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Studies on Lipids in Fresh-Water Fishes 1. Distribution of Lipid Components in Various Tissues of Crucian Carp, Carassius carassius (담수어의 지질에 관한 연구 1. 붕어(Carassius carassius)의 부위별 지질성분의 분포)

  • CHOI Jin-Ho;RO Jae-Il;PYEUN Jae-Hyeong;CHOI Kang-Ju
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.17 no.4
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    • pp.333-343
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    • 1984
  • This study was designed to elucidate the lipid and its fatty acid composition in various tissues of fresh water fishes. The free and bound lipids in meat, skin and viscera of crucian carp (Carassius carassius) were extracted with ethyl ether and the mixed solvent of chloroform-methanol-water (10/9/1, v/v). The free and bound lipids were fractionated into neutral lipid, glycolipid and phospholipid by a silicic acid column chromatography using chloroform, acetone and methanol, respectively, and quantitatively analyzed by thin layer chromatography (TLC) and TLC scanner. The fatty acid compositions of polar ana nonpolar lipids in meat, and these of neutral lipid in various tissues were analyzed by gas liquid chromatography(GLC). The free lipid content in meat, skin and viscera was $6.22\%,\;9.95\%\;and\;9.76\%$, whereas the bound lipid content in those tissues was $10.01\%,\;3.56\%\;and\;7.36\%$, respectively. The neutral lipid contents in free lipid were ranged from $71.7\%$ to $89.4\%$, and $3{\sim}9$ times higher than those in bound lipid, while the phospholipid contents in bound lipid were ranged from $42.3\%$ to $63.2\%$, and $5{\sim}10$ times higher than those in free lipid. The neutral lipid was mainly consisted of triglyceride ($81.91{\sim}88.34\%$) in free lipid, and esterified sterol & hydrocarbon ($41.00{\sim}59.43\%$) in bound lipid. The phospholipid was mainly consisted of phosphatidyl ethanolamine($54.56{\sim}66.79\%$) and phosphatidyl choline ($21.88{\sim}34.28\%$) in free lipid, and phosphatidyl choline ($50.49{\sim}70.57\%$) and phosphatidyl ethanolamine ($15.74{\sim}24.92\%$) in bound lipid. The major fatty acids of polar lipid in free and bound lipids were $C_{16:0}\;(17.53\%,\;19.29\%)$, $C_{18:1}\;(24.57\%,\;16.08\%)$, $C_{18:2}\;(8.39\%,\;4.03\%)$, $C_{22:5}\;(1.68\%,\;8.08\%)$, and $C_{22:6}\;(6.22\%,\;13.60\%)$ and these of neutral lipid in free and bound lipids were $C_{16:0}\;(17.67\%,\;24.15\%)$, $C_{16:1}\;(12.81\%,\;5.52\%)$, $C_{18:1}\;(24.13\%,\;13.02\%)$, $C_{18:2}\;(15.47\%,\;8.68\%)$, $C_{22:5}\;(0.88\%,\;4.14\%)$ and $C_{22:6}\;(1.17\%,\;5.04\%)$, respectively. The unsaturations (TUFA/TSFA) of polar lipid in free and bound lipids were 2.02 and 2.74, and $1.5{\sim}2.0$ times higher than 1.51 and 1.23 of nonpolar lipid. In both polar and nonpolar lipids, w3 highly unsaturated fatty acid (w3HUFA) content of bound lipid was $2{\sim}5$ times higher than that of free lipid. The polyenoic acid contents such as $C_{20:5},\;C_{22:5}\;and\;C_{22:6}$ in bound lipid were $2{\sim}5$ times higher than these in free lipid. Consequently, there were significant difference between the lipid and its fatty acid composition in free and bound lipids and/or in various tissues.

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Substantial Estimated Availability and NDF-Bound Trace Minerals (Fe, Zn, Cu, and Mn) Contents of Seven Different Seaweeds (7종 해조류 NDF에 결합된 미량무기질(Fe, Zn, Cu, Mn) 함량 및 추정 이용률)

  • Kwak, Yeon-Hwa;Kim, Hyeong-Soo;Choi, Su-Jin;Kim, Dae-Jin
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.39 no.7
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    • pp.1078-1082
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    • 2010
  • The substantial trace mineral usabilities of seven seaweeds for nutrient evaluation have been studied. As the neutral detergent fiber (NDF) bound trace minerals have been known to be indigestible, these contents were excluded in the digestible trace mineral contents evaluation. Therefore, those four trace mineral contents in seven seaweeds and their NDF-bound contents were estimated. Consequently, the NDF-bound Zn, Cu and Mn contents, except Fe, in seven seaweeds were between 10 and 20% of total trace mineral contents, thus 80~90% of seaweeds trace minerals were observed to be substantially digestible. Although the average estimated availability of Fe in the seaweeds were relatively low, those Zn, Cu and Mn were high in relative to those total contents. Thus, even with consideration of their digestibility, seaweeds could be one of good sources of trace minerals for human nutrition because of their high availabilities of Zn, Cu and Mn.

