• Title/Summary/Keyword: blast analysis

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16S rDNA-PCR and RFLP Analysis for rapid identification of Spoilage Bacteria from low Salt Cucumber Brine (저염 발효오이로부터 16S rDNA-PCR과 RFLP분석을 통한 부패균의 신속한 확인)

  • 김재호;장혜영
    • KSBB Journal
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    • v.19 no.1
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    • pp.72-77
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    • 2004
  • The aim of this study was to isolate and identify the spoilage bacteria in the low salt cucumber brine. The PCR amplicons comprising a portion of the 16S rRNA gene of the isolated colonies were directly sequenced and the untrimmed whole sequencing results of the unknown strains were aligned with the type strains using BLAST of NCBI. Then Sequence Aligner and Sequence Match of RDP confirmed the outcome. The identified isolates were eight species and belong to three genuses: Clostridium, Lactobacillus, and Bacillus. The RFLP pattern of the 16S rRNA gene of isolates verified the identified species. From now on the complex spoiling process of law salt fermented cucumber could be analyzed using the isolated species individually or with certain combinations.

A clinical study of desensitizing agent on hypersensitive teeth (지각과민 치아에 대한 처치약재의 임상적 연구)

  • Lee, Seong-Min;Lee, Chan-Young;Lee, Seung-Jong;Park, Dong-Soo
    • Restorative Dentistry and Endodontics
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    • v.14 no.1
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    • pp.135-148
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    • 1989
  • The purpose of this study was to evaluate the periodic effect of desensitizing drug such as potassium oxalate(D.D.S. # I&II), strontium chloride (ZAROSEN)$^{(R)}$, and placebo group. The 193 teeth of 93 patients who had been complained dental hypersensitivity, and were divided into three groups by application agent and desensitizing treatment was completed. The interval of observation and treatment period were immediately, 1 week, 2 week, 3 week, 4 week, before and after treatment. The data was statistically analized and the results were as followed. 1. Group I showed best desensitizing effect to the stimuli, followed by Group II, Group III. 2. There was a significant difference (p < 0.005) in desensitizing effect among the Group I, Group III and Group II, Group III but there was no significant difference (p < 0.005) in Group I, Group II. 3. The cold stimuli was most effective in desensitization and there was a significant difference (p < 0.005) in cold, air-blast, but there was no significant difference (p < 0.005) in other stimuli. 4. There was no significant difference (p < 0.005) in effect of the desensitization of the cause of exposed dentine. 5. Anterior teeth was more effective than posterior teeth in desensitization and there was a significant difference (p < 0.005) between anterior teeth and posterior teeth. 6. In analysis of stimuli on the potassium oxalate, there was a significant difference (p < 0.005) in cold, air-blast but there was no significant difference (p < 0.005) in other stimuli.

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Influence of Delay Time and Priming Location on the Blast-Induced Ground Vibration (발파공 사이의 지연시차와 기폭위치가 지반진동에 미치는 영향)

  • Kang, Choo Won;Ryu, Bok Hyun;Choi, Tae Hong
    • Tunnel and Underground Space
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    • v.24 no.1
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    • pp.97-109
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    • 2014
  • In order to identify the characteristics of the propagation depending on delay time (20, 25 ms) and priming location (top priming, middle priming, bottom priming), test blasts were carried out a total of 4 times using different spacing, burden, drilling length, charge per delay and was derived the formula to predict blast vibration. This study investigated the characteristics of vibration by analysis of the nomogram and prediction of Peak Particle Velocity (PPV) from delay time and priming location by the formula to predict ground vibration. And it analyzed the trends of vibration increase by standards charge 0.5, 1.6, 5, 15 kg. Standards charge is "Blasting design and construction guidelines to road construction" by the Ministry of Land, Infrastructure and Transport. Depending on the charge in favor of vibration control method is proposed. Thus, when the design was to be used as a variable.

On the vibration influence to the running power plant facilities when the foundation excavated of the cautious blasting works. (S 화력발전소 3, 4호기 증설에 따르는 정밀발파작업으로 인한 인접가동발전기 및 구조물에 미치는 진동영향조사)

