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Effects of Mume Fructus on the Rat Hypothyroidism Induced by PTU(6-n-propyl-2-thiouracil) (PTU(6-n-propyl-2-thiouracil)로 유발된 Rat 갑상선 기능저하증에 미치는 烏梅의 효과)

  • Choi, Jae Young;Roh, Seong Soo;Park, Ji Ha;Koo, Jin Suk;Seo, Bu Il
    • The Korea Journal of Herbology
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    • v.30 no.4
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    • pp.109-119
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    • 2015
  • Objectives : The aim of this study was to evaluate the effect of aqueous extracts of Mume Fructus(MF) on the 6-n-propyl-2-thiouracil(PTU)-induced rat hypothyroidism.Methods : Aqueous extracts of MF(yield = 19.38%) were administered, once per day for 42 days from 2 weeks before starting of PTU treatment as an oral dose of 300 and 150 ㎎/㎏(body weight), and hypothyroidism was induced by daily subcutaneous treatment of PTU 10 ㎎/㎏ for 28 days. The changes in the body weight, thyroid gland weights, liver weight, serum levels of thyroid hormone-thyroid stimulating hormone(TSH), tri-iodothyronine(T3) and thyroxine(T4), total cholesterol, low density lipoprotein(LDL), high density lipoprotein(HDL) and triglyceride (TG), aspartate aminotransferase(AST), alanine aminotransferase(ALT), liver antioxidant defense system-lipid peroxidation, H2O2, superoxide dismutase(SOD) and catalase(CAT) were examined with histopathology of thyroid glands and liver.Results : Results were compared with LevoT40.5 ㎎/㎏ treated rats. MF extracts recovered from the decreases in the body weight, liver weight, T3and T4, TG, liver CAT activities as results of PTU treatment. And MF extracts recovered from the increases of thyroid gland weights, TSH, HDL contents, liver H2O2, AST as results of PTU treatment. In addition, these PTU-induced histopathological changes in thyroid glands and liver related to hypothyroidism were dramatically decreased by treatment of both different dosages of MF extract, respectively.Conclusions : According to the above results, it is suggested that MF extracts have advantageous effects on the thyroid hormone productions with beneficial effects on the hypothyroidism related liver injuries mediated by the modulation on the antioxidant system.

Changes in Chemical Components and Antioxidant Activity of Dried Jujube with Different Aging Temperatures and Durations (가온숙성온도와 기간에 따른 건대추의 성분과 항산화활성 변화)

  • Park, Hye-Jin;Lee, Sang-Hoon;Kim, Hyun-Young;Jang, Gwi-Yeong;Hwang, In-Guk;Woo, Koan-Sik;Kwon, Oh-Sung;Lee, Jun-Soo;Jeong, Heon-Sang
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.41 no.5
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    • pp.591-597
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    • 2012
  • This study was conducted to investigate the changes in chemical components and antioxidant activities of dried jujube ($Zizyphus$ $jujuba$ Miller) with different aging temperatures ($60^{\circ}C$, $70^{\circ}C$, and $80^{\circ}C$) and durations (5 days, 10 days, 15 days, 30 days, and 50 days). The browning index increased from 0.124 to 1.998 with increased aging temperature and duration. As aging duration increased, fructose, glucose, and sucrose content decreased from 24.96% to 4.96%, from 22.23% to 4.41%, and from 18.41% to 0%, respectively. Reducing sugar content decreased from 351.52 mg/g to 155.96 mg/g as aging temperature and duration increased. Total acidity slightly increased from 1.61 to 3.13%. 5-Hydroxymethyl-2-furaldehyde increased from 0.002 mg/g to 38.84 mg/g, respectively. As aging temperature and duration increased, total polyphenol content increased from 15.98 mg/g to 38.61 mg/g. DPPH and ABTS radical scavenging activities increased from 4.03 mg trolox eq/g to 22.22 mg trolox eq/g, and from 2.11 mg ascorbic acid eq/g to 13.39 mg ascorbic acid eq/g, respectively. Reducing power increased rapidly in the first stage and then slowly increased afterward.

