• Journal of Acupuncture Research
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• v.20 no.2
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• pp.135-144
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• 2003

Objective : The purpose of this study is To investigate the effects of acupuncture and moxibustion at Zusanli(ST36) and Weishu(BL21) Methods : serum gastrin level by radioimmunoassay was measured at 5 days after acupuncture and moxibustion of those acupoints. Gastric endocrine cell(G cell and Histamine immunoreactive density) by avidin-biotinylated complex(ABC) technique, histological examinations(Alcian Blue-PAS Stain; Alcian blue-Periodic Acid Schiff reagent) of the gastric mucosa were also performed. Acupuncture applied to the ST36 acupoint increased gastrin level of serum, but moxibustion did not produced significant effect. All of acupuncture and moxibustion at BL21 acupoint increased gastrin level of serum significantly. In moxibustion at ST36 and BL21, the number of gastrin secreting cells in gastric mucosa, the density of immunoreactive histamin secreting cells and the density of body mucosa stained by PAS were decreased compare to acupuncture at ST36 and BL21. In acupuncture and moxibustion at BL21, the density of pylorus mucosa stained by PAS were increased compare to the groups applied to ST36. In the density of body mucosa stained by AB, moxibustion at BL21 and ST36 were increased compare to the other groups. Results : These data suggest that acupuncture and moxibustion at BL21 increased gastrin level of serum and those effects were more potent than acupuncture at ST36.

• Journal of Mushroom
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• v.10 no.1
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• pp.44-48
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• 2012

In this study, whether Giemsa staining solution can accurately determine bacterial contamination of liquid spawn for Flammulina velutipes in a short period of time was investigated. Giemsa solution staining cells of blood, bone marrow, lymph node, malaria parasites, rickettsia et al. was prepared by dissolving basic methylene azul and methylene blue, and acidic eosine in methyl alcohol-glycerine. Supernatant samples of Flammulina velutipes liquid spawn cultured under explosive aeration were placed on a slide, mixed with Gimesa solution and examined with optical microscope after staining. In 40 to 60 seconds bacterial cells were distinguishable from soybean meal residual and hyphal cell fragments. Thus we conclude that microscopy using Gimesa staining solution is a quick, simple and accurate method for the mushroom growers to effectively use to detect bacterial contamination of the liquid spawn.

• Applied Microscopy
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• v.51
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• pp.5.1-5.7
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• 2021

The olfactory organs of two African lungfishes, Protopterus amphibius and P. dolloi, were investigated using a stereo microscope and a compound light microscope and were described anatomically, histologically, and histochemically. Like other lungfishes, these species present the following general features: i) elongated olfactory chamber (OC), ii) anterior nostril at the ventral tip of the upper lip, iii) posterior nostril on the palate of the oral cavity, iv) lamellae with multiple cell types such as olfactory receptor neurons, supporting cells, basal cells, lymphatic cells, and mucous cells (MC), and vi) vomero-like epithelial crypt (VEC) made of glandular epithelium (GE) and crypt sensory epithelium. Some of these features exhibit differences between species: MCs are abundant in both the lamellar and inner walls of the OC in P. amphibius but occur only in lamellae in P. dolloi. On the other hand, some between feature differences are consistent across species: the GE of both P. amphibius and P. dolloi is strongly positive for Alcian blue (pH 2.5)-periodic acid Schiff (deep violet coloration), and positive with hematoxylin and eosin and with Masson's trichrome (reddish-brown staining), unlike the MCs of the two species which stain dark red with both Alcian blue (pH 2.5)-periodic acid Schiff and Masson's trichrome but respond faintly to hematoxylin and eosin. The differing abundance of MCs in the two lungfishes might reflect different degrees in aerial exposure of the olfactory organ, while the neutral and acid mucopolysaccharide-containing VEC, as indicated by staining properties of the MCs, is evolutionary evidence that P. amphibius and P. dolloi are the closest living relatives to tetrapods, at least in the order Dipnoi.

