• 한국미생물·생명공학회지
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• 제32권4호
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• pp.291-296
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• 2004

고품질, 기능성 청국장 제조를 위한 균주 개발 연구의 일환으로, 재래식 청국장으로부터 생육이 빠르며, 면역증강활성이 우수한 JB-1 균주는 Bacillus pumilus 로 확인되었으며 Bacillus pumilus KCTC 10461BP로 균주기탁 되었다. JB-1으로 발효시킨 청국장은 관능성과 영양성이 우수하며 물추출물 2mg/ml의 농도에서 410% 정도의 면역세포 증식율을 나타내었으며, 산업적 규모의 6 kg 의 대두증자에 20 ml 배양액을 접종함으로서 16시간 이내에 저이취와 높은 생균수 및 아미노산 함량의 청국장 제조가 가능함을 확인하였다.

• 농약과학회지
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• 제11권1호
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• pp.46-51
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• 2007

본 연구에서는 복숭아순나방 (Grapholita molesta) 수컷 유인력 교란이라는 측면에서 기존에 주로 사용되던 $Isomate^{(R)}$-M ROSSO를 다른 형태의 방출기인 $SPLAT^{(R)}$과 야외 적용 실험을 통해 비교하였다. 이를 위해 기존 방출기인 Isomate의 과거 3년간 교미교란 효과를 분석하였고, 이후 두 가지 방출기의 비교는 여러지역에서 지역내 반복을 설정하면서 동일 기간에 이뤄졌다. 무처리구에서 잡힌 수컷 밀도를 기준으로 복숭아순나방은 야외에서 4회 성충 발생기를 보이며, 월동세대의 밀도가 높음에 따라 이후 이어지는 생식세대의 크기가 높아진다는 정 상관관계를 보였다. Isomate 방출기는 지난 3년간 포장에서 복숭아순나방 수컷 교미행동을 유의성있게 교란한 것으로 나타났다. 두 페로몬 방출기를 비교할 경우, SPLAT이 보다 우수한 교란효과를 나타냈다. 이러한 수컷 유인력에 있어서 차이점은 이 두 방출기에 포함된 주요 페로몬 화학성분비의 차이에서 기인된 것으로 여겨진다.

• 한국버섯학회지
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• 제18권1호
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• pp.29-36
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• 2020

P. linteus(품종명: 고려상황, PLKS)와 P. baumii (장수상황, PBJS)를 동일한 조건하에서 인공재배하여 얻어진 자실체를 70% 메탄올, 60% 에탄올, 열수추출하여 폴리페놀과 플라보노이드 측정 시 함량은 60%에탄올 추출물에서 가장 높게 나타났다. 60%에탄올을 용매로 PLKS와 PBJS의 자실체를 추출하여 농도별로 DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2'-azino-bis (3-ethylbisthiazoline-6-sulfonic acid) 라디칼 소거능을 측정한 결과 PLKS와 PBJS는 거의 유사한 항산화 활성을 보였으며 자실체가 균사체보다 2-3배 이상 높은 항산화 활성이 측정되었으나 FRAP (ferric reducing antioxidant power)분석에서는 자실체와 균사체 간에 큰 차이가 없는 것을 확인하였다. PLKS와 PBJS의 자실체 ethyl acetate 분획물을 HPLC 분석한 결과 styrylpyrone 계 폴리페놀 성분인 davallialactone, hispidin, hypholomine B, inoscavin A를 검출할 수 있었으나 균사체에서는 검출되지 않아 자실체 특이적인 화합물인 것을 확인하였다.

• The Plant Pathology Journal
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• 제32권4호
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• pp.311-320
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• 2016

Xanthomonas axonopodis pv. glycines (Xag) is a necrotrophic bacterial pathogen of the soybean that causes bacterial pustules and is a nonhost pathogen of the chili pepper. In the current study, chili pepper fruit wound inoculated in planta with Xag 8ra formed necrotic lesions on the fruit surface and induced several structural and chemical barriers systemically in the fruit tissue. The initial defense response included programmed cell death of necrotizing and necrotized cells, which was characterized by nuclear DNA cleavage, as detected by TUNEL-confocal laser scanning microscopy (CLSM), and phosphatidylserine exposure on cell walls distal to the infection site, as detected by Annexin V FLUOS-CLSM. These two responses may facilitate cell killing and enhance transportation of cell wall materials used for cell wall thickening, respectively. The cells beneath the necrotic tissue were enlarged and divided to form periclinal cell walls, resulting in extensive formation of several parallel boundary layers at the later stages of infection, accompanying the deposition of wall fortification materials for strengthening structural defenses. These results suggest that nonhost resistance of chili pepper fruit against the nonhost necrotrophic pathogen Xag 8ra is activated systematically from the initial infection until termination of the infection cycle, resulting in complete inhibition of bacterial pathogenesis by utilizing organspecific in situ physiological events governed by the expression of genes in the plant fruit organ.

