• Reproductive and Developmental Biology
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• v.34 no.2
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• pp.95-101
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• 2010

Decorin (DCN) is a member of small leucine-rich proteoglycans which are ubiquitous components of the extracellular matrix. It regulates many physiological processes, such as matrix formation, collagen fibrillogenesis, angiogenesis, cancer growth, and cardiovascular diseases. It has been shown that DCN is expressed in the uterus during pregnancy and modulates implantation and decidualization for the establishment and maintenance of pregnancy in mice and humans. Expression of DCN in the uterine endometrium during pregnancy has not been investigated in pigs. Thus, this study investigated expression of DCN in the uterine endometrium during the estrous cycle and pregnancy in pigs. Uterine endometrial tissues were from day (D) 12 and 15 of the estrous cycle and D12, D15, D30, D60, D90, and D114 of pregnancy. Northern blot and real-time RT-PCR analyses showed that expression of DCN mRNA was detected throughout the estrous cycle and pregnancy with the highest levels during mid pregnancy. In situ hybridization analysis showed that DCN mRNA was localized to both luminal and glandular epithelia during the estrous cycle and pregnancy and also to chorionic membrane during mid pregnancy in pigs. To determine whether endometrial expression of DCN was affected by the somatic cell nuclear transfer (SCNT) procedure, DCN mRNA levels in the uterine endometrium from gilts with SCNT embryos on D30 of pregnancy were compared with those from gilts with normal embryos using real-time RT-PCR analysis. The result showed that DCN mRNA levels in the uterine endometrium were not significantly different between gilts with normal embryos and SCNT embryos. These results suggest that DCN may play an important role for endometrial tissue remodeling during mid pregnancy, and DCN expression is not affected by the SCNT procedure at the early stage of pregnancy in pigs.

• The Journal of the Acoustical Society of Korea
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• v.24 no.4E
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• pp.132-138
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• 2005

The purpose of this study is to measure the acoustic properties of Tofu and Acorn Curd (Dotori Muk), which are possibly used as tissue mimicking materials (TMMs). Due to its availability and low cost, Tofu was suggested as a TMM by several researchers who measured only sound speed and attenuation. The acoustic properties of Tofu and Muk including the backscattering coefficient were measured in this paper. Sound speed was measured by the time shift in a pulse echo setup. Attenuation coefficients and backscattering coefficients were measured by a broadband method using both 5 MHz and 10 MHz transducers in the frequency domain. The measured acoustic properties of both Tofu and Muk are observed to be similar to those of biological tissues such as beef liver or beef heart.

• Nutrition Research and Practice
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• v.2 no.1
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• pp.17-21
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• 2008

Aloin is a physiologically active anthraquinone present in aloe. There are two isomers of aloin, aloin A and aloin B, occurring as a mixture of diastereomers. The objective of this study was to determine the bioavailability and tissue distribution of aloin. Rats were gavaged with 11.8g/kg aloin, and the levels of aloin and its conjugates were measured in plasma, tissues, and urine. Plasma aloin level showed a peak at 1hr after the administration and the concentration was $59.07{\pm}10.5\;ng/ml$. The 24 h cumulated urinary aloin was 0.03% of the initial dose. These results suggest that aloin is absorbed and reaches a peak plasma level within 1-1.5 h after the administration and a significant portion is possibly metabolized or is excreted in feces. These results can apply to the determination of the adequate intake level of aloe and aloe products to achieve the desired biological effect, and to interprete in vitro study results.

• Journal of Biomedical Engineering Research
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• v.28 no.3
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• pp.392-396
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• 2007

In this paper, we develop a new system to visualize the blood flow map in skin tissue, using the technique of Laser Speckle Flowgraphy (LSFG). The measuring unit consists of the laser diode, imaging system, line sensor, scanning mirror, and one-board microcomputer. The speckle signal is analyzed and sent to a PC, where the blood flow in a tissue area of $14mm{\times}26 mm$ is evaluated and displayed in a 2-D color map. It is demonstrated that the new LSFG instrument is useful to evaluate the degree of allergic reaction in patch test.

