• Archives of Pharmacal Research
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• v.28 no.12
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• pp.1350-1357
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• 2005

Nuclear factor kappa B (NF-$\kappa$B) regulates the expression of multiple cytokines, chemokines, and cell adhesion molecules that are involved in the pathogenesis of asthma. We investigated the anti-asthmatic effects and the mechanism of action of DA-9201, an extract of the black rice, in a mouse model of asthma. Mice immunized with ovalbumin (OVA) were administered with DA-9201 (30, 100 or 300 mg/kg) or dexamethasone (DEXA, 3 mg/kg) for 2 weeks and challenged with aerosolized OVA during the last 3 days. Anti-asthmatic effects were assessed by means of enhanced pauses, level of total lgE and Th2 cytokines in plasma or bronchoalveolar lavage fluid (BALF), the percentage of eosinophils in BALF, and histopathological examination. The expression of NF-$\kappa$B in nuclear and cytoplasmic fraction and its DNA-binding activity in lung tissues were analyzed by means of Western blotting and electrophoretic gel mobility shift assay (EMSA), respectively. DA-9201 significantly reduced airway hyperrespon-siveness (AHR), total lgE level in plasma and BALF, IL-4, IL-5, and IL-13 levels in BALF, and the percentage of eosinophils in BALF. Tissue inflammation was significantly improved by DA­9201 treatment. In addition, DA-9201 dramatically suppressed the expression of NF-$\kappa$B and its DNA-binding activity. These results suggest that DA-9201 may be useful for the treatment of asthma and its efficacy is related to suppression of NF-$\kappa$B pathway.

• Food Science and Preservation
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• v.16 no.3
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• pp.405-411
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• 2009

The manufacturing process and quality properties of Lactococcus lactis-fermented yakchobugak (LFY) containing some colored agro-food products were investigated to develop a good organoleptic bugak from the leaf of the medicinal herb. The de-oiling rate of LFY by centrifugation was 37.6%, which was significantly different to the 3.5% value obtained after standing and the 8.9% value obtained with the beating method (p<0.05). Reducing power (RS) increased with addition of increasing levels of ethanol extracts from agro-food powders. The RS of green tea extract-supplemented LFY had the highest value of 0.97 at $500{\mu}g/mL$, and this was significantly different to values obtained using other extracts (p<0.05). At 83.84%, the DPPH (2,2-diphenyl-1-picrylhydrazyl) free-radical scavenging activity of green tea extract-supplemented LFY was the greatest of all samples tested, followed by LFY with woolgeom at 39.48%, LFY with black rice at 28.45%, and LFY with bokbunja at 22.24% all ethanol extracts were added at $50{\mu}g/mL$ (p<0.05). Acid values of green tea and bokbunja LFYs stored in transparent PE bags at $60^{\circ}C$ for 7 days were 1.82% and 2.03%, respectively. Textural hardness values of LFYs were less than 250.62, except for LFYs with black rice and woolgeom, but these values increased $2{\sim}3$-fold after lactic acid fermentation. Carbohydrate and protein content of LFYs were in the ranges $58.95{\sim}64.63%$ and $7.50{\sim}8.68%$, respectively. Lipid and calorie contents of woolgeom LFY had the lowest values of 22.02% and 490 Kcal, respectively.

• Journal of the Korean Society of Food Science and Nutrition
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• v.37 no.5
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• pp.666-670
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• 2008

Lovastatin (Mevinolin, Monacolin K) is a well-known drug for the therapy of hypercholesterolemia. It is an important fungal secondary metabolite as it inhibits 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMG-CoA reductase, EC 1.1.1.34) which catalyzes a major rate-limiting step in the biosynthesis of cholesterol. Both soybeans and black soybeans with Aspergillus terreus ATCC 20542 mutant were used as the seed culture for the mass production of lovastatin. The production of lovastatin in soybean seed culture of Asp. terreus was twofold compared to that of black soybean seed culture. The effect of two different vessels (petri dish and Erlenmeyer flask) on lovastatin production was also studied. The production of lovastatin on petri dish was tenfold to that of Erlenmeyer flask. Furthermore, the most lovastatin production on rice bran was achieved when the soybean seed culture was treated by heat shock at $30^{\circ}C$ for 1 hour, representing 82% of HMG-CoA reductase inhibition in the koji extract. We estimated that the heat treated soybean seed culture could be a new method for the mass production of lovastatin.

