• Title/Summary/Keyword: body dipping method

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Neuropharmacological study of hot water extract of the seeds of Vernonia anthelmintica Kuntze

  • Rahman, Mahbubur;Choudhuri, M Shahabuddin Kabir;Khan, Mahmud Tareq Hassan;Jabbar, Shaila;Alamgir, Mahiuddin
    • Advances in Traditional Medicine
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    • v.6 no.1
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    • pp.34-38
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    • 2006
  • The hot water extract of the seeds of Vernonia anthelmintica Kuntze (Compositae) in a dose of 10 ml/kg body weight of mice, showed significant analgesic activity on the hot plate analgesic method throughout the 4 h experimental period. The hole cross scores and the climbing out scores are of lower than the control animals. The hot water extract reduced the defecation in hole board study and significantly reduces the exploratory ambulation and head dipping behaviour. The seed also showed significant depressant activity on the exploratory ambulation of the open field scores. The drug decreased the spontaneous locomotion activity on brick-chip displacement method up to 45 min and also showed the ability to lessen the amphetamine induced hyperactivity up to 20 min. But it didn't show any effect on pentobarbital induced sleeping time test. The extract reduced gastrointestinal motility.

In vitro Uptake and Accumulation of Purified Storage Proteins into Fat Body Cells from Huphantria cunea Drury (생체밖에서 미국흰불나방 지방세포에 의한 저장단백질의 흡수와 축적에 관하여)

  • Lee, Bong-Hee;Kim, Kwan-Seon;Moon, Myung-Jin;Kim, Woo-Kap
    • Applied Microscopy
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    • v.18 no.2
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    • pp.102-118
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    • 1988
  • This study was carried out to examine in vitro first whether the storage proteins, which the fat bodies of last larvae from Hyphantria cunea secrete into haemolymph, can be uptaked by the fat body cells of prepupa and then how the uptaked storage proteins can be accumulated in the fat body cells, if uptaken. The fat bodies which had been isolated from last instar larvae were cultured in 1 ml of Grace's insect medium containing $50{\mu}l$ of $^{3}H$-leucine (5.0 mCi/mol, Dupont) at $28{\pm}2^{\circ}C$ for 6 hrs. After the homogenates of the cultured fat bodies were centrifuged at 10,000 rpm for 10 minutes, the proteins included in the supernatant were separated by polyacrylamide gel electrophoreses (non-SDS, 6%). The next treatment of the electrophoresed gel was followed by rinsing. A storage protein band of several bands in the rinsed gel was sliced off. With elution of sliced storage protein bands in Tris-glycine buffer, the purification of radioactive storage proteins from fat bodies was finished. After the purified radioactive storage proteins were added in Grace's insect midis containing fat bodies of the prepupae, they were cultured for the randomly following minutes given as 3, 5, 7, 10, 15, 20 and 30 and for the randomly following hours given as 1, 2, 3 and 4 respectively. The double fixations of the cultured fat bodies in aldehyde and $OsO_4$, were followed by preparation of ultrathin sections from Epon-Araldite blocks through dehydration and embedding. The electron microscope autoradiographic treatment of all prepared sections were performed by the dipping method (Kim et al., 1987). The finally prepared specimens were examined with electron microscope. The fat body cells of the prepupa could be found to uptake the storage preteins of the last instar larvae, which were included in the culture medium, mostly by formation of coated vesicles. The in vitro uptake of the storage proteins actively occurred by 30 minutes after the addition of purified storage proteins in the culture medium. After culture for 7 minutes with the storage proteins, the uptaked radioactive storage proteins labelled a number of lysosomal granules. After culture for 20 minutes with the storage proteins, the radioactive storage proteins were finally incorporated and accumulated in lipid droplets and protein granules. The frequency in the fat body cell of radiolabelled lipid droplets occurs approximately 60%, while the frequency, in which the radiolabelled protein granules occurs in a fat body cell, is approximately 40%.

