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Intramuscular neural distribution of the teres minor muscle using Sihler's stain: application to botulinum neurotoxin injection

  • Kyu-Ho Yi;Soo-Bin Kim;Kangwoo Lee;Hyewon Hu;Ji-Hyun Lee;Hyung-Jin Lee
    • Anatomy and Cell Biology
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    • v.56 no.3
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    • pp.322-327
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    • 2023
  • The aim of this study was to elucidate the intramuscular arborization of the teres minor muslce for effective botulinum neurotoxin injection. Twelve specimens from 6 adult Korean cadavers (3 males and 3 females, age ranging from 66 to 78 years) were used in the study. The reference line between the 2/3 point of the axillary border of the scapula (0/5), where the muscle originates ant the insertion point of the greater tubercle of the humerus (5/5). The most intramuscular neural distribution was located on 1/5-3/5 of the muscle. The tendinous portion was observed in the 3/5-5/5. The result suggests the botulinum neurotoxin should be delivered in the 1/5-3/5 area of the teres minor muscle.

Study on the Midgut Epithelium of Korean Fresh Water Crayfish, Scanning Electron Microscopy (韓國의 민물가재(Cambaroides similis Koelbel)의 中腸上皮에 대한 走査電子顯微鏡的 考察)

  • Yeun, Kun-Seung;Noh, Yong-Tai
    • The Korean Journal of Zoology
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    • v.29 no.1
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    • pp.23-30
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    • 1986
  • Animals in Order Decapoda consist of a plentiful species and vary in external and internal shape, taste, feeding habit and the feature of alimentary canal. In this case, the morphological study on the midgut epithelium of filter feeding Decapoda in Crustacea, Korean fresh water crayfish (Cambaroides similis Koelbel), the only species dwelling in Korea, were performed by scanning electron microscopy. The anterior-most parts of midgut epithelium appeared to be many shallow, dense and irregular folds where a linear microvilli (MV) (1-3$\\mu$m long) numbered from a few to ten had raised. Middle part of the gut, the folds got gradually shallower. MV arranging on one, two or three lines were slightly thicker than those mentioned above. The number of MV in each group seemed gradual decrease from a apical border with maximum thirty in group to furrow with none. From the middle to posterior parts of the gut, the MV were slightly thinner and longer (2.5-4 $\\mu$m long) and raised twelve on a line and, in some cases, added from one to five on another line. On the posterior-end parts of the gut, the epithelium showed many shallow and dense folds as the anterior parts again. A group of thin and long MV (2-7 $\\mu$m long) countable fifteen on a line were raised to the base of each fold. In some cases, the MV also added utmost in five on the other line. All the MV observed in this study were posteriorly directed.

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Analysis of junction site between T-DNA and plant genome in Lissorhoptrus oryzophilus resistance GM rice (벼물바구미 (Lissorhoptrus oryzophilus) 내충성 GM 벼에서 T-DNA와 게놈의 인접부위 분석)

  • Lee, Jin-Hyoung;Shin, Kong-Sik;Suh, Seok-Cheol;Rhim, Seong-Lyul;Lim, Myung-Ho;Woo, Hee-Jong;Qin, Yang;Kweon, Soon-Jong;Park, Soon-Ki
    • Journal of Plant Biotechnology
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    • v.41 no.3
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    • pp.127-133
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    • 2014
  • Four transgenic rice lines harboring insect-resistant gene cry3A showed ideal field performances characterized by high considerable resistance to rice water weevil (Lissorhoptrus oryzophilus Kuschel). In this study, we estimated the insertion number of foreign genes, and analyzed the flanking sequences of T-DNAs in rice genome. As a result, T-DNA of BT12R1 line was inserted in exon region of rice chromosome 10. Two copies of T-DNAs were inserted in line BT12R2. BT12R3 line was analyzed at only left border flanking sequence. BT12R4 line was confirmed one copy of foreign gene insertion at the position 24,516,607 ~ 24,516,636 of rice chromosome 5, accompanied by a deletion of 30 bp known genomic sequences. This intergenic position was confirmed none of expressed gene and any deletion/addition of T-DNA sequence. In conclusion, these molecular data of rice water weevil resistant Bt rice would be used to conduct the biosafety and environment risk assessment for GM crop commercialization.

