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Physio-chemical impacts on efficient germination in Platycodon grandiflorum for. duplex pollen culture

  • Kwon, Soo Jeong;Hwang, Ha Nule;Moon, Young Ja;Cho, Gab Yeon;Woo, Sun Hee;Boo, Hee Ock;Koo, Jin-Woog;Kim, Hag Hyun
    • 한국작물학회:학술대회논문집
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    • 한국작물학회 2017년도 9th Asian Crop Science Association conference
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    • pp.170-170
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    • 2017
  • The highly valued ornamental plant, Platycodon grandiflorum for. duplex was generated by petaloidy of a calyx of Platycodon grandiflorum. The present investigation was executed to explore the several factors having effects on the germination of pollens with a view to acquire the underlying data for the artificial crossing to cultivate the species of Platycodon grandiflorum for. duplex. Both low and high temperature impaired the germination of Platycodon grandiflorum for. duplex pollens. The good germination rate was observed at the temperature of $25^{\circ}C$. The types and concentrations of carbon sources induced the differences in germination rate. The germination rate increased with the increasing concentration of sucrose and glucose except for fructose. Sucrose and glucose showed the highest results at the concentration of 20%. While fructose demonstrated the similar tendency to sucrose and glucose, it reduced the germination rate at the concentration of 20%. The highest germination rate was observed at the concentration of 15%. The appropriate carbon course for germination of pollens of Platycodon grandiflorum for. duplex was glucose of which germination rate was twice as high as that of sucrose and fructose. The germination rate was reduced substantially when the pH was close to alkali, and the potential germination rate was obtained at pH 6. Boric acid enhanced the germination rate at a lower concentration than the higher concentration.

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구연산, 테트라싸이클린, 섬유소 전색제로 처리된 상아질면에 대한 결체조직의 조기부착 (EARLY CONNECTIVE TISSUE ATTACHMENT ON DENTIN SURFACE TREATED WITH CITRIC ACID, TETRACYCLINE AND FIBRIN SEALANTS)

  • 이혜자;한수부;고재승
    • Journal of Periodontal and Implant Science
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    • 제23권2호
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    • pp.283-299
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    • 1993
  • The purpose of this study was to observe early connective tissue attachment on dentin surface treated with citric acid, tetracycline, and fibrin sealants and compare their conditioning effects on dentin surface. Experimental dentin blocks conditioned with citric acid, tetracycline or fibrin sealant, and only root planned control block were surgically implanted in the pouch under buccal mucoperiosteal flaps of left mandible, right maxilla, left maxilla, right mandible of 18 male rabbits. Rabbits were sacrificed after 1 and 6 hours, 1, 3, 7 and 14 days after implantation and then specimens including dentin block and surrounding soft tissue were obtained, and prepared for light and transmission electron microscopic examination. 1 and 6 hours after dentin block implantation, there was plasma proteins adsorption followed by fibrin clot formation and no differences among specimens. At the 1-day observation interval, delicate fibrin network was observed in the all groups, and there were proliferative fibroblasts, angiogenesis and macrophage in the all 3-day specimens. Cellular aggregates and abundant connective tissue adhered dentin surface and tetracycline or citric acid treated group showed much proliferative fibroblast and abundant collagen fibers at 1 week. But at 2 week, citric acid treated group showed much proliferative fibroblast and abundant collagen fibers. These observations suggested that new connective tissue attachment to dentin was initiated by the adsorption of plasma proteins to the dentin surface and followed by fibrin clot formation. Tetracycline and citric acid seemed to make dentin surface more biologically favorable for the connective tissue attachment.

