• Title/Summary/Keyword: bioreactor process

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Study on Determination of Design Factor of Bioreactor for Sulfate Reduction in Mine Drainage (광산배수 내 황산염 저감을 위한 생물반응기의 설계인자 도출 연구)

  • Kim, Kang-Ho;Kang, Chan-Ung;Kim, Sun-Joon;Kim, Tae-Heok;Ji, Won-Hyun;Jang, Hang-Seok;Park, Hyun-Sung
    • Journal of the Korean Society of Mineral and Energy Resources Engineers
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    • v.55 no.6
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    • pp.527-537
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    • 2018
  • Column tests of a sulfate reducing bacteria (SRB) bioreactor were conducted to determine the design factors for sulfate-rich mine drainage. Various substrates were applied to the bioreactor, including cow manure and its mixture with a mushroom compost, with rice straw and limestone as subsidiary materials. This procedure provided a removal efficiency of up to 82% of the total sulfur with the mixture of cow manure (70%), mushroom compost (10%) and rice straw (20%), and higher efficiencies were observed after 2 days of retention time. In the downflow condition of the flow direction, oxygen supply and re-oxidation of the sulfates occurred, causing a decrease in sulfate removal efficiency. The addition of an inorganic sludge containing heavy metals, which was intended for production of metal-sulfides in the bioreactor, had a negative effect on the long-term operation owing to arsenic release and toxicity to the SRB. The results thus show that a bioreactor using a mixed substrate with cow manure and operating in the downflow direction could reduce sulfates and total dissolved sulfur content; this process confirms the applicability of the SRB bioreactor to sulfate-rich saline drainage.

Flux Optimization Using Genetic Algorithms in Membrane Bioreactor

  • Kim Jung-Mo;Park Chul-Hwan;Kim Seung-Wook;Kim Sang-Yong
    • Journal of Microbiology and Biotechnology
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    • v.16 no.6
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    • pp.863-869
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    • 2006
  • The behavior of submerged membrane bioreactor (SMBR) filtration systems utilizing rapid air backpulsing as a cleaning technique to remove reversible foulants was investigated using a genetic algorithm (GA). A customized genetic algorithm with suitable genetic operators was used to generate optimal time profiles. From experiments utilizing short and long periods of forward and reverse filtration, various experimental process parameters were determined. The GA indicated that the optimal values for the net flux fell between 263-270 LMH when the forward filtration time ($t_f$) was 30-37 s and the backward filtration time ($t_b$) was 0.19-0.27 s. The experimental data confirmed the optimal backpulse duration and frequency that maximized the net flux, which represented a four-fold improvement in 24-h backpulsing experiments compared with the absence of backpulsing. Consequently, the identification of a region of feasible parameters and nonlinear flux optimization were both successfully performed by the genetic algorithm, meaning the genetic algorithm-based optimization proved to be useful for solving SMBR flux optimization problems.

Evaluation of Operation Characteristics with Aeration Time in Intermittent Aeration Membrane Bioreactor (간헐포기 MBR공정에서 포기시간에 따른 운전특성 평가)

  • Lim, Bong-Su;Choi, Bong-Cheol
    • Journal of Korean Society on Water Environment
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    • v.21 no.4
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    • pp.353-359
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    • 2005
  • This study was conducted to evaluate the operation characteristics with aeration time in intermittent aeration membrane bioreactor. The BOD removal efficiency rate of this process was over than 97% regardless of aeration on/off time. To get over than 82% of nitrogen removal efficiency rate, aeration off time needs more than 70 minutes in reactor. Specific denitrfication rate was 2.68 mg $NO_3-N/gMv/hr$ in 40/80 min aeration on/off time, was 2.6 times more than 60/60 min, and 1.4 times more than 50/70 min in 6,300 mg/L of MLSS concentration. Specific nitrification rate was 1.96 mg $NH_4-N/gMv/hr$ in 50/70 min, was 1.4 times more than 40/80 min, but it was effectded little upon nitrification. Microbial activity was effected little according to aeration on/off time, oxygen demend was reduced according to aeration off time increased and microbial concentration increased. The longer aeration off time become, the higher Extraceller Pollymeric Substance (EPS), 50/70 min and 40/80 min in aeration on/off time was increased 1.6 times and 2.7 times, respectively more than 60/60 min because of increase of operation pressure.