Assessment of Environmental Contaminant Levels in Water, Sediments and Adjacent Soils and Spider from Several Stream Sites in Korea (국내 하천들의 수질, 하천 퇴적물, 주변 토양 및 별늑대거미 체내 중금속 축적량을 통한 환경오염도 평가)

  • Byun, Sang-Hyuk;Ryoo, Keon-Sang;Choi, Jong-Ha;Kim, Yong-Gyun;Lee, Hwa-Sung
    • Journal of Environmental Science International
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    • v.17 no.3
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    • pp.287-296
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    • 2008
  • A comprehensive quality survey for heavy metals, organophosphorous and organochlorine residual pesticides, and coplanar PCBs in samples such as water, sediments and soils as well as spiders has been implemented. The samples were undertaken at nine stream sites and their vicinity in August 2006, representing different surrounding environments. The levels of PCBs were expressed as concentrations and WHO-TEFs. Among 12 coplanar PCBs as target compounds in this study, 2,3', 4,4', 5-PentaCB (IUPAC # 118) was the congener with the highest concentration. The total concentrations and TEF values of coplanar PCBs in Siheung stream sediment (heavy industrial complex site located in Ansan city) were 3915.50 pg/g and 0.8366 pg-TEQ/g on a dry weight basis, respectively. Such levels were around 40 times higher compared to sediment from Gapyung stream (green site located in the upper of Myunggi mountain). It is probably due to the direct input of PCBs trom PCBs treatment materials. Organophosphorous (EPN, dementon-s-methyl, diazinon, parathion, and phenthoate) and organochlorine (alpha-BHC, aldrin, 4,4'-DDT, 4,4'-DDE, endosulfan alpha and etc.) pesticides were not detected above 5 ppb of detection limits. The concentrations of Cu and Cd in water and sediment samples from Siheung stream were 44.11 and $0.17ug/m\ell$ and $713.42{\mu}g/g$ and $3.73{\mu}g/g$, respectively, which contained $20\sim40$ times higher concentrations than those from Gapyung stream. In addition to the water and sediment samples, the levels of heavy metals in spider from designated sampling sites were also determined. Heavy metals in spider collected near Siheung stream was appeared to be equivalent and/or a little higher levels with respect to other spiders. Furthermore, the ratio of relative heavy metals (Cu, Cd, and Pb) in spider from each stream site showed a correlation as similar as that of heavy metals in soil samples.

Comparative Analysis to Damage Reduction of Host Plant by Applying a Mating Disruptor of the Oriental Fruit Moth, Grapholita molesta in Two Different Cultivation Environments of Apple Orchard (재배환경이 상이한 사과원에서 복숭아순나방(Grapholita molesta) 교미교란제 처리에 의한 기주 피해 억제 효과 비교)

  • Jung, Sung-Chae;Kim, Yong-Gyun
    • Korean journal of applied entomology
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    • v.47 no.1
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    • pp.51-57
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    • 2008
  • This study demonstrates a variability in efficacy of mating disruption against the oriental fruit moth, Grapholita molesta, populations infesting apples cultivated in differential environmental conditions. Throughout the growing seasons, trap catches of G. molesta male moths and damaged leaf and fruit were examined to evaluate the efficacy of a commercialized mating disruptor. Treatment of the mating disruptor significantly reduced the trap catches of male moth in treated plot, compared to those of the untreated plot. This reduced trap catches were significantly correlated with leaf and fruit damage. Moreover, the highest host damage occurred in June just after the highest overwintering adult peaks in both plots. The treatment of mating disruptors in Chungsong effectively disrupted the overwintering population in April and May, resulting in no noticeable host damage during the following growing seasons. However, there was a marked difference in host damage between two plots, especially in late seasons. Variation in the efficacy of mating disruption technology in terms of host damage appeared to be related with nearby pheromone-untreated orchards, which may result in the immigration of gravid females.

Effects of Timings and Light Intensities of Supplemental Red Light on the Growth Characteristics of Cucumber and Tomato Plug Seedlings (적색광 처리시기 및 광도가 오이 및 토마토 플러그묘의 생장에 미치는 영향)

  • Zhang, Cheng-Hao;Chun, Ik-Jo;Park, Yong-Chul;Kim, Il-Seop
    • Journal of Bio-Environment Control
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    • v.12 no.4
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    • pp.173-179
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    • 2003
  • This study was established to control the overgrowth of cucumber and tomato plug seedlings by controlling of irradiation timings light intensities of red (R) light. Compared with the control, end-of-day (EOD) timing of R irradiation significantly reduced the seeding heights by 21.3% and 14.2% in cucumber and tomato, respectively. In addition, both plant seedlings treated with R light at EOD timing had the thickest stem diameter. Tomato seedlings treated with R light at EOD showed th highest chlorophyll content and the smallest leaf size. Dry weights of above ground plant tissue in both plant seedlings were significantly reduced by EOD R light treatment. And both plant seedlings treated with EOD treatment had the lowest T/R ratio, and the highest compactness rates. The increased intensities of R light resulted in the shorter plant heights in cucumber and tomato plant seedlings. It also reduced the length of hypocotyls and internodes and the size of leaves in the both seedlings. Elevated R light intensities tended to increase the stem diameter and chlorophyll contents. Dry weights of above ground plant tissue and roots in both plant seedlings were reduced by the elevated R irradiation. Cucumber seedlings treated with 2 and 8 $mol{\cdot}m^{-2}{\cdot}s^{-1}$ and tomato seedlings treated with 8 ${\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ had significantly lower T/R ratio the other treatments tested.

