• Title/Summary/Keyword: black water

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A New Lichen-Forming Fungus, Aspicilia humida, from a Forested Wetland in South Korea, with a Taxonomic Key for Aspicilioid Species of Korea

  • Lee, Beeyoung Gun;Shin, Hyun Tak;Hur, Jae-Seoun
    • Mycobiology
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    • v.50 no.1
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    • pp.20-29
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    • 2022
  • Aspicilia humida Lee is described as a new lichen-forming fungus from a wetland forest, South Korea. The new species is distinguishable from Aspicilia aquatica (Fr.) Körb., the most similar species, by the absence of prothallus, black disk without green color in water, olivebrown epihymenium, shorter hymenium, hymenium I + yellowish blue-green, wider paraphysial tips without a vivid pigment, smaller asci, smaller ascospores, and the presence of stictic acid. Molecular analyses employing internal transcribed spacer (ITS) and mitochondrial small subunit (mtSSU) sequences strongly support A. humida as a distinct species in the A. cinerea group. A surrogate key is provided to assist in the identification of all 28 aspicilioid species of Korea.

Effect of PE Film Mulching and Irrigation Method on the Growth, Yield and Antioxidant Activity for Potatoes Grown in Winter Season at Saemangeum Reclaimed Land (새만금 간척지에서 감자 겨울재배시 비닐멀칭 및 관수방법이 생육, 수량 및 괴경의 항산화 활성에 미치는 영향)

  • Choi, Weon-Young;Cho, Kwang-Min;Kim, Sun;Jeong, Jae-Hyeok;Lee, Su-Hwan;Lee, Kyeong-Bo;Lee, Geon-Hwi;Park, Ki-Hun
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.60 no.1
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    • pp.63-69
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    • 2015
  • This study was conducted to measure the effect of PE film mulching and irrigation methods on the growth, yield and antioxidant activity of potatoes tubers, in order to examine the possibility of cultivating potatoes in winter season in vinyl greenhouse on the reclaimed tidal land with weak ground inside the sea wall currently completed. The test was conducted on the sandy loam soil (Munpo series), and its salt concentration was 0.42% at the time of planting. The emergence speed per kind of PE film mulching was in the order of black > coloration > transparent > green color, with the black color showing the fastest speed. The temperature change during a day per kind of PE film mulching was in the order of transparent > coloration > green > black color. As for the salt concentration in the soil for each different way of water management, the salt concentration in the treatment of drip irrigation with 1 week interval was lower than that drip irrigation with 2 weeks interval. As for the growth of above-aerial part, plant length was higher, number of tiller and leaves were more and dry weight of above-aerial part was larger in the treatment of drip irrigaton with 1 week interval than drip irrigation with 2 weeks interval. As for the yield of potatoes depending on each way of water management, the yield in the treatment of drip irrigation with 1 week interval was more than drip irrigation with 2 weeks interval. The yield for each different kind of PE film mulching in the weekly drip-irrigation management section was in the order of transparent ${\geq}$ black ${\geq}$ coloration ${\geq}$ green color. In both of total phenol content and DPPH free radical activity experiments, the content and activity were higher with pear color vinyl treatment.

Taxonomical Classification of Namweon Series, Black Volcanic Ash Soils (흑색 화산회토인 남원통의 분류)

  • Song, Kwan-Cheol;Hyun, Byung-Geun;Sonn, Yeon-Kyu;Lim, Han-Cheol;Lee, Shin-Chan
    • Korean Journal of Soil Science and Fertilizer
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    • v.42 no.5
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    • pp.385-392
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    • 2009
  • This study was conducted to reclassify Namweon series, black volcanic ash soils, in Jeju Island based on the second edition of Soil Taxonomy : A Basic System of Soil Classification for Making and Interpreting Soil Surveys. Morphological properties of typifying pedon of Namweon series were investigated and physicochemical properties were analyzed according to Soil Survey Laboratory Methods Manual. The typifying pedon of Namweon series has black (10YR 2/1) silt loam Ap horizon (0~11 cm) and black (10YR 2/1) silt loam BA horizon (11~72 cm). Bw horizon (72~100 cm) is very dark brown (10YR 2/2) silt loam. That occurs on lava plain derived from volcanic ash materials. The typifying pedon contains 5.2~6.4% oxalate extractable (Al + 1/2 Fe), over 85% phosphate retention, and lower bulk density than $0.90Mg\;m^{-3}$. Ap, BA, and Bw horizons of the pedon have andic soil properties. That can be classified as Andisol. The typifying pedon has an udic soil moisture regime and has a 1,500 kPa water retention of 15% or more on air-dried samples throughout all horizons, and can be classified as Udand. Ap and BA horizons (0~72 cm) have a color value, moist, and chroma of 2 or less, melanic index of 1.70 or less, and 6% or more organic carbon. That meets the requirements of melanic epipedon. That keys out as Melanudand. That has more than 6.0% organic carbon and the colors of mollic epipedon throughout a layer 50 cm or more thick within 60 cm of the mineral soil surface.. Thus, that keys out as Pachic Melanudand. The pedon has a fine-earth fraction that has a water content at 1,500 kPa tension of 12% or more on air-dried samples and has less than 35% (by volume) rock fragments. Thus, the substitute for particle-size class is medial. That has a sum of 8 times the Si (percnt by weight extracted by acid oxalate) plus 2 times the Fe (percnt by weight extracted by acid oxalate) of 5 or more, and 2 times the Fe is more than 8 times the Si. Thus, the mineralogy class is ferrihydritic. Namweon series can be classified as medial, ferrihydritic, thermic family of Pachic Melanudands, not as ashy, thermic family of Typic Melanudands.