RESEARCH PAPERS : THE KINETICS ON THE BIOLOGICAL REACTION IN MEMBRANE BIOREACTOR (MBR) WITH GRAVITATIONAL AND TRANSVERSAL FILTRATION

  • Jang, Nam-J.;Hwang, Moon-H.;Yeo, Young-H.;Shim, Wang-G.;S. Vigneswaran;Kim, In-S.
    • Environmental Engineering Research
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    • v.9 no.5
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    • pp.238-247
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    • 2004
  • The objective of this study was to develop kinetic model for the MBR and investigate kinetic characteristics of the gravitational flow transverse direction MBR system. Kinetic model was derived by mass balance of substratc and biomass combined with empirical membranc filtration rerm for the MBR. To find kinctic values, permeale flux and COD removal were analyzed through the laboratory, MBR operation as different solids retention times. Permeate flux was ranged 2.5-5.0 LMH (L/m$^2$/hr) as sludge characteristics in each run. Although the soluble COD in the bioreactor was changed, the effluent COD was stable as average 99% removal rate during the experimental periods. Y$_g$ of this MBR system was higher than those of cross-flow MBR processes. The kinetics of this MBR showed that smaller k, larger b, and larger K$_s$ values than the conventional activated sludge process. These results indicated that substrate was used for cell maintenance rather than growth in this MBR system.

Quorum quenching for effective control of biofouling in membrane bioreactor: A comprehensive review of approaches, applications, and challenges

  • Kose-Mutlu, Borte;Ergon-Can, Tulay;Koyuncu, Ismail;Lee, Chung-Hak
    • Environmental Engineering Research
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    • v.24 no.4
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    • pp.543-558
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    • 2019
  • In comparison to alternative advanced wastewater treatment technologies, the main problem associated with membrane bioreactor (MBR) technology, which has become prominent in recent years, is biofouling. Within these systems, biofouling is typically the result of a biofilm layer resulting from bacterial gathering. One biological system that can be employed to interrupt the process of bacterial gathering is called 'Quorum Quenching (QQ)'. Existing QQ applications can be classified using three main types: 1) bacterial/whole-cell applications, 2) direct enzyme applications, and 3) natural sourced compounds. The most common and widely recognized applications for membrane fouling control during MBR operation are bacterial and direct enzyme applications. The purpose of this review was to identify and assess biofilm formation mechanism and results, the suggestion of the QQ concept and its potential to control biofilm formation, and the means by which these QQ applications can be applied within the MBR and present QQ MBR studies.

A Study on Removal of Disinfection By-products in High Concentration Powdered Activated Carbon Membrane Bio-reactor Process for Advanced Water Treatment (고도정수처리를 위한 HCPAC-MBR 공정에서의 소독부산물 저감에 관한 연구)

  • Lee, Song-Hee;Jang, Sung-Woo;Seo, Gyu-Tae
    • Journal of Korean Society of Water and Wastewater
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    • v.20 no.1
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    • pp.27-34
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    • 2006
  • This study was conducted to evaluate the performance of a membrane bioreactor filled with high concentration of powdered activated carbon (HCPAC-MBR) to reduce DBPs at the drinking water treatment. The pilot system was installed after the rapid sand filtration process whose plant was the conventional treatment process. The removal efficiencies of DBPs were measured during pilot operation period of 2 years. HAA and THM removal rates could be maintained around 80~90% without any troubles and then tremendous reduction of HAA and THM reactivity were observed more than 52%. The average removal rate of HAA formation potential (FP) and THM formation potential (FP) were 70.5% and 67.6% respectively. It is clear that the PAC membrane bioreactor is highly applicable for advanced water treatment to control DBPs.

Biological Removal of EG from Weight Loss Treatment Wastewater & Complex Dyeing Process Wastewater

  • Lee, Hyeon-Uk;Im, Dong-Jun
    • 한국생물공학회:학술대회논문집
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    • 2001.11a
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    • pp.505-508
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    • 2001
  • An microorganism able to degrade ethylene glycol(EG) was developed. Using this microorganism, biological treatment of ethylene glycol was studied in Erlenmeyer flasks and a laboratory scale stirred loop bioreactor. The removal efficiencies of ethylene glycol from synthetic wastewater were 91.6% ${\sim}$ 97.7% at $30^{\circ}C$ ${\sim}$ $40^{\circ}C$, and 96.3% ${\sim}$ 97.9% at initial pH 9 ${\sim}$ 11 respectively. Also the removal efficiencies of ethylene glycol were found to be more then 92% at initial ethylene glycol concentration of 300mg/L ${\sim}$ I400mg/L. In treatment of weight loss treatment wastewater using Erlenmeyer flasks, the removal efficiencies of ethylene glycol were 79.6%. 82.5%. 77.6%. and 71.3% at initial pH 9. 10. 11. and 12.4 after 11 days of reaction. Moreover in treatment of complex dyeing process wastewater. the residual ethylene glycol was not detected at the initial pH 10.0 and pH 11.3 after 4 days of reaction. When stirred loop bioreactor was used for removing ethylene glycol, the residual ethylene glycol was not detected after 108 hrs and 60 hrs of reaction in batch treatment of weight loss treatment wastewater and complex dyeing process wastewater.