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양식 붉바리(Epinephelus akaara) 종자의 개발과 사양관리 (Management and Development of Seed Rearing in Red Spotted Grouper Epinephelus akaara)

  • 부문수;이치훈;문영건;김병훈;김민석;김대근;백혜자;김형배;이영돈
    • 한국수산과학회지
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    • 제53권4호
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    • pp.506-514
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    • 2020
  • We investigated seed management and feeding regime of larval red spotted grouper Epinephelus akaara using live food organisms. Fertilized eggs were produced in March 2020 by manipulating photoperiod and water temperature. Broodstock (F0) produced approximately 0.30×106 eggs, of which 0.25×106 were fertilized; first-generation broodstock (F1) produced 2.00×106 eggs, of which 1.90×106 were fertilized. Larvae were reared at temperatures of 21.0-24.5℃. The feeding regime consisted of rotifers Artemia, and commercial pellet feed. From 5-8 days after hatching (dah), rotifers collected from high-density incubation tanks were fed to grouper larvae at a density of 10-20 individuals/mL. From 9-30 dah, rotifers collected from low-density incubation tanks were fed to larvae at a density of 10-20 individuals/mL. Newly hatched Artemia were provided from 20-24 dah and enriched Artemia from 25-50 dah. Commercial pellet feed was given starting at 13 dah. The average mouth size in hatchlings was 263.4±3.7 ㎛ at 5 dah, 406.7±21.0 ㎛ at 11 dah, and 856.9±136.6 ㎛ at 21 dah. At 1 dah, larvae had a mean total length of 1.95±0.06 mm. At 66 dah, the mean TL was 4.0 cm. The final number of surviving red spotted grouper larvae was 218,380 fishes.

Isolation and Characterization of Strain of Bacillus thuringiensis subsp. kenyae Containing Two Novel cry1-Type Toxin Genes

  • Choi, Jae-Young;Li, Ming Shun;Shim, Hee-Jin;Roh, Jong-Yul;Woo, Soo-Song;Jin, Byung-Rae;Boo, Kyung-Saeng;Je, Yeon-Ho
    • Journal of Microbiology and Biotechnology
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    • 제17권9호
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    • pp.1498-1503
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    • 2007
  • To identify novel crystal proteins, Bacillus thuringiensis 2385-1 was isolated from Korean soil samples and characterized. The H-serotype of 2385-1 was identical to that of subsp. kenyae (H4a4c), and its crystal toxin was bipyramidal-shaped. However, 2385-1 showed a much higher toxicity towards Plutella xylostella and Spodoptera exigua larvae than subsp. kenyae. In addition, the crystal protein profile and plasmid DNA pattern of 2385-1 differed from those of subsp. kenyae. To verify the crystal protein gene types of 2385-1, a PCR-RFLP analysis was performed, and the results revealed that 2385-1 contained two novel cry1-type crystal protein genes, cryl-5 and cry1-12, in addition to the crylJal gene. The deduced amino acid sequences of cryl-5 and cry1-12 showed a 97.9% and 75.7% sequence similarity with the CrylAb and CrylJa crystal proteins, respectively. Among the novel crystal proteins, Cry1-5 showed a high toxicity towards P. xylostella and S. exigua larvae. In conclusion, B. thuringiensis 2385-1 is a new isolate in terms of its gene types, and should be a promising source for an insecticide to control lepidopteran larvae.

모 선박용 디젤엔진 제조업체 들기작업의 인간공학 위험요인 평가 (Assessment of Ergonomic Risk Factors of Manual Material Handling in the Ship Diesel Engine Assembling Processes)

  • 김부욱;김선자;신용철;김현동;우지훈;강동묵;이현석
    • 한국산업보건학회지
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    • 제15권3호
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    • pp.153-159
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    • 2005
  • The purpose of this study was to assess the ergonomic risks of lifting tasks in a marine diesel engine manufacturing industry using the National Institute for Occupational Safety and Health(NIOSH) Revised Lifting Equation(NLE). Average Lifting Index(LI=Weight of Load/Recommended Weight Limit) of a total number of 45 lifting tasks was $1.6{\pm}0.7$. The LIs were above 1 at 34 tasks(75.6%), and above 2 at 11 tasks(24.4%). Parts management showed the highest average LI value (LI=2.3) in all departments, which resulted from high frequency and heave load of lifting. The common and significant ergonomic risk factors in the processes were the heavy weight of diesel engine parts and the long horizontal distance. In addition, some lifting tasks had such potential risk factors as the long vertical distance, the high frequency of lifts or the long work duration.