• The Korean Journal of Malacology
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• v.24 no.3
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• pp.229-241
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• 2008

Changes of growth and histopathological feature in the mantle structure of the equilateral venus, Gomphina veneriformis exposed to tribultyltin chloride (TBTCl) for 36 weeks were observed. Concentrations of TBTCl were 0, 0.4, 0.6, and $0.8{\mu}g/L$. A regression analysis by power function of SPSS was shown that the growth of experimental groups was significantly decreased after 12 weeks of exposure. For histological analysis, mantle tissues were characterized using H-E stain, AB-PAS (pH 2.5) reaction and Masson's trichrome stain, and epidermal layer thickness and mucous cell distribution were analysed using the image analyser. The mantle had 4-folds (inner-inner, inner-outer, middle, and outer) and its epidermal layer consisted of simple epithlia. A periostracum was observed in the periostracal groove between middle and outer fold. Inner epidermal layer consisted of simple ciliated columnar epithelia, but the outer epidermal layer consisted of simple non-ciliated columnar epithelia. Alcian blue positive mucous cells showed blue color (7462c, 653c) in the inner fold, violet color (2583c) in the middle fold, and blue color (647c, 7455c) in inner epidermal layer (numbers in the parenthesis are codes of Pantone process coated color). Hemolymph sinus in the mantle was extended, and mucous cells in inner plica of the middle fold were stained as blue (7455c) and violet (2587c), after 12 weeks of TBTCI exposure. Cilia and striated border were disappeared, and number of mucous cells in the inner epidermal layer was reduced. Serious histopathological changes in middle and outer fold near the periostracum were observed after 36 weeks. Moreover, epidermal layer thickness and mucous cell distribution were showed decreasing tendency as exposure time to TBTCI was increased. Results of this study suggested that TBTCl induced growth disorder with histopathological changes.

• Journal of Veterinary Clinics
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• v.24 no.4
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• pp.644-646
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• 2007

This report deals with a 9-year-old neutered male Yorkshire terrier that had been suffered from vomiting, bloody diarrhea, anorexia. On exploratory laparotomy, pale-pink $10{\times}6cm$ duodenal mass was found and submitted to the Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University. Histologically, the duodenal mass consisted of compact sheet of poorly demarcated, highly infiltrative neoplasm. The neoplastic cells were round to polygonal and contained scant to moderate amounts of granular basophilic cytoplasm and eccentrically located irregularly round nuclei with stippled chromatins. The neoplastic cells were positive to lamda light chain immunohistochemically but were uniformly negative for toluidine blue stain and giemsa stains. Based on these results, this case was diagnosed as primary extramedullary plasma cell tumor of duodenum.

• Korean Journal of Veterinary Research
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• v.27 no.2
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• pp.347-359
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• 1987

To investigate the changes of pituitary gland associated with ovarian cyst in Korean native cattle, pituitaries and ovaries were collected from 54 Korean native cattle at abbatoir. Pituitaries were stained with HerIant pituitary stain method and all the tissues were examined under light microscope. The results obtained were summarized as follows; 1. The delta cells and beta cells in the pars distalis were dull blue and violet in colors respeetively. Basophil size in the follicular phase and pregnant groups was larger than those of luteal phase and ovarian dysfunction groups. 2. The numbers of delta cells in the pars distalis of follicular cyst group were larger than those of remaining groups (p<0.01). 3. The distribution of delta cells in the acidophil zone was greater than that in the basophil zone of the follicular cyst and follicular phase groups. 4. The granulations of delta cells were more intensive in follicular cyst, follicular phase and pregnancy groups than in luteal phase and ovarian dysfunction groups (p<0.01). 5. The numbers of beta cells in follicular phase and ovarian dysfunction groups were larger than those in luteal phase and follicular cyst groups respectively (p<0.01). 6. In all groups, the distribution of beta cells was greater in basophilic zone than in acidophilic zone (p<0.01).

