• 한국임상수의학회지
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• 제28권6호
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• pp.566-570
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• 2011

본 연구에서는 난소자궁적출술에 있어서 티타늄(titanium) 클립 적용 시 발생할 수 있는 합병증을 평가하고, 난소혈관과 난소걸이인대의 두 가지 결찰법을 비교하여 합병증을 최소화 할 수 있는 수술법을 찾고자 하였다. 실험을 위해 6마리의 암컷 비글견이 사용되었으며, 세 마리씩 두 그룹으로 나누어 한 그룹은 난소걸이인대와 난소혈관을 함께 결찰 하였고, 나머지 한 그룹은 난소걸이인대를 결찰하지 않고 난소혈관만 결찰 하였다. 두 수술법을 비교하기 위해서 매달 1회씩 1년 동안 복강 X-ray 검사를 통해 적용된 클립의 위치 변화를 확인하였고, 적용된 클립에 의한 염증반응 유무는 혈액검사를 통해 확인하였다. X-ray 검사 상에서 두 그룹의 클립 위치는 큰 변화를 보이지 않았으며, 혈액 검사에서도 거의 모든 수치들이 정상범위에 있었고, 염증과 관련된 임상증상 또한 관찰되지 않았다. 부검을 통한 두 그룹의 클립 포매 형태를 관찰한 결과 난소걸이인대를 결찰한 클립은 복강의 얇은 막으로 두껍게 싸여서 신장 후연에 위치하고 있는 것을 확인하였고, 난소걸이인대를 결찰하지 않은 클립은 얇게 둘러싸인 막 표면에 위치하여 신장의 아래쪽에서 관찰되었다. 이러한 결과는 난소걸이인대가 클립의 움직임을 최소화 할 수 있도록 지지해주는 역할을 하는 것을 의미하며, 따라서 티타늄 클립을 적용하는 난소자궁적출술에서 난소걸이인대를 함께 결찰하는 방법이 합병증 예방에 있어서 보다 적합한 방법으로 생각된다.

• 한국임상수의학회지
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• 제18권2호
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• pp.139-145
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• 2001

This study was undertaken to determine the normal serial ultrasonographic appearance of the postpartum uterine involution in small pet dogs (Yorkshire terrier and Maltese). Postpartum changes in uterine shape, architecture, echogenicity and diameter were monitored by ultrasonography in 12 small pet dogs. Serial ultrasonographic examinations were done daily during the first week, 3 days interval from 8 to 30 days, and weekly from 31 to 100 days postpartum. The excretory period of vaginal discharge in 12 normal bitches of uterine involution was finished completely within 3 weeks postpartum. The short axis shape of the uterus was initially often flaccid-appearing. It varied from circular to polygonal. This lasted until 15.75$\pm$3.84 days postpartum, during which time the short axis uterine shape gradually changed to circular. Also, the long axis shape of the uterus was a beaded appearance until 30.89$\pm$4.25 days postpartum. After 30 days, it was appeared as tubular shape between placental and interplacental sites. The ultrasonographic image of the postpartum uterus consisted of four echogenicity distinct layers. Uterine wall was represented as very hyperechoic serosa, hypoechoic myometrium, hyperechoic endometrium and anechoic structures of fluid in the uterine cavity until 7 days postpartum. The individual uterine layers were most prominent during the first week postpartum, and they became progressively less distinct throughout the course of uterine involution. The thickness of myometrium was decreased rapidly in the placental sites from 4.47$\pm$1.42 mm at 1 day to 1.92$\pm$0.26 mm at 16 day, and in the interplacental sites from 3.19$\pm$0.61 mm at 1 day to 1.39$\pm$0.61 mm at 16 day. And it was decreased slowly until 94 day and was been minimum thickness at 94 day. The thickness of endometrium was also decreased like that of myometrium. The uterine diameter in the placental sites was decreased from 22.28$\pm$3.01 mm at 1 day to 16.11$\pm$1.46 mm at 7 day, and in the interplacental sites was decreased from 13.65$\pm$2.34 mm at 1 day to 9.41$\pm$1.59 mm at 7 day postpartum. From 7 day to 93 day, the change of diameter was more and more slow. At 94 days postpartum, the uterine diameter was 5~6 mm both placental and interplacental sites, and the uterine horns were uniform hypoechoic, tubular structures without enlargement. Therefore, complete involution of the uterus occurred at 94 days. It was concluded that normal post partum uterine involution in small pet dogs appeared to be completed 94 days postpartum by gross findings such as vaginal discharges, and by ultrasonographic findings, uterine shape and echogenicity.

• 한국수정란이식학회지
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• 제28권3호
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• pp.237-241
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• 2013

It is generally accepted that chronic stress impairs female reproduction. It negatively affects ovarian function and the number of ovulated oocytes. Chronic stress lowers the number of retrieved oocytes. Ovarian follicular development is regulated by both pituitary-derived gonadotropins and intraovarian regulatory factors. The main corticosteroids are cortisol, cortisone, 11-deoxycortisol and corticosterone, cortisol being one of the most commonly used welfare and stress physiological indicator. In this study, we investigated the effect of cortisol level on progesterone patterns and ovulation in the dog. Cortisol and progesterone level of serum were analyzed by radioimmunoassay. The day of ovulation was considered as the day when serum progesterone concentration was 6.0~8.0 ng/ml. In vivo dog oocytes were collected by flushing oviducts of mixed-breed bitches at three days after ovulation. We classified dogs as having group 1 (cortisol level, 0 ${\leq}$ or < $2{\mu}g/dl$), group 2 (corisol level, 2 ${\leq}$ or < $4{\mu}g/dl$), group 3 (cortisol level, 4 ${\leq}$ or < $6{\mu}g/dl$) and group 4 (cortisol level, $6{\mu}g/dl$ ${\leq}$). The patterns of progesterone were not different in four cortisol groups. The average numbers of retrieved oocytes was not different in four cortisol groups. These results suggest that different cortisol levels on estrus dogs do not affect ovulation, number of ovulated oocytes and progesterone changes.