• The Korean Journal of Physiology
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• 제2권2호
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• pp.93-99
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• 1968

The Present study attempted to analyze the fate of CO diffused into the circulating blood through the alveoli. Dogs were induced to CO poisoning by rebreathing CO gas mixture contained in Krog's spirometer, by closed circuit method, for 60 minutes. The spirometer was filled initially with 282 ml of CO and 20 liters of air and oxygen, so the composition of gases were arranged as 1.4% in CO and 50% in $O_2$ at the begining of the rebreathing. Oxygen was added corresponding to the utilization of $O_2$ by the animal in proceeding of the experiment. At 60th minutes of CO rebreathing, the concentration of CO in arterial blood and in mixed venous blood were analysed and compared with each other after the CO contents were corrected with the hematocrit measured in the arterial and mixed venous blood. The distribution of CO gas to other tissues was estimated by the analysis of CO diffused into the cystic bile and into the peritoneal gas pocket which was formed by injection of 300 ml air into the peritoneal cavity prior to the CO gas rebreathing. The blood volume was measured by dilution method using $^{51}Chromium$ tagged red cells. CO amount vanished in the animal body was calculated by subtraction of total CO content in blood stream and the CO remained in closed circuit breathing system from the CO amount given to the breathing system at the begining of the experiment. Results obtained are summarized as follows: 1. The content of CO corrected by the hematocrit value was slightly less in mixed venous blood than in arterial blood. The amount of CO diffused into the cystic bile and into the peritoneal cavity was averaged to 0.1% and 0.4% of the CO amount in 100 ml of blood, respectively. 2. For 60 minutes of CO rebreathing, CO-hemoglobin saturation reached about 77% at the 60th minutes, CO amount vanished in the experimental animal averaged 36.1 ml/dog/hr., or 21% of the total CO volume in the blood stream. The average vanishing rate of CO during 60 minutes of CO rebreathing per kg of body weight was 2.71 ml/hr. Production of CO measured in ten dogs under hypoxic condition averaged 0.023 ml/kg/hr. The major part of the CO vanished in the dogs seemed to be oxidized to $CO_2$ by various tissues of the animal. The conclusion might be delivered as such oxidation of CO to $CO_2$ by animal tissues can play a role in part of the process of recovery and protection of animal from CO-poisoning.

• 약학회지
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• 제54권6호
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• pp.429-440
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• 2010

HS-SPME-GC/MS was studied and optimized for the determination of 17 orgarnophosphorous pesiticides (OPPs: chlorpyrifos, chlorpyrifos-methyl, demeton-s-methyl, diazinon, dimethoate, EPN, fenitrothion, fenthion, malathion, methidathion, monocrotophos, parathion, phenthoate, phosphamidon, sulfotep, terbufos, triazophos) in blood. Optimum SPME parameters were selected: choice of SPME fiber (85 ${\mu}m$ polyacrylate), pH effect (0.5 N HCl), salt effect ($Na_2SO_4$, 0.2 g; 20%), headspace incubation temperature ($80^{\circ}C$), headspace incubation time (1 min), headspace adsorption time (30 min) and GC desorption time (2 min). These parameters were optimized using HS-SPME autosampler coupled with gas chromatography-mass spectrometry (GC-MS). Method validation was carried out in terms of linearity, limit of detection (LOD), limit of quantitation (LOQ) and recovery in blood. The assay was linear over 0.5~5.0 mg/l ($r^2$=0.955~1.000). Limit of detection (LOD) and limit of quantitation (LOQ) in blood were determined 0.03~0.3 mg/l (S/N=3) and 0.1~1.1 mg/l (S/N=10), respectively. Relative recovery with 0.5, 1 and 5 mg/l (in blood) were 90.8%, 98.5% and 94.1%, respectively. This method will be applied to the determination of the orgarnophosphorous pesticides in postmortem blood. The proposed protocol can be an attractive alternative to be used in routine toxicological analysis.

• Journal of Chest Surgery
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• 제27권10호
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• pp.833-837
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• 1994

Lung contusion due to blunt chest trauma is the most common lung injury and correlated with the clinical course and prognosis. Its diagnosis by CT[Computerized Tomogram] gives a more clear and understandable three dimensional view, by which we are able to measure the volume of the contused and entire lung. Other variables are arterial blood gas, number of rib fracture, presence of hemopneumothorax, sternal fracture and clavicle fracture, number of associated non-thoracic injuries, ventilator time and presence of pulmonary complication. Percentage[%] of lung contusion are expressed as mean $\pm$ standard deviation and data analysis was performed by means of multivariate repeated measures analysis of variance to detect significant differences in variables between positive thoracic injury group and negative group. The paired t-test was used. Differences of percentage of lung contusion between groups were assessed by one-way analysis of variance. Simple linear regression was used to perform correlation analysis in the number of rib fracture and ventilator time. A p value less than 0.05 was considered statistically significant. Pneumothorax and the number of associated other injuries affect the amount of lung contusion and pulmonary complication group has more contused lung volume. Arterial blood gas study shows no correlation with the amount of lung contusion statistically. The number of rib fracture correlated with the amount of lung contusion, which also correlated with ventilator time[r=0.56, p<0.05]. In conclusion, quantitative anlysis of lung contusion by CT predicts the clinical course and treatment such as ventilator care.