• Journal of The Korean Society of Grassland and Forage Science
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• v.10 no.1
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• pp.42-47
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• 1990

Silage productivity of 7 corn and 3 sorghum hybrids was determined at two planting dates differing in rice black-streaked dwarf virus (RBSDV) infection rates in the southern part of Korea where RBSDV is prevalent. 1. Percent stand was similar between two planting dates, but that of corn hybrids (85.0-99.5 %) was higher compared with sorghum (59.8-82.3 %). Culm length of corn hybrids at April 23 planting was longer compared with May 20 planting, but that of sorghum hybrids was similar between two planting dates. 2. RBSDV infection rate of corn hybrids at April 23 planting was lower compared with May 20 planting, but sorghum was not infected at both planting dates. Among corn hybrids Suweon 19 was most severely infected, but Suweon 99 was least infected at both planting dates. 3. Percent ear bearing plants and earltotal dry matter (TDM) ratio of corn hybrids at April 23 planting was higher than those of May 20 planting. Among the corn hygrids percent ear beraring plants, ear/TDM ratio of Suweon 19 was lowest at both planting dates due to severe RBSDV infection. Ear/TDM ratio of sorghum hybrids was below 17.8 % due to sterility and partially bud damages. 4. Silage yield at April 23 planting was higher than that at May 20 planting and sorghum hybrids outyielded corn hybrids. Silage yield of Suweon 19 and Jinjuok was lowest due to severe RBSDV infection. 5. Crude protein was similar regardless planting dates, species, and varieties except the lowest P 931. Ether extract at April 23 planting was lower then that at May 20 and corn was lower compared with sorghum hybrids. Crude fiber and nitrogen free extracts(NFE) was similar between planting dates. Crude fiber of corn was lower, but NEF was higher compared with sorghum, but similar among hybrids in a species. 6. In vitro dry matter digestibility (IVDMD) at May 20 planting was higher than that of April 23 planting and corn was higher than sorghum hybrids. Digestible dry matter (DDM) of both corn and sorghum at April 23 planting was higher than that of May 20 planting. At April 23 planting DDM of all corn and sorghum hybrids was similar, but DDM of Suweon 19 and Jinjuok was lower than other corn and sorghum hybrids.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.34 no.1
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• pp.30-39
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• 1989

Forage productivity of cropping systems of rye - silage corn, silage corn - oats, silage corn - rape was studied in the south-eastern part of Korea where rice black-streaked dwarf virus(RBSDV) infection of corn are severe. Rye(cv. Paldanghomil) was planted on Oct. 20 of 1986 and harvested 10 times from April 5 to May 5 at the 5-day intervals in 1987, corn (cv. Suweon 19 and Jinjuok) was planted 5 times from April 5 to May 15 at the 10-day intervals in 1987, and oats(cv. Megwiri) and rape (cv. Velox) were planted 4 times from Sept: 4 to 25 at the 7-day intervals and harvested 4 times from Nov. 10 to Dec. 10 at the 10-day intervals in 1987. Considering yield, nutrition value, and in vitro dry matter digestibility (IVDMD), forage productivity of the cropping systems was compared. As harvesting time of rye delayed, plant height, dry matter(DM) yield, percent DM, crude fiber, and digestible DM yield increased, but crude protein, crude fat, and IVDMD decreased. However, nitrogen free extract was not different among the harvesting dates. As planting date of corn delayed, RBSDV infection rate increased. but DM yield of silage decreased. However, silage yield of Jinjuok was higher, but RBSDV infection rate was lower compared with Suweon 19 at all planting dates. DM yield of oats and rape decreased as planting date delayed. However, at Sept. 4 and 11 plantings yield of oats on Nov. 10 was much lower than that of rape, but the differences in yield between two crops decreased with delayed harvesting, and yield was similar on Dec. 10. A cropping system harvesting rye around April 20 and followed by planting corn in late April was best among the rye-corn systems considering yield and nutrition value of both crops. However, among the corn-oats or corn-rape cropping systems early April planting of corn and followed by early Sept. planting of oats or rape showed best results with similar yield potential of the best rye-corn cropping system.