Changes in the Fatty Acid Composition of Phospholipid in the Dried and Salted Mullet Roe during Processing and Storaging (염건숭어알의 가공과 저장중 인지질의 지방산 함량 변화)

  • Joe, Sang-June;Jo, Jung-Hwan
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.22 no.3
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    • pp.286-290
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    • 1993
  • Mullet roe was salted and dried by the conventional processing method. Mullet roe was first salted with soybean sauce containing 10% NaCl and then pressed down to be a 1.2cm of thickness. It was dried at 2$0^{\circ}C$ under 3m/sec of aeration for 20 days. The lipids of the processed roe were fractionated by free and bound phospholipids. The contents of free and bound phospholipids were 9.30mg/100mg and 13.0mg/100mg respectively. The content of bound lipids were rapidly decreased than that of free fatty acids during processing and storaging. The major fatty acids of phospholipids were $C_{l6:0}$, $C_{20:0}$, $C_{16:1}$, $C_{l8:2}$ and $C_{20:5}$ whose contents were 6.64mg/100mg that occupied 72% of the total phospholipids. The ratio for the unsaturated fatty acids to the saturated ones of free phospholipids in fresh roe was 1.53 and it was decreased down to 0.34 in 9 weeks of storage. But the ratio of bound phospholipids was 1.04 of fresh roe and zero in 6 weeks. The content of essential fatty acids in bound phospholipids was 3.85mg/100mg occupying 75% of total essential fatty acids of the fresh roe, but they were totally destroyed during processing.g.g.g.

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Lipid and Fatty Acid Compositions of Korean Corns (한국산(韓國産) 옥수수의 지질성분(脂質成分)에 관(關)한 연구(硏究))

  • Chun, Ho-Nam;Choi, Kap-Seong;Kim, Ze-Uook
    • Applied Biological Chemistry
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    • v.29 no.2
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    • pp.122-129
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    • 1986
  • The lipid compositions of corns produced in Korea were analyzed. Free and bound lipids of the corn kernels were fractionated, quantitated and compared by silicic acid column, thin layer and gas liquid chromatography. Corn kernels contained 5.02% total lipids, which is consisted of 4.09% free lipids and 0.93% bound lipids. Free lipids comprised of 89.61% neutral lipids, 3.75% glycolipids and 6.40% phospholipids, while bound lipids contained 14.26% neutral lipids, 46.06% glycolipids and 37.18% phospholipids. In the neutral lipids of free lipids, triglycerides were predominant (67.68%) and minor components such as esterified sterols, free sterols, free fatty acids, 1,3-diglycerides, 1,2-diglycerides were present. But in the neutral lipids of bound lipids, esterified sterols were not present and the contents of triglycerides were lower (47.68%) and free fatty acids were higher than those of free lipids. Among the phospholipids in free and bound lipids, phosphatidyl choline and phosphatidyl serines and phosphatidyl inositols were also present as minor components. The major fatty acids in the three lipid classes were linoleic, oleic and palmitic acids.

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Design and Performance Analysis of a Parallel Optimal Branch-and-Bound Algorithm for MIN-based Multiprocessors (MIN-based 다중 처리 시스템을 위한 효율적인 병렬 Branch-and-Bound 알고리즘 설계 및 성능 분석)

