• Plant Resources
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• v.2 no.1
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• pp.42-48
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• 1999

This study was undertaken to examine the feasibility of black locust (Robinia pseudoacacia) as substrates for several edible mushrooms. For the cultivation of several edible and/or medicinal fungi on black locust, optimum bulk densities, synthetic or semisynthetic additives, natural additives and pretreatment methods were investigated. Fruit body yields of the fungi on various sawdust media composed of different wood species were also analyzed for testing the capability of black locust as a substrate for mushroom production. Mycelial growths decreased proportional when the bulk density increased. The most suitable carbon and nitrogen sources as additives to promote the mycelial growth were sucrose (2%, w/w) and ammonium phosphate (0.2%, w/w) respectively. When corn-powder and beer-waste as natural additives were added to sawdust of black locust showed the significant growth of mycelia. And the optimum mixing ratio was 10:2:1 (sawdust: corn-powder: beer-waste, w/w). Black locust after cold water treatment showed the outstanding mycelial growths. Any significant changes of pH, moisture content (%) and dry-weight losses (%) could not be found among culture substrates (sawdust of black locust, oak and poplar wood) examined before and after harvesting of fruit bodies. Yield of fruit bodies on black locust culture media were comparable with those culture media composed with oak and poplar wood. The present work indicated strongly the potentiality of black locust as raw materials for edible and medicinal mushrooms.

• Current Research on Agriculture and Life Sciences
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• v.21
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• pp.39-47
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• 2003

The object of this study was to analyze influence which Black locust afforestation affected to soil, to research change of soil environment of Black locust planted stand and adjacent stands through comparative analyzing physichemical property of soil of them. It was investigated that the soil texture of Black locust planted stand and the adjacent stands was mostly SL. and LS. According to this result I could know that transformation of soil texture by vegetation easily happened because transformation soil texture results from the parent rock. I could ascertain that difference existed between Black locust planted stand and the other stands in soil pH and Carbon content of planted stand of Black locust pH, contents of Available $P_2O_5$ Carbon, Total Nitrogen. In other words, pH and Carbon content of Black locust planted stand were lower than that of Oak forest, higher than that of Pine forest. These results showed that Black locust was which fix nitrogen. I could infer that Black locust afforestation made forest soil fertilized. In soil of Black locust planted stand and the adjacent stands, statistical value appeared highly in pH and existed in content of Carbon, Total nitrogen, too. And high interrelationship appeared in the order of pH, C/N Ratio, CEC, Total nitrogen content was in each stands. I suppose that the trespassing extent of Black locust to Oak forest and Pine forest which are adjacent to planted stand of Black locust is different because of low pH, lackness of Available $P_2O_5$ Carbon, and what not.

• Korean Journal of Microbiology
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• v.3 no.2
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• pp.22-26
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• 1965

In order to investigate the host range of the mosaic disease of black locust in the Chunchon area, the sap of the mosaic-diseased leaves of black locust itself and the cowpea leaves infected with the above mentioned sap, were inoculated to 53 species of plants belong to 12 families. As to the result, no difference in infection was found as related to the virus sources, and the infection was recognized in 4 species of the family Chenopodiaceae and 8 species of the family Leguminosae. The plants recognized as hosts are as follows: the plants which showed local infection are Chenopodium album, Ch. ambrosioides, Ch. quinoa; the plants which showed systemic infection are Chenopodium amaranticolor, Phaseolus vulgaris, Robinia pseudo-acacia, Vigna sinensis; and Astragalus sinicus, Melilotus indicus, Phaseolus angularis, Pisum sativum and Vicia faba were recognized as carriers. Through investigating its host ranges and symptoms, this mosaic virus of black locust seems not to be regarded as the group of the black locust mosaic virus in southeastern Europe reported by Milinko et al (1961). And, too, it is thought hardly to exist in combination with the cowpea mosaic virus. It appears, therefore, that this mosaic virus was confined to that of black locust.

