• Title/Summary/Keyword: biology major

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Investigation of PCR-RFLPs within Major Histocompatibility Complex B-G Genes Using Two Restriction Enzymes in Eight Breeds of Chinese Indigenous Chickens

  • Xu, R.F.;Li, K.;Chen, G.H.;Qiang, B.Y.Z.;Mo, D.L.;Fan, B.;Li, C.C.;Yu, M.;Zhu, M.J.;Xiong, T.A.;Liu, Bang
    • Asian-Australasian Journal of Animal Sciences
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    • v.18 no.7
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    • pp.942-948
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    • 2005

  • New polymorphism of major histocompatibility complex B-G genes was investigated by amplification and digestion of a 401bp fragment including intron 1 and exon 2 using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique with two restriction enzymes of Msp I and Tas I in eight breeds of Chinese indigenous chickens and one exotic breed. In the fragment region of the gene, three novel single nucleotide polymorphisms (SNPs) were detected at the two restriction sites. We found the transition of two nucleotides of A294G and T295C occurred at Tas I restriction site, and consequently led to a non-synonymous substitution of asparagine into serine at position 54 within the deduced amino acid sequence of immunoglobulin variable-region-like domain encoded by the exon 2 of B-G gene. It was observed at rare frequency that a single mutation of A294G occurring at the site, also caused an identical substitution of amino acid, asparagine 54-to-serine, to that we described previously. And the transversion of G319C at Msp I site led to a non-synonymous substitution, glutamine 62-to-histidine. The new alleles and allele frequencies identified by the PCR-RFLP method with the two enzymes were characterized, of which the allele A and B frequencies at Msp I and Tas I loci were given disequilibrium distribution either in the eight Chinese local breeds or in the exotic breed. By comparison, allele A at Msp I locus tended to be dominant, while, the allele B at Tas I locus tended to be dominant in all of the breeds analyzed. In Tibetan chickens, the preliminary association analysis revealed that no significant difference was observed between the different genotypes identified at the Msp I and Tas I loci and the laying performance traits, respectively.

  • https://doi.org/10.5713/ajas.2005.942 인용 PDF KSCI

A New Species and Three New Records of the Families Gnaphosidae and Clubionidae (Araneae) from Korea

  • Seo, Bo Keun
    • Korean Journal of Environmental Biology
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    • v.35 no.2
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    • pp.191-197
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    • 2017

  • Four species of the families Gnaphosidae and Clubionidae are reported. Cladothela unmunensis n. sp. is described from Gyeongsangbuk-do, Korea. Drassodes longispinus Marusik and Logunov, 1995, Gnaphosa inconspecta Simon, 1878 and Clubiona tongdaoensis Zhang, Yin, Bao and Kim, 1997 are newly recorded to the Korean spider fauna.

  • https://doi.org/10.11626/KJEB.2017.35.2.191 인용 PDF KSCI

Four New Species of the Genera Trilacuna, Orchestina and Hahnia of the Families Oonopidae and Hahniidae (Araneae) from Korea

  • Seo, Bo Keun
    • Korean Journal of Environmental Biology
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    • v.35 no.2
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    • pp.184-190
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    • 2017

  • Four new species of the spider genera Trilacuna Tong and Li, 2007, Orchestina Simon, 1882 and Hahnia C. L. Koch, 1841 are described from Korea; Trilacuna hansanensis n. sp., Orchestina infirma n. sp., Hahnia montana n. sp. and Hahnia implexa n. sp. The genera Trilacuna and Orchestina are reported from Korea for the first time.

  • https://doi.org/10.11626/KJEB.2017.35.2.184 인용 PDF KSCI

Multiple Antibiotic Resistance in Pseudomonas putida Associated with Overproduction of a Membrane Protein

  • JUNG NAM KIM;HO GUN RHIE
    • Proceedings of the Korea Society of Environmental Toocicology Conference
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    • 2001.05a
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    • pp.140-140
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    • 2001

  • Porins are major outer membrane proteins which produce non-specific aqueous channels across the membrane that permit the diffusion into the bacterial cells of hydrophilic compounds including sugars, amino acids, and antibiotics. In some gram-negative organisms, antibiotic resistance can be induced by mutational loss of channel that causes a decrease in outer membrane permeability. (omitted)

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