• Journal of Biomedical Engineering Research
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• v.7 no.2
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• pp.117-118
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• 1986

It is well known that nonlinear propagation characteristics of the wave in the tissue may give very useful information for the medical diagnoisis. In this paper, a new method to detect nonlinear propagation characteristics of the internal vibration in the tissue for the low frequency mechanical vibration by using bispectral analysis is proposed. In the method, low frequency vibration of f0( = 100Hz) is applied on the surface of the object, and the waveform of the internal vibration x (t) is measured from Doppler frequency modulation of silmultaneously transmitted probing ultrasonic waves. Then, the bispectra of the signal x (t) at the frequencies (f0, f0) and (f0, 2f0) are calculated to estimate the nonlinear propagation characteristics as their magnitude ratio, w here since bispectrum is free from the gaussian additive noise we can get the value with high S/N. Basic experimental system is constructed by using 3.0 MHz probing ultrasonic waves and the several experiments are carried out for some phantoms. Results show the superiority of the proposed method to the conventional method using power spectrum and also its usefulness for the tissue characterization.

• Nutritional Sciences
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• v.5 no.3
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• pp.129-133
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• 2002

Obesity is a major public health problem in western countries. Genetic and environmental factors, separately or in combination are major determinants of fat mass. Both central effectors (primarily hypothalamus) and peripheral tissues (such as adipose tissue) are implicated in the pathogenesis of obesity. A significant number of studies have documented potential contribution of adipose tissue -via its newly discovered secretory function- to the pathogenesis of obesity and co-morbid conditions including cardiovascular disease, diabetes and hypertension. Applications of analytical techniques such as genomics and proteomics have enabled better understanding of biological sciences in general and have only being applied recently to nutritional sciences including obesity research. Here, we review the recent progress in adipose tissue functional genomics and proteomics, and the importance of these studies in energy metabolism and obesity research.

• Journal of Biomedical Engineering Research
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• v.11 no.2
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• pp.227-232
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• 1990

As a part of the study on ultrasonic tissue characterization, conventional ultrasonic imaging system is interfaced to the personal computer to acquire raw ultrasonic signal. One approach for tissue charaterization is performed using the attenuation map to the conventional images and the resulting attenuation map images are compared and inspected inside the region of interest from the viewpoint of pattern analysis. Currently, these methods are applied and modified to effectively find out the differences between the normal control and the patients with liver cirrhosis.

• Proceedings of the KOSOMBE Conference
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• v.1993 no.11
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• pp.63-66
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• 1993

Localized NMR spectra were obtained from phantom by OSIRIS technique. The selected volume, which can be controlled by frequency and its bandwidth, was 0.125cc out of 25cc and free of contamination from outer volume. With this technique NMR spectrum of a living tissue can be obtained without biopsy. i.e. in vivo state in which the metabolism of tissue may be quite different from in in vitro state. It is expected of this technique to be useful in the study of metabolism of living tissue as well as in diagnosis of deseases.

• Medical Lasers
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• v.10 no.2
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• pp.76-81
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• 2021

Biological tissues and organs are composed of different arrays of cells, biochemical cues, and extracellular matrices arranged in a complex microarchitecture. Laser-Assisted Bioprinting (LAB) is an emerging and promising technology that is reproducible with high accuracy that can be used for fabricating complex bioengineered scaffolds that mimic tissues and organs. The LAB process allows researchers to print intricate structural scaffolds using cells and different biomaterials essential for facilitating cell-scaffold interaction and to induce tissue and organ regeneration which cannot be achieved in a traditional scaffold fabrication. This process can fabricate artificial cell niches or architecture without affecting cellular viability and material integrity. This review tackles the basic principles and key aspects of Laser-Assisted Bioprinting. Recent advances, limitations, and future perspectives are also discussed.

• Journal of Life Science
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• v.19 no.5
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• pp.561-565
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• 2009

Human Tissue factor is an essential enzyme activator that forms a catalytic complex with factor VII/ VIIa, and catalyzes both the extrinsic and intrinsic blood coagulation cascades. The extracellular domain of human tissue factor is responsible for association with the biological partner. The efficient procedures for preparing biologically active human tissue factor are essential for the preclinical and clinical studies with coaguligands. An expression vector in Escherichia coli has been constructed to direct the production of extracellular human tissue factor without a fusion protein or a $His_6$ at the N-terminus. The recombinant human tissue factor was expressed in large amounts as a non-native state in E. coli. The recombinant protein was simply renatured during the DEAE-sephacel chromatographic purification procedure. Our expression and purification system does not require a protease treatment or an additional chromatographic step to remove a fusion contaminant, which provides a very useful alternative to conventional expression systems for the production of human tissue factor.