Criteria of Fruit Quality and Harvest for Production of High Quality Small and Medium-Sized Fruits in 'Hanareum' Pear (Pyrus pyrifolia Nakai) (고품질 중소과 생산을 위한 '한아름' 배의 과실 품질 및 수확 기준)

  • Park, YoSup;Kwon, YongHee
    • Journal of Bio-Environment Control
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    • v.23 no.2
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    • pp.131-138
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    • 2014
  • 'Hanareum' pear is expected to produce the high quality small and medium-sized fruits without any major changes in the current cultivation techniques, inasmuch as its basic characteristics are already included in the range of small and medium-sized fruits. Thus, we tested the possibility of utilization of 'Hanareum' pear for producing small and medium-sized fruits, while establishing the limit of minimum fruit weight for the fruits in high quality. With the results of correlation analysis, it was possible to predict the fruit weight through the observation of soluble solid contents and flesh firmness, because both factors were closely correlated to fruit weight in all treatments. Moreover, these factors were confirmed to be useful indicators of forecasting consumer preference in the sensory evaluation. The fruit marketability was excellent under the conditions that were greater than $11.6^{\circ}Bx$ and less than 25.6 N for soluble solid contents and flesh firmness, respectively. When applying these standards of fruit quality to the results of regression analysis for fruit weight, non-treated fruits fulfilled both standards when the fruit weight was higher than 436 g, and the quality uniformity was also high on this state. Therefore, the production of high quality small and medium-sized fruits was determined to be under this condition. The weight limit for GA treated fruits was 620 g, and both fruit quality and uniformity were below the weight range of small and medium-sized fruits, with 300~500 g. Thus, GA treatment was suggested to be avoided, in order to produce the high quality small and medium-sized fruits.

Physiological Responses for Soil Water Stresses in 'Mihong' Peach Tree (복숭아 '미홍'의 토양 수분 스트레스에 따른 생리반응)

  • Kwon, Yong Hee;Lee, Jae Man;Han, Hyun Hee;Ryu, Suhyun;Jeong, Jae Hoon;Do, Gyung-Ran;Han, Jeom Hwa;Lee, Han-Chan;Park, Hee-Seung
    • Journal of Bio-Environment Control
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    • v.25 no.4
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    • pp.255-261
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    • 2016
  • The present study was conducted to characterize physiological responses of aerial part according to soil water stresses in 'Mihong' peach trees. Discolorations, wilting and falling of leaves were observed in chronological order as response for waterlogging and no irrigation and the phenomena started from basal to end of shoots. Shoot growth in elongation and thickness decreased and fallen leaves were severe in waterlogged trees. Function of water uptake by roots and photosynthesis and leaf respiration decreased by waterlogging. Leaf chlorophyll contents decreased in both treatments. In waterlogging treatment, decrease of chlorophyll was observed in normal leaves with waterlogging using light microscopy. Starch content was lower in both treatment and carbohydrate content was lower in root with waterlogging. These results demonstrated that waterlogging weakened the function of soil water uptake and movement and decreased photosynthesis and fallen leaves. Finally the peach trees would wither or suffer low temperature damage through the shortage of reserve accumulations. We suggested that waterlogging damage in peach trees could be reduced to take notice of irrigation and install drainage facility to improve soil condition.

Biological Activities of Ethanol Extracts from Monascus-Fermented Chinese Yam (발효 홍국마 에탄올 추출물의 생리활성 효과)

  • Jeon, Chun-Pyo;Lee, Jung-Bok;Choi, Chung-Sig;Kwon, Gi-Seok
    • Journal of Life Science
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    • v.21 no.8
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    • pp.1142-1148
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    • 2011
  • This study was conducted to investigate antioxidative and physiological activities of ethanol extracts concentration from Monascus-Fermented Chinese Yam (MFCY). The ethanol extracts from MFCY were measured to examine pigment, monacolin K content, total polyphenol content, DPPH radical scavenging activity and angiotensin converting enzyme inhibitory effects. In this study, the results show that Monascus sp. MK2, with Dioscorea batatas Dence. as the substrate, can produce pigments (red, orange, and yellow), monacolin K content, total polyphenol content and DPPH radical scavenging activity at 13.48 (red), 11.66 (orange), 12.77 (yellow), 462.78 (mg/kg), 658.8 (mg/kg) and 92.8% in EtOH extract, respectively. In addition, ACE inhibitory activity was shown to be 74.55% in EtOH extract. Therefore, it can be concluded that Dioscorea batatas Dence is the best fermentative substrate for Monascus species to produce secondary metabolites as biomedicinal substances.