  • Huh Ginn
    • Explosives and Blasting
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    • v.9 no.4
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    • pp.3-12
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    • 1991
  • The cautious blasting works had been used with emulsion explosion electric M /S delay caps. Drill depth was from 3m to 6m with Crawler Drill 70mm on the calcalious sand stone (soft-moderate-semi hard Rock) . The total numbers of feet blast were 88. Scale distance were induces 15.52-60.32. It was applied to Propagation Law in blasting vibration as follows .Propagtion Law in Blasting Vibration V=k(D/W/sup b/)/sup n/ where V : Peak partical velocity(cm/sec) D : Distance between explosion and recording sites(m) W ; Maximum Charge per delay -period of eight milliseconds or more(Kg) K : Ground transmission constant, empirically determind on the Rocks, Explosive and drilling pattern ets. b : Charge exponents n : Reduced exponents Where the quantity D/W/sup b/ is known as the Scale distance. Above equation is worked by the U.S Bureau of Mines to determine peak particle velocity. The propagation Law can be catagrorized in three groups. Cabic root Scaling charge per delay Square root Scaling of charge per delay Site-specific Scaling of charge delay Charge and reduction exponents carried out by multiple regressional analysis. It's divided into under loom and over loom distance because the frequency is varified by the distance from blast site. Empirical equation of cautious blasting vibration is as follows. Over 30m--under 100m----V=41(D/ W)/sup -1.41/-----A Over l00m---------V=121(D/ W)/sup -1.56/-----B K value on the above equation has to be more specified for furthur understand about the effect of explosives. Rock strength, And Drilling pattern on the vibration levels, it is necessary to carry out more tests.

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Fabrication of the Cement for the Solidification of the Toxic Waste using Waste Concrete Powder (폐콘크리트 미분말을 이용한 유해 폐기물 고화용 시멘트의 제조)

  • Kim, In-Seob;Won, Jong-Han;Choi, Kwang-Hui;Choi, Sang-Hul;Lee, Jong-Gyu;Sohn, Jin-Gun;Shim, Kwang-Bo
    • Journal of the Korean Ceramic Society
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    • v.39 no.12
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    • pp.1133-1137
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    • 2002
  • The cement for solidification of the toxic waste was fabricated using a mixture of the waste concrete powder and blast furnace slag in the ratio of 1:1 and its hydrate morphology and compressive strength of the sample were evaluated in order to apply to the solidification of the COREX sludge. The X-ray diffraction analysis of the sample which prepared by the addition of 10% Portland cement and hemihydrate showed the presence of $Ca(OH)_2$, ettringite, gel-phase and C-S-H hydrate. Compressive strength of the sample exhibited enough high to use as a solidification cement. The strength of the sample was over 140 kgf/$m^2$ in 7 days in case of solidification of the COREX sludge and the sample possess sufficient morphology for the solidification and stabilization of the waste sludge.

An Experimental Study on Compression Strength and Carbonation Resistance for Ternary High-Performance Concrete with fly-sah, granulated blast furnace (플라이애쉬와 고로슬래그를 사용한 3성분계 고성능 콘크리트의 강도 및 촉진 중성화에 대한 실험적 고찰)

  • Kwon, Young-Rak;Kim, Hong-Sam;Lee, Chang-Young;Cheng, Hai-Moon;Ahn, Tae-Song
    • Proceedings of the Korea Concrete Institute Conference
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    • 2008.04a
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    • pp.445-448
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    • 2008
  • It is essential that concrete component is made up with aggregate, cement and water. But today, Public concern is increasing of a variety structure and ocean environmental, resource recycle. Also, According to heat of hydration rising, Concrete is make a causative of concrete-crack. Concrete-crack cause a falling-off in quality of concrete. consequently, High-performance concrete is evaluated by concrete material properties and carbonation resistance with different admixture(fixing fly-ash 20%), granulated blast furnace slag replacement ratio (30%, 45%) different W/B (26%, 30%, 34%) and XRD(X-ray Diffraction) analysis.

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Engineering Characteristics Analysis of High Strength Concrete Followed in replacement ratio increase in Blast Furnace Slag (고로슬래그 미분말의 치환율 증가에 따른 고강도 콘크리트의 공학적 특성 분석)

  • Han, Cheon-Goo;Kim, Seoung Hwan;Son, Ho-Jung
    • Journal of the Korean Recycled Construction Resources Institute
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    • v.4 no.3
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    • pp.62-68
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    • 2009
  • This research examined engineering properties of high performance concrete, when substitution rate of BS increases. A summary of the test result is as follows. The fluidity of unset concrete increases as the substitution rate of BS increases. The amount of air is reduced more or less, but it seems that enough amount of air can be secured by using more air-entraining agent. Setting time is dramatically delayed as the substitution rate of BS increases. The compressive strength of hardening concrete was weaker than OPC before 28 days passes, due to latent hydraulic property of BS. However, after 28 days, it shows same or better property, which is exceptional for the practical uses of hyper strength concrete. Changes in drying shrinkage rate is quite much, because when hydration happens, the amount of free water in concrete increased as W/B gets larger. The amount of drying shrinkage increases as BS substitution rate increases, but every composition shows less than $-500{\times}10^{-6}$, which is relatively fine.