  • Yang, Myung-Kook
    • Journal of IKEEE
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    • v.1 no.1 s.1
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    • pp.31-46
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    • 1997
  • In this paper, a parallel Optimal Best-First search Branch-and-Bound(B&B) algorithm(pobs) is designed and evaluated for MIN-based multiprocessor systems. The proposed algorithm decomposes a problem into G subproblems, where each subproblem is processed on a group of P processors. Each processor group uses tile sub-Global Best-First search technique to find a local solution. The local solutions are broadcasted through the network to compute the global solution. This broadcast provides not only the comparison of G local solutions but also the load balancing among the processor groups. A performance analysis is then conducted to estimate the speed-up of the proposed parallel B&B algorithm. The analytical model is developed based on the probabilistic properties of the B&B algorithm. It considers both the computation time and communication overheads to evaluate the realistic performance of the algorithm under the parallel processing environment. In order to validate the proposed evaluation model, the simulation of the parallel B&B algorithm on a MIN-based system is carried out at the same time. The results from both analysis and simulation match closely. It is also shown that the proposed Optimal Best-First search B&B algorithm performs better than other reported schemes with its various advantageous features such as: less subproblem evaluations, prefer load balancing, and limited scope of remote communication.

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Adaptive Consensus Bound PBFT Algorithm Design for Eliminating Interface Factors of Blockchain Consensus (블록체인 합의 방해요인 제거를 위한 Adaptive Consensus Bound PBFT 알고리즘 설계)

  • Kim, Hyoungdae;Yun, Jusik;Goh, Yunyeong;Chung, Jong-Moon
    • Journal of Internet Computing and Services
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    • v.21 no.1
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    • pp.17-31
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    • 2020
  • With the rapid development of block chain technology, attempts have been made to put the block chain technology into practical use in various fields such as finance and logistics, and also in the public sector where data integrity is very important. Defense Operations In addition, strengthening security and ensuring complete integrity of the command communication network is crucial for operational operation under the network-centered operational environment (NCOE). For this purpose, it is necessary to construct a command communication network applying the block chain network. However, the block chain technology up to now can not solve the security issues such as the 51% attack. In particular, the Practical Byzantine fault tolerance (PBFT) algorithm which is now widely used in blockchain, does not have a penalty factor for nodes that behave maliciously, and there is a problem of failure to make a consensus even if malicious nodes are more than 33% of all nodes. In this paper, we propose a Adaptive Consensus Bound PBFT (ACB-PBFT) algorithm that incorporates a penalty mechanism for anomalous behavior by combining the Trust model to improve the security of the PBFT, which is the main agreement algorithm of the blockchain.

Growth and Characterization of $ZnGa_2Se_4$ Single Crystal Thin Films by Hot Wall Epitaxy (Hot Wall Epitaxy(HWE)에 의한 $ZnGa_2Se_4$단결정 박막 성장과 특성에 관한 연구)

  • 장차익;홍광준;정준우;백형원;정경아;방진주;박창선
    • Korean Journal of Crystallography
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    • v.12 no.3
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    • pp.127-136
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    • 2001
  • A stoichiometric mixture of evaporating materials for ZnGa₂Se₄single crystal thin films was prepared from horizontal furnace. To obtain the single crystal thin films, ZnGa₂Se₄mixed crystal was deposited on thoroughly etched semi-insulating GaAs(100) substrate by the Hot Wall Epitaxy (HWE) system. The source and substrate temperatures were 610℃ and 450℃, respectively. The crystalline structure of the single crystal thin films was investigated by the photoluminescence and double crystal X-ray diffraction (DCXD). The carrier density and mobility of ZnGa₂Se₄single crystal thin films measured from Hall effect by von der Pauw method are 9.63×10/sup 17/㎤ and 296 ㎠/V·s at 293 K, respectively. From the photocurrent spectrum by illumination of perpendicular light on the c-axis of the ZnGa₂Se₄single crystal thin film, we have found that the values of spin orbit splitting △so and the crystal field splitting Δcr were 251.9meV and 183.2 meV at 10 K, respectively. From the photoluminescence measurement on th ZnGa₂Se₄single crystal thin film, we observed free excition (Ex) existing only high quality crystal and neutral bound exiciton (A°, X) having very strong peak intensity. Then, the full-width-at-half-maximum (FWHM) and binding energy of neutral acceptor bound excition were 11 meV and 24.4 meV, respectivity. By Haynes rule, an activation energy of impurity was 122 meV.

