predict the detailed behavior of the intensity-modulated high speed optical signal after propagating through an optical fiber. The alogrithm is based on the SSF (Split Step Fourier) Method, however, the step size is automatically calibrated in each calculation step to reduce the number of calculations within given round-off error bound. Applying the algorithm to the 2.5 Gbps 100 km transmission and 10 Gbps 40 km transmission simulations, we achieved the calculation time reduction by maximum 1/120 and 1/56 of the calculation time by using the SSF fixed step algorithm previously known. The root-mean-square of the round-off error was kept within -30 dB compared to the signal level throughout the calculation.

• Animal cells and systems
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• v.11 no.2
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• pp.169-173
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• 2007

Hyperpolarization-activated nonselective cation channels (HCNs) play a pivotal role in producing rhythmic electrical activity in the heart and the nerve cells. In our previous experiments, voltage-dependent $Cd^{2+}$ access to one of the substituted cysteines in S6, T464C, supports the existence of an intracellular voltage-dependent activation gate. Direct binding of intracellular cAMP to HCN channels also modulates gating. Here we attempted to locate the cAMP-modulated structure that can modify the gating of HCN channels. SpHCN channels, a sea urchin homologue of the HCN family, became inactivated rapidly and intracellular cAMP removed this inactivation, resulting in about eight-fold increase of steady-state current level. T464C was probed with $Cd^{2+}$ applied to the intracellular side of the channel. We found that access of $Cd^{2+}$ to T464C was strongly gated by cAMP as well as voltage. Release of bound $Cd^{2+}$ by DMPS was also gated in a cAMP-dependent manner. Our results suggest the existence of an intracellular cAMP-modulated gate in the lower S6 region of spHCN channels.

• Bulletin of the Korean Chemical Society
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• v.28 no.12
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• pp.2454-2458
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• 2007

Exploring the basic concepts for the design of CO2-philic molecules is important due to the possibility for “green” chemistry in supercritical CO2 as substitute solvent systems. The Lewis acid-base interactions and C?H…O weak hydrogen bonding were suggested as two key factors for the solubility of CO2-philic molecules. We have performed high level quantum mechanical calculations for the van der Waals complexes of CO2 with trimethylphosphate and trimethylphosphine oxide, which have long been used for metal extractants in supercritical CO2 fluid. Structures and energies were calculated using the MP2/6-31+G(d) and recently developed multilevel methods. These studies indicate that the Lewis acid-base interactions have larger impact on the stability of structure than the C?H…O weak hydrogen bonding. The weak hydrogen bonds in trimethylphosphine oxide have an important role to the large supercritical CO2 solubility when a metal is bound to the oxygen atom of the P=O group. Trimethylphosphate has many Lewis acid-base interaction sites so that it can be dissolved into supercritical CO2 easily even when it has metal ion on the oxygen atom of the P=O group, which is indispensable for a good extractant.

• Journal of Environmental Science International
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• v.12 no.8
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• pp.835-840
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• 2003

Many various kinds of tourism resources have been distributed along the Nakdong river basin. Previous researches have focused mainly on environmental studies, including water quality control, industrial use of water, biodiversity, etc, with little research done in the field of tourism studies. Central to this study, therefore, is the identification and analysis, from the perspective of cultural tourism, of the area's distribution and characteristics of cultural properties registered by the MOCT. Review of related literature reveals : 1) spatial range bound with the jurisdiction of the river basin like DREO and NRBEO ; 2) analytical subjects limited to cultural properties designated by the three different administrative units of government, city and province. Along with the DREO's predominance over the NRBEO in the total number of cultural properties, the result finds that two cities, Andong and Gyeongju of Gyeongbuk are assigned ownership of most of the cultural properties under the jurisdiction of DREO, while Gyeongnam that of most of the ones under the NRBEO. However, those findings suggest the simple number of cultural properties with no significant level of importance and rarity value reflected. Therefore, future studies need to develop quantified modelling keeping cultural variables in mind and create cultural indices of the competitiveness of the local governments.

