• Korean journal of applied entomology
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• v.43 no.4 s.137
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• pp.281-289
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• 2004

The sex pheromone blend (28-12AC : E8-12AC : Z8-12OH = 95 : 5 : 1) of oriental fruit moth, Grapholita molesta (Busck), was evaluated with reference to its male attractivity at different dosage and lure longevity of 1.0 mg in a field condition. Also, degree-days (DD) were calculated up to major phenological events of G. molesta using seasonal adult flights monitored by pheromone traps and egg population densities in early season in Suwon peach orchards. In the range of 0.1-4.0 mg dosage examined, G. molesta males caught in pheromone trap decreased with increasing total pheromone doses. The highest number of G. molesta males were attracted in traps baited with 0.1 mg-lure, and there were no significantly differences among 0.5 to 4.0 mg-lure. In longevity test with a 1.0 mg-lure, there was no difference in mean numbers of G. molesta males caught between traps with fresh-baited lures every 20d and field-lasted lures up to ${\approx}50d$ after trap installation, but thereafter more G. molesta males were attracted in the field-lasted lures than in the fresh-baited lures. Accumulated degree-days (DD) from January 1 to the first adult emergence (biofix) were 39DD at a lower threshold temperature $8.1^{\circ}C$. Degree-days from the biofix up to the 1st adult peak through 4th peak were 98DD, 620DD, 1233DD, and 1916DD, respectively. Required degree-days from the 1st adult peak to the 1st egg peak were 130DD, while the 2nd egg peak timing was simultaneous with the 2nd adult peak. Further, management strategies of G. molesta were discussed in peach orchards.

• Journal of Plant Biotechnology
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• v.45 no.3
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• pp.171-182
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• 2018

Papaya (Carica papaya L.) is one of the crops widely planted in tropical and subtropical areas. The papaya fruit has low calories and are plentiful in vitamins A and C and in minerals. A major problem in papaya production is a plant disease caused by the papaya ringspot virus (PRSV). The first PRSV-resistant GM papaya expressing a PRSV coat protein gene was developed by USA scientists in 1992. The first commercial GM papaya cultivars derived from the event was approved by the US government in 1997. Development of transgenic papayas has been focused on vaccine production and limited agricultural traits, including insect and pathogen resistance, long shelf life, and aluminum and herbicide tolerance. Approximately 17 countries, including the USA and China, produced transgenic papayas and/or commercialized them, which provoked studies on biosafety assessment and development of GM-detection technologies. For the biosafety assessment of potential effects on human health, effects of long-term feeding to model animals have been studied in terms of toxicity and allergenicity. Studies on environmental safety assessment include influence on soil-microbial biodiversity and transfer to soil bacteria of GM selection markers. Many countries, such as Korea, the European Union, and Japan, that have strict regulations for GM crops have serious concerns about unintended introduction of GM cultivars and food commodities using unauthorized GM crops. Transgene- and/or GM event-specific molecular markers and technologies for genomics-based detection of unauthorized GM papaya have been developed and have resulted in the robust detection of GM papayas.

• Molecules and Cells
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• v.38 no.3
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• pp.210-220
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• 2015

Athletic performance is an important criteria used for the selection of superior horses. However, little is known about exercise-related epigenetic processes in the horse. DNA methylation is a key mechanism for regulating gene expression in response to environmental changes. We carried out comparative genomic analysis of genome-wide DNA methylation profiles in the blood samples of two different thoroughbred horses before and after exercise by methylated-DNA immunoprecipitation sequencing (MeDIP-Seq). Differentially methylated regions (DMRs) in the pre-and post-exercise blood samples of superior and inferior horses were identified. Exercise altered the methylation patterns. After 30 min of exercise, 596 genes were hypomethy-lated and 715 genes were hypermethylated in the superior horse, whereas in the inferior horse, 868 genes were hypomethylated and 794 genes were hypermethylated. These genes were analyzed based on gene ontology (GO) annotations and the exercise-related pathway patterns in the two horses were compared. After exercise, gene regions related to cell division and adhesion were hypermethylated in the superior horse, whereas regions related to cell signaling and transport were hypermethylated in the inferior horse. Analysis of the distribution of methylated CpG islands confirmed the hypomethylation in the gene-body methylation regions after exercise. The methylation patterns of transposable elements also changed after exercise. Long interspersed nuclear elements (LINEs) showed abundance of DMRs. Collectively, our results serve as a basis to study exercise-based reprogramming of epigenetic traits.