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Pi5 and Pii Paired NLRs Are Functionally Exchangeable and Confer Similar Disease Resistance Specificity

  • Vo, Kieu Thi Xuan;Lee, Sang-Kyu;Halane, Morgan K.;Song, Min-Young;Hoang, Trung Viet;Kim, Chi-Yeol;Park, Sook-Young;Jeon, Junhyun;Kim, Sun Tae;Sohn, Kee Hoon;Jeon, Jong-Seong
    • Molecules and Cells
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    • v.42 no.9
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    • pp.637-645
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    • 2019
  • Effector-triggered immunity (ETI) is an effective layer of plant defense initiated upon recognition of avirulence (Avr) effectors from pathogens by cognate plant disease resistance (R) proteins. In rice, a large number of R genes have been characterized from various cultivars and have greatly contributed to breeding programs to improve resistance against the rice blast pathogen Magnaporthe oryzae. The extreme diversity of R gene repertoires is thought to be a result of co-evolutionary history between rice and its pathogens including M. oryzae. Here we show that Pii is an allele of Pi5 by DNA sequence characterization and complementation analysis. Pii-1 and Pii-2 cDNAs were cloned by reverse transcription polymerase chain reaction from the Pii-carrying cultivar Fujisaka5. The complementation test in susceptible rice cultivar Dongjin demonstrated that the rice blast resistance mediated by Pii, similar to Pi5, requires the presence of two nucleotide-binding leucine-rich repeat genes, Pii-1 and Pii-2. Consistent with our hypothesis that Pi5 and Pii are functionally indistinguishable, the replacement of Pii-1 by Pi5-1 and Pii-2 by Pi5-2, respectively, does not change the level of disease resistance to M. oryzae carrying AVR-Pii. Surprisingly, Exo70F3, required for Pii-mediated resistance, is dispensable for Pi5-mediated resistance. Based on our results, despite similarities observed between Pi5 and Pii, we hypothesize that Pi5 and Pii pairs require partially distinct mechanisms to function.

A Calibration Method of the CSC Model for Considering Material Properties of Ultra-high Performance Concrete (초고성능 강섬유 보강 콘크리트 물성 반영을 위한 소성 기반 콘크리트 CSC 모델 보정기법)

  • Gang-Kyu, Park;MinJoo, Lee;Sung-Wook, Kim;Hyun-Seop, Shin;Jae Heum, Moon
    • Journal of the Korean Recycled Construction Resources Institute
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    • v.10 no.4
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    • pp.402-410
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    • 2022
  • The present study introduces a calibration method of the CSC model implemented in the LS-DYNA program for considering the material properties of ultra-high performance concrete(UHPC). Based on previous experimental studies, various parameters, which constitute three shear failure surfaces, pressure-volumetric strain curve, fracture energy, dynamic increase factor(DIF), and so on, are modified. Then, the proposed calibration method is verified by comparing the numerical result with the experimental data through the single element analysis. In addition, based on the established finite element models, the applicability of the calibrated CSC model is examined for UHPC structures subjected to impact and blast loadings.

Strength and toughness prediction of slurry infiltrated fibrous concrete using multilinear regression

  • Shelorkar, Ajay P.;Jadhao, Pradip D.
    • Advances in concrete construction
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    • v.13 no.2
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    • pp.123-132
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    • 2022
  • This paper aims to adapt Multilinear regression (MLR) to predict the strength and toughness of SIFCON containing various pozzolanic materials. Slurry Infiltrated Fibrous Concrete (SIFCON) is one of the most common terms used in concrete manufacturing, known for its benefits such as high ductility, toughness and high ultimate strength. Assessment of compressive strength (CS.), flexural strength (F.S.), splitting tensile strength (STS), dynamic elasticity modulus (DME) and impact energy (I.E.) using the experimental approach is too costly. It is time-consuming, and a slight error can lead to a repeat of the test and, to solve this, alternative methods are used to predict the strength and toughness properties of SIFCON. In the present study, the experimentally investigated SIFCON data about various mix proportions are used to predict the strength and toughness properties using regression analysis-multilinear regression (MLR) models. The input parameters used in regression models are cement, fibre, fly ash, Metakaolin, fine aggregate, blast furnace slag, bottom ash, water-cement ratio, and the strength and toughness properties of SIFCON at 28 days is the output parameter. The models are developed and validated using data obtained from the experimental investigation. The investigations were done on 36 SIFCON mixes, and specimens were cast and tested after 28 days of curing. The MLR model yields correlation between predicted and actual values of the compressive strength (C.S.), flexural strength, splitting tensile strength, dynamic modulus of elasticity and impact energy. R-squared values for the relationship between observed and predicted compressive strength are 0.9548, flexural strength 0.9058, split tensile strength 0.9047, dynamic modulus of elasticity 0.8611 for impact energy 0.8366. This examination shows that the MLR model can predict the strength and toughness properties of SIFCON.