Syngeographical Characteristics of Forest Vegetation in Limestone Areas, Mt. Deokhang, Kangwondo (강원도 덕항산 석회암지대 산림식생의 군락지리 특성)

  • Bae, Kwan-Ho;Kim, Jun-Soo;Cho, Hyun-Je;Yun, Chung-Weon;Cho, Yong-Chan
    • Korean Journal of Environment and Ecology
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    • v.28 no.2
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    • pp.161-170
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    • 2014
  • This study classified the types of major forest vegetation and analyzed the syngeographical characteristics of the vegetation in the Mt. Deokhang with exhumed lime rocks. The forest vegetation on the ridge of Mt. Deokhang was classified into 1 community group, 3 communities, 2 groups, and 2 subgroups, 5 types in total. The syngeographical form of Deokhangsan fully exhibited the topographical peculiarity, proximity to subalpine, and limestone zone. In addition, Carex ciliato-marginata, Carex humilis var. nana, and so forth which appear as species with high consistency in the well-drained semiarid zone represent the soil environment of the lime stone area and northern plants such as Carex ussuriensis and Asplenium ruta-muraria represent the characteristics of the vegetation in alpestrine in close proximity. Moreover, the Carpinus turczaninowii in the area appears to show the characteristics of migratory vegetation due to the peculiarity of the habitat. The syngeographical characteristics of this study area show both the characteristics of the habitat and phytogeographic characteristics. Furthermore, they are expected to contribute to the diversity in community and habitat form in Korean peninsula in terms of vegetation science.

Anthocyanin Composition and Characteristics of 'Gaeryangmeoru', 'Kyoho', and 'Hongisul' Grape Varieties ('개량머루', '거봉' 및 '홍이슬' 품종의 anthocyanin 조성과 특징)

  • Kwon, YongHee;Park, Hee-Seung
    • Horticultural Science & Technology
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    • v.33 no.4
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    • pp.470-478
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    • 2015
  • This study was investigated the anthocyanin composition of 'Gaeryangmeoru', 'Kyoho', and 'Hongisul' grapes cultivated in Korea using ultra-performance liquid chromatography (UPLC) coupled to a mass spectrometer (MS) equipped with an ESI (electrospray ionization) source. 'Gaeryangmeoru' is a dark-blue grape used for winemaking that can reach its coloring in unfavorable weather. The 'Kyoho' and 'Hongisul' varieties are hybrid grapes that feature black and pink skin, respectively. The anthocyanins extracted from the peels of grapes were analyzed using UPLC-ESI-MS/MS. Twenty-five anthocyanins were identified in the 'Gaeryangmeoru' and 'Kyoho' varieties, and 21 were identified in the 'Hongisul' variety. Eight, 14 and five predominant anthocyanins were identified in 'Gaeryangmeoru', 'Kyoho' and 'Hongisul' grape respectively. In all three varieties, mono-glucosides were 2.3-5.9 times more abundant than di-glucoside. Malvidin was the predominant anthocyanidin in 'Gaeryangmeoru' (44.1%) and 'Kyoho' (56.5%), but cyanidin (96.8%) was in 'Hongisul'. The acylated anthocyanins in 'Gaeryangmeoru' (2.0%) were rare, whereas acylated anthocyanins with p-coumaric acid were predominant in 'Kyoho' (40.9%) and 'Hongisul' (70.7%). In particular, cyanidin feruloyl glucoside was found only in the 'Hongisul' cultivar and considered to be useful as a criterion for identification of the variety. As a result, the grape varieties were demonstrated to have variety-specific anthocyanin characteristics, enabling classification based on anthocyanin composition in terms of anthocyanidins, glucosylation and acylation. The taxonomical application of anthocyanin composition confirmed the possibility that 'Gaeryangmeoru' originated from Vitis amurensis or its hybrids, and the 'Hongisul' grape was distinguished from other grapes by cyanidin feruloyl glucoside.