• Journal of Positioning, Navigation, and Timing
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• v.7 no.1
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• pp.25-35
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• 2018

This paper describes the measurement of pulse positioning (input time) to calculate a time of arrival (TOA) that takes from transmitting a signal from the target of multilateration (MLAT) system to receiving the signal at the receiver. In this regard, this paper analyzes performances of simple threshold method and level adjust system (LAS) method, which is one of the adaptive threshold detector (ATD) methods, among many methods to calculate pulse positioning of signal received at the receiver. To this end, Cramer-rao lower bound (CRLB) with regard to pulse positioning, which was measured when signals transmitted from a transponder mounted at the target were received at the receiver, was induced and then deviation sizes with regard to pulse positioning, which was measured with simple threshold and LAS methods through MATLAB simulations, were compared. Next, problems occurring according to a difference in amplitude of signals inputted to each receiver are described when pulse positioning is measured at multiple receivers located at a different distance from the target as is the case in the MLAT system. Furthermore, LAS method to resolve the problems is explained. Lastly, this study analyzes whether a pulse positioning error occurring due to the signal noise satisfies the requirement (6 nsec. or lower) recommended for the MLAT system when using these two methods.

• Korean Journal of Agricultural Science
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• v.43 no.2
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• pp.275-287
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• 2016

This study attempts to estimate the benefits of using water ensuing from the Chilgap multipurpose reservoir for environmental improvement. The contingent valuation method (CVM) was used to elicit the willingness to pay (WTP) for providing environmental improvement water from the Chilgap reservoir to Daechi-stream and Ji-stream. The DCDB (double-bound dichotomous choice) survey method was used to collect data for the analysis. Due to the usually high cost of increasing the sample size, the use of follow-up questions was implemented as an inexpensive method of improving the efficiency of the estimation. A spike model was used in this study because a number of respondents showed zero WTP. The spike model can be estimated as easily as the conventional model. Results show that the average annual household's WTP is 4,516 won using the conventional model and 8,644 won using the spike model. Applying the estimated average annual household's WTP to the Chungnam and Daejeon regional levels, the benefits of environmental improvement water from the Chilgap reservoir is estimated at 11.9 billion won per year. The temporal benefits of providing water for environmental improvement, for a 50-year period at a 6.0% discount rate, is estimated at about 190 billion won in the Chungnam and Daejeon areas. These results could be useful especially when the government tries to determine an appropriate level of investment and to make a policy related to providing environmental improvement water.

• Aerospace Engineering and Technology
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• v.8 no.2
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• pp.92-97
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• 2009

Two conventional speed detection methods (Elapsed-time method and Pulse-count method) are analyzed and compared for a high speed motor with digital tacho pulse with non-uniformity. In general, the elapsed-time method usually has better performance than a pulse-count method in case sufficiently high speed clock is used to measure the time difference. But if a tacho pulse non-uniformity exists in the reaction wheel - most of reaction wheel has a certain amount of non-uniformity - the accuracy of the elapsed-time method is degraded significantly. Thus the performance degradation is analyzed with respect to the level of non-uniformity of tacho pulse distribution and an allowable bound is suggested.

• Earthquakes and Structures
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• v.18 no.1
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• pp.45-56
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• 2020

The application of critical excitation method with displacement-based objective function for multi degree of freedom (MDOF) systems is investigated. To this end, a new critical excitation method is developed to find the critical input motion of a MDOF system as a synthetic accelerogram. The upper bound of earthquake input energy per unit mass is considered as a new constraint for the problem, and its advantages are discussed. Considering this constraint, the critical excitation method is then used to generate synthetic accelerograms for MDOF models corresponding to three shear buildings of 10, 16, and 22 stories. In order to demonstrate the reliability of generated accelerograms to estimate dynamic response of the structures, three target ground motions with considerable level of energy contents are selected to represent "real critical excitation" of each model, and the method is used to re-generate these ground motions. Afterwards, linear dynamic analyses are conducted using these accelerograms along with the generated critical excitations, to investigate the key parameters of response including maximum displacement, maximum interstory drift, and maximum absolute acceleration of stories. The results show that the generated critical excitations can make an acceptable estimate of the structural behavior compared to the target ground motions. Therefore, the method can be reliably implemented to generate critical excitation of the structure when real one is not available.