Transplantation of Koelreuteria paniculata by Sea Current (모감주나무의 해류에 의한 전파)

  • 이영노
    • Journal of Plant Biology
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    • v.1 no.1
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    • pp.11-20
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    • 1958
  • I. Assumption: Mo-kam-ju, Koelreuteria paniculata is a woody plant mainly cistributed over Northern China. Its sporadic growth along the beaches of Korea and Japan is assumed to have been sowed by the seeds transported adrift on the current across the ocean. II. Use and Growth of the Plant: In China, this plant has been cultivated from early times mainly at temples, it seeds being used as rosaries, its flowers for yellow dyes and medicine for the eyes, and its leaves for black dyes. In Korea and Japan, these plants have been cultivated at temples and used as the material for rosaries. No natural growth of these plants was reported until 1919. III. Discoveries: In this domestic area, Dr. Chung Tae Hyun discovered the plant on the beach between Cho-Do and Chang-san-kot, Hwang-Hae Prov. in 1920. The reporter discovered them on the beach at Buk-Ni, Duk-jok-Do in 1948, at An-Hung in 1956 and on the beach at An-min-Do in 1957. In the Japan area, it was discovered for the first time twenty years ago, mainly along the coast line of the Japan Sea and some along the eastern coast line, at Subo, Yamaguchi Prefecture facing the Pacific Ocean. IV. Study and Experiment: A. Seed The seed coat is thick, non-permeable and floatable. A number of seeds were immersed in artificial sea water and fresh water separately. The seeds remained there for a hundred and forty-five (145) days from April 6, 1957 to August 29, 57. Thirty one seeds out of the fifty immersed in salt water and twenty seeds out of the fifty immersed in fresh water remained on the surface of the water, proving them to be non-permeable to both sea and fresh water. Of course, these had retained their germinating capabilities. Five (5) seeds out of twenty from the fresh water and six (6) out of thirty-one from the sea water were successfully germinated after a hole had been drilled in the seed coat and they had been planted. Thus their floating capability, non-permeability and germinating capability after a possible 145 day trip on the flowing currents has been proved satisfactory according to the assumption made above. B. Current As shown in the Data 2 and 3, the sea current initiated in the Pohai Sea flows westward down along the coast line of Korea reaching the southern part during the autumn and winter seasons. This fact also is in favor of the reporter's assumption. V. Discussion and Conclusion: The reporter concludes that, as discussed above, Koelreuteria paniculata which originates in Northern China is transported adrift on the flowing sea current to our western coast line, and also the coast lines of Shantung and Kuangtung of China and it is germinated on the sandy beaches forming new plants. Thus, the seeds drifted down on the southern beach of Korea and have been transported to the Japanese coast, adrift on the Tae-Ma current. Upon fruition, the seeds of the plants which settle on the coasts of western Korea and Japan will migrate to new places. It can be, however, assumed that while the thickness and nonpermeability of the coat enables the long travel in the water, this also can compose a difficulty in germination, consequently in developing a new distribution of this species.

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Effects of Aged Black Garlic Extracts on the Tight Junction Permeability and Cell Invasion in Human Gastric Cancer Cells (흑마늘 추출물이 인체위암세포의 tight junction 투과성 조절과 세포 침윤성 억제에 미치는 영향)