절패모(浙貝母) 에탄올 추출물의 멜라닌 생성 억제 효과 (Inhibitory Effect of Fritillaria Verticillata Willd. var. Thunbergii Bak Ethanol Extract on Melanin Biosynthesis)

  • 하태광;이부균;윤정록;문연자;우원홍;박성하;이장천
    • 동의생리병리학회지
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    • 제25권3호
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    • pp.510-515
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    • 2011
  • This study was conducted to evaluate the depigmenting properties of ethanol extract from a Fritillaria verticillata Willd. (EFV) in B16F10 cells. Fritillaria verticillata Willd., a perennial herbaceous plant, has been used as a stimulator of mammary gland, expectorant, blood pressure depressant, antitussive agents in Korean herbal medicine. In the present study, we observed that melanin synthesis of B16F10 cells were significantly decreased by EFV without cytotoxicity. However, EFV could not suppress tyrosinase activity in B16F10 cells and mushroom tyrosinase activity. Furthermore, EFV did not effect the protein expression of tyrosinase, tyrosinase-related protein -1 (TRP-1), and TRP-2. These results suggest that EFV inhibited melanin synthesis and the hypopigmentary effect of EVF was not due to regulation of tyrosinase protein.

왼손 및 오른손 사용자(使用者)의 상반신체형(上半身體型)에 관한 비교연구(比較硏究) - 20대(代) 남녀(男女) 대학생(大學生)을 중심(中心)으로 - (Upper Body Somatotypes of the Left-Handed and the Right-Handed - Focusing on male and female collegians in their 20s -)

  • 심부자
    • 패션비즈니스
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    • 제7권4호
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    • pp.78-92
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    • 2003
  • The upper-body characteristics of the right-handed and the left-handed are examined and compared through 1-dimensional measurement and 2-dimensional measurement. The results were as follows: 1. According to 1-dimensional measurement results, the clearest differences were seen in girth items rather than height or length items. In particular, hand girth, lower arm girth, and upper arm girth were bigger on the left side in the left-handed group and the right side in the right-handed group. This is thought to be relevant to the arm muscles frequently used. 2. According to 2-dimensional measurement results, significant differences appeared in the females of the left-handed group: shoulder point among width items. In the right-handed group, some differences of significance were shown in females (shoulder point, rear armpit point, and B.P. point among width items) and males (front neck point among extreme vertical distance items). In summary, the body type characteristics of the left-handed and the right-handed reveal great differences in the items related to arm length and arm girth. In other words, the sleeve and shoulder patterns production based on the right-handed don't rightly reflect the somatotype characteristics of the left-handed. In addition, in the cases of athletes, laborers, and the disabled who frequently use particular muscles or parts of the body, this sort of research in the patterns is consistently needed in order to reflect the size differences in girth items in upper body.

교육용(敎育用) 성인(成人) 남성(男性)의 Dress Form 개발(開發)을 위한 기초연구(基礎硏究) - 남성복업체(男性服業體)의 Dress Form 사용현황(使用現況) 및 남성복(男性服) 교육실태(敎育實態) 분석(分析)을 통(通)해 - (A Basic Study for the Development of Educational Dress Forms for Male Adults - Survey of Male Dress Forms for Men's Apparel Company and the Education for Men's Wear in Fashion Dept. of University -)

  • 유현;심부자
    • 패션비즈니스
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    • 제8권5호
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    • pp.100-114
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    • 2004
  • This is a basic study for developing educational dress forms of male adults' pattern making. While examining the reality of each university's men's wear education and the production of male dress forms, this paper found the following findings: 1. Survey of Dress Forms Used by Men's Apparel Companies The men's apparel companies had dissatisfaction with domestic dress forms but satisfaction with foreign-made ones. In general, among the unsatisfied items were wide discrepancy in male Korean body sizes, unfitting domestic somatotypes, lacking dress forms for those in the 20s, and no diverse forms per body type. To be specific, unsuitable hip and shoulder parts as well as weak fixing parts were pointed out. Every apparel companies found it necessary to develop male dress forms based on Korean male adults. When they are developed for sale, they will be highly utilized in the order of fitting, pattern making, male design research, and somatotype research. 2. Survey of Education for Men's Wear in Fashion Dept. of University 61.54% of the investigated universities had some teaching as far as men's wear were concerned. Application areas of male dress forms were such as pattern making (53.85%), fitting (41.38%), and design research (5.77%). As to the degrees of utilization, 73.08% predicted them high.

