Diallel crosses among six silkworm varieties were used as the materials by the randomized block design. and combining ability tests were conducted to determine the relationships between parents and their F$_1$ hybrids. The six parents and their 30 F$_1$ crosses were evaluated for five quantitative characters in each female and male silkworms. Mean values of period(days) of larval stage in mid-parent were more than those of each F$_1$ hybrids. Highly significant differences were shown in the total cocoon weight and weight of cocoon layer of silkworms in F$_1$ hybrids of 111$\times$114, 111$\times$ yunil, 114$\times$yunil and those of reciprocal crosses. From the results, it was recognized that varieties A(111), D(114) and F(yunil) were useful varieties as the parents in breeding of silkworms for increasing the total cocoon weight and weight of cocoon layer, etc. Differences among crosses in apparent degree of heterosis existed for total cocoon weight of cocoon layer and cocoon layer ratio, etc. Mean square values of GCA (general combining ability) were more greater than those of SCA(specific combining ability) for period(days) of larval stage, period (days) of 5th instar and cocoon layer ratio of silkworms. The effects of GCA were differ from parents and characters and the effects of SCA were also differ from parents, characters and crosses.
To investigate the growth and nutrient uptake response of sorghum${\times}$sudangrass ($S{\times}S$) hybrid to different rate of livestock manure compost, a field experiment was conducted in the experimental grassland of Chonnam National University. Six treatments were laid out in a randomized block design with triplicates; control (no input), synthetic fertilizer (20 g N $m^{-2}$ and 20 g $P_2O_5\;m^{-2}$), compost 1 (3.4 g N $m^{-2}$ and 3.6 g $P_2O_5\;m^{-2}$), compost 2 (6.8 g N $m^{-2}$ and 7.2 g $P_2O_5\;m^{-2}$), compost 4 (13.4 g N $m^{-2}$ and 14.4 g $P_2O_5\;m^{-2}$), and compost 6 (20.2 g N $m^{-2}$ and 21.6 g $P_2O_5\;m^{-2}$). Ninety days after treatment, above-ground parts of the plants were harvested and measured for dry matter yield (DMY) and amounts of nutrients (N and P) uptake. Synthetic fertilizer application achieved the greatest DMY (2.4 kg $m^{-2}$) and nutrient uptake (38.3 g N $m^{-2}$ and 15.3 g $P_2O_5\;m^{-2}$). Increasing compost application rate tended to enhance DMY accumulation and nutrient uptake (P<0.01), but DMYs of compost 4 (1.9 kg $m^{-2}$) and 6 (1.8 kg $m^{-2}$) treatments were not different. Therefore, it was suggested that application compost alone may not achieve DMY of $S{\times}S$ hybrid compatible to synthetic fertilizer application. As nutrient uptake efficiency data showed that availability of compost P could be better than SF, it might be a strategy to apply compost as P source with supplementary N application such as liquid manure, SF or green manure if necessary considering availability of N input and the yield goals.
Pinus densiflora S. et Z. which has the widest distribution and highest stock at present in Korea and has used as materials for fuel, house construction, furniture and many other purposes for several hundred years, is considered as one of major species of economic importance, although there are a few epidemic insect injuries and silvicultural difficulties in regeneration. However, since disorderly cutting has been conducted for long time, the valuable local stands have disappeared. Therefore immediate attempts should be taken on the gene conservation and genetic studies including heritabilities and genetic gains of desirable characters. One hundred and twently five plus trees have so far been selected from the nationwide area and kept for the purpose of seed orchard establishment and other theoretical studies. In this study, the wind pollinated seeds of grafted stocks of 13 plus trees in clone bank located in Suweon and the seeds of 4 Japanese plus trees were collected and their progenies were used for heritability study. As indicated in figure 1, thirteen plus trees are from the middle part of Korea and two experimental plantations were laid out in ]972 (1-1 stock) by the randomized block design with five replications, consisting of 10 trees line plots of each family in each replication. Tree height, root collar diameter and branch diameter at 1cm apart from the main stem were measured. The results are summarized as follows 1. The rank of height growth of each plus tree progenies by age was greatly changed under age 3 and it was affected more in poor site than good site. 2. The heritabilities of height growth were estimated to be 7.2% at age 3, negative sign at age 4, 9.4% at age 5, 13.0% at age 6, 8.1% at age 7 and abrupt increase of 63.8% at age 8. The heritabilities were generally increased with increase of age. 3. The heritabilities of root collar diameter and branch diameter (average of the three biggest branches) was 3.2% and 11.8% at age 8 respectively. 4. The genetic gain was largest at age 8 and it was 46.6% when a selection intensity, 1/500 was taken. It, therefore, seems to be reasonable that selection should not be made under age 7.