• Korean Journal of Organic Agriculture
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• v.9 no.3
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• pp.73-83
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• 2001

The qualities of the rice straw, black locust and orchardgrass silage added with grape pomace were investigated in this study and the amount of grape pomace added in different treatments were 0, 20, 40 and 50%, respectively. Crude protein contents of rice straw(6.5-10.6%) and orchardgrass silage(13.2-14.8%) added with grape pomace were significantly higher(p<0.05) than that of 100% rice straw(5.3%) and orchard grass silage(12.8%), however the crude protein contents(14.9-15.6%) of black locust silage in supplementation were lower than that of 100% black locust silage(16.3%). With higher amount of addition of grape pomade to rice straw, black locust and orchardgrass silages, ADF, NDF and crude ash contents decreased significantly(p<0.75). Moisture contents decreased according to the higher proportion of grape pomace in the rice straw silages, but increased according to the higher proportion of grape pomace in the black locust and orchardgrass silage. Values of pH in 20∼60% addition of grape pomace in rice straw(4.0∼4.2), black locust(4.1∼4.3) and orchardgrass silages(4.2∼4.4) were lower than that of 100% in rice straw(4.6), black locust(5.4) and orchardgrass silages(4.7). The contents of acetic acid and total volatile fatty acid significantly increased according to higher levels of addition of grape pomace compared to the respective values of silage. However the contents of lactic acid in supplementation of grape pomace were lower than that of 100% black locust silage.

• Korean Journal of Organic Agriculture
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• v.9 no.4
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• pp.123-134
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• 2001

The quality including in situ degradability in the rumen of Holstein of the black locust silage added with apple pomace was investigated in this study. The amount of apple pomace added in different treatments were 0, 20, 40 and 60% respectively. With higher amount of addition of apple pomace in black locust, crude protein content in the silage decreased. Crude protein contents in the apple pomace added silage were in the range between 11.7 and 13.7% and those were significantly lower than 16.3% of 100% black locust silage. The contents of ADF, NDF and crude ash were in the same trend as with crude protein, which were shown more clearly in the 40∼60% addition of apple pomace. Moisture content of 100% black locust silage was 56.7% however it significantly increased according to higher levels of addition of apple pomace(67.1∼73.8%). pH, lactic acid and total organic acid contents in 20∼60% addition of apple Pomace were 3.7∼4.3, 1.3∼2.2%, and 2.1∼6.0% respectively, however in 100% black locust silage those were significantly lower except pH as 5.4, 0.6% and 1.0% respectively. In situ disappearance rates of dry matter and NDF in the rumen were significantly higher at the stapes of incubation after 12h and 24h respectively in 20∼40% addition of apple pomace than in 100% black locust silage. No statistical differences were observed with quickly degradable fraction (a) in the disappearance rates of dry matter and NDF. In dry matter, however slowly degradable fractions (b) of 100% black locust and 60% addition of apple pomace were significantly higher as 99.7 and 99.8% respectively than 37.7∼50.5% of 20∼40% addition of apple pomace. On the contrary, fractional rate of disappearance (c) and effective degradability(ED) were significantly higher in 20∼40% addition of apple pomace as 0.0115∼0.0149 and 30.4∼31.9% respectively than the respective values of 0.0027 and 24.9% of 100% black locust. In NDF, b was significantly higher in apple pomace added silage(38.5∼99.8%) than in 100% black locust silage(14.9%). However, C was significantly lower in apple pomace added silage than in 100% black locust silage.

• Journal of Life Science
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• v.9 no.1
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• pp.50-53
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• 1999

Effects of growth regulators and medium components were tested for shoot multiplication and callus growth from shoot explants of black locust. During shoot multiplication, callus growth at the cut end of shoot explants proceeded shoot development. The basal callus growth seemed to be a function of both mineral components and cytokinin supplemented in the medium. Maximum callus growth was induced by 0.5${\mu}$M BAP and the callus growth decreased as the level of BAP increased. Positive correlations were found between basal callus growth, and shoot multiplication and growth. Shoot multiplication was greatest on BSM medium (black locust shoot culture medium) supplemented with 1 $\mu$M BAP. With medium containing high nitrogen content, both shoot multiplication and growth were significantly enhanced. A new BRM medium was the most effective for rooting of black locust among three rooting media tested.