The vertical changes of the lip and perioral soft tissue resulting from incisor retraction (전치의 후방견인에 따른 입술과 주위 연조직의 수직적 변화)

  • Kang, Chang-Soo;Kim, Kyung-Ho;Choy, Kwang-Chul
    • The korean journal of orthodontics
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    • v.30 no.2 s.79
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    • pp.185-196
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    • 2000
  • Patients who want to reduce their lip protrusion usually estimate the severity of the lip protrusion on the frontal aspect. Most orthodontists have a perplexed experience of a reduced thin line of vermilion border on the frontal aspect as incisors we retracted, even though the lip protrusion is thought to be reduced favorably on the sagittal aspect. Some patients also look older after orthodontic treatment because of severe lip thinning. This unaesthetic reduction of vermilion border urges us to study the vertical lip change during orthodontic procedure. The purpose of this study was to evaluate the vertical lip and perioral soft tissue changes in respect to incisor retraction in an effort to analyze which factors might be responsible for their vertical changes, using the multiple regression analysis. The results were as follows. 1. Upper and lower lip philtrum length(SnLs, LiB') were increased after retraction of anterior teeth, where as upper and lower vermilion height(LsSuls, StmiLi), and vermilion length(LsLi) were decreased. 2. Upper and lower lip length(SnStms, StmiB'), and soft tissue lower anterior facial height(SnMe') did not show any significant difference after treatment. 3. The increase of the upper lip philtrum length was mainly influenced by the extrusion of upper anterior teeth(${\Delta}U1V$), and the increase of the lower lip philtrum length was mainly influenced by the initial overjet before treatment. 4. The decrease of the upper and lower lip vermilion height was mainly influenced by the decrease of upper lip thickness.

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Mechanism of Inhibition of ${\alpha}$-Methylglucose Uptake by Cisplatin in $LLC-PK_1$ (시스플라틴에 의한 $LLC-PK_1$의 알파-메틸글루코스 흡수 감소 기전)

  • Seo, Kyung-Won;Kim, Hyo-Jung;Choung, Se-Young
    • YAKHAK HOEJI
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    • v.40 no.6
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    • pp.705-712
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    • 1996
  • We have previously shown that determination of glucose uptake using ${\alpha}$-methylglucose(${\alpha}$-MG) is very sensitive and rapid parameter for the assessment of loss of cellular fu nction in renal cell line($LLC-PK_1$). The present study was designed to elucidate the mechanism of inhibition of ${\alpha}$-MG uptake and the intracellular site of toxic action of cisplatin(CIS). $LLC-PK_1$ cells were exposed to various concentrations(5 ${\mu}$M-l00 ${\mu}$M) of CIS for 5 hrs or 24 hrs and ${\alpha}$-MG uptake was determined. Mitochondrial function was evaluated by measuring intracellular ATP content and MTT reduction. The activities of marker enzymes for the basolateral membrane(Na$^+$-K$^+$ ATPase) and brush border membrane (alkaline phosphatase: ALP) were also measured. CIS treatment significantly inhibited the ${\alpha}$-MG uptake in a time- and dose-dependent manner above 25 ${\mu}$M for 5 hrs. Intracellular ATP content and MTT reduction were affected by 24 hr-treatment of 50 ${\mu}$M CIS. The activities of Na$^+$-K$^+$ ATPase and ALP were significantly decreased at 10 ${\mu}$M and 5 ${\mu}$M of CIS for 24 hrs, respectively. The incubation with CIS for 5 hrs had no effects on the intracellular ATP content, MTT reduction and the activities of marker enzymes up to 100 ${\mu}$M. These results partly indicate that inhibition of ${\alpha}$-MG uptake by CIS may not be attributed to the disturbance of mitochondrial function or inhibition of the activity of Na$^+$-K$^+$ ATPase and can be resulted from direct effect of CIS on the Na$^+$/glucose cotransporter in brush border membrane. This study shows that additional mechanistic information, indicating the intracellular site of nephrotoxic action, can be gained by coupling the ${\alpha}$-MG uptake and ATP content or the activity of Na$^+$-K$^+$ ATPase.

