• Title/Summary/Keyword: bone growth

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Expression and Purification of Recombinant Human Bone Morphogenetic Protein-7 (rhBMP-7) in Bacillus subtilis (고초균을 이용한 재조합 인간 골 형성 단백질-7의 발현과 정제)

  • Kim, Chun-Kwang;Oh, Sung-Duk;Rhee, Jong-Il
    • KSBB Journal
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    • v.25 no.3
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    • pp.257-264
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    • 2010
  • Bone morphogenetic protein-7 (BMP-7) is one of important growth factors for skeletal development and bone growth. In this work, BMP-7 was efficiently expressed in recombinant Bacillus subtilis. The mature BMP-7 protein indicated molecular weight of 15.4 kDa by Western blot assay and was secreted into culture medium with 0.35 ng/mL. The extracellular and intracellular rhBMP-7 proteins were purified by using a FPLC system with an ion exchange column and a gel filtration column. The extracellular and intracellular rhBMP-7 proteins had finally a 57.1% purity and a 36.2% purity, respectively. The purified rhBMP-7 proteins showed an intact biological activity which stimulated alkaline phophatase (ALP) activity in MC3T3-E1 cells.

A LONGITUDINAL STUDY OF CHANGE OF THE GONIAL ANGLE ACCORDING TO MAXILLARY AND MANDIBULAR BONE GROWTH IN KOREAN CHILDREN (두부방사선 계측사진을 이용한 한국아동의 상,하악골 성장에 따른 Gonial angle의 변화에 관한 연구)

  • Ryu, Young-Kyu
    • The Journal of the Korean dental association
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    • v.19 no.7
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    • pp.615-623
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    • 1981
  • The purpose of this study was observing the annual growth increment in Cephalogram. The Cephalograms of 28 boys & 22 girls were analized by method of Downs & Steiner, range from 7 to 9 years in Korean children. The results were as follows. 1. The change of gonial angle in both male and female is not presented in this age. 2. The most significant linear change of both male and female is the facial depth (Na-Go) and that of both male and female is the interincisal angle in this age. 3. The increment of the mandibular body length (Go-Me) and the anterior facial height (Na-Me) is presented in this age. 4. The change of SNA and SNB in both male and female is negligible is negligible in this age.

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Autotransplantation in patient who is not indicated fixed prosthesis: case report (고정성 보철 수복이 부적절한 환자에서 자가치아이식을 응용한 치험례)

  • Baek, Sang-Hyeon;Jeong, Hye-Sim;Seong, Sang-Jin;Mun, Yun-Sik;Jang, Hyeon-Ho;Ra, Yun-Sik
    • The Journal of the Korean dental association
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    • v.42 no.8
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    • pp.514-522
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    • 2004
  • Clinicians often meet the growing patient who is not treated with fixed prosthesis until alveolar bone growth is completed. When both esthetic and function needs are important, and if proper donor tooth is present, the application of autotransplantation may be better treatment option than space maintainer. Although prognosis of autotransplantation is not always favorable, it depends on the treatment design which is based on the ability of case selection and consideration. This report will present the clinical cases of autotransplantation in growing patient, and discuss about several clinical aspects of autotransplantation.

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Condylar hyperplasia: An updated review of the literature

  • Almeida, Luis Eduardo;Zacharias, Joseph;Pierce, Sean
    • The korean journal of orthodontics
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    • v.45 no.6
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    • pp.333-340
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    • 2015
  • Condylar hyperplasia (CH) is a rare disorder characterized by excessive bone growth that almost always presents unilaterally, resulting in facial asymmetry. Classification of the different types of CH can differ depending on the authors. Correct diagnosis is critical in determining the proper treatments and timing. This paper is a review of the recent literature on the epidemiology, etiology, diagnosis, classification, and surgical treatments of CH.

Poly(l-lactide) membranes with biomimetic nanolayer for bone induction for tissue regeneration

  • Chung , Ji-Eun;Lee, Jue-Yeon;Kim, Kyung-Hwa;Baek, Hyun-Jin;Ku , Young;Chung, Chong-Pyung;Lee, Seung-Jin
    • Proceedings of the PSK Conference
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    • pp.226.2-226
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    • 2003
  • .The healing of a bone defect is complex, and involves a wide range of cellular, molecular, physiological, and biological processes. The main effect of bone substitute is to promote wound healing by induce cell proliferation. Bone defect sites usually are localized below the original bone surface; therefore, space production and maintenance between the membrane and the original bone surface is essential. As a result, membranes must have proper mechanical strength to prevent the collapse of the soft tissue and maintain wound space that permits membranes of poly (L-lactide) (PLLA) were fabricated to provide and maintain sufficient space for bone growth. (omitted)

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A Case Report of Guided Bone Regeneration Using a Putty-type Demineralized Bone Matrix (골유도재생술에 대한 putty형 탈회 기질골 이용연구)

  • Jeong, Mi-Ae
    • Proceedings of the KAIS Fall Conference
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    • pp.330-333
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    • 2011
  • Allomatrix (Wright Medical Tech, Inc., USA), is a newly designed, injectable putty with a reliable demineralized bone matrix(DBM), derived from human bone. The compound contains 86% DBM and other bone growth factors such as bone morphogenic protein (BMP)-2, BMP-4, insulin-like growth factor (IGF)-1, and transforming growth factor (TGF)-${\beta}1$. It has excellent os-teoinduction abilities. In addition, DBM is known to have osteoconduction capacity as a scaffold due to its collagen matrix. This product contains a powder, which is a mix of DBM and surgical grade calcium sulfate as a carrier. A practitioner can blend the powder with calcium sulfate solution, making a putty-type material which has the advantages of ease of handling, better fixation, and no need for a membrane, because it can function as membrane itself. This study reports the clinical and radiographic results of various guided bone regeneration cases using Allomatrix, demonstrating its strong potential as a graft material.