• The Korean Journal of Cytopathology
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• v.8 no.1
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• pp.93-97
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• 1997

A case of cervical chordoma diagnosed by fine needle aspiration is discussed. A 41year-old male was admitted due to dyspnea on neck flexion. Radiologic image revealed a retrotracheal superior mediastinal solid mass. Aspiration cytology showed many clusters of oval or large polygonal cells having abundant eosinophilic or bubbly cytoplasm in an amorphous blue-gray mucoid background. The nuclei were round and showed size variation, coarse granular chromatin, and indistinct nucleoli. Some cells contained brown granular pigments in the cytoplasm. Mitoses were rarely found. The cytoplasm was strongly positive for PAS stain. Immunohistochemical stains using cell block revealed positive reaction for cytokerain, EMA, vimentin, and S-100 protein. The confirmative diagnosis was made by following excisional biopsy. Electron microscopic study revealed large pools of intracytoplasmic glycogen and microfilaments. This is the first case of cervical chordoma diagnosed by aspiration cytology to our knowledge in Korean literature.

• Journal of the Korea Furniture Society
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• v.25 no.4
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• pp.319-324
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• 2014

Red pine is a popular species for making a cutting board in Korea, but easily sap stained. Heat treatment could improve its quality by darkening and equalizing the color of sap stained wood. The color change of sap stained red pine boards was investigated by heat treatment at $190^{\circ}C$. It was observed that the color of heat treated boards got darker and it made the color of sap stain vanished. A colorimeter was used to measure color indexes. It was revealed that the values of the lightness ($L^*$) and the yellowness ($b^*$) decreased as heat treatment repeated while the values of the redness ($a^*$) increased. The average of the color difference (${\Delta}E^*$) between the control and 1st heat treated boards was 16.1, which could be expressed as 'Extremely different' while that between the 1st and 2nd heat treated boards was 8.3, which could be expressed as 'Considerably different'. The fact that heat treatment equalized the color of boards was confirmed by a statistical analysis.

• Textile Coloration and Finishing
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• v.6 no.2
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• pp.30-39
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• 1994

To study the printing behaviors of Polyester and cotton(P/C) fabrics printed with disperse and reactive dyes, the effects of alkalis on the fixation of reactive dyes and the alkali-stability of disperse dyes in various methods of fixation were examined. The anthraquinone disperse dyes which have diamino derivatives as substituents without hydroxy group, such as C.I. Disperse Violet 1(D.V.1), C.I. Disperse Violet 28(D.V.28) and C.I.Disperse Blue 60(D.B.60) showed good results of fixation without regard to the concentration of NaHCO$_3$. In case of high temperature steaming(HTS) and unsaturated steaming(US)/HTS, D.V. 1 was alkali-stable and effective for P/C printing. A good result was obtained with D.V.1 and C.I.Reactive Orange 13(R.O.13) paste of 4% $K_{2}CO_{3}$. It was found that the unfixed D.V.28 bearing chloro group can hinder the fixation of monochlorotriaxinyl reactive dyes, and D.B.60 made little stain on 100% cotton. In thermosol(Tm), the dye uptake of D.V.1 was not decreased so much, but those of D.V.28 and D.B.60 were greatly decreased.

• Korean Journal of Pharmacognosy
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• v.19 no.2
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• pp.133-140
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• 1988

The effect of ethanol extracts of Gilkyung(Platicodi Radix, Platicodongrandiflorum A, DC), Onji(Polygalae Radix, Polygala tennuifolia Willdenow) and Deoduk(Codonopsis lanceolate Radix, Codonopsis lanceolata) on expectorant activity of rat trachea was investigated. Following treatment of 50% ethanol extract of these medicinal plants (25 mg/rat), the content of neurotransmitters such as acetylcholine and histamine in tissue was significantly increased. The secretions of acid glycoproteins and the artificially injected phenol red were also increased. However, there was no significant difference except Onji From the histological study through periodic acid Schiff and alcian blue stain, the thickness of inner membrane of acinar glands and the stained glycoproteins on surface of epithelium and on the glands were observed in all the rats trachea treated with extract of medicinal plants. In vitro, the viscosity of mucin solution was slightly decreased with an addition of the extracts. Onji showed the most effective expectorant activity among them at the identical conditions. The mechanism of expectorant activity of these medicinal plants seems to be due to stimulation of secretion and changes of rheological properties of mucus.