The Wearing Sense of Male Adult Shoes - Comparison of Common Shoes with Elevated Shoes -

  • Shim, Boo-Ja;Yoo, Hyun
    • 패션비즈니스
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    • 제11권6호
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    • pp.35-51
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    • 2007
  • This research was administered in order to know the effects of heels on the foot by comparing the foot environmental characteristics when common shoes and elevated shoes are worn. First, 157 male adults in their 20s through 40s living in Busan were the inquiry subjects to reveal the shoes-wearing reality of adult males. Second, 7 male adults in their early 20s became the subjects for the experiments of wearing common shoes and elevated shoes. 1. Inquiry Results of Shoes-Wearing Reality Common-shoes wearers were in the order: 20s (43.9%) > 30s (24.8%) > 40s (8.3%). Elevated-shoes wearers were mostly 20s (12.1%), followed by 30s (8.3%) and 40s (2.5%). Among the wearing effects of elevated shoes were 'looking taller' (66.7%), 'no height complex & more confidence' (30.6%), and 'higher work efficiency' (2.8%). In sum, 97.3% of the male subjects believed in great positive effects by wearing elevated shoes. 2. Shoes-Wearing Experiment Results In foot skin temperature, significant differences between the two groups were admitted in outer foot a (p<0.05) and other areas (p<0.001), except in the instep. Elevated-shoes group had bigger skin temperature, while the order of temperature was the instep, the big toe, inner foot a/b/c and outer foot a/b/c. Significant difference was accepted in total sweat rate (p<0.05) and local sweat rate (p<0.01). Elevated-shoes group appeared higher in both rates. Significant difference (p<0.001) between the two groups was recognized in fatigue degrees after wearing, whereas significance (p<0.05) in elevated-shoes group was approved in fatigue before and after exercise. So elevated-shoes group experienced more fatigue, especially after exercise.

패턴분석(分析) 및 착의평가(着衣評價)에 따른 엔지니어드 진 팬츠의 기능성(機能性) 연구(硏究) (A Study on the Functionality of Engineered Jean Pants According to Pattern Analysis and Clothing Evaluation)

  • 심부자;서추연;유현
    • 패션비즈니스
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    • 제9권4호
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    • pp.145-160
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    • 2005
  • On the subjects of the female adults in their 20s who love to wear jeans, the wearing reality of basic jean pants and engineered jean pants was examined while pattern comparison and the evaluation of appearance and functions were made to reach the following conclusions: 1. Wearing reality of engineered jean pants Among the examined subjects, 74.8% were found out to favor engineered jean pants. Those who have engineered jean pants turned out to pursue well-known brands more than practicality. 2. Pattern comparison analysis of basic jean pants and engineered jean pants Though similar sizes existed in pants tips and belt width, engineered jean pants showed bigger values in waist circumference, crotch circumference, hip circumference, knee circumference, thigh circumference and so on. There were no big differences in the front part except for the items like crotch circumference and crotch length, but engineered jean pants had bigger sizes in the rear part. Engineered jeans in the rear contained the outward curved silhouette of the legs, curved tips, and a dart design instead of a back yoke. 3. Evaluation of appearance and functions In the scores of appearance, basic jean pants were higher at 3.65 in the order of front > side > back. On the contrary, engineered jean pants were excellent in the order of side > back > front. In the items of functions, engineered jean pants were higher at 4.23 in the order of hips > thighs> waist > abdomen > knee > crotch. On the other hand, basic jean pants showed the order of knee > crotch & thighs > abdomen > hips > waist. In every movement, engineered jean pants revealed higher functionality. In particular, the bigger physical movement led to the greater functional differences between the two kinds of jean pants.