• Title/Summary/Keyword: biomass saccharification

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Chemical Characterization of Industrial Hemp (Cannabis sativa) Biomass as Biorefinery Feedstock

  • Shin, Soo-Jeong;Han, Gyu-Seong;Choi, In-Gyu;Han, Sim-Hee
    • Korean Journal of Plant Resources
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    • v.21 no.3
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    • pp.222-225
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    • 2008
  • Chemical composition and enzymatic saccharification characteristics of hemp woody core were investigated by their chemical composition analysis and enzymatic saccharification with commercially available cellulases (Celluclast 1.5L and Novozym 342). Hemp woody core have higher xylan and lower lignin contents than its bast fiber. Based on hemicelluloses and lignin composition, hemp woody core is similar with hardwood biomass. However, cellulose was more easily converted to glucose than xylan to xylose and this trend was confirmed both hemp woody core and yellow poplar. Hemp woody core biomass shows higher saccharification than yellow poplar (hardwood biomass) based on cellulose and xylan hydrolysis. With easier enzymatic saccharification in cellulose and xylan, and similar chemical composition, hemp woody core have better biorefinery feedstock characteristics than hardwood biomass.

Enzymatic saccharification of autohydrolyzed industrial hemp (Cannabis sativa L.) lignocellulosic biomass (자기가수분해 처리가 산업용 대마 목부 바이오매스의 효소 당화에 미치는 영향)

  • Shin, Soo-Jeong;Yu, Ju-Hyun;Lee, Soo-Min;Cho, Nam-Seok
    • 한국신재생에너지학회:학술대회논문집
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    • 2008.10a
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    • pp.74-76
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    • 2008
  • Autohydrolysis at different temperature levels was applied as industrial hemp pretreatment technique for glucose generation. Main structural components removed by autohydrolysis was xylan, which is more sensitive in acidic hydrolysis condition than cellulose or lignin. Higher temperature reaction conditions promoted more biomass components (xylan) removal than lower temperature, which led to better respond to enzymatic saccharification of residual biomass after autohydrolysis. With $185^{\circ}C$ and 60 min, saccharification degree was 53.0% of cellulose in hemp woody core biomass.

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Enzymatic saccharification of rice straw, a lignocellulosic biomass by the extracellular enzyme from KMU001 (KMU001 조효소에 의한 목질계 바이오매스, 볏짚의 효소당화성에 관한 연구)

  • Kim, Yeong-Suk;Lee, Young-Min;Cha, Chang-Jun;Yoon, Jeoung-Jun
    • 한국신재생에너지학회:학술대회논문집
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    • 2008.05a
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    • pp.225-228
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    • 2008
  • This study shows that lignocellulosic biomass saccharification work has been carried out with rice-straw by the extracellular enzyme from KMU001, and the enzymes produced in 5%(w/v) wood biomass were characterized by protein and various enzyme activity measurements. Several cellulases such as Endoglucanase(EG), $\beta$-D-1,4-Glucosidase(BGL), Cellobiohydrolase(CBH), and $\beta$-D-1,4-Xylanase (BXL) were detected. Saccharification of rice-straw by the enzyme yielded about 233mg/g of glucose after 48hrs.

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Ethanol Production from Lignocellulosic Biomass by Simultaneous Saccharification and Fermentation Employing the Reuse of Yeast and Enzyme

  • KIM, JUN-SUK;KYUNG-KEUN OH;SEUNG-WOOK KIM;YONG-SEOB JEONG;SUK-IN HONG
    • Journal of Microbiology and Biotechnology
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    • v.9 no.3
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    • pp.297-302
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    • 1999
  • Simultaneous saccharification and fermentation (SSF) experiments were carried out with a lignocellulosic biomass. The effects of temperature on enzymatic saccharification and the ethanol fermentation were also investigated. The batch SSF process gave a final ethanol concentration of 10.44 g/l and equivalent glucose yield of 0.55 g/g, which was increased by 67% or higher over the saccharification at 42℃. The optimal operating condition was found to vary in several parameters, such as the transmembrane pressure, permeation rate, and separation coefficient, related to the SSF combined with membrane system (semi-batch system). When the fermentation was operated in a semi-batch mode, the efficiency of the enzymes and yeast lasted three times longer than in a batch mode.

