• 한국응용과학기술학회지
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• 제26권1호
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• pp.51-59
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• 2009

Natural Artemisia extraction was extracted from Artemisia component using diethyl ether as a solvent, and we tested various pharmacetical and chemical characteristics of this extract. Characteristic experiments to use natural Artemisia extract tested antimicrobial experiment using microbe in pharmacetical material, and tested dye experiment using fiber in chemical material. From the result of characteristics experiment, some conclusions are obtained as follow. From the result of extraction experiment, it obtained about 10.4%-Artemisia extraction ratio as semi-solid state, and after dried in freezing from Artemisia extract of semi-solid state, it obtained about 10%-Artemisia extraction ratio as solid state of dark blue-green color. From result of antimicrobial experiment of Artemisia extract, number of staphylococcus aureus (ATCC-01) and aspergillus niger (ATCC-02) in microbe decreased more and more according to time passage. This phenomenon showed that Artemisia extract influences to antimicrobial effect. From the result of dye experiment of Artemisia extract, it appeared in direction of dark blue-green color after dyed to use cotton and silk with fiber to control in pH 7.5. Specially the result which confirmed dye of fiber with optical electron microscope (OEM), we could know that it appears darker silk than cotton.

• 한국균학회지
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• 제42권3호
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• pp.213-218
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• 2014

높은 laccase 활성을 보유한 큰느타리 수확 후 배지추출물(SMSE)은 bromophenol blue과 remazol brillient blue R염료에서 각각 93.7%와 88.7%의 높은 탈색효과를 나타내었다. 그 외의 Congo red는 72.13%을 보인 반면에 다른 염료는 60% 내의 탈색효과를 나타내었다. 특히 직물염색에 사용되는 Rit (red)와 Rit (blue)는 51.6%와 30.4%의 비교적 낮은 탈색율을 보였으며 Methylen blue 염료는 탈색률이 검출되지 않았다. 염료는 $20{\sim}30^{\circ}C$의 온도에서 가장 높은 탈색률을 보였고, $50^{\circ}C$까지 높은 염료 탈색효과를 보였다. 시간에 따른 염료 탈색효과 실험에서 bromophenol blue와 remazol brillient blue R은 SMSE 처리 1시간 후에 반응이 나타나기 시작하여 12시간 후에는 80% 이상의 높은 탈색율을 보였으나 다른 염료들은 탈색 정도가 서서히 증가되어 24시간 후 최고 60%의 탈색율을 보였다. 큰느타리 SMSE는 섬유공장으로부터 수집한 산업 폐 염료의 탈색효과를 나타내어 환경친화적인 염료탈색의 산업적 이용성을 제시하였다.

• 한국염색가공학회지
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• 제22권2호
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• pp.145-154
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• 2010

An alkalophilic microorganism capable of degrading dyes was developed for the treatment of alkaline dye solution. This strain was identified as Pseudomonas species. Using this microorganism, biological treatment of dye was studied in Erlenmeyer flasks. The characteristics of this microorganism were observed under various incubating-condition such as temperature, pH, nitrogen source, and macronutrients concentration. The removal effciencies of Disperse Red 60 from synthetic wastewater were 33.5 ~ 36.9% at the range of $30{\sim}40^{\circ}C$, and they were 31.1 ~ 36.7% at the range of initial pH 8 ~ pH 10, respectively. The optimal culture medium was found to be 0.25%(w/v) yeast extract, 0.25%(w/v) polypeptone, 0.1%(w/v) $KH_2PO_4$, 0.2%(w/v) $MgSO_4{\cdot}7H_2O$, and 1.0%(w/v) $Na_2CO_3$. In treatment of various dyes using Erlenmeyer flasks, the removal effciencies of Disperse Blue 87, Disperse Yellow 64, Disperse Red 60, Acid Blue 193, Acid Red 138, and Direct Yellow 23 were found to be 76%, 71%, 58%, 93%, 94%, and 90% respectively after 24hrs reaction of alkalophilic strain Pseudomonas sp. YBE-12.