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A STUDY OF MANDIBULAR ANATOMY FOR ORTHOGNATHIC SURGERY IN KOREANS (악교정 수술을 위한 한국인 하악지의 해부학적 위치에 관한 연구)

  • Woo, Soon-Seop;Cho, Jung-Yeon;Park, Won-Hee;Yoo, Im-Hag;Lee, Young-Soo;Shim, Kwang-Sup
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • v.28 no.2
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    • pp.126-131
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    • 2002
  • Anatomical shape of the mandibular ramus, which includes the area from the rear of the mandibular second molar to the mandibular posterior border and from the mandibular sigmoid notch to the inferior mandibular border, must be carefully considered to perform orthognathic surgery. The locations of the lingula and mandibular foramen in medial side of mandibular ramus are one of the most important factors to decide the location of the horizontal medial osteotomy in sagittal split ramus osteotomy and to select the line of vertical osteotomy in intraoral vertical ramus osteotomy. Sixty-five different Korean human dry mandibles were surveyed. All mandible have permanent dentition including complete eruption of the mandibular second molar. The locations of the lingula and mandibular foramen in medial side of the ramus were identified and following results were obtained. Anterior ramal horizontal distance from lingula was $16.13{\pm}3.53mm(range:8.6{\sim}24.3mm)$, anterior ramal horizontal distance from mandibular foramen was $23.91{\pm}4.79mm(range: 14.1{\sim}39.7mm)$, horizontal width of mandibular foramen was $2.79{\pm}0.95mm(range:1.5{\sim}6.1mm)$, height of lingula was $10.51{\pm}3.84mm(range:3.1{\sim}22.4mm)$, vertical distance from sigmoid notch to lingula was $19.82{\pm}5.11mm(range:9.1{\sim}35.3mm)$. From this study, the result could be used to select the location of osteotomy lines and to decide amount of periosteal elevation to avoid injury of neurovascular bundle, and to accomplish the appropriate split in Korean patients in mandibular orthognathic surgery.

Correction of Retracted Ala Using Spacer Graft in Secondary Cleft Lip and Nose Deformity (이차 구순비 변형 환자에서 Spacer Graft를 이용한 콧방울뒤당김(retracted ala)의 교정)

  • Han, Kyu-Seok;Choi, Hyun-Gon;Shin, Dong-Hyeok;Kim, Soon-Heum;Hwang, Eun-A;Uhm, Ki-Il
    • Archives of Plastic Surgery
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    • v.38 no.4
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    • pp.376-382
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    • 2011
  • Purpose: In patients with unilateral cleft lip and nose deformity, alar retraction is commonly seen on the non-cleft side after cleft side is corrected. Spacer graft was used to drag down the inferior border of the alar cartilage of the non-cleft side so as to match the cleft side. By performing spacer graft and septal extension graft together, symmetry and cosmetic improvements were achieved. Methods: Seven unilateral cleft lip and nose deformity patients underwent surgery for alar retraction correction. The median age was 24 years (ranged from 15 to 34 years), and the median follow-up period was 7.4 months (ranged from 6 to 12 months). The perpendicular length from the longitudinal axis of the nostril to the alar rim, the nasolabial angle and the ala-labial angle were measured in the lateral view photo. The longest perpendicular length from the cephalic border of the alar rim to the parallel line of the alar base was measured in the frontal view photo. Results: Improvement in alar retraction was seen after the surgery. There were no specific complications during the follow-up and the symmetry of both nostrils was satisfactory. No increase in the nasolabial angle or exposure of the nostrils was seen after the tip projection via tip plasty. Conclusion: The fundamental factor in correcting alar retraction with secondary cleft lip and nose deformity is repositioning the alar rim with spacer graft, which seems to be more physiologic than other methods. The method combining spacer graft with septal extension graft will bring symmetry as well as more cosmetic improvement in correction of alar retraction with secondary cleft lip and nose deformity.

The Development of Contemporary Fashion Designs Specialized in the Traditional Costume of the Miao (중국 묘족(苗族) 복식을 특화시킨 현대적 패션 디자인 개발)

  • Lee, Keum-Hee;Yoon, Ji-Won;Han, Jung-Ah
    • The Research Journal of the Costume Culture
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    • v.15 no.5
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    • pp.902-915
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    • 2007
  • This study examines decorative features found in the traditional costume of the Miao, one of the minority groups in China, and adapts them for the development of modern Korean-style fashion designs in order to present visual sample data and to introduce techniques. As for the research method, both literature and visual data are examined: the former includes Miao-related books and theses; the latter, pictures from various documents and real-life data in the possession of Seoul Women's University. The study result is as follows: 1. The basic structure of Miao costume is consist of a Caftan-type upper garment and an accentuated lower garment, pleats skirt although they are decorated luxuriously with various techniques such as delicate embroidery and dyeing. 2. Details and trimmings in modern shapes are developed and presented by adding modern materials to satin, twists, cross-stitch embroidery, stripe patterns, geometrical patchwork, machine pleats and smoking, trimmings of fringe & feather, and the border line decoration of coloration, braids, piping, embroidery, belts tied and wound with colorful threads. 3. Detachable accessaries such as front reinforcement strips, back reinforcement strips, belts, decorative aprons and patchwork skirts are developed and designed by adapting highlighted layered effects. 4. The silver decoration used as an necessary by the Miao is suitable for modern fashion design in futuristic sense. Various techniques and decorative features of Miao costume display their true value all the more in modern fashion stream in which ethnic trends draw much attention.