This study was conducted to investigate the interaction of aflatoxin B$_1$($AFB_1$) and vitamin D$_3$($VD_3$) in broiler chicks. The 336 broiler chicks(Hubbard line) of equally mixed sex were allocated to triplicate 8(2$\times$4 factorial) treatment groups. The 0 or 1ppm of AFB$_1$and 0, 500, 1,000 or 1,500IU/kg of VD$_3$ were supplemented to the basal diet Fourteen broilers of equally mixed sex were allocated to each replica and 24 groups were arranged in a randomized block design After 3 weeks of feeding the metatarsus were collected from the right and left legs of 4 chicks (2 for each sex) per group. The bone ash and minerals were measured. 1. In respect to the fresh weight of metatarsus bone no significant difference was found between 0 and 1ppm $AFB_1$ treatments, however, decreasing trend was recognized when fed increasing level of $VD_3$(P<.01). 2. The ash content in non-fat dry metatarsus bone decreased when fed 1ppm $AFB_1$(P<.01). However, that increased according to the increasing amount of $VD_3$(P<.01). Although there was no interaction between $AFB_1$ and $VD_3$ it was shown that the 1500IU/kg of $VD_3$ was neccessary to cover the decrease in ash content of metatarsus. when fed 1ppm of $AFB_1$. 3. The Ca contents in metatarsus were not influenced by feeding $AFB_1$ but an increasing trend was verified by feeding increasing levels of $VD_3$(P<.05). 4. The P content decreased as $AFB_1$ was fed(P<.01), while no response was found when fed'different levels of $VD_3$ 5. The Cu content decreased when fed $AFB_1$(P<.05). 6. The Na, Mg, K, Zn, Fe and Mn contents were not affected by feeding $AFB_1$ and /or $VD_3$.
This study was conducted to determine effects of probiotics on performance, nutrient digestibility and nitrogen retention in Korean black goats fed two diets differing in forage to concentrate (F:C) ratio (30:70 and 70:30), for the establishment of their more efficient feeding management system. The probiotics employed in this trial were mixtures of different microbial species, which consisted of Lactobacillus casei, Bacillus subtilis, Saccharomyces cerevisiae, Aspergillus oryzae and Streptomyces griseus. Additional levels of probiotics to each F:C ratio (70:30 or 30:70) were 0 and 0.2%, respectively. Thus, twelve Korean black male goats were allotted to treatments in four groups of three goats per treatment and then they were housed in individual metabolism cages with a randomized complete block design for 21 days. Dry matter (DM) intakes were not affected by dietary F:C ratio and probiotics. Digestible DM amounts were significantly (p<0.05) decreased with increasing levels of dietary forage, but they were not affected by probiotics addition. Dry matter intakes per metabolic body weight and their ratio per body weight had a similar trend to DM intakes with no significant difference by F:C ratio and probiotics addition. The nutrient digestibility was significantly (p<0.05) increased with decreasing levels of forage in the diet, but it was not affected by probiotics addition. Urinary nitrogen loss was significantly (p<0.05) decreased with decreasing levels of dietary forage, but there was no significant difference between probiotics-supplemented and unsupplemented groups within the same F:C ratio. On the contrary, nitrogen retention was decreased with increasing levels of dietary forage, and probiotics supplementation to two diets differing in F:C ratio showed slightly increasing trends in the nitrogen retention. From the above results, probiotics supplementation to two diets differing in F:C ratio did not have the significant influence on feed intakes, nutrient digestibility and nitrogen retention. Consequently, these parameters of Korean black goats were dominated rather by F:C ratio than by dietary probiotics.