• 한국약용작물학회지
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• 제21권3호
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• pp.213-219
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• 2013

This study was carried out to find out the optimum method of preparation of indigo standard solution and its stability, and to investigate the indigo contents in Niram, blue dye extract, from a total of 7 indigo plants and 34 breeding lines of Persicaria tinctoria H. Gross. Proper solvent for indigo standard was dimethyl sulfoxide (DMSO), and appropriate concentration was 1 mg of indigo in 10 mL of DMSO. Absorbance value of UV/Vis Spectrophotometer at 620 nm of standard solution was changed decreasingly 12 hours after the preparation of standard solution irrespective of the storage conditions such as temperature and light. Average value of absorbance of 8-fold diluted standard solutions prepared daily during 16 days was $0.210{\pm}0.005$, indicating the powder of indigo compound was stable chemically. Calibration curve was made for quantitative analysis of indigo of 7 Niram samples, and indigo contents ranged from 0.69% to 18.76% showing relatively larger variation. Across all 34 breeding lines, the range of indigo content was from 7.9 mg to 56.4 mg per 100 g of fresh leaves, averaging 25.2 mg of indigo content and showing a 47.7% coefficient of variation.

• Korean Chemical Engineering Research
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• 제61권2호
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• pp.287-295
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• 2023

Iron nanoparticles (Fe-NPs) were synthesized employing Lagenaria siceraria (LS) leaf aqueous extract as a reducing and capping medium to remove methylene blue (MB) dye and have antibacterial properties against G-negative (Escherichia coli) and G-positive bacteria (Staphylococcus aureus). The formation of LS-Fe-NPs (Lagenaria-siceraria-iron-nanoparticles) was confirmed by a change in color from pale yellow to dark brown. Characterization techniques, such as particle size analysis (PSA), transmission electron microscopy (TEM) and scanning electron microscopy (SEM), were employed to prove nano spherical particles of size range between 80-100 nm. Phytochemicals and the presence of iron in LS-Fe-NPs nanoparticles were proved by UV-visible spectrophotometry. Further, Fourier transform infrared spectroscopy (FTIR) analysis results confirmed the existence of bioactive molecules in the plants. The magnetic property was analyzed using a vibrating sample magnetometer (VSM), which displayed that the synthesized nanoparticles were superparamagnetic and exhibiting a saturation magnetization of 12.5 emu/g. Synthesized magnetic nanoparticles were used in methylene blue (MB) dye removal through adsorption. About 83% of 100 mg/L MB dye was removed within 120 min at pH 6 with a maximum adsorption capacity of 246.8 mg/g. Antibacterial efficacy of LS-Fe-NPs was screened against G-negative (Escherichia coli) and G-positive bacteria (Staphylococcus aureus), respectively, and found that LS-Fe-NPs were effective against Staphylococcus aureus.

• 복식문화연구
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• 제19권3호
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• pp.612-621
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• 2011

The process of making or cultivating indigo dyes is very cumbersome and complex. The dye extraction and dyeing methods using general plant dye, moth repellent dye, fast acting natural dye, and other dyes are very different. This research investigates the extraction of indigo dye and liquid dye extraction of polygonum(indigo) plants using calcium oxide water. While extracting indigo dye the concentration of purified indigo dye may be controlled by adjusting the pH level. Due to the various uses of dyes the adjustment of surface color must be considered. In regard to the change according to different concentrations of reducing agents, it was found that cotton fabrics and ramie fabrics show the highest color difference at 0.4% and 0.3% respectively. As the reduction temperature increases, the color difference increases as well. The maximum color difference was found to appear at $90^{\circ}C$. Cotton fabrics and ramie fabrics showed 70.55 and 67.01 respectively. The color difference increases as the concentration of dyes increases, but at a concentration of 300%, cotton fabrics was found to show 6.22PB in H value using the Munsell color system, containing purple and blue color. The pH of the polygonum dyes extracted through this experiment were adjusted by adding calcium oxide to the experimental water, without directly adding calcium oxide to the liquid polygonum extract. In a refine state, it was mixed with polygonum extract to extract a more refine and highly concentrated indigo dye. When lye and reducing agents are added to extracted indigo dye and sealed for long-term storage, it can be effective and easily used for dyeing.

