• Journal of the Korean Society of Food Science and Nutrition
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• v.44 no.8
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• pp.1172-1179
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• 2015

This study investigated optimal extraction conditions for application of Ulmus pumila L. as a natural antioxidant. U. pumila L. was extracted using ethanol (EtOH) at various concentrations (0, 40, and 80%) and extraction times (1, 2, and 3 h) at $70^{\circ}C$ and then evaluated for extraction yield, total phenolic contents, total flavonoid contents, as well as antioxidant activities [2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity, reducing power, and oxygen radical absorbing capacity (ORAC)]. Antioxidant activities were correlated with total phenolic and flavonoid contents. Of the solvent conditions, 80% EtOH extracts for 3 h at $70^{\circ}C$ showed the highest total phenolic and flavonoid contents with strong antioxidant activities, although there were no significant time effects on DPPH and ABTS radical scavenging activities and reducing power. However, ORAC values of all EtOH extracts remarkably increased in a time-dependent manner. In addition, 80% EtOH extract for 3 h exhibited strong antioxidant effects on HDF and 3T3-L1 cells. Therefore, the antioxidant capacity of U. pumila L., may due to phenolic and flavonoid contents, and extraction conditions were 80% EtOH for 3 h at $70^{\circ}C$. This extract could be a good source for natural antioxidants.

• Microbiology and Biotechnology Letters
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• v.39 no.1
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• pp.63-69
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• 2011

In this study, the antibacterial activity and the antioxidative effects, inhibitory effects on tyrosinase of Lespedeza cuneata G. Don extracts were investigated. MIC value of ethyl acetate fraction from L. cuneata G. Don on P. ovale (0.125%) showed that the antibacterial activity of the ethyl acetate fraction was higher than methyl paraben. The aglycone fraction of L. cuneata G. Don (14.63 ${\mu}g$/mL) showed the most prominent the free radical (1,1-diphenyl-2-picryl-hydrazyl, DPPH) scavenging activity ($FSC_{50}$). Reactive oxygen species (ROS) scavenging activities ($OSC_{50}$) of L. cuneata G. Don fraction on $Fe^{3+}$-EDTA/$H_2O_2$ system were investigated using the luminol-dependent chemiluminescence assay. The aglycone fraction of L. cuneata G. Don (0.07 ${\mu}g$/mL) showed the most prominent ROS scavenging activity. The protective effects of extract/fractions of L. cuneata G. Don on the rose-bengal sensitized photohemolysis of human erythrocytes were investigated. The L. cuneata G. Don extracts suppressed photohemolysis in a concentration dependent manner (1 ~ 50 ${\mu}g$/mL). The inhibitory effects ($IC_{50}$) of L. cuneata G. Don extracts on tyrosinase were determined with ethyl acetate fraction (104.83 ${\mu}g$/mL) and aglycone fraction (27.55 ${\mu}g$/mL) of L. cuneata G. Don extract. These results indicate that L. cuneata G. Don extract/fractions can function as high potential as bactericide against the pathogenic bacteria and antioxidant in biological systems, particularly skin exposed to UV radiation by scavenging $^1O_2$ and other ROS, and protect cellular membranes against ROS. Extract/fractions of L. cuneata G. Don could be applicable to new functional cosmetics for antiaging, antioxidant, and antibacterial activity.

• Korean Journal of Environment and Ecology
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• v.20 no.3
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• pp.289-298
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• 2006