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Optimization of membrane fouling process for mustard tuber wastewater treatment in an anoxic-oxic biofilm-membrane bioreactor

  • Chai, Hongxiang;Li, Liang;Wei, Yinghua;Zhou, Jian;Kang, Wei;Shao, Zhiyu;He, Qiang
    • Environmental Engineering Research
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    • v.21 no.2
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    • pp.196-202
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    • 2016
  • Membrane bioreactor (MBR) technology has previously been used by water industry to treat high salinity wastewater. In this study, an anoxic-oxic biofilm-membrane bioreactor (AOB-MBR) system has been developed to treat mustard tuber wastewater of 10% salinity (calculated as NaCl). To figure out the effects of operating conditions of the AOB-MBR on membrane fouling rate ($K_V$), response surface methodology was used to evaluate the interaction effect of the three key operational parameters, namely time interval for pump (t), aeration intensity ($U_{Gr}$) and transmembrane pressure (TMP). The optimal condition for lowest membrane fouling rate ($K_V$) was obtained: time interval was 4.0 min, aeration intensity was $14.6 m^3/(m^2{\cdot}h)$ and transmembrane pressure was 19.0 kPa. And under this condition, the treatment efficiency with different influent loads, i.e. 1.0, 1.9 and $3.3kgCODm^{-3}d^{-1}$ was researched. When the reactor influent load was less than $1.9kgCODm^{-3}d^{-1}$, the effluent could meet the third discharge standard of "Integrated Wastewater Discharge Standard". This study suggests that the model fitted by response surface methodology can predict accurately membrane fouling rate within the specified design space. And it is feasible to apply the AOB-MBR in the pickled mustard tuber factory, achieving satisfying effluent quality.

A Basic Study on the Anaerobic Wastewater Treatment using Nonwoven Fabric Filter Bioreactor (부직포 여과막 생물반응조의 혐기성 폐수처리에 관한 기초연구)

  • Kim, Taek-Su;Bae, Min-Su;Cho, Yun-Kyung;Cho, Kwang-Myeung
    • Journal of Korean Society on Water Environment
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    • v.21 no.5
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    • pp.464-469
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    • 2005
  • In the nonwoven fabric filter bioreactor (NFBR), both the construction and the operation costs could be saved because a high concentration of microorganism can be maintained in the reactor as in the membrane bioreactor. However, the NFBR process has been investigated only under aerobic and/or anoxic conditions, In this research, a basic anaerobic treatment experiment was performed at $35^{\circ}C$ by feeding an airtight NFBR with a concentrated synthetic organic wastewater. The organic loading rate (OLR) of the NFBR was increased stepwise from $0.25kg\;COD/m^3-day$ to $0.77kg\;COD/m^3-day$ by gradually decreasing the hydraulic retention time from 20 days to 13 days. The results of the research showed that the best COD removal efficiency achieved at the OLR of $0.67kg\;COD/m^3-day$ with a value of 99.3%. The methane content of the produced gas was highest with a value of 61.2% at the OLR of $0.33g\;COD/m^3-day$. The highest methane production rate was $0.89g\;COD/m^3-day$ at the same OLR. The operation was terminated at the OLR of $0.77kg\;COD/m^3-day$ because of the deterioration in COD removal efficiency, gas production rate, and the methane content of the gas. Further researches are recommended for the NFBR to be employed for anaerobic treatment of organic wastewaters.

Fuzzy Logic Control of Rotating Drum Bioreactor for Improved Production of Amylase and Protease Enzymes by Aspergillus oryzae in Solid-State Fermentation

  • Sukumprasertsri, Monton;Unrean, Pornkamol;Pimsamarn, Jindarat;Kitsubun, Panit;Tongta, Anan
    • Journal of Microbiology and Biotechnology
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    • v.23 no.3
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    • pp.335-342
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    • 2013
  • In this study, we compared the performance of two control systems, fuzzy logic control (FLC) and conventional control (CC). The control systems were applied for controlling temperature and substrate moisture content in a solidstate fermentation for the biosynthesis of amylase and protease enzymes by Aspergillus oryzae. The fermentation process was achieved in a 200 L rotating drum bioreactor. Three factors affecting temperature and moisture content in the solid-state fermentation were considered. They were inlet air velocity, speed of the rotating drum bioreactor, and spray water addition. The fuzzy logic control system was designed using four input variables: air velocity, substrate temperature, fermentation time, and rotation speed. The temperature was controlled by two variables, inlet air velocity and rotational speed of bioreactor, while the moisture content was controlled by spray water. Experimental results confirmed that the FLC system could effectively control the temperature and moisture content of substrate better than the CC system, resulting in an increased enzyme production by A. oryzae. Thus, the fuzzy logic control is a promising control system that can be applied for enhanced production of enzymes in solidstate fermentation.