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Susceptibility commercially of North American planthopper, Metcalfa pruinosa to commercially registered insecticides in Korea (국내시판 살충제에 대한 미국선녀벌레의 감수성)

  • Ahn, Ki-Su;Lee, Gwan-Seok;Lee, Kyeong-Hee;Song, Myung-Kyu;Lim, Sang-Cheol;Kim, Gil-Hah
    • The Korean Journal of Pesticide Science
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    • v.15 no.3
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    • pp.329-334
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    • 2011
  • Insecticidal activity of 31 registered insecticides was tested against Metcalfa pruinosa adults. All experiments were conducted at the recommended concentration (ppm) of each insecticide. Among them, 16 insecticides from organophosphates (dichlorvos, fenitrothion, fenthion, methidathion, phenthoate), carbamates (methomyl), pyrethroids (${\alpha}$-cypemethrin, deltamethrin, fenpropathrin, ${\gamma}$-cyhalothrin), neonicotinoids (acetamiprid, clothianidin, dinotefuran, imidacloprid, thiamethoxam), and other (endosulfan) showed 100% mortality by spraying on the body of M. pruinosa adults. Dichlorvos, fenitrothion, fenthion, methidathion, phenthoate and endosulfan showed 100% mortality by plant-dipping method. The residual effect was showed 100% mortality in four insecticides (fenitrothion, fenthion, methidathion, phenthoate) at one day after treatment, and three insecticides (fenitrothion, methidathion, phenthoate) were showed the mortality of 90% at three days after treatment.

Structural Stability, Weathering and Conservation Method of Granite Standing Sculptured Buddha at Hwangsang-dong, Kumi (구미 황상동 마애여래입상의 구조적 안정성, 풍화 및 보존방안)

  • Lee, Chan Hee;Choi, Suck Won;Suh, Mancheol;Chae, Sang Jeong
    • Journal of Conservation Science
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    • v.9 no.1
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    • pp.21-32
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    • 2000
  • Rock composition of the Hwangsang-dong Granite Standing Sculptured Buddha (Treasure No. 1122) in the Kumi City is biotite-hornblende granodiorite which consists of about 30 pieces of individual rock blocks of same compositions. However, the cap rocks is pebble-bearing coarse sandstone. Rock blocks of the Standing Buddha and surrounding out crops occur well developed several joint systems of $N25^{\circ}$ to $45^{\circ}W$ strike and nearly vertical (70 to $85^{\circ}SE$) dipping. Rock blocks of the Standing Buddha showed vertical, horizontal and oblique joints, and those blocks are well supported by individual blocks. However, the junction part of the blocks are under dangerous situation due 10 seriously mechanical and chemical weathering. Host rock of the Standing Buddha belongs to the HW grade, therefore mostly rock-forming minerals of the granodiorite Standing Buddha altered with clay and iron hydroxide minerals by mineralogical and chemical weathering. Near surface of the Standing Buddha show spore and mycelium of green algaes, and a joint plane alive with weeds. We suggest that if structural stability for the Standing Buddha remove essentially a unstable rock blocks from the main body, and the main body necessitate supporting by rock bolting method because of repeated unstability and minimizing stress to the rock blocks. For the opened joint planes, fractured surface and alive weeds will attempt to fill in a petro-epoxy, petro-filler and biochemical treatments for the algaes, and ground water curtain and wall seems to be necessary for water flow and diminishing humidity of the Standing Buddha.