• 한국임상수의학회지
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• 제32권6호
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• pp.499-503
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• 2015

본 연구의 목적은 건강한 한우 송아지의 동맥과 정맥혈액의 혈액가스, 전해질, 생화학 및 혈액학적 측정치를 조사하는 것이다. 건강한 3주령 이내의 62두 송아지를 본 연구에 공시하였으며 동맥혈액은 귀(이)동맥에서, 정맥혈액은 경정맥에서 채취하였다. 채혈한 혈액은 현장에서 즉시 휴대용 혈액가스분석기(EPOC$^{(R)}$ blood analysis, Woodly Equipment Company Ltd, Lancashire, UK)를 이용하여 pH, $pO_2$, $pCO_2$, $cHCO_3{^-}$, BE, $cSO_2$, $Na^+$, $Ca^{2+}$, $Cl^-$, anion gap potassium (AgapK), Hct, cHgb, glucose, lactate 및 creatinine을 측정하였다. 한우 송아지의 동맥과 정맥의 혈액가스, 전해질, 생화학 및 혈액학적 측정치는 기존에 발표된 정상 송아지의 측정치와 유사한 범위 내에 있었으며 glucose와 lactate의 평균 농도는 성우의 농도보다 높았다. 3주령 이내 송아지의 각 주령별 측정치 사이에는 통계적인 유의차가 나타나지 않았다(P > 0.05). 포도당 농도는 동맥과 정맥 혈액 사이에 가장 높은 상관관계를 보여주었으며(r = 0.927), creatinine (r = 0.925), lactate (r = 0.815), $Ca^{2+}$ (r = 0.806), Hct (r = 0.799), $Na^+$ (r = 0.790), cHgb (r = 0.786), base excess (r = 0.749), pH (r = 0.710), $HCO_3{^-}$ (r = 0.710), 및 $cTCO_2$ (0.663)도 높은 상관관계를 나타내었다. 휴대용 혈액가스분석기로 목장현장에서 혈액을 검사하는 것은 빠른 측정 결과를 얻을 수 있으며 소임상에서 유용하게 활용될 수 있을 것으로 생각된다.

• 한국임상수의학회지
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• 제37권4호
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• pp.231-234
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• 2020

An 11-year-old, 4.3 kg, mixed breed, intact female dog exhibiting vomiting and a reduced appetite was presented. On physical examination, palpation of the right kidney elicited discomfort. Blood analysis revealed leukocytosis with neutrophilia, and elevation of alkaline phosphatase and blood urea nitrogen. Escherichia coli was detected in urine culture, whereas urine analysis showed absence of glucose. Radiography and ultrasonography suggested that there was gas in the right renal parenchyma, and computed tomography was performed while the dog was awake to investigate the suspected gas in the right kidney. After unilateral ureteronephrectomy, a histological diagnosis of right emphysematous pyelonephritis (EPN) was made. EPN refers to a severe necrotizing infection of the renal parenchyma with gas accumulation in the tissue. It is rare in both human and veterinary medicine, and almost all EPN patients have uncontrolled diabetes mellitus. This case report describes the imaging-based diagnosis of a rare occurrence of unilateral EPN in a non-diabetic dog.

• Environmental Analysis Health and Toxicology
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• 제20권4호통권51호
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• pp.297-302
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• 2005

The purpose of this study was to provide the standard method for the analysis of organophosphorous pesticides such as chlorpyrifos, diazinon, malathion and parathion in blood. We performed method validation for these pesticides in blood according to EURACHEM (A focus For Analytical Chemistry in Europe) guide. For the analysis of the pesticides, we used solid-phase extraction ,column (Waters Oasis $HLB^{(R)}$. After the extraction, the supernatants were evaporated to dryness under the nitrogen stream. They were analyzed by gas chromatography/mass spectrometry (GC/MS) after reconstituting with ethanol. Terbufos was used as an internal standard. To validate this method, we performed verification procedures with the following parameters: selectivity, linearity of calibration, accuracy, precision, limit of detection and quantification. Validation data according to Eurachem guide were adequate for our purpose for the analysis of chlorpyrifos, diazinon, malathion and parathion in blood.