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Properties of Polymer-Modified Mortars Using Methylmethacrylate-Butyl Acrylate Latexes with Various Monomer Ratios (모노머비를 변화한 MMA/BA 합성 라텍스 혼입 폴리머 시멘트 모르타르의 성질)

  • Hyung, Won-Gil;Kim, Wan-Ki;Choi, Nak-Woon;Soh, Yang-Seob
    • Journal of the Korea Concrete Institute
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    • v.15 no.2
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    • pp.273-279
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    • 2003
  • The purpose of this study is to clarify the effect of the monomer ratio on properties of the polymer-modified mortars using methylmethacrylate-butyl acrylate(MMA/BA) latexes, and to obtain basic data necessary to develop appropriate latexes for cement modifiers. From the test results, we knew that the pore volume of polymer-modified mortars using MMA/BA latexes at bound MMA contents of 60 and 70 percent is 7.5∼75nm and the fine pore volume is increased with an increase in the polymer-cement ratio. The total pore volume of polymer-modified mortars using MMA/BA latexes is linearly reduced with an increase in the bound MMA content and increased in the polymer-cement ratio. In general, the superior compressive strength of polymer-modified mortars using MMA/BA latexes is obtained at a bound MMA content of 70 percent and a polymer-cement ratio of 15%. And, the water absorption and chloride ion penetration depth are greatly affected by the polymer-cement ratio rather than the bound MMA content. The important factors affecting the properties of polymer-modified mortars using MMA/BA latexes polymerized with various monomer ratios are the variations of the pore size distribution with changing bound MMA content and the polymer-cement ratio.

Fatty Acid Composition of Lipids obtained from Korean Soybean Varieties (한국산 콩의 품종별 지방질의 지방산 조성)

  • Yoon, Tai-Heon;Im, Kyung-Ja;Kim, Dong-Hoon
    • Korean Journal of Food Science and Technology
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    • v.16 no.4
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    • pp.375-382
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    • 1984
  • The fatty acid compositions of lipids from five local varieties and three imported varieties of soybean were determined. Total and free lipids of the samples were extracted with chloroform-methanol mixture (2:1, v/v) and ethyl ether, respectively. The ether-extracted samples were extracted again with water saturated n-butanol to obtain bound lipids. The average percent contents of the lipid fractions were 21% (total), 18% (free), and 3.3% (bound). The results indicated that seven fatty acids (10:0, 12:0, 14:0, 16:0, 17:0, 20:0 and 22:0) were identified as minor fatty acids for all the varieties examined in this study. The relative contents of 18:2 were the highest (50.1-59.7%) in all the lipid fractions, and the contents in total and free lipids were in-fluenced by the local varieties. Little difference in fatty acid composition was noted between the total and free lipids of the samples. Total and free lipids contained more 18:1 (about 10%) and less 16:0 (about 40%) and 14:0 (about 34%) than did the bound lipids. The relative percent contents of 18:3 in the total, free and bound lipids were on average 8.9, 9.0, and 7.3 percents. The content in the total lipids of Gwanggyo varieties showed the highest level (11.1%), and in the bound lipids of Eundaedoo varieties the lowest (6.0%). In case of the total and free lipids, those varieties which showed higher relative contents of 18:1 generally had lower relative contents of 18:2 and 18:3.

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Effects of Lower-Bound Resistances on Resistance Factors Calibration for Drilled Shafts (하한지지력이 현장타설말뚝의 저항계수에 미치는 영향)

  • Kim, Seok-Jung;Park, Jae-Hyun;Kim, Myoung-Mo
    • Journal of the Korean Geotechnical Society
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    • v.30 no.11
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    • pp.51-60
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    • 2014
  • Load and Resistance Factor Design (LRFD) is one of the limit state design methods, and has been used worldwide, especially in North America. Also, the study for LRFD has been actively conducted in Korea. However, the data for LRFD in Korea were not sufficient, so resistance factors suggested by AASTTO have been used for the design in Korea. But the resistance factors suggested by AASHTO represent the characteristics of bedrocks defined in the US, therefore, it is necessary to determine the resistance factors for designs in Korea, which can reflect the characteristics of bedrocks in Korea. Also, the calculated probabilities of failure from conventional reliability analyses which commonly use log-normal distribution are not realistic because of the lower tail that can be extended to zero. Therefore, it is necessary to calibrate the resistance factors considering the lower-bound resistance. Thus, this study calculates the resistance factors using thirteen sets of drilled shaft load test results, and then calibrates the resistance factors considering the lower-bound resistance corresponding to a target reliability index of 3.0. As a result, resistance factors from conventional reliability analyses were determined in the range of 0.13-0.32 for the shaft resistance, and 0.19-0.29 for the base resistance, respectively. Also, the lower bounds of resistance were determined based on the Hoek-Brown failure criteria (2002) and GSI downgrading. Considering the lower-bound resistances, resistance factors increased by 0~8% for the shaft, and 0~13% for the base, respectively.