• 생약학회지
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• 제15권4호
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• pp.203-205
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• 1984

The extract of Ssangkum-tang was investigated on antiinflammatory activities. The extract showed antiedematous action at a dose of 500mg/kg po in carrageenin edema test in rats. The extract also inhibited the leakage of pontamine sky blue into peritoneal cavity in mice at the doses of 300 and 500mg/kg po.

• KSBB Journal
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• 제19권3호
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• pp.200-205
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• 2004

반월공단의 염색폐수와 염료폐수를 균원시료로 하여 반응성 염료의 색도제거능이 나타난 13종의 균주를 분리하고, 산소농도에 따른 영향을 살펴보기 위하여 교반에 따른 색도제거율을 살펴 본 결과, 교반을 진행하지 않은 경우 효율이 더 높은 효율을 얻을 수 있었다. 또한, 5가지 반응성 염료 (reactive blue 19, reactive blue 21, reactive red 180, reactive red 195, reactive yellow 145)를 가지고 색도제거 실험을 진행한 결과 2종의 균주가 높은 효율을 보였다. 이 2종의 균주를 동정한 결과 Bacillus anthracis와 Bacillus cereus로 판명되었다. 최적 조건을 살펴보기 위하여 온도와 초기 pH 영향에 따른 색도 제거율을 알아보기 위하여 실험을 진행한 결과 35$^{\circ}C$, pH 7에서 높은 색도제거율을 보였다. 또한 탄소원과 질소원에 따른 영향을 살펴보기 위하여 Bacillus anthracis로 실험한 결과 glucose, yeast extract를 사용한 경우 89%의 높은 색도 제거율을 얻을 수 있었다. 이러한 결과로 미루어 볼 때 분리된 두 균주는 염색폐수의 색도제거에 효과적인 균주라고 사료된다.

• Journal of Pharmaceutical Investigation
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• 제4권1_2호
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• pp.46-54
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• 1974

A new spectrophotemetric method was estalished for the determination of oleic acid. This method is based on the 1,2-dichloroe-thane extraction of the ion-pair formed between methylene blue and oleic acid. But the absorbance of an ion-pair in the 1,2-dichloroe-thane layer was variable with the temperature, the ion-pair was extracted back into a diluted hydrochloric acid solution. The maximum absorbance of the acid extract observed at $660m{\mu}$ and a lineal relationship was observed from the initial amount over the range of $50-800{\gamma}/ml$ of oleic acid in the aqueous phase. The composition ratio of the ion-pair formed between methylene blue and oleic acid was determined to be 2 : 1 by both the mole ratio and continuous variation methods.

• 한국균학회지
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• 제39권1호
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• pp.48-52
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• 2011

본 연구에서는 표고 교잡균주들의 생화학적 특성을 이해하기 위한 하나의 시도로서 교잡균주간의 laccase 효소의 활성을 비교하는 빠르고 간편한 방법을 모색하고자 균사체 배양 배지의 성상과 염색물질이 laccase 활성 검출에 미치는 영향을 조사 비교하였다. 고체배지와 액체배지 모두에서 발색시약이 Remazol Brilliant Blue R(RBBR)일 경우는 potato dextrose 성분 보다 malt extract 성분 배지를 사용하는 것이 표고버섯균 균사가 분비하는 세포외 laccase 활성 검출이 용이하였다. Guaiacol을 발색시약으로 사용하는 경우는 potato dextrose 성분과 malt extract 성분 배지 모두 세포외 laccase 활성 검출이 용이하였다. 2ml microfuge tube를 이용한 액체 배양 방법은 RBBR와 Guaiacol 모두 malt extract 성분배지에서 3일만에 표고 교잡균주간의 세포외 laccase 활성 검출과 정량적 비교를 가능케 하였다.