• Journal of the Korean Wood Science and Technology
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• v.23 no.3
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• pp.73-82
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• 1995

Black locust trees(Robinia pseudo-acacia L.) are growing widely in Korea and have been used to get honey and animal feed, but they have seldom been used as manufacturing materials. This study was carried out to evaluate the possibility of using black locust as bent-wood furniture materials. Softening methods were steaming and micro-wave heating. The specimens of 15${\times}$15${\times}$350mm were all air-dried. For softening, specimens were steamed at 100$^{\circ}C$ for 7 min, or microwave heated for 35 seconds. It is known that microwave heating softens wood much faster than steaming. The bending processing conditions are showed in Table 7. The minimum solid-bending radii of black locust were 40mm for steaming and 150mm for micro-wave heating, respectively. In conclusion the steamed black locust, showed very good solid bending properties, but the bending properties of micro-wave heated black locust were not sufficient for bent-wood furniture.

• Journal of Korean Society of Forest Science
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• v.94 no.5 s.162
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• pp.291-301
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• 2005

Robinia pseudoacacia is one of most widely cultivated exotic species in Bulgaria. The total area of black locust plantations amounts to 2.9% of the total forest area of the country. 15.34% of the plantations are of management afforestations category, where the priority is given to timber production. They have been created on rich and moisture soils, which are the most appropriate for the species in order to achieve its biological potentials of high productivity. The rest of the available plantations in the country are planted on poorer and drier soils up to 600~800 m altitude. The high adaptive ability of the species to unsuitable environmental conditions as well as the high sprout potential was used for their creation. These stands are mainly done with the aim to protect and ameliorate damaged environments and production of small-size timber and fire woods. They are cultivated until 15-20 years and are revived by sprouts. Therefore the management goals searched, 45.69% of those forests are low productive and 38.97% with average productiveness. The present report deals with growth and productivity capacity of black locust plantations; production of sowing materials; production of reproductive and vegetative saplings for afforestation; the technological aspects of afforestation works; the management and use of black locust plantations as well as some more important diseases and pests affecting the species. The report is entirely based on Bulgarian scientific research works and experience with the Robinia preudoacacia. Recommendations for optimizing the use of species are given.

• Korean Journal of Microbiology
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• v.4 no.2
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• pp.19-21
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• 1966

For the purpose of determining possibility of aphid-transmission of mosaic disease of black locust, cowpea aphid (Aphis medicagnis Koch) and green peach aphid (Myzus persicae Sulzer) were experimented using cowpea as test plant, and both proved to be the vectors. As for transmission threshold period of cowpea aphid to the virus, the acquisition feeding period was five seconds and inoculation feeding period was two minutes. This black locust mosaic virus, therefore, is a nonpersistent virus.

• Journal of Korean Society of Forest Science
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• v.96 no.6
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• pp.737-741
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• 2007

Typical phytoplasma witches' broom symptoms were observed in black locust (Robinia pseudoacacia L.) in Korea. The symptoms of the disease were showing abnormally small leaves, shortened intemodes and proliferation of shoots. The phytoplasmas were detected consistently in all the symptomatic samples by the amplification with phytoplasma universal primer pairs P1/P7 and R16F2n/R2, and the expected size was 1.8 kb and 1.2 kb. However, the phytoplasma DNA was not detected in healthy seedling. Based on sequence analysis of amplified region, this phytoplasma has close homologies with aster yellow, mulberry dwarf, maize bushy stunt, ash witches' broom and sumac witches' broom phytoplasmas, more than 99.2% but showed homologies with black locust witches' broom (GeneBank Accession No. AF 244363), and jujube witches' broom, 88.6% and 87.7%, respectively. This phylogetic analysis indicates that the black locust witches' broom phytoplasma founded in korea should be classified in the Candidatus phytoplasma asteris (16Sr I) group and clearly distinct from the black locust witches' broom group 16Sr III (peach X-disease phytoplasma group).