• Proceedings of the PSK Conference
• /
• 2003.10b
• /
• pp.226.2-226.2
• /
• 2003

.The healing of a bone defect is complex, and involves a wide range of cellular, molecular, physiological, and biological processes. The main effect of bone substitute is to promote wound healing by induce cell proliferation. Bone defect sites usually are localized below the original bone surface; therefore, space production and maintenance between the membrane and the original bone surface is essential. As a result, membranes must have proper mechanical strength to prevent the collapse of the soft tissue and maintain wound space that permits membranes of poly (L-lactide) (PLLA) were fabricated to provide and maintain sufficient space for bone growth. (omitted)

• Asian-Australasian Journal of Animal Sciences
• /
• v.16 no.1
• /
• pp.50-56
• /
• 2003

An experiment was carried out to compare the body weight, shank length, rectal temperature, comb area, abdominal fat, blood parameters and bone traits of capon, slip and intact birds in Taiwan country chicken cockerels. One hundred and sixty-six Taiwan country chicken cockerels were randomly assigned to caponized or intact male groups. Caponized birds were surgically altered at 10 weeks old and raised to 28 weeks old. At 28 weeks of age, the capons were separated into capon and slip groups, depending on the atrophy of the comb and wattle in size. The results showed that body weight and shank length in slips were significantly (p<0.05) greater than in intact birds. Intact birds had the highest (p<0.05）feather scores and the lowest (p<0.05) rectal temperature. Compared with intact birds and slips, capons had a significantly (p<0.05) smaller comb area. Bone percentage, stress and cortical thickness, and bone and ash calcium content and plasma testosterone concentration, in intact birds were the highest (p<0.05) followed by slips and capons. However, intact birds had the lowest (p<0.05) plasma ionized calcium and phosphorus concentrations. Bone and ash manganese contents in capons were significantly (p<0.05)lower than those in others. These findings support the hypothesis that androgenic effects on secondary sexual characteristics are stronger than anabolic growth promoting response. Androgens can directly influence calcium fluxes in male chickens. Caponized caused a reduction in the bone percentage, stress, cortical thickness and bone calcium content.

• Journal of Acupuncture Research
• /
• v.24 no.5
• /
• pp.13-22
• /
• 2007

Objectives : The differentiation of osteoblasts is controlled by various growth factors and matrix protein expressed in bone. The aim of this study was to investigate the effects of many herbs medicine(KHBJs) for bone healing that induces osteogenic activity in human osteoblast-like SaOS-2 cells. Methods : The osteogenic effects of KHBJs were evaluated by using cell proliferation(WST-8) assay, alkaline phosphatase(ALP) activity assay, colorimetric analysis of vascular endothelial growth factor(VEGF) expression in human osteoblast like SaOS-2 cell. Also, osteogenic activity of KHBJ fractions(KHBJB and KHBJR) by activity guided fractionation were evaluated. Results : About 7 KHBJs had effect on the proliferation of osteoblast like SaOS-2 cells, and dose-dependently increased alkaline phosphatase(ALP) activity. KHBJs markedly increased expression for VEGF. Fractionated KHBJs(KHBJB or KHBJR) not enhanced more than KHBJs on osteogenic activity in SaOS-2 cells. Conclusions: This study found that 7 KHBJs had effect on proliferation, ALP activity, and VEGF expression in osteoblast like SaOS-2 cells. These results propose that KHBJs can play an important role in osteoblastic bone formation, and may possibly lead to the development of bone-forming drugs.

• Maxillofacial Plastic and Reconstructive Surgery
• /
• v.13 no.1
• /
• pp.9-15
• /
• 1991

The cleft alveolus occurs about 75% of cleft lip and palate patients. The purpose of bone grafting is improve the maxillary growth, rehabilitation of continuty of maxillary arch and providing bone for periodontal support for unerupted teeth. The bone grafting for alveolar cleft defect repair are classsified; primary bone grafting, early secondary bone grafting secondary bone grafting and late secondary bone grafting. In this article, we reported the cases of PMCB grafts for repair of the alveolar clefts showed potential benifit to the patient to induce a normal maxillary growth and providing bone foor periodontal support of unerupted teeth.