The purpose of this study was to investigate salt marsh flora and vegetation in the mouth of Mankyeong river estuary area where has a project for Sea Man Geum Reclaimed Land so that we can foster a foundation on restoration of an ecological habitat, development of applicable plants and establishment of a conservation policy after developing the reclaimed land for salt marsh vegetation which has great ecological value. As a result of this research, there are 10 families 25 genera 29 species and 3 varieties of vascular plants in the Mankyong-river estuary area. These are 0.76% among 4,191 of Korean vascular plants. There are also 5 families 6 genera 6 species and 1 varietiy of the naturalized plants which are 7 taxa in total and 3.85% of indicators of naturalized plants. Firstly, a district of low tide marsh has below 5% of vegetation coverage of Suaeda japonica and the vegetation cover was increasing rapidly while moving to a place of high tide marsh which is in the direction to a bank. In general, a range of from low tide marsh to high tide marsh is distributed with sequence of Suaeda japonica$\rightarrow$Suaeda maritima$\rightarrow$Suaeda japonica$\rightarrow$Aster tripolium$\rightarrow$Artemisia scoparia$\rightarrow$Carex scabrifolia$\rightarrow$Zoysia sinica$\rightarrow$Phragmites australis$\rightarrow$Phacelurus latifolius. Suaeda japonica has the highest dominance among the species composition and Aster tripolium, Phragmites australis, Artemisia scoparia, Carex scabrifolia and Phacelurus latifolius are distributed as zonation or patch. By the Z-M method eleven plant communities were recognized; Suaeda japonica, Suaeda japonica-Suaeda maritima, Suaeda maritima, Suaeda japonica-Aster tripolium, Aster tripolium, Phragmites australis, Carex scabrifolia, Phacelurus latifolius, Artemisia scoparia-Aster tripolium, Paspalum distichum var. indutum and Aster tripolium-Artemisia scoparia community. The actual vegetation map was constructed of the grounds of the communities classified and other data.

• Korean Journal of Environment and Ecology
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• v.28 no.1
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• pp.80-89
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• 2014

This study of the school's outdoor space for relaxing and activity of the two most numerous students, high heat and low heat with a green space, playground targets of thermal comfort indicators UTCI, PMV, WBGT using the thermal comfort students feeling compare the analyzed. The destination of this study, school facilities of Nam-gu, Namdong- gu, Incheon were studied and the investigation period was conducted from July to August. List of measurement, in the case of thermal comfort indicators, UTCI, PMV, WBGT was measured in the case of green, ratio of green coverage and GVZ was measured. GVZ analysis were as follows: A school ($4.71m^3/m^2$) B school ($3.34m^3/m^2$) C school ($0.38m^3/m^2$). Comparative analysis of the results of thermal comfort indicators by schools, UTCI was Green space $26.15{\sim}31.38^{\circ}C$ and playground $40.66{\sim}42.94^{\circ}C$, PMV values were 1.76 to 2.66 as a green space. WBGT was Green space $26.15{\sim}31.38^{\circ}C$, playground $31.67{\sim}34.53^{\circ}C$. Comparative analysis of the results of thermal comfort indicators UTCI, PMV, WBGT all A school, B school, C school, on the green space was comfortable levels more than playground. The results of the school type thermal comfort and green correlation analysis of thermal comfort UTCI, PMV, WBGT all solar radiation, globe temperature, and a positive correlation shown solar radiation, globe temperature is not comfortable, the higher was considered. UTCI, PMV, WBGT of thermal comfort indicator all ratio of green coverage, GVZ and negative correlation appears ratio of green coverage, GVZ was increased due to the lowering of the value of thermal comfort indicators was considered to be comfortable.

• Journal of Wetlands Research
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• v.17 no.1
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• pp.62-74
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• 2015

Urban areas are variously under threat including deterioration of ecological functions. Many pond wetland types have been created as part of an effort to improve and restore this urban environment. This study was arranged to examine improvement plans of wetlands in urban areas by analyzing semi-natural wetlands in farm areas. As for environment for water quality, it suggested the inflow of natural water neighboring rivers or the direct inflow of rain as the improvement plans. The result which analyzed soil pH, OM, and T-N content of the soil environment mentioned that urban areas supplied artificial sluices, removed apoptotic bodies, and used artificial soil and waterproofing materials and use of natural materials in design and construction, the sluice state of the natural form, and negligence of autumn plants were suggested as the improvement plans. Florae appeared in the subject sites of the study have found that there are 35 families 69 species in urban areas and 53 families 142 species in rural areas. As the average has found that there are 18.5 families 29.3 species in 4 urban areas and 26.3 families 53.5 species in 4 rural areas, the big difference between them was analyzed. As the cause has found that there are differences in yearly plants in farming areas when compared to urban areas, creation of various basic environments including soil and water quality was suggested to make yearly plants settle down widely. Naturalized plants have found that there are no big differences between urban areas and rural areas. However, the average of the naturalized ratio in urban areas is 17.4% as the naturalized plants are about 1/4 of the appeared plants. As it was analyzed to be higher than 7.7%, the average of the naturalized ratio in farming areas as the big difference, creation of various inhabiting environments was suggested to make more yearly plants appear like the analyzed result of the life type. Consideration of placement, materials, and inhabiting environments was suggested to make creation of wetlands well appreciated to improve functions of wetlands in urban areas. It is expected that the above results of the study will be utilized in creation and improvement of the pond wetlands which can play a huge role in increase and improvement of biological diversity in urban areas.