  • Shin, Dong-Yeok;Yoon, Moo-Kyoung;Choi, Young-Whan;Gweon, Oh-Cheon;Kim, Jung-In;Choi, Tae-Hyun;Choi, Yung-Hyun
    • Journal of Life Science
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    • v.20 no.4
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    • pp.528-534
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    • 2010
  • Garlic (Allium sativum) has been well-known as a folk remedy for a variety of ailments since ancient times, and it is well documented that enhanced garlic consumption leads to a decrease in incidences of cancer. Tight junctions (TJs) are critical structures for the maintenance of cellular polarity, acting as paracellular permeability barriers and playing an essential role in regulating the diffusion of fluid, electrolytes and macromolecules through the paracellular pathway. Matrix metalloproteinases (MMPs) have been implicated as possible mediators of invasiveness and metastasis in some cancers. In this study, we investigated the potential effects of water extract of aged black garlic (ABG) on the correlation between tightening of TJs and anti-invasive activity in human gastric carcinoma AGS cells. The inhibitory effects of ABG on cell motility and invasiveness were found to be associated with increased tightness of TJs, which was demonstrated by an increase in transepithelial electrical resistance. Additionally, the activities of MMP-2 and -9 in AGS cells were inhibited by treatment with ABG, and this was also correlated with a decrease in the expression of their mRNA and proteins. Furthermore, RT-PCR and immunoblotting results indicated that ABG repressed the levels of the claudin proteins, major components of TJs that play a key role in the control and selectivity of paracellular transport. In conclusion, these results suggest that ABG treatment may inhibit tumor metastasis and invasion, and therefore may act as a dietary source to decrease the risk of developing cancer.

Physicochemical Properties and Dietary Effect of Glycoprotein from Sea Cucumber(Stichopus japonicus) (해삼 당단백질의 물리화학적 특성과 식이효과)

  • 류홍수;문정혜;유병진;문수경
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.25 no.2
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    • pp.240-248
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    • 1996
  • To clarify the potentiality of sea cucumbers as dietary food, the effects of those glycoprotein on dietary proteins and physicochemical properties of those proteins were studied. Crude glycoprotein was efficiently extracted using 20mM sodium phosphate beffer(pH 7.0) and by salting out with 80% ammoniym sulfate saturation. The fractions obtained through the DEAE-cellulose ion exchange chromatography was identified as glycoprotein by Schiff's reagent and SDS polyacrylanide gel electro-phoresis. The yields of each glycoprotein from the three kinds of sea cucumbers were 0.814(red), 0.184(blue) and 0.232(black) and the molecular weights of the glycoproteins subunits were ranged from 20,000 dalton(blue and black) to 29,000 dalton(red), respectively. The electrophoretic patterns of the glycoprotein isolates were similar to each other and any significant difference in amino acid pattern was observed. Predominant arnino scids were Asx(aspartic acid and asparagine) and Glx(glutamic acid and glutamine) ; in contrast, histidine and methionine were below 2% as compared to total amino acids. water holding capacities of the glycoprotein isolates from red, blue and black cucumbers were equally 100% and emulsion activities ranged from 53% to 64%. In addition the emulsion stabilities were 7.04, 1.37 and 2.44, respectively. In vitro digestibility of some proteins(casein, SPI and squid) was decreased as increasing the level of the freeze dried sea cucumber powder and glycoprotein isolates. But squid protein was not affected.

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Physicochemical Properties of Black Rice Flours (BRFs) Affected by Milling Conditions (제분조건에 따른 흑미쌀가루 품질특성)

  • Choi, Bong-Kyu;Kum, Jun-Seok;Lee, Hyun-Yu;Park, Jong-Dae
    • Korean Journal of Food Science and Technology
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    • v.38 no.6
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    • pp.751-755
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    • 2006
  • Physicochemical properties of black rice flours (BRFs) produced under different milling conditions were investigated to explore their applications for various processed rice products. Moisture contents of BRFs after roll milling and subsequent hot-air drying or microwave drying were 10.3% and 14.4%, respectively. The BRFs with jet milling and subsequent hot-air drying or microwave drying showed moisture contents of 7.9% and 7.5%. Mean particle sizes of the BRFs obtained from roll milling, jet milling, and following air-classification were 150, 41.5, and 9.4 ${\mu}m$, respectively. Hunter L value and water soluble index (WSI) of BRFs increased with decreasing particle size, while Hunter a value decreased. Total polyphenol contents of 91.4-115.8 mg/100 g and total amino acids of 75.0-96.4 mg/100 g in three types of BRFs were higher as compared to those (6.3 mg/100 g and 30.5 mg/100 g) of polished rice flour (PRF). Physicochemical properties such as moisture content, color parameter, and WSI have close relationship with the particle size of BRFs prepared under different milling conditions.