Kim, Young-Sun;Kim, Tack-Soo;Ham, Suon-Kyu;Ban, Su-Won;Lee, Chang-Eun
Asian Journal of Turfgrass Science
/
v.23
no.1
/
pp.111-122
/
2009
Compound fertilizer contained slow release nitrogen was used at golf course management because of promoting turfgrass growth and decreasing fertilizing labor. But, it was hardly published about turf grass growth by its effect and form like isobutylidene diurea(IBDU) and methylene urea(MU) in golf course management of Korea. This study was conducted to investigate the effect of compound fertilizer contained slow release nitrogen such as IBDU and MU on creeping bentgrasss and the change in nitrogen in the root zone after application of fertilizers at AM turfgrass research institute with soil analysis, chlorophyll content index, leaf area index and dry weight during 5 months from July to december in 2007 year. Fertilizer treatments were designed as following; non-fertilizer(NF), straight fertilizer(CF), treatment 1(MU), treatment 2(IBDU 1), and treatment 3(IBDU 2). The every treatments were arranged a randomized complete block design with three replications. Results obtained were summarized as follows: As relative to time after N fertilizers application, ammonium and nitrate in soil were increased, but T-N was decrease. $NO_3$-N and T-N content of slow release fertilizer(SRF), the treatment MU, IBDU 1 and IBDU 2, in soil was higher than that of CF, and the change of ratio of available N and T-N by elapsed time after fertilizing SRFs and CF was higher about 5% in root zone of SF to l5days, but in root zone of SRF from 30days about $5{\sim}10%$. Compared with NF, the turf color index was increased 6.5%, 6.7%, 5.9% and 5.5%, respectively, the chlorophyll content 33.2%, 38.4%, 35.1% and 37.1%, the dry weight 76.2%, 77.7%, 69.5 and 72.3% and shoot number 28%, 52%, 56% and 51%. The chlorophyll content index of MD, IBDU 1 and IBDU 2 was increased $2.6{\sim}5.2%$ than that of CF, shoot number $18{\sim}22%$ but turf color index and dry weight was analogous to that of CF. These results showed that the SRF application was supplied nitrogen for a long time in root zone by increasing an available nitrogen, and so bring turf growth such as chlorophyll content and shoot number in creeping bentgrass.
Kang, Hwan Ku;Kim, Ji-Hyuk;Hwangbo, Jong;Kim, Chan Ho
Korean Journal of Poultry Science
/
v.42
no.2
/
pp.181-189
/
2015
The objective of this study was to investigate the effect of dietary supplementation of vitamin C and Sea buckthorn on the performance, blood biochemistry and meat quality in old laying hens. A total 200 Hy-Line Brown laying hens (101 weeks old) were randomly allotted to 1 of 5 dietary treatments : (1) Basal : basal diet, (2) Vit. C : basal diet + 0.1% vitamin C, (3) SB 0.1 : basal diet + 0.1% Sea buckthorn, (4) SB 0.5 : basal diet + 0.5% Sea buckthorn, and (5) SB 1.0 : basal diet + 1.0% Sea buckthorn. Each treatment was replicated 4 times with 10 birds units were arranged according to randomized block design. Feeding trial lasted 4 weeks under 16L:8D lighting regimen. The diets were fed to hens on an ad libitum basis for 4 weeks. Result indicated that during feeding trial of the experiment, hen-day egg production and feed conversion ratio were not significantly influenced by treatments. However, feed intake was significantly (P<0.05) higher in Vit. C and SB treated groups than the basal during 1 wks and 3 wks. Egg weight was significantly (P<0.05) higher in basal and Vit. C than the SB 1.0 treatment. There were no differences in carcass yield during feeding trials. However, partial ratio (breast and neck) was significantly (P<0.05) higher in SB 0.5 than other treatment. There were no differences in the level of leukocytes and erythrocytes. There were no significant differences on proximate analysis (DM, crude protein, crude fat, and crude ash), meat color, water holding capacity, cooking loss, and fatty acids concentrations. In conclusion, dietary supplementation of vitamin C and Sea buckthorn to the diet of old laying hens might be a potential ingredient for increasing partial weight (breast) in old laying hens.