• 한국토양비료학회지
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• 제42권2호
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• pp.139-143
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• 2009

인지질 지방산을 분석하여 특정 미생물군의 수직적 분포와 토층간 미생물 군집 패턴을 조사하였다. 경북 농업기술원에 위치하고, 질소, 인산, 가리의 화학비료만 장기 연용한 논과 밭 포장에서 15 cm 깊이까지 토양을 채취하였다. 인지질 지표 지방산을 주요인 분석으로 분석하여 토양 미생물 군집을 분석한 결과 논과 밭 토양의 미생물 군집은 뚜렷하게 구분되었으며, 토층간 차이보다 논과 밭의 차이가 더 컸다. 논보다 밭은 토층이 깊어짐에 따라 미생물 군집이 급격하게 변하였는데, 미생물 군집 측면에서 밭보다 논의 표층이 더 두껍다고 볼 수 있다. cyclopropyl/monoenoic precursor 비율과 전체 포화지방산/전체 불포화 지방산 비율은 토심이 깊어짐에 따라 증가하였는데, 이는 토심이 깊어질수록 탄소원과 통기가 부족하기 때문에 일어나는 현상으로 보인다. 대체로 표토는 그램음성균, 곰팡이 등의 상대적 비율이 높고 토심이 깊어질수록 세균과 방선균의 상대적 비율이 높아졌다.

• Animal Systematics, Evolution and Diversity
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• 제29권2호
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• pp.136-143
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• 2013

The collared scops owl that occurs in Korea is a protected species but its exact specific status has been questioned. To resolve the species status, a molecular phylogenetic analysis was conducted using two fragments of mitochondrial DNA, cytochrome b (cyt b, 891 bp) and NADH dehydrogenase subunit 2 (ND2, 627 bp) genes. Phylogenetic trees of cyt b revealed that all Korean specimens formed a monophyletic group with Japanese scops owl Otus semitorques with very low sequence divergence (d=0.008). We obtained a similar ND2 tree as well (d=0.003); however, the genetic distance between Korean individuals and O. lempiji from GenBank (AJ004026-7, EU348987, and EU601036) was very high and sufficient enough to separate them as species (cyt b, d=0.118; ND2, d=0.113). We also found that Korean species showed high differentiation from O. bakkamoena (AJ004018-20 and EU601034; cyt b, d=0.106; ND2, d=0.113) and O. lettia (EU601109 and EU601033, cyt b, d=0.110; ND2, d=0.117) as well. Therefore, we suggest that the Korean collared scops owl should be designated as Otus semitorques.

• 한국물환경학회지
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• 제20권5호
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• pp.422-431
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• 2004

Water quality modeling was performed for the purpose of diagnosis and prediction of water quality in Gyoung Choen reservoir, using EUTR05/WASP Build model. WASP Builder is capable of visual display in window and it has an advantage of updating and modification for data. Field data of 1992, Spring, Summer, and Fall, were used to calibrate model and these results were validated using data of 2000, Spring, Summer, and Fall. The reservoir was divided into 4 epilimnion segments. Water quality system for modeling were consist of BOD, Chlorophyll-a, DO, $NH_3-N$, $NO_3-N$, T-N, $PO_4-P$, T-P. The results of water quality modelling using EUTR05/WASP Builder, a range of the Correlation for calibration of BOD, T-N, T-P, and Chlorophyll-a according to three seasons are 0.63~0.90, 0.81~0.97, 0.75~0.98, and 0.77~0.98 respectively. And the correlation between simulated and observed values for verification of BOD, T-N, T-P, and Chlorophyll-a according to three seasons are 0.93, 0.94, 0.81, and 0.80 respectively. Among the pollutant sources for a basin of the Gyoung Choen reservoir, generated amount of livestock is the highest and BOD, T-N, T-P of generated loading percentage are 94%, 81%, and 95%. So, we suppose that inflow load amount will decrease 50% and increase 50% only livestock about current load amount. If increasing load amount of livestock 50% in segment 2 and 3, BOD, T-N, and T-P simulated increasing to range of $0.02~0.15mg/{\ell}$, $0.029~0.08mg/{\ell}$, $0.011~0.029mg/{\ell}$ in comparison with current water quality