Production of Cloned Jeju Black Cattle (Korean Cattle) from SCNT Embryo using Vitrification, One-Step Dilution and Direct Transfer Technique (초자화 동결과 1-단계 융해된 체세포 핵이식란의 직접 이식 기술로 제주흑우 복제소 생산)

  • Kim, Eun-Young;Park, Min-Jee;Kim, Jae-Youn;Park, Hyo-Young;Noh, Eun-Ji;Noh, Eun-Hyung;Song, Dong-Hwan;Oh, Chang-Eon;Kim, Young-Hoon;Mun, Seong-Ho;Lee, Dong-Sun;Ko, Moon-Suck;Riu, Key-Zung;Park, Se-Pill
    • Reproductive and Developmental Biology
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    • v.35 no.1
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    • pp.77-83
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    • 2011
  • One-step dilution and direct transfer would be a practical technique for the field application of frozen embryo. This study was to examine whether Jeju Black Cattle (JBC, Korean Cattle) can be successfully cloned from vitrified and one-tep diluted somatic cell nuclear transfer (SCNT) blastocyst after direct transfer. For vitrification, JBC-SCNT blastocysts were serially exposed in glycerol (G) and ethylene glycol (EG) mixtures [10%, (v/v) G for 5 min., 10% G plus 20% EG (v/v) for 5 min., and 25% G plus 25% EG (v/v) for 30 sec.] which is diluted in 10% FBS added D-PBS. And then SCNT blastocysts were loaded in 0.25 ml mini straw, placed in cold nitrogen vapor for 3 min. and then plunged into $LN_2$. One-step dilution in straw was done in $25^{\circ}C$ water for 1 min, by placing vertically in the state of plugged-end up and down for 0.5 min, respectively. When in vitro developmental capacity of vitrified SCNT blastocyst was examined at 48 h after one-step dilution, hatched rate (56.4%) was slightly lower than that of control group (62.5%). In field trial, when the vitrified-thawed SCNT blastocysts were transferred into uterus of synchronized 5 recipients, a cloned female JBC was delivered by natural birth on day 299 and healthy at present. In addition, when the short tandem repeat marker analysis of the cloned JBC was evaluated, microsatellite loci of 11 numbers was perfectly matched genotype with donor cell (BK94-14). This study suggested that our developed vitrification and one-step dilution technique can be applied effectively on field trial for cloned animal production, which is even no longer in existence.

Preparation and Characterization of Wet Noodle Containing Germinated Small Black Bean Flour (발아약콩가루를 첨가한 생면의 제조 및 특성연구)

  • Han, Sung-Mi;Han, Jung-Ah
    • Korean Journal of Food Science and Technology
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    • v.43 no.5
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    • pp.597-602
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    • 2011
  • Small black beans (SBB) were germinated up to 72 h at 23$^{\circ}C$, and 48 h germinated SBBs having the highest isoflavone content were chosen for noodle preparation. Some of the germinated SBB were treated by 20 min boiling (B) but some were not boiled (NB). For noodles, the germinated SBB flour replaced 15% or 20% of wheat flour, so five kinds of noodles were prepared and tested: control, 15B, 15NB, 20B, and 20NB. Higher amount of bean flour resulted in significant decrease of $L^*$ and increase of $b^*$ values in noodle. After 20 min cooking, the noodles containing germinated SBB flour showed less soluble sugar content in cooked water than control, and the lowest value was observed in 15B. For textural properties, the addition of SBB flour lowered hardness and adhesiveness of noodle, but increased chewiness than control. In sensory tests, the most acceptable sample was 15B because of developed texture and better appearance which seem to be good for health.

Influence of red mud additive on lightening of artificial aggregates containing coal bottom ash (석탄바닥재가 포함된 인공골재의 경량화에 미치는 적니 영향)

  • Kang, Min-A;Kang, Seung-Gu
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.21 no.1
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    • pp.41-46
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    • 2011
  • The artificial aggregates (AAs) composing of 2 wastes, coal bottom ash and dredged soil (7 : 3, weight ratio) were fabricated as a function of red mud contents,0~30 wt% using direct sintering method at $1050{\sim}1250^{\circ}C$ for 10 min, and those physical properties were evaluated. Especially, in order to analyze the red mud addition effect on the bloating phenomenon of AAs manufactured, the specific gravity and water absorption were measured and studied linked with the microstructural observation results. The lightening of AAs was enhanced due to increased bloating with increasing temperature and red mud contents. The AAS sintered at $1050{\sim}1150^{\circ}C$ showed well-developed black-coring structure, but for the specimens containing red mud sintered over $1200^{\circ}C$ generated excessive liquid and gas caused by reduction of $Fe_2O_3$, thus the black-coring part was gradually burst open out of shell of AAs. Particularly, all specimens containing 30 wt% red mud was burst up when sintered over $1100^{\circ}C$. The AAs containing no red mud sintered at $1200^{\circ}C$ had a specific gravity of about 1.2 and those containing 20 wt% had below 1.0 which are characters of lightweight aggregate.