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Effects of Sustained-Release Formulation of Recombinant Human Growth Hormone on Body weight. Bone growth and Organs in Hypophysectomized Rats

  • Jo, Yeong-Woo;Park, Yong-Man;Lee, Ghun-Il;Park, Yong-Man;Yang, Hi-Chang;Kim, Mi-Ryang;Lee, Sung-Hee;Kwon, Jong-Won;Kim, Won-Bae
    • Proceedings of the PSK Conference
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    • pp.417.2-418
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    • 2002
  • The rhGH-loaded PLGA microsphere formulation was prepared using a double emulsion process from hydrophilic 0:50 poly(D.L-lactide-co-glycolide) (PLGA) polymers. To investigate the sustained efficacy of this formulation, ts pharmacodynamic characteristics were analyzed. It showed particle size of ca 53.1 $\mu\textrm{m}$ with high drug ncorporation efficiency and it was sucutaneously administrated to hypophysectomized rats and whole body rowth responses of this formulation were compared to those of the different dosing patterns of rhGH. (omitted)

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Microstructure Control of HAp Based Artificial Bone Using Multi-extrusion Process

  • Jang, Dong-Woo;Lee, Byong-Taek
    • Proceedings of the Materials Research Society of Korea Conference
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    • pp.54.1-54.1
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    • 2011
  • Porous hydroxyapatite has been widely used as clinical implanted material. However, it has poor mechanical properties. To increase the strength as well as the biocompatibility of the porous HAp based artificial bone, it was fabricated by multi-extrusion process. Hydroxyapatite and graphite powders were mixed separately with ethylene vinely acetate and steric acid by shear mixing process. Hydroxyapatite composites containing porous microstructure were fabricated by arranging it in the die and subject it to extrusion process. Burn-out and sintering processes were performed to remove the binder and graphite as well as increase the density. The external and internal diameter of cylindrical hollow core were approximately 10.4 mm and 4.2 mm, respectively. The size of pore channel designed to increase bone growth (osteconduction) was around 150 ${\mu}m$ in diameter. X-ray diffraction analysis and SEM observation were performed to identity the crystal structure and the detailed microstructure, respectively.

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Advanced Surface Modification Techniques for Enhancing Osseointegration of Titanium Implant (임상가를 위한 특집 1 - 티타늄 임플란트의 골융합 증진을 위한 최신 표면처리 기술)

  • Song, Ho-Jun
    • The Journal of the Korean dental association
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    • v.48 no.2
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    • pp.96-105
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    • 2010
  • Titanium implant is used as the most popular dental material for replacement of missing teeth recently. A lot of studies on the surface modification of titanium implant have been carried out for enhancing osseointegration. The surface modification techniques could be classified as follows; topographic modifications which provide roughness and porosity, chemical surface modificationss or deposition of osseoconductive materials, and biochemical modifications to immobilize bone growth factors on titanium surface. In this study, the current and ongoing surface modification techniques and its typical characteristics used in clinics were reviewed. In the future, study and implication about biochemical modifications including patient' s individual characteristics will be important.

High fructose and high fat diet increased bone volume of trabecular and cortical bone in growing female rats (고과당 및 고지방 식이의 섭취가 성장기 동물모델의 골성장과 골성숙에 미치는 영향)

  • Ahn, Hyejin;Yoo, SooYeon;Park, Yoo-Kyoung
    • Journal of Nutrition and Health
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    • v.48 no.5
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    • pp.381-389
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    • 2015
  • Purpose: The objective of this study was to investigate the effects of a high fructose and fat diet on bone growth and maturation in growing female rats. Methods: Three-week-old female SD rats were randomly assigned to four experimental groups; the control group (CON: fed control diet based on AIN-93G, n = 8); the high-fructose diet group (HFrc: fed control diet with 30% fructose, n = 8); the high-fat diet group (Hfat: fed control diet with 45 kcal% fat, n = 8); and the high-fat diet plus high fructose group (HFrc + HFat: fed diets 45 kcal% fat with 30% fructose, n = 8). Each group was assigned their respective diets for the remaining eight weeks. Bone-related parameters (bone mineral density (BMD) and structural parameters, osteocalcin (OC), deoxypyridinoline (DPD)) and morphologic changes of kidney were analyzed at the end of the experiment. Results: Final body weights and weight gain were higher in the HFat and HFrc + HFat groups and showed higher tendency in the HFrc group compared with those of the CON group (p < 0.05); however, no significant difference in caloric intake was observed among the four experimental groups. The serum OC levels of the HFrc and HFrc + HFat groups were lower than those of the CON and HFat groups (p < 0.05). Urinary levels of DPD did not differ among the experimental groups. BV/TV and Tb.N of trabecular bone were higher in the HFrc + HFat group and showed a higher tendency in the HFrc group than those of the CON and HFat groups (p < 0.05). Tb.Pf of trabecular bone were lower in the HFrc + HFat group than those in the CON and HFat groups (p < 0.05). However, no difference in trabecular BMD was observed among the experimental groups. Cortical bone volume was higher in the HFat and HFrc + HFat groups than in the CON and HFrc groups (p < 0.05). No morphology change in kidney was observed among the experimental groups. Conclusion: Our study suggests that 8 weeks of high-fructose and high fat intake could improve the bone quality (Structural parameters) of trabecular and cortical bone of tibia in growing female rats.