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Toxicological and Electrophysiological Activities of Pyrethroids between Larvae of Diamondback Moth, plutella xylostella and Beet Armyworm, Spodoptera exigua (배추좀나방과 파밤나방의 pyrethroids약제에 대한 감수성 및 전기적 신경 반응 비교)

  • Ham, Sun-Hee;Ahn, Hee-Geun;Yang, Jeong-Oh;Yoon, Chang-Mann;Kim, Gil-Hah
    • The Korean Journal of Pesticide Science
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    • v.13 no.3
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    • pp.197-202
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    • 2009
  • Based on the insecticidal efficacy by insecticide treatment methods, neurophysiological responses were compared with the larvae of diamondback moth (DBM) and beet armyworm (BAW) using pyrethroids working on nervous system. By body spray method, all pyrethroids were showed lower insecticidal activity below 50% on the larvae of DBM and BAW. By leaf dipping method, DBM larvae were showed the insecticidal activity as 100% at 50 ppm deltamethrin, 80.0% at fenvalervate and 63.3% at permethrin. However, BAW larvae were showed lower insecticidal activity as similar as control in all concentration. In order to examine electrophysiological response on nervous system on DBM and BAW larvae when treated three insecticides, we investigated the voltage and reaction degree. The voltage of DBM were responded as high as $10^{-7}M$ in deltamethrin, $10^{-5}M$ in fenvalerate and $10^{-3}M$ in permethrin. However, those of BAW were showed dull responses with small variation of voltage to all three insecticides.

A Study on the Hot Springs(Tangsil Building) of Temporary Palace(Onyanghaenggung) according to the <Oncheonhaenggungdo>(1795) (<온천행궁도(溫泉行宮圖)>(1795)의 온천(탕실) 건축 고찰)

  • LEE Jeongsoo;KIM Ilhwan;LEE Kyeongmi;JI Wonku;CHOI Jaeseong
    • Korean Journal of Heritage: History & Science
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    • v.57 no.1
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    • pp.110-123
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    • 2024
  • Onyanghaenggung Palace(temporary palace at Onyang) is an important cultural heritage that can substantially confirm the king's visiting at hot springs based on literature records such as <Ongungyeonggoedae(溫宮靈槐臺)>, <Oncheonhaenggungdo(溫泉行宮圖)> of 『Ongungsasil(溫宮事實)』, <Younggoedaedo(靈槐臺圖)>, 『Younggoedaegi(靈槐臺記)』 and cultural properties such as Yeonggoedae(靈槐臺) and Shinjeong Monument(神井碑). Through a photo taken by Hermann Sander in 1906, it can be confirmed that the hot springs(Tangsil building) at Onyanghaenggung Palace during the Joseon Dynasty was maintained until the early Japanese colonial period. The purpose of this study is to estimate the compositions of the hot springs(Tangsil building) in Onyanghaenggung Palace based on literature records and <Oncheonhaenggungdo>(1795). To achieve these purposes, we firstly examined the changes in Onyanghaenggung Palace and the hot springs (Tangsil building); secondly, the bathing behaviors of kings were reviewed; thirdly, we organized the architectural composition of the hot springs (Tangsil building) according to "Ongung Repair" of 『Ongungsasil (溫宮事實)』; and fourthly, by comparing Sander's photo in the early days of Japanese colonial rule, the architectural composition of the hot springs (Tangsil building) in the late Joseon Dynasty was examined. The results of this study are as follows. First, the hot springs(Tangsil building) of Onyanghaenggung Palace were continuously connected to the Onjeongsil(溫井室) in the reign of King Hyeonjong and maintained until 『Hoseo-eupji』 (1871) in the late Joseon Dynasty. It matches the photograph taken by Hermann Sander(1906) and <1912 Onyang Hot Springs in Asan City>(1912) of Korea Copyright Commission during the early Japanese colonial period. Second, the various king's bathing methods during the Joseon Dynasty were adopted such as washing, spilling and bathing head while sitting on a bathing platform or chair, or exposing the steam of hot spring water, dipping feet into the water and a half-body soaking bath below the navel immersed in water. Third, the stone bathtubs of hot springs(Tangsil building) are composed of the upper bath which was hot spring water gushes out from the northwest, bends to the east, enters the middle bath, and bends to the south to come out to the outside to gather in the lower bath. Around the stone bathtubs, pebble stones brought in from Taean were laid on the floor of the hot springs(Tangsil building). From the above considerations, the compositions of the Tangsil building in Onyang Temporary Palace is based on the king's approach from the main royal building, the king's bathing method and bathing tools, the bathing behavior of enlisted medical officers and bathing assistants, and each rooms mentioned in "Ongung Repair". By comparing it with Hermann Sander's photo, the architectural compositions of the hot springs(Tangsil building) can be estimated.