• Journal of Life Science
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• v.19 no.10
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• pp.1417-1423
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• 2009

Previous studies have suggested that docosahexaenoic acid (DHA) supplementation into n-3 fatty acid deficient diet improved spatial learning performance, but there was no significant difference in brain related function when DHA was added into a n-3 fatty acid adequate diet. Here, we investigated the effect of adding DHA into an n-3 fatty acid deficient or adequate diet on brain and liver fatty acid composition. On the second day after conception, Sprague Dawley strain dams were divided into four groups as follows; n-3 fatty acid deficient (Def), n-3 fatty acid deficient plus DHA (Def+DHA, 10.2% DHA), n-3 fatty acid adequate (Adq, 3.4% linolenic acid), and n-3 fatty acid adequate plus DHA (Adq+DHA, 3.31% linolenic acid plus 9.65% DHA). After weaning, male pups were fed on the same diets of their respective dams until adulthood. In brain fatty acid composition, the Def group showed a lower brain DHA (64% decrease), which was largely compensated for by an increase in docosapentaenoic acid (22:5n-6). Brain DHA in the Def+DHA group was increased to almost the same extent as in the Adq and Adq+DHA groups and there were no significant differences among them. Liver fatty acid composition showed a similar pattern to that of the brain, but liver DHA in the Def+DHA showed the highest percentage among the diet groups. In conclusion, n-3 fatty acid deficiency from gestation to adulthood leads to decreased brain DHA, which has been shown to be highly associated with poor spatial leaning performance. Thus, adequate brain DHA levels are required for optimal nervous function.

• The Korean Journal of Food And Nutrition
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• v.22 no.1
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• pp.48-56
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• 2009

This study was conducted to examine the effects of Opuntia ficus-indica complexes(OCB) on the intake of water and food, body weight, blood glucose levels, glucose tolerance and lipid metabolism in streptozotocin(STZ)-induced diabetic rats. Thirty-two male Sprague-Dawley rats were assigned to four different groups; non-diabetic control(NC), diabetic control (DC), diabetic OCB of 2%(OCB-2), and diabetic OCB of 5%(OCB-5). The animals were fed on each experimental diet for 3 weeks. The DC, OCB-2 and OCB-5 groups showed a higher intake of water and food than the NC group. The fasting blood glucose levels were 100 $ mg/d{\ell}$ and 416 $ mg/d{\ell}$ for the NC and DC groups, respectively. The OCB-5 group presented a significantly low fasting glucose level of 21%(P<0.05), while OCB-2 group had a decrease of 13% compared to the DC group. As for the results of the glucose tolerance test, the highest blood glucose level was observed for all the groups at 30 minutes after the glucose injections as well as higher plasma insulin levels in the OCB-5 group. Plasma total cholesterol, triglyceride, non-esterified fatty acids(NEFA) and LDL-cholesterol concentrations were also lower in the OCB-2 and OCB-5 groups. The experimental diet did not affect the HDL-cholesterol levels. The overall results suggest that the higher intake of food by the OCB-2 and OCB-5 groups improved the blood glucose levels and lipid metabolism in STZ-induced diabetic rats.

• The Korean Journal of Food And Nutrition
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• v.22 no.3
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• pp.387-395
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• 2009

This study was conducted to investigate the effects of starch concentrations and heating conditions on the gel characteristics of arrowroot starch. Arrowroot starch gels with various pHs, and starch concentrations, were prepared using different temperatures and heating times, and then stored for 24 hrs at $4^{\circ}C$. The hardness of sample gels made at pH 2.0 and 4.0 increased as the starch concentration increased from 7% to 10%, with the maximum value of 94 N being obtained when the gel was prepared at pH 4.0 with a starch concentration of 10%. The maximum hardness of samples prepared with concentrations of starch ranging from 7~9% appeared at $80^{\circ}C$, regardless of the heating temperature and time. Furthermore, the hardness of samples prepared at greater than $100^{\circ}C$ was relatively lower than that of samples prepared at other temperatures. When a starch concentration of 8% was used, the degree of gelatinization(DR) increased as the heating temperature increased, with the maximum value of DR being about 76% at $120^{\circ}C$, regardless of heating time. After storage for 24 hrs, the hardness of samples prepared at $70^{\circ}C$, $80^{\circ}C$ and $90^{\circ}C$ appeared to decrease, while that of samples prepared at $100^{\circ}C$, $110^{\circ}C$ and $120^{\circ}C$ increased. The correlation between hardness and the degree of gelatinization or retrogradation was very high when samples were prepared at $80^{\circ}C$ with a starch concentration of 9%, as indicated by a correlation coefficient of greater than 0.95. Overall, the microstructures of freeze-dried arrowroot starch gel were composed of a continuous network of amylose and amylopectin with fragmented ghost structures in an excluded phase, but these ghost structures were more evident after storage and with increased heating temperature.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.50 no.spc1
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• pp.69-76
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• 2005