• Korean Journal of Plant Resources
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• v.24 no.1
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• pp.105-112
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• 2011

The objective of this study was to determine the effect of antioxidant enzyme activity and radical scavenging activities of thirteen kinds of natural plant pigments. The analytic method of antioxidant activities were measured by estimating DPPH free radical scavenging and nitrite scavenging ability activity. The free radical scavenging activity by method using stable free radical DPPH was the highest in the red cabbaged pigment. Addition of ethanol extract 1mg/ml from onion peel pigment displayed remarkable effect on nitrite scavenging ability about 91.9%. Antioxidative enzyme activity was evaluated in terms of superoxide dismutase(SOD), catalase(CAT), and ascorbate peroxidase(APX) activity. The bitter melon pigment had the highest SOD activity of 87.3%. The activities of CAT and APX were higher in the mulberry leave pigment compared with other natural plant pigments. In contrast, CAT activity of plant pigment samples were unaffected. These results suggest that natural plant pigment had the potent biological activities such as antioxidant enzyme activities, and that their activities exhibited differently depending on each kind of pigments.

• Journal of The Korean Society of Grassland and Forage Science
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• v.11 no.2
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• pp.129-136
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• 1991

Dry matter productivity at silage stage of two corn hybrids varying resistance to rice blackstreaked dwarf virus(RBSDV) and two sorghum hybrids varying growth duration was evaluated at six planting dates (Apr. 1, Apr. 15, Apr. 29, May 13, May 27, and June 10) in Kyongsan where RBSDV is prevalent.Corn was harvested 38-40 days after silking. For sorghum 1st-cut was made 19-22 days after heading and some regrowth (2nd-cut) on October 13. 1. Percent stand of both corn hybrids was over 91% at all planting dates, but that of sorghum ranged 37.2-80.3% depending on hybrids and planting dates. 2. The number of days from planting to emergence decreased as planting date delayed in both crops. The number of days from emergence to silking of both corn hybrids and a sorghum hybrid(P 956) decreased as planting date delayed, but that of the other sorghum hybrid(P 931) increased significantly after May 13 planting. 3. RBSDV infection rate of corn hybrids was highest at April 1 planting (Kwanganok 24.896, Jinjuok 63.0%) and decreased up to April 29 planting and then increased as planting date delayed. Sorghum was not infected by RBSDV at all. 4. EarfTotal dry matter ratio of corn ranged 30.6-47.9% and that of sorghum 3.1-30.7% depending on hybrids and planting dates. All the hybrids developed grain normally at all planting dates except a sorghum hybrid (P 931) which developed few grains after May 13 planting. 5. Crude (C) protein and C. fat contents were similar among all treatments. C. fiber content of P 931 was higher than that of both corn hybrids and P 956, but nitrogen free extract (NFE) was lower. C. fiber and NFE of both corn hybrids and 1st-cut sorghum were similar among the planting dates, but C. fiber and NFE of 2nd-cut of sorghum decreased and C. protein increased as planting date delayed due to plants were younger. 6. Dry matter (DM) yield of all hybrids decreased as planting date delayed except Jinjuok of which yield was highest at April 29 planting. DM yield of corn was similar to 1st-cut sorghum, but lower than total DM yield of sorghum at April 1 and 15 plantings where regrowth yield was high.