• Journal of Life Science
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• v.18 no.8
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• pp.1123-1131
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• 2008

This study was performed to confirm of biological function of black garlic, it heated for 11 days at $40{\sim}90^{\circ}C$ (first step: heate for 2 days at $90^{\circ}C$, second step: heated for 4 days at $80^{\circ}C$, third step: heated for 4 days at $60^{\circ}C$ and fourth step: heated for 1 day at $40^{\circ}C$). Samples were analyzed physico-chemical characteristics and antioxidant activity. Hunter L, a and b values were decreased during processing, and then inner part Hunter values were highly decreased at the second step. The moisture contents were decreased to 58.48${\pm}$0.41 g/100 g at fourth step. pH was also acidified to pH 4.22${\pm}$0.02, but O.D. value at 420 nm was increased during processing of black garlic. At fourth step, total phenolics and flavonoids contents were increased about 1.9 and 2.6 folds than first step sample. Also, total pyruvate and thiosulfinate contents were increased about 1.6 and 5.8 folds as change of total phenolic and flavonoid contents, respectively. Fructose contents were the highest level among free sugars and its contents increased to 2,454.45${\pm}$4.20 mg/100 g. Contents of sucrose and maltose were decreased during processing of black garlic. The contents of total minerals were the highest at fourth step (1,009.20${\pm}$6.91 mg/100 g) during its processing. Contents of glutamic acid, proline and aspartic acid were detected higher than other composition amino acids. Taurine and ethanolamine were not detected in the first step sample, but they were detected 0.88${\pm}0.60{\sim}1.06{\pm}$0.04 and 0.28${\pm}0.4{\sim}0.5{\pm}$0.09 mg/100 g in next processing step, respectively. DPPH radical scavenging ability of water and ethanol extracts from black garlic was increased during its processing. Abilities of DPPH radical scavenging were the highest in fourth step sample, its abilities were 67.40${\pm}$0.21% in 1,000 ${\mu}g/ml$ of water extracts. Reducing power was also significantly higher in water extract than ethanol extract on the whole.

• Journal of Biomedical Engineering Research
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• v.19 no.1
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• pp.69-80
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• 1998

This research was designed to investigate how the exercise program affects paraplegic standing and walking employing functional electrical stimulation(FES). Emphasis was also given to fatigue of major lower extremity muscles induced by different types of electrical stimulation. We applied continuous and intermittent rectangular pulse trains to quadriceps of 10 normal subjects and 4 complete paraplegic patients. The frequencies were 20Hz and 80Hz, and the knee angle was fixed at 90$^{\circ}$and 150$^{\circ}$to investigate how muscle fatigue is related to muscle length. The knee extensor torque was measured and monitored. We have been training quadriceps and gastrocnemius of a male paraplegic patient by means of electrical stimulation for the past two year. FES standing was initiated when the knee extensors became strong enough to support the body weight, and then the patient started FES walking utilizing parallel bars and a walker. We used an 8-channel constant-voltage stimulator and surface electrodes. The experimental results indicated that paralyzed muscles fatigued rapidly around the optimal length contrary to normal muscles and confirmed that low frequency and intermittent stimulation delayed fatigue. Our exercise program increased muscle force by approximately 10 folds and decreased the fatigue index to half of the initial value. In addition, the exercise enabled the patient to voluntarily lift each leg up to 10cm, which was of great help to the swing phase of FES walking. Both muscle force and resistance to fatigue were significantly enhanced right after the exercise was applied every day instead of 6 days a week. Up to date, the patient can walk for more than two and half minutes at 10m/min while controlling the on/off time of the stimulator by pushing the toggle switch attached to the walker handle.