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Bioethanol Production Using Lignocellulosic Biomass-review Part 2. Saccharification and fermentation of biomass for generating ethanol

  • Sheikh, Mominul Islam;Kim, Chul-Hwan;Yesmin, Shabina;Lee, Ji-Yong;Kim, Gyeong-Chul;Ahn, Byeong-Il;Kim, Sung-Ho;Park, Hyeon-Jin
    • Journal of Korea Technical Association of The Pulp and Paper Industry
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    • v.42 no.5
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    • pp.15-23
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    • 2010
  • Bio-ethanol is the most potential next generation automotive fuel for reducing both consumption of crude oil and environmental pollution from renewable resources such as wood, forest residuals, agricultural leftovers and urban wastes. Lignocellulosic based materials can be broken down into individual sugars. Therefore, saccharification is one of the important steps for producing sugars, such as 6-C glucose, galactose, mannose and 5-C xylose, mannose and rhamnose. These sugars can be further broken down and fermented into ethanol. The main objective of this research is to study the feasibility and optimize saccharification and fermentation process for the conversion of lignocellulosic biomass to low cost bioethanol.

Effect of torrefaction on enzymatic saccharification of lignocellulosic biomass (목질계 바이오매스의 효소당화에서 반탄화 전처리 영향)

  • Choi, Hyoyeon;Pak, Daewon
    • Journal of Energy Engineering
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    • v.24 no.3
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    • pp.1-5
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    • 2015
  • This study is to investigate the effect of torrefaction on enzymatic hydrolysis of lignocellulosic biomass for bio-ethanol production. As a pretreatment, the torrefaction of lignocellulosic biomass was conducted in temperature of $250{\sim}350^{\circ}C$ in the absence of oxygen. Tween-80, nonionic surfactant, was tested to enhance saccharification efficiency by coping with hydrophobicity resulted from torrefaction. As a result, the glucose production from enzymatic hydrolysis of biomass pretreated by torrefaction was greater than that obtained from the non-pretreated biomass. Sugar conversion was higher when the biomass was saccharified with addition of tween-80. It was found that torrefaction can be applied as a preptreatment for lignocellulosic biomass and tween-80 is needed to enhance its enzyme saccharification.

Cellulose Utilization and Protein Productivity of Some Cellulolytic Fungal Co-cultures

  • Eyini, M.;Babitha, S.;Lee, Min-Woong
    • Mycobiology
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    • v.30 no.3
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    • pp.166-169
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    • 2002
  • Protein productivity by the cellulolytic fungi, Trichoderma viride(MTCC 800), Chaetomium globosum and Aspergillus terreus was compared in co-culture and mixed culture fermentations of cashewnut bran. Co-cultures were more effective in substrate saccharification, which ranged between $85{\sim}88%$ compared to the $62{\sim}67%$ saccharification shown by the monocultures. Maximum saccharification was induced by T. viride and C. globosum co-culture resulting in the highest 34% release of reducing sugars. The maximum 16.4% biomass protein and the highest protein productivity(0.58%) were shown by T. viride and A. terreus co-culture. A. terreus performed better in co-culture in the presence of T. viride rather than with C. globosum. Among the cellulolytic enzymes, FPase(Filter Paper Cellulase) activity was significantly higher in all the co-cultures and in the mixed culture than in their respective monocultures. Mixed culture fermentation involving all the three fungi was not effective in increasing the per cent saccharification or the biomass protein content over the co-cultures.