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A Study on the Design Image of Men's Shirts Expressed in High Fashion (하이패션에 나타난 남성셔츠 디자인 이미지 연구)

  • Kim, Hyun-A;Lee, Hyo-Jin
    • Journal of the Korean Society of Costume
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    • v.61 no.1
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    • pp.20-33
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    • 2011
  • This study aims to analyze the expressive aspect of men's shirts through examining the characteristics of men's shirts with the trend of men's clothing well represented and considering the image based on high fashion. As a method of study, I have conducted the theoretical study through the literature data, and the content analysis through photographs from S/S collection in 2005 to F/W collection in 2010. Especially, in the recent men's shirts, the materials of glossy silk, wavering chiffon and jersey tend to appear and various images are represented with the tasteful decoration such as ruffle, pin tuck and embroidery together with pastel color. Thus, the result of this study is as follows: Firstly, the masculine and romantic images are researched as the image emphasizing the beautiful and soft masculine beauty which doesn't lose the masculinity even though they are seasonedwith the feminine elements. Soft masculine beauty is emphasized by using romantic image decoration of frills, beads and pin tuck or silk, jersey and openwork materials etc. Secondly, practical fusion images have been studied as the images emphasizing the freshness with practicality through grafting more than two items onto one shirt. Many designs have appeared representing new shapes by grafting more than two items onto one shirt or obscuring the border line between the items. Thirdly, the avant-garde chic images have been researched as the image emphasizing the chick image through representing unique and odd designs by excessively expressing the silhouette in general shapes of shirts. I come. to find that the mainly the designs, emphasizing the uniqueness and the distinction with transformed shapes of sleeves, cuffs and the length of shirts in exaggerated manners, are appearing. Thus, I intend to provide a reference material which will be useful for the newly created design and researches of figurative characteristics in the future fashion.

Reconstruction of Trochanteric Pressure Sores using Perforator-based Flap from the Ascending Branch of Lateral Circumflex Femoral Artery (외측대퇴회선동맥 상행가지의 천공지피판을 이용한 대전자부 욕창의 재건)

  • Kim, Jun-Hyung;Eo, Su-Rak;Cho, Sang-Hun
    • Archives of Plastic Surgery
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    • v.37 no.5
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    • pp.595-599
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    • 2010
  • Purpose: Trochanteric pressure sores management has been improved through the development of musculocutaneous flaps. But it has many drawbacks such as donor site morbidity and functional muscle sacrifice. With the introduction of perforator flap, it is possible to use in every location where musculocutaneous perforators are present. We have reconstructed trochanteric pressure sores using perforator-based flaps from the ascending branch of lateral circumflex femoral artery. Methods: Between May of 2006 and April of 2008, we performed six cases of perforator-based flap from the ascending branch of lateral circumflex femoral artery for the coverage of trochanteric pressure sores. For identifying perforators, a line was drawn from the anterior superior iliac spine to the superolateral border of the patella as the vertical axis, from the pubis to the trochanteric prominence as the horizontal axis. In the lateral aspect of the intersection of these two axes, various flap were designed according to its defects. The flap was raised in the subcutaneous plane above the fascia and the pedicle was traced by doppler and identified. The pedicle was meticulously dissected not to injure the periadventitial tissues and transposed to the defect. The donor site was closed primarily. Results: The mean age of patients was 56.2 years. Four male and two female patients were studied. Five patients were paraplegic. The mean defect size was $6{\times}4\;cm$. The largest flap dimension was $14{\times}7\;cm$. Donor sites were closed primarily without any complications. All flaps survived completely without necrosis, hematoma or infection. There were no recurrence during the follow-up period. Conclusion: Trochanteric pressure sores using perforator-based flap from the ascending branch of lateral circumflex femoral artery can be performed safely and it would be a reliable option for coverage of trochanteric pressure sores with minimal donor site morbidity.