This study was carried out to improve the rice grain quality of Gyeoungbuk Province from 2002 to 2004. In variation of grain quality characteristics as the cultivation years were changed, the coefficient of variation (CV) of palatability and amylose content were relatively low as $3.9\~4.3\%$ and those of protein content and head rice rate were high as $7.9\~12.2\%$. Among the varieties, the tendency of variation was similar with cultivation years changing, CV of amylose content and palatability also low as $2.6\~3.6\%$ and those of head rice rate and protein content were high as $5.4\~7.2\%$. In variation as affected by shifting of transplanting times, the CV of protein content was low as 2.2, it was also relatively low in amylose content and head rice rate as $3.1\~3.7\%$, but it was high in palatability as $5.8\%$. As the nitrogen application levels were different the CV of amylase content was $1.8\%$ that it was not affected by the N levels. But in case of protein content, the CV was $4.4\%$ that the variation was somewhat increased, it suggested that as the N levels were increased the protein content was also increased. From these results, the rice quality characteristics showed the higher variation in the change of cultivation years than that in rice varieties, transplanting times or nitrogen levels.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.63 no.3
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• pp.257-264
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• 2018

The recent global climate change induced the drought, flooding, and insect pest outbreaks. These caused the severe damage to crop yield in the domesticated field and occurrence change of insect pest species. The sap-sucking insect pest, aphids are common in soybean [Glycine max (L.) Merr.] and cause serious yield losses. Thus, developing resistance cultivars is promising and efficient strategy to prevent the significant yield losses by aphid and screening germplasm is the essential procedure to achieve this goal. We tried to establish a resistance test indicator for foxglove aphid, Aulacorthum solani (Kaltenbach), in soybean and found that plant damage degree or infested plant damage is most suitable one. Also we screened around 1,200 of soybean germplasm including wild and cultivated species for its resistance to foxglove aphid from the various origins, and 67 soybeans, including PI 366121, showed antixenosis resistance, 31 germplasms among 67 antixenosis germplasms were showed antibiosis to foxglove aphid with non-choice test. The identified foxglove aphid resistant soybean resources showed significantly low rate in survival test. Furthermore, resistance type, (i.e., antibiosis or antixenosis) of each candidate were varied. In this research, we established the screening index for foxglove aphid resistance in soybean, and identified the resistance varieties. This result could be useful resources in breeding for new foxglove aphid resistance soybean cultivars, and provide fundamental information to investigate the resistance mechanism in soybean.

• Journal of Dairy Science and Biotechnology
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• v.33 no.1
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• pp.1-15
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• 2015

Before the advent of pasteurization and other microbiological controls in the dairy industry, milk product-borne diseases such as scarlet fever, typhoid fever, septic sore throat, and tuberculosis were globally widespread. Pasteurization was invented by Louis Pasteur, and it has been considered as one of the most effective ways to control milk product-borne diseases since the 20th century. Nevertheless, till date, various types of cheese in the EU, the USA, and other countries are made from unpasteurized milk as artisan cheese, following the specific food regulations of each nation. Furthermore, after the effectiveness of the Free Trade Agreement (FTA) negotiations between Korea and many nations, the import of various types of cheese made in different conditions and influenced by acidity, preservatives, temperature, competing flora, water activity, and salt concentration increased yearly. Hence, the objective of this review was to describe (1) the 60-day aging rule of cheese, (2) characteristics of the outbreaks linked to cheese manufactured from unpasteurized milk since 1998 to 2011 in USA, and (3) serious health risks from unpasteurized milk, to ensure food protection and safety and to use this basic information for risk assessment.

• Journal of Dairy Science and Biotechnology
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• v.33 no.1
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• pp.35-46
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• 2015

Recently, much attention has been paid to the development of a value-added food category containing probiotics so as to improve human health and prevent diseases. Among various foods, the health benefits of milk and dairy products are known to humanity, and could be attributed to the bioactive components present in milk. In fermented milk products, the health benefits could be due to suitable modulation activities produced by the action of probiotic bacteria. Besides the modification of various milk components, probiotics might also act directly as preventive and therapeutic agents against some severe diseases. Probiotics promote health via their positive effects on the immune response, stimulation of natural immunity, and modulation of the production of antimicrobial peptides, cytokines, and so on. Whey proteins, a byproduct of cheese production could also have anticarcinogenic, immunostimulatory, antimicrobial, and health-promoting activities such as improving insulin sensitivity and reducing fat deposition. Therefore, milk and dairy products containing probiotics could provide various opportunities in the field of functional foods. Additionally, these functional foods may be important in the human diet and may help improve human health and prevent diseases.