• Archives of Pharmacal Research
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• 제17권2호
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• pp.76-79
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• 1994

The concentration of styrne in blood of the occupationally syrene-exposed workers was checked by gas chromatographic headspace analysis. Mandelic acd in urine, that is a major metabolite of styrene, and hippuric acid wre also analyzed by high performance liquid chromatography. For the biological monitoring of styrene-exposed workers, the routine method of the quantitative determination of styrene nad its metabolites in the biolgical samples were studied.

• 대한의용생체공학회:학술대회논문집
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• 대한의용생체공학회 1992년도 추계학술대회
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• pp.103-104
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• 1992

Automatic transmission of data from the blood analyzer to the request site is one of the most important part in hospital computerization. We have developed a system to automatically transmit the data from the atrial blood gas analyzer. In this system, HOST computer, FILE server, LAN(Local Area Network), 3270 Emulator and Multi-port card were integrated. Also, 3 blood gas analyser(NOVA Inc., USA) were connected to a single multi-port card which is attached in a personal computer for data acquisition. When specimen is collected from sampling sites, it is transfered to the lab. After analysis, the result is transmitted to the personal computer via serial communication between machine and multi-port card using interrupt method. Then, the patient's information (Name, Sex, etc.) is obtained from the HOST computer througth the emulator. The combined data(patient information & lab data) is transmitted to the each request site via LAN automatically. From the collected data, patient's previous data could be reviewed, and it could be used for the various statistics and the flow chart for clinical research. Also, we found that this system reduces the personal labor.

• Tuberculosis and Respiratory Diseases
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• 제48권5호
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• pp.773-780
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• 2000

연구배경 : 흉수의 pH 측정은 흉막천자시 흉수의 원인 진단을 위해 시행되는 중요한 검사항목이며, 특히 부폐렴흉막염이 농흉화되는지를 확인할 수 있는 지표중 하나이다. 흉수의 pH 측정은 혈액가스분석기계를 이용하는 것이 표준이나, 상황에 따라서는 pH meter나 pH strip을 이용하기도 한다. 본 연구에서는 흉수중 삼출액을 대상으로 위의 세가지 방법으로 pH를 측정하고 측정치들을 비교하여 보았다. 방법 : 1999년 8월부터 2000년 3월까지 중앙대학교 부속 용산의료원에 입원하여 삼출성 흉수로 의심되어 흉막 천자를 시행한 34례 (결핵성 흉막염 16명, 부폐렴성 흉수 9명, 폐암 5명, 농흉 3명, 심부전 1명)의 흉수에서 혈액가스분석기계, pH meter, pH strip의 세 가지 방법으로 pH를 측정하였다. 혈액가스분석기계와 pH meter에 의한 측정은 천자후 $0^{circ}C$로 유지하면서 30분 내에 시행하였으며, pH strip 측정은 흉막천자 직후에 pH strip으로 pH를 측정하고, 상온 상태로 검사실에 내려서 보고되는 결과를 이용하였다. 결과 : 34례 흉수의 pH 값 (평균값$\pm$표준편차)은 혈액가스 분석법 7.34$\pm$0.12, pH meter 법 7.52$\pm$0.25, pH strip 법 중, 흉막천자후 즉시검사의 경우에는 7.37$\pm$0.16 였으며, 검사실에서 측정한 pH strip법은 6.93$\pm$0.20으로, 혈액가스분석법을 기준으로 한 평균치 분석에서 검사실에서 측정한 pH strip 법은 의미있는 차이를 보였다(p<0.05). 혈액가스분석법과 pH meter 방법, 그리고 혈액가스 분석법과 천자직후 pH strip법의 사이에는 의미있는 상관관계가 있었고, 혈액가스분석법과 pH strip 법 사이에는 의미있는 상관관계가 없었다. 결론 : 흉수천자 직후에 측정한 pH strip 법은 비교적 간단하고 정확하게 흉수의 pH를 측정할 수 있을 것으로 판단된다.

• Journal of Trauma and Injury
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• 제26권1호
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• pp.1-5
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• 2013

Purpose: The base deficit (BD) at admission in severely injured patients has been shown to predict the adequacy of resuscitation and outcome, but this relationship is not well established in the Korean experience. The purpose of this study was to define the association between arterial blood gas (ABG) values and the mortality for patients with severe blunt trauma at a developing trauma center in Korea. Methods: A retrospective review of 415 adult patients with severe blunt trauma was conducted using electronic medical records from Jan. 2010 to Dec. 2011. Results: A total of 256 patients had ABG drawn within 1 hour of arrival. Patients who expired displayed a higher lactate level (4.86 vs. 3.31, p<0.0001), a worse BD (-7.99 vs. -5.37, p=0.001), and a lower pH (7.31 vs. 7.34, p=0.011) at arrival compared with those who survived. A statistically significant association was also observed between BD and blood product usage (p=0.001). Conclusion: The base deficit at admission is a useful marker of mortality and outcome in severely injured patients with blunt trauma in Korea.