Bioelectrochemical Detoxification of Phenolic Compounds during Enzymatic Pre-Treatment of Rice Straw

  • Kondaveeti, Sanath;Pagolu, Raviteja;Patel, Sanjay K.S.;Kumar, Ashok;Bisht, Aarti;Das, Devashish;Kalia, Vipin Chandra;Kim, In-Won;Lee, Jung-Kul
    • Journal of Microbiology and Biotechnology
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    • v.29 no.11
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    • pp.1760-1768
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    • 2019
  • The use of lignocellulosic biomass such as rice straw can help subsidize the cost of producing value-added chemicals. However, inhibitory compounds, such as phenolics, produced during the pre-treatment of biomass, hamper the saccharification process. Laccase and electrochemical stimuli are both well known to reduce phenolic compounds. Therefore, in this study, we implemented a bioelectrochemical detoxification system (BEDS), a consolidated electrochemical and enzymatic process involving laccase, to enhance the detoxification of phenolics, and thus achieve a higher saccharification efficiency. Saccharification of pretreated rice straw using BEDS at 1.5 V showed 90% phenolic reduction (Phr), thereby resulting in a maximum saccharification yield of 85%. In addition, the specific power consumption when using BEDS (2.2 W/Kg Phr) was noted to be 24% lower than by the electrochemical process alone (2.89 W/kg Phr). To the best of our knowledge, this is the first study to implement BEDS for reduction of phenolic compounds in pretreated biomass.

Enzymatic Saccharification of Salix viminalis cv. Q683 Biomass for Bioethanol Production

  • Kim, Hak-Gon;Song, Hyun-Jin;Jeong, Mi-Jin;Sim, Seon-Jeong;Park, Dong-Jin;Yang, Jae-Kyung;Yoo, Seok-Bong;Yeo, Jin-Ki;Karigar, Chandrakant S.;Choi, Myung-Suk
    • Journal of Forest and Environmental Science
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    • v.27 no.3
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    • pp.143-149
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    • 2011
  • The possibility of employing biomass of Salix viminalis cv. Q683 as a resource of bio-energy was evaluated. The chemical analysis of S. viminalis cv. Q683 leaf biomass showed components such as, extractives (2.57%), lignin (39.06%), hemicellulose (21.61%), and cellulose (37.83%), whereas, its stem was composed of extractives (1.67%), lignin (23.54%), hemicellulose (33.64%), and cellulose (42.03%). The biomass of S. viminalis cv. Q683 was saccharified using two enzymes celluclast and viscozyme. The saccharification of S. viminalis cv. Q683 biomass was influenced by enzymes and their strengths. The optimal enzyme combination was found to be celluclast (59 FPU/g substrate) and viscozyme (24 FBG/g substrate). On saccharification the glucose from leaf and stem biomass was 7.5g/L and 11.7g/L, respectively after 72 hr of enzyme treatment. The biomass and enzyme-treated biomass served as the feedstock for ethanol production by fermentation. The ethanol production from stem and leaf biomass was 5.8 g/L and 2.2 g/L respectively, while the fermentation of the enzymatic hydrolysates yielded 5 g/L to 8 g/L bioethanol in 72 hours.

Design of Pretreatment Process in Cellulosic Ethanol Production (목질계 셀룰로오스 에탄올 생산공정에서 전처리과정의 설계)

  • Kim, Hyungjin;Lee, Seung Bum
    • Applied Chemistry for Engineering
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    • v.26 no.4
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    • pp.511-514
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    • 2015
  • A pretreatment process of cellulose decomposition to a monosaccharide plays an important role in the cellulosic ethanol production using the lignocellulosic biomass. In this study, a cellulosic ethanol was produced by using acidic hydrolysis and enzymatic saccharification process from the lignocellulosic biomass such as rice straw, sawdust, copying paper and newspaper. Three different pretreatment processes were compared; the acidic hydrolysis ($100^{\circ}C$, 1 h) using 10~30 wt% of sulfuric acid, the enzymatic saccharification (30 min) using celluclast ($55^{\circ}C$, pH = 5.0), AMG ($60^{\circ}C$, pH = 4.5), and spirizyme ($60^{\circ}C$, pH = 4.2) and also the hybrid process (enzymatic saccharification after acidic hydrolysis). The yield of cellulosic ethanol conversion with those pretreatment processes were obtained as the following order : hybrid process > acidic hydrolysis > enzymatic saccharification. The optimum fermentation time was proven to be two days in this work. The yield of cellulosic ethanol conversion using celluclast after the acidic hydrolysis with 20 wt% sulfuric acid were obtained as the following order : sawdust > rice straw > copying paper > newspaper when conducting enzymatic saccharification.