Kim, Chan Ho;Kang, Hwan Ku;Bang, Han Tae;Kim, Ji Hyuk;Hwangbo, Jong;Choi, Hee Cheol;Paik, In Kee;Moon, Hong Kil
Korean Journal of Poultry Science
/
v.41
no.4
/
pp.241-247
/
2014
The objective of this experimental was to investigate the effect of dietary Cu-sulfate and Cu-soy proteinate on productive performance and small intestinal microflora. A total 1,000 Hy-Line Brown laying hens (35 weeks old) were randomly allotted to 1 of 5 dietary treatments: Control, Cu-sulfate 50, 100 (50, 100 ppm Cu supplementation as Cu-sulfate) and Cu-SP 50, 100 (50, 100 ppm Cu supplementation as Cu-soy proteinate). Each treatment was replicated 4 times with fifty birds per replication, housed in 2 birds cages. Fifty birds units were arranged according to randomized block design. Feeding trial lasted 5 weeks under 16L : 8D lighting regimen. Hen day egg production was significantly (P<0.05) higher in Cu treated groups than control. Feed intake, broken and shell-less egg production was not significantly influenced by treatment. Eggshell color, eggyolk color, haugh unit, and eggshell thickness were not significantly influenced by treatment. However, eggshell strength was significantly (P<0.05) greater in Cu treated groups than control. Concentration of copper of liver was significantly (P<0.05) greater in Cu treated groups than control. Concentration of zinc and iron of liver were not influenced by treatments. Population of Cl. perfrigens and Lactobacilli in the small intestinal content were significantly (P<0.05) influenced by treatments. Population of Cl. perfrigens decreased and that of Lactobacilli increased in the Cu supplement groups. In conclusion, dietary Cu sulfate and Cu-soy proteinate similarly improves egg production, eggshell strength, and favors intestinal microbial population of laying hens.
Kim, Chan Ho;Kang, Hwan Ku;Bang, Han Tae;Kim, Ji Hyuk;Hwangbo, Jong;Choi, Hee Cheol;Paik, In Kee;Moon, Hong Kil
Korean Journal of Poultry Science
/
v.41
no.4
/
pp.323-329
/
2014
The objective of this experiment was to investigate the effect of dietary supplementation of copper-soy proteinate (Cu-SP) and herbal mixture (HBM) on growth performance, blood parameter, and immune response in laying hens. A total 800 Hy-Line Brown laying hens (60 weeks old) were randomly allotted to 1 of 4 dietary treatments : (1) Control : control diet, (2) Cu-SP : control diet + 100 mg/kg Cu-soy proteinate, (3) HBM : control diet + 0.15% herbal mixture, and (4) Cu-SP + HBM : control diet + 100 mg/kg Cu-soy proteinate + 0.15% herbal mixture. Each treatment was replicated 5 times with forty birds units were arranged according to randomized block design. Feeding trial lasted 5 weeks under 16L : 8D lighting regimen. The diet and water were available ad libitum. Result indicated that during feeding trial of the experiment, hen-day egg production was significantly (P<0.05) higher in Cu-SP and HBM treated groups than control. However, feed intake, feed conversion ratio, egg weight, broken and shell less egg production were not significantly influenced by treatments. Eggshell strength was significantly (P<0.05) higher in Cu-SP than control. Eggshell thickness, eggshell color, egg yolk color, Haugh unit were not significantly influenced by treatments. The level of WBC and stress index (heterophil : lymphocyte) were higher in supplemented groups than the control. The concentration of plasma IgG was higher in supplemented groups than the control. The result of this experiment showed that dietary copper-soy proteinate or herbal mixture tended to improve egg production and affect positively on immune response of laying hens.
The experiment was conducted to investigate the effects of feeding herbaceous peat on growth performance and meat quality of Holstein beef cattle. Total of 20 Holstein beef cattle (18~20 month of age, $657{\pm}31kg$ body weight) were conventionally and separately fed a concentrate diet and rice straw for 134 days. The dietary treatments were randomly assigned by complete block design into four treatments, each of which were five heads in early fattening stage. The treatments in this study were the control group fed basal diet, feeding herbaceous peat group (5%/diet, T1), feeding coated vitamin C group (20g/head, T2) and feeding mixture of herbaceous peat and coated vitamin C group (5%/diet+20g/head, T3). The initial body weights between the groups of control, T1, T2 and T3 were similar showing with $689{\pm}31$, $661{\pm}24$, $659{\pm}32$ and $622{\pm}19kg$. The daily body weight gain was higher in T3 by 8.3% than that in the control (p<0.05). Glucose concentration in control group was the highest among treatments (p<0.05), but there was no significant differences between treatments on AST (aspartate aminotransferase), ALT (alanine aminotransferase), BUN and total protein concentrations of blood. The fat content of sirloin in the T2 was significantly higher than control and T1 group (p<0.05). Meat color (CIE) values in T2 was the highest among treatments (p<0.05), and other treatments also increased those values. In overall, the feeding herbaceous peat and vitamin C to the Holstein beef cattle was considered to have positive effects on the growth performance of Holstein beef cattle. In addition, the effects on the performances of animals were more improved when fed herbaceous peat and vitamin C concurrently.
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