• 한국농공학회논문집
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• 제46권7호
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• pp.35-44
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• 2004

In this study, a ventilation model was developed to determine a ventilation rate for the balance of heat, moisture and $CO_{2}$ in a mushroom house. Internal and external temperature, relative humidity and $CO_{2}$ concentration were measured and used to validate the ventilation model. The effects of various environmental factors on physiological responses of mushroom were also investigated. The verified model was simulated under the observed ventilation rates with a difference of$ 0.001{\~}0.065\;m^{3}{\cdot}S^{-1}$ (relative error of $0.3{\~}18.9\%$) when external temperature varied 22.5 to $24.8^{circ}C$ and average ventilation rates was $0.35m^{3}{\cdot}S^{-1}$. The optimal conditions for mushroom growth (internal temperature $22 ^{circ}C$, relative humidity $80\%$, $CO_{2}$ concentration 1,000 ppm) were used for the model application with external temperature, relative humidity and $CO_{2}$ concentration of $27.5{\~}33.5^{circ}C$, $60\%$, and 355 ppm, respectively. Thermal balance was a important factor for an optimum ventilation up to the external temperature of $32^{circ}C$, while $CO_{2}$ concentration balance was more important over $32^{circ}C$. This suggests that humidification for moisture balance is required to maintain temperature and $CO_{2}$ concentration at an optimal level by ventilation in a mushroom house.

• 수산해양기술연구
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• 제51권2호
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• pp.195-202
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• 2015

Hundreds of Steller sea lions, Eumetopias jubatus, migrate from Sakhalin and the northern Kuril Islands to Hokkaido in Japan every winter. During this migration, they may use their roaring sounds to navigate and to maintain their groups. We recorded the roars of wild Steller sea lions that had landed on reefs on the west coast of Hokkaido, and those of captive sea lions, while making video recordings. The fundamental frequency ($F_0$), Formant frequency ($F_1$), pulse repetition rate (PRR), and duration of syllables (T) were analyzed using a sonagraph. $F_0$, $F_1$, and PRR of the roars emitted by captive sea lions increased in the order male, female. By contrast, the $F_1$ of wild males was lower than that of females, while the $F_0$ and PRR of wild males and females did not differ statistically. These results suggest regional differences between the five groups showed that.

• 원예과학기술지
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• 제32권3호
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• pp.382-389
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• 2014

Raphanus sativus L. cv. Taebaek, a efficiently microspore-derived embryo (MDE)-forming cultivar, and 'Chungwoon', a non-MDE-forming cultivar were selected as donor plants for isolated microspore culture. Radish flower bud of 2.0 (small, S), 4.0 (medium, M), and 6.0 (large, L) ${\pm}$ 0.5 mm in length were isolated to determine the temporal relationship between flower bud size and MED yield. Anatomical observations revealed no difference in the structure of the flower buds between the two cultivars. In both cultivars, the stigmas were much longer than the floral leaf in M-sized flower buds. The MDE yields for 'Taebaek' per petri dish were 6.6 and 1.3 for M- and L-sized of flower buds, respectively, but MDE formation was not induced in the S flower buds. On the other hand, 'Chungwoon' failed to form MDEs in all flower buds. The microspore density of 'Taebaek' was 1.3 times more than that of 'Chungwoon' for M sized flower buds. Of the M-sized buds from 'Taebaek' and 'Chungwoon', 92.1 and 81.6%, respectively, were in the late uninucleate microspore stage, which is characterized by the highest frequency of MDE formation. Anatomical observations of MDE formation revealed that the microspores were able to divide to form a primordium from which cell division took place continuously in the 'Teabeak' cultivar. However, the microspores of 'Chungwoon' failed to progress beyond the primodium stage, resulting in lack of MDE formation. By contrast, after the formation of the primordium, various developmental stages of embyos from microspore were observed in the 'Taebaek' cultivar. These results can be used to determine MDE forming potentials of radish cultivars.