A Land Resources Survey of the Mula Area, S. E. Spain (동남(東南)스페인 Mula지역(地域)에서의 Land Resources Survey)

  • Yun, Suckew
    • Economic and Environmental Geology
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    • v.6 no.1
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    • pp.29-64
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    • 1973
  • A land resources survey in the semi-arid area, Mula in S. E. Spain, of $400km^2$ is compiled. The basic aim of the project is to investigate the intrinsic qualities of the land resources of the area by means of applying an integrated method of natural resources survey mainly concerning with analysis and synthesis of land complexes, each of them represents an area or a group of areas with similar patterns of landforms, soils and vegetation, based on a geomorphological approach. The area is characterized by a linear arrangement of relief pattern with an asymmetric homoclinal repetition of slope attitudes elongating WSW-ENE, dipping steeply on the NW sides and gently on the SE sides, which have been resulted from the post-Alpine folding of the Triassic to Cretaceous limestone, the Eocene limestone, the Oligocene sandstone and the lower Miocene limestone and marl, and the post-lower Miocene faulting, tilting and subsequent differential erosion of the Miocene sedimentary formations. An integrated body of information in geology, landforms, soils and vegetation, which are significantry interrelated as an environmental complex, has been obtained. Using this data, 26 land complexes developing on the various situations of landforms, such as folded mountain ranges, tilted tablelands, bevelled cuestas, degraded hill-lands associating with enormous foots lopes, undulating terrains and terraced or flat plains, have been differentiated, mapped and described. The soils of the area are mostly light colored calcic lithosols which have been derived dominantly from the marly parent materials and developed into remarkable slope catenas in some places depending on the relief conditions. The land uses of the area are mainly characterized by the perennially irrigated cultivation of citrus orchards along the terraced alluvial deposits fringing the Segura and Mula River, and the dry-land cereal cultivation on gentler slopes. Pioneer dry-land cultivations within the shrubs on steeper slopes are restricted to the unchannelled tributary drainage floors. The availability of water is a fundamental controlling factor for existence of native and cultivated vegetation as a whole, and a number of active processes including sheet wash and gully erosion, especially on the scarp slopes, are the other important factors to be considered in conservation and management of the land in the area.

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Biokinetics of Carbohydrate and Lipid Metabolism in Normal Laying Hen [Part 1] -Determination of Turnover of Glucose- (정상산란계(正常産卵鷄)에 있어서 탄수화물(炭水化物)과 지질대사(脂質代謝)의 생동역학(生動力學) 제1보[第一報] -포도당 대사회전(代謝回轉)의 측정(測定)-)

  • Chiang, Y.H.;Riis, P.M.
    • Applied Biological Chemistry
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    • v.20 no.2
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    • pp.205-209
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    • 1977
  • The pool size of plasma glucose, turnover rate and other concerned items for glucose metabolism in normal laying hen were investigated by a single-injection method using $U-C^{14}-glucose$. The 11.6 nCi of pure dose was injected to a hen normally fed through the wing vein. The glucose concentration in plasma sample taken at 5 minutes after injections was 214mgper 100ml. From the plottings of logarithmic standard specific activities of plasma taken from 5 to 120 minutes against the time after injection and from the regresion analysis, metabolic states were determined. The pool size was 1.07g, turnover rate was 0.024 per minute, turnover time was 41 minutes, utilization rate was 26mg/min. (0.83 g/hr/kg B.W. 3/4) and glucose space(extracellular fluid volume) was 25.3 per cent of body weight. The values obtained from. 10-50 minutes samples were similar to those described above, which we from 5-120 minutes samples.

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