• Proceedings of the Korean Society of Broadcast Engineers Conference
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• 1998.06b
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• pp.16.2-21
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• 1998

Old movies are often corrupted by randomly located blotches and scratches. In this paper were present an efficient method for detection and removal of these distortions. The presented method is composed of two separate steps: the detection process and the restoration process. In the detection process, blotch locations are detected through global motion segmentation, the sequential approach to motion segmentation, a robust model-fit criterion and so on, we form the algorithm for the algorithm for the global motion segmentation tuned to the blotch detection problem. In the restoration process, the missing data of the detected blotch areas are temporally extrapolated from the corresponding image areas at the preceding or the succeeding image frame with considering the global motion segmentation results. We apply the presented method to moving image sequences distorted by artificial blotches. The method works very well and provides a subjective improvement of picture quality.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.46 no.2
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• pp.1-9
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• 2009

In recent years, a film restoration has gained increasing attention by many researchers, to support multimedia service of high quality. In general, an old film is degraded by dust, scratch, flick, and so on. Among these, the common factors are scratch and blotch, so that many researchers have been investigated to restore these degradations. However, the methods in literature have one major limitation: A method is working well in dealing with scratches, however it is poorly working in processing the blotches. The goal of this work is to develop a robust technique to restore images degraded by both scratches and blotches. For this, we use MRF-MAP (Markov random field - maximum a posteriori) framework, so that the restoration problem is considered as the minimization problem of the posteriori energy function. As the minimization is one of complex combinatorial problem, we use distributed genetic algorithms (DGAs) that effectively deal with combinatorial problems. To asses the validity of the proposed method, it was tested on natural old films and artificially degraded films, and the results were compared with other methods. Then, the results show that the proposed method is superior to other methods.

• Applied Biological Chemistry
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• v.49 no.4
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• pp.281-286
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• 2006

Tolaasin, a pore-forming toxin, is a 1,985 Da peptide produced by Pseudomonas tolaasii and causes a brown blotch disease on cultivated mushrooms. Tolaasin forms pores on the plasma membrane of various cells including fungi, bacteria, plant as well as erythrocytes, and destroys cell structure. $Zn^{+2}$ has been known to block the tolaasin activity by an unknown mechanism. Thus, we investigated the inhibitory effects of $Zn^{+2}$ on the tolaasin-induced hemolysis to understand the molecular mechanism of tolaasin-induced pore formation. $Zn^{+2}$ and $Cd^{+2}$ inhibited the tolaasin-induced hemolysis in a dose-dependent manner and their Ki values were 170 ${\mu}M$ and 20 mM, respectively. The effect of $Zn^{+2}$ was reversible since the subsequent addition of EDTA chelates $Zn^{+2}$ and removes the inhibitory effect of $Zn^{+2}$. When an osmotic protectant, PEG 2000, was added, the tolaasin-induced hemolysis was not observed. After the removal of osmotic protectant by centrifugation, resuspended erythrocytes with fresh medium were immediately hemolyzed, while the addition of $Zn^{+2}$ prevented from hemolysis, implying that tolaasin-induced pores on the membrane were already formed in the medium containing osmotic protectant. These results suggest that $Zn^{+2}$ inhibits the activity of tolaasin pores and it has minor effects on the membrane binding of tolaasin and the formation of pore.