• Journal of the Korean Society of Food Science and Nutrition
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• v.31 no.5
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• pp.924-930
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• 2002

Ixeris dentata extracts exllibited antimicrobial activity against some bacteria and fungi. Also EtOH extracts showed strong antioxidant activity and RC$_{50}$ value was 28 $\mu\textrm{g}$/mL. The inhibitory effect of Ixeris dentata on the mutagenicity in Salmonella and cytotoxicity on cancer cell were studied. Ixeris dentata extracts showed anti-mutagenic effects of 78.83 and 75.96% on B(a)P in S. typhimurium TA98 and Th100, respectively. These extracts showed 78.72% antimutagenicity on TA100 against MNNG. The Ixeris dentata extract with strong antimutagenic activities was further fractionated by hexane, ethyl acetate, butanol and water. Butanol fraction was found to be highest in antimutagenic activity against MNNG than the other fractions. Butanol fraction of Ixreis dentate revealed the highest cytotoxicity against AS49 human lung carcinoma cells in which cell growth was inhibited by 93.75% at 375 $\mu\textrm{g}$/mL. Hexane fraction of ixeris dentate exhibited 68.56% inhibition against MCF-7 human breast adenocarcinoma cells at 500 $\mu\textrm{g}$/mL. Hexane fraction of Ixeris dentata exhibited 84.91% inhibition against Hep 3B human hepatocellular carcinoma cells at 500 $\mu\textrm{g}$/mL. From these results, it is considered that Ixeris dentata has strong antimutagenic and anticancer effects in vitro. However, these extracts and fractions did not show any cytotoxic effect against 293 cells.

• KSBB Journal
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• v.14 no.5
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• pp.585-592
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• 1999

This study was aimed to enhance the Fe(II) oxidation rate using immobilized cells of Thiobacillus ferroxidans. For this purpose, a medium for the minimization of jarosite formation was developed first. Secondly, cell immobilization in celite beads was carried out. And then, repeated-batch and continuous operatons of Fe(II) oxidation by using immobilization cells were performed. In a series of flask cultures, three types of media were tested: media with a much lower salt concentration than that of the 9K medium; media which contained different nitrogen sources from that of the 9K medium, that is $(NH_4)_2HPO_4$, $NH_4Cl and HNO$_3$; media which contained $(NH_4)_2HPO_4$ as nitrogen and phosphate source, but without $K_2HPO_4$ as nitrogen and phosphate source in the 9K medium. As a result, the M16 medium which contained 3 g/L of $(NH_4)_2HPO_4$ as nitrogen and phosphate source was found to be the optimal one. It sustained good cell growth allowing no jarosite formation. In the repeated-batch operations, the rate of Fe(II) oxidation gradually increased to reach a maximum value as the batch was repeated. As a result of repeated-batch operations. a maximum Fe(II) oxidation rate was 2.33 g/L . h. In the continuous operations, the iron oxidation rate could be increased to 2.14 g/L .h at a dilution rate of 0.25 $h^{-1}$ which is greater than the maximum specific growth rate (0.12 $h^{-1}$) of the bacteria.

• Journal of Korean Society of Forest Science
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• v.105 no.4
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• pp.401-413
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• 2016

Forest resources utilization and variable disturbance history have been affected the rarity and conservation value of forest relict trees, which served as habitat for forest biodiversity, important carbon stock and cultural role include human and natural history in South Korea. This study was conducted to establish the baseline data for forest resources conservation by clarifying species diversity, spatial distribution and ecological characteristics (individual and habitat) of forest relict trees (DBH > 300 cm) based on the data getting from mountain trail, high resolution aerial photos and field professionals and field survey. As results, 54 taxa (18 family 32 genus 48 species 1 subspecies 3 variety and 2 form) as about 22% of tree species in Korea was identified in the field. 837 individuals of forest relict trees were observed and the majority of the trees was in Pinaceae, deciduous Fagaceae and Rosaceae, which families are abundant in population diversity. High elevation area was important to relict trees as mean altitudinal distribution was 1,200 m a.s.l as likely affected by human activity gradients and mid-steep slope and North aspect was important environment for the trees remain. Many individuals exhibited 'damage larger branch' (55.6%) and consequent relatively lower mean canopy coverages (below 80%). Synthetically, present diversity and abundance of relict forest trees in South Korea were the result of complex process among climate variation, local weather and biological factors and the trees of big and old were estimated to important forest biodiversity elements. In the future, clarifying the role and function of relict trees in forest ecosystem, in- and ex- situ programmes for important trees and habitat, and activities for building the background of conservation policy such as "Guideline for identifying and measurement of forest relict trees".