• The Journal of Advanced Prosthodontics
• /
• v.2 no.1
• /
• pp.7-13
• /
• 2010

Although most researchers agree that platelet-rich plasma (PRP) is a good source of autogenous growth factors, its effect on bone regeneration is still controversial. The purpose of this study was to evaluate whether increasing angiogenic factors in the human PRP to enhance new bone formation through rapid angiogenesis. MATERIAL AND METHODS. In vitro, the human platelets were activated with application of shear stress, $20\;{\mu}g/ml$ collagen, 2 mM $CaCl_2$ and 10U thrombin/$1\;{\times}\;10^9$ platelets. Level of vascular endothelial growth factor (VEGF) and platelet microparticle (PMP) in the activated platelets were checked. In the animal study, human angiogenic factors-enriched PRP was tested in 28 athymic rat's cranial critical bone defects with $\beta$-TCP. Angiogenesis and osteogenesis were evaluated by laser Doppler perfusion imaging, histology, dual energy X-ray densinometry, and micro-computed tomography. RESULTS. In vitro, this human angiogenic factors-enriched PRP resulted in better cellular proliferation and osteogenic differentiation. In vivo, increasing angiogenic potential of the PRP showed significantly higher blood perfusion around the defect and enhanced new bone formation around acellular bone graft material. CONCLUSION. Angiogenic factor-enriched PRP leads to faster and more extensive new bone formation in the critical size bone defect. The results implicate that rapid angiogenesis in the initial healing period by PRP could be supposed as a way to overcome short term effect of the rapid angiogenesis.

• Journal of Periodontal and Implant Science
• /
• v.27 no.3
• /
• pp.647-668
• /
• 1997

6 beagle dogs aged over one and half years and weighed 14 to 16 Kg were utilized in this study, Horizontal furcation defects were induced around 2nd, 3rd, and 4th premolars bilaterally, PDGF-BB in conjunction with EGF and PDGF-BB only were applied in the right and left premolars respectively. 2 animals were sacrificed at 4weeks, 8 weeks, and 12 weeks, after regenerative surgery respectively. Semi-thin sections using glass-knife were stained with toluidine blue for light microscopic study. The results were as follows: 1. At 4 weeks after regenerative surgery, bone formation in the PDGF-BB-applied site was thriving, but bone formation in the PDGF-BB-and-EGF-applied site was depressed. 2. Bony ankylosis was surely shown along the whole exposed root surface applied with PDGF-BB, but it was shown at the root surface near the base of the bone defect where was applied with PDGF-BB in conjunction with EGF. 3. Active bone formation was made from 8 weeks after regenerative surgery in the PDGF-BB- and-EGF-applied site. 4. Bone maturity as well as speed of bone formation in the PDGF-BB-applied site was superior to those in the PDGF-BB-and-EGF-applied site throughout the whole experimental period. Within the above results, PDGF-BB had the strong capability to form the new bone and EGF was not able to prevent the bony ankylosis thoroughly. However, EGF may have the possibility to prevent the bony ankylosis through the suppression of bone formation.

• Journal of Oral Medicine and Pain
• /
• v.45 no.4
• /
• pp.83-88
• /
• 2020

Purpose: Growth differentiation factor 11 (GDF11) and myostatin (MSTN) are closely-related transforming growth factor β family members reported to play crucial roles in bone formation. We previously reported that, in contrast to MSTN, GDF11 promotes osteogenesis of vertebrae and limbs. GDF11 has been also reported as an important regulator in tooth development by inducing differentiation of pulp stem cells into odontoblasts for reparative dentin formation. The goal of this study was to investigate the differential roles of GDF11 and MSTN in dental and cranial bone formation. Methods: Micro-computed tomography analysis was performed on cranial bones, including frontal, parietal, and interparietal bones, and lower incisors of wild-type, Gdf11 knockout (Gdf11-/-), and Mstn knockout (Mstn-/-) mice. Tissue volume, thickness, and mineral density were evaluated for both cranial bone and lower incisors. Lower incisor lengths were also measured. Because Gdf11-/- mice die shortly after birth, analysis was performed on newborn (P0) mice. Results: Compared to those of Mstn-/- mice, cranial bone volume, thickness, and mineral density levels were all significantly diminished in Gdf11-/- mice. Tissue mineral density of Gdf11-/- mice were also significantly decreased compared to wild-type mice. Likewise, lower incisor length, tissue volume, thickness, and mineral density levels were all significantly reduced in Gdf11-/- mice compared to Mstn-/- mice. Incisor length was also significantly decreased in Gdf11-/- mice compared to wild-type mice. Mstn-/- mice exhibited mildly increased levels of tissue volume, thickness, and density in cranial bone and lower incisor compared to wild-type mice although statistically not significant. Conclusions: Our findings suggest that GDF11, unlike MSTN, endogenously promotes cranial bone and tooth development.

• Journal of Life Science
• /
• v.18 no.9
• /
• pp.1230-1238
• /
• 2008

The purposes of this study was to identify the effects of regular training on body composition, bone mineral density and growth factors by taekwondo training program in the children. Subjects were aged 11$\sim$13 years. There was classified two groups (experiment group 12, control group 12), total 24. Measured items for this study were body composition (weight, percent body fat, body mass index), lumbar (L2, L3, L4), femur (neck, trochanter, ward's triangles) bone mineral density and growth factors (growth hormone, IGF-I, IGFBP-III) in the 12weeks before and after. Results of this study were as follows; In the body composition, there were showed significant differences on the weight, percent body fat and body mass index level between groups. In the bone mineral density, there were showed significant differences on the lumbar 2, 3, and femur neck, femur trochanter, femur ward's triangles level between groups. In the growth factors, there were showed significant differences on the growth hormone, IGF-I, and IGFBP-III level between groups. This study showed that an 12weeks taekwondo training program affected in a positive effects in almost of items in trained children.

• The Journal of Pediatrics of Korean Medicine
• /
• v.22 no.2
• /
• pp.69-80
• /
• 2008

Objectives : The purpose of this study was to evaluate the correlation of 3 methods of bone age measurements. Methods : 102 children(49 boys, 53 girls) were involved in this study. We measured the height, weight and bone age based when they visited for the first time. We measured bone age using BoneAge of Sunlight Co.,Ltd. Two of the bone age measurement methods, Greulich-Pyle and Tanner-Whitehouse, were used to analyze the left dorsopalmar hand-wrist radiographs of 102 children who visited in Department of Pediatrics, O O University Oriental Hospital. This study was designed to investigate the correlation of the bone age between two methods. Results : The bone ages were related with age, height, weight and BMI according to this study(P<0.01). Each bone age assessment method had statistically significant correlation to each other(P<0.01). Conclusions : The ultrasound transonic velocity of inferior radiocarpal joint will become a sufficient diagnostic tool of bone age assessment if measurement error can be minimized by proper effort.

• Journal of Veterinary Clinics
• /
• v.31 no.5
• /
• pp.417-420
• /
• 2014

Bone grafts are essential for promoting bone healing in some orthopedic cases, and synthetic bone materials have been widely used for bone defects. In addition, osteoinductive proteins such as bone morphogenetic protein and fibroblast growth factor promote osteoblast differentiation and proliferation. The combination of these factors is very useful clinically for promoting bone healing. In this study, we report the use of synthetic bone materials and osteoinductive proteins to repair bone defects in two dogs.