• Biotechnology and Bioprocess Engineering:BBE
• /
• 제5권4호
• /
• pp.275-287
• /
• 2000

Fermentation strategies for recombinant protein production in Pichia pastoris have been investigated and are reviewed here. Characteristics of the expression system, such as phenotypes and carbon utilization, are summarized. Recently reported results such as growth model establishment, app58lication of a methanol sensor, optimization of substrate feeding strategy, DOstat controller design, mixed feed technology, and perfusion and continuous culture are discussed in detail.

• 한국농공학회:학술대회논문집
• /
• 한국농공학회 2005년도 학술발표논문집
• /
• pp.156-163
• /
• 2005

This study was attempted to provide some fundamental data for safety structrural design of biological production facility. Wind load and snow load, acting on agricultural structures is working more sensitive than any other load. Therefore, wind speed and snow depth according to return periods for design load estimation were calculated by frequency analysis using the weather data(maximum instantaneous wind speed, maximum wind speed, maximum depth of snow cover and fall) of 68 regions in Korea. Equations for estimating maximum instantaneous wind speed with maximum wind speed were developed for all, inland and seaside regions. The results were about the same as the current eqution in general. Design wind speed and snow depth according to return periods were calculated and Local design wind load and snow load depending on return periods were presented together with iso-wind speed and iso-snow depth maps. The calculated design snow depth by maximum depth of snow cover were higher than design snow depth by maximum depth of snow fall. Considering wind speed and snow depth, protected cultivation is very difficult in Ullungdo, Gangwon seaside and contiguity inland regions, and strong structural design is needed in the west-south seaside against wind speed, and structure design of biological production facility in these regions need special consideration.

• Asian-Australasian Journal of Animal Sciences
• /
• 제13권5호
• /
• pp.625-629
• /
• 2000

Six yearling Suffolk ewes were used to study the effect of different roughage proportion diets (30%=LR, and 70%=HR) and intake levels (0.7 M and 1.3 M) on heat production and thermoregulatory responses in sheep exposed to different ambient temperatures (20 and $30^{\circ}C$). Sheep fed HR had higher heat production (HP) and time spent eating (TSE) and lower time spent standing (TSS) than those fed LR. But effect of roughage proportion on vaginal temperature (Tv) was obvious only at high intake and at $30^{\circ}C$. Sheep fed high intake had higher Tv, HP, TSS, and TSE than those fed low intake. Roughage proportion and intake level did not have an effect on respiration rate (RR), but ambient temperature did. Ambient temperature did not have an effect on HP, TSS and TSE. At $30^{\circ}C$ sheep had higher Tv and RR than those at $20^{\circ}C$. There were interactions between intake level and ambient temperature in TSS, between intake level and roughage proportion in TSE, and between roughage proportion and ambient temperature in HP. Results indicate that high roughage diet imposes a greater potential heat load on animals than low roughage diet when given at high ambient temperature, but not at low ambient temperature. And the effects is more pronounced at high intake.

• 생물환경조절학회지
• /
• 제1권2호
• /
• pp.148-153
• /
• 1992

본 연구는 생물생산시설의 구조설계 과정에서 초기단계의 고정하중을 합리적으로 추정하기 위한 식을 유도하여 구조설계 기준 설정에 기초자료를 제공하고, 생물생산분야 종사자가 간편하게 구조적 안전성을 진단할 수 있는 방법을 개발할 목적으로 수행하였으며 연구 결과를 요약하면 다음과 같다. 1. 국내의 온실설계자료를 수집하여 실제 하중을 산출, 분석해 본 결과 기존의 일본 시설원예기준 적용치와는 차이가 있었으며, 잠정적으로 국내 시설에의 적용을 위한 추정식을 유도하였다. 2. 지역별 설계하중을 적용하여 구조해석을 실시하고, 발생되는 응력의 크기에 따라서 풍하중 및 설하중 지배지역으로 안전설계 지역구분을 실시하였다.

• 상하수도학회지
• /
• 제20권1호
• /
• pp.115-121
• /
• 2006

The purpose of this study was to investigate applicability of pretreatment on the existing biological treatment for domestic wastewater reclamation. From Jar Tests, it was found that optimum dosage of coagulant was PAC 0.5mg/L and $FeCl_3$ 180mg/L for urban sewage. In this study, PAC 0.5mg/L was selected considering sludge production and the amount of coagulant required. In a continuous experiment performed with combining chemical coagulation and biological treatment, a considerable removal efficency was obtained in term of BOD, SS, T-N, T-P and ABS. When the raw sewage was supplied into the pre-chamical treatment facility, the removal of BOD and SS was 48.3% and 81.1%. However T-N removal was very low which means T-N consists of $NH_3-N$ mostly. T-P was almost completely recluced by the chemical addition. The effluent BOD & SS was 57~76 and 21~43mg/L, which could reduce the size of biological treatment facility. From the cost estimation pre-chemical treatment could save around half of the area required for biological treatment with post ceagulation.

• 생물환경조절학회지
• /
• 제1권1호
• /
• pp.1-13
• /
• 1992

본 연구는 생물생산시설의 안전구조설계기준 마련을 위한 기초자료를 제공하기 위하여, 우리나라의 60개 지역에 대한 기상자료를 사용하여 설계하중 산정에 필요한 재현기간별 설계풍속 및 설계 적설심을 구하고 등풍속선도 및 등적설심선도를 작성하였으며, 얻어진 결과를 요약하면 다음과 같다. 1. 년최고치 계열의 최대풍속 및 최대적설심 자료에 대한 Type-I 극치분포의 적정 확률분포형 검정을 위해 Chi-Square검정을 실시한 결과 대부분 지역에서 적합성이 인정되었다. 2. 적정 확률분포형으로 설정된 Type-I 극치분포에 의하여 재현기간별 표본 수에 따른 빈도계수를 구하고, 각 지역의 설계풍속 및 설계적설심을 구하였으며 결과는 Table 5와 Table 6, 그리고 Fig.3 및 Fig.4와 같다. 3. 최대풍속의 최빈풍향 분석결과 최대풍속의 약 46.4 %가 일정한 방향인 것으로 나타났으며, 지형이나 환경상의 문제가 없을 경우 최빈풍향을 고려해 주는 시설배치로 구조적 안전성을 향상시킬 수 있을 것으로 생각된다. 4. 우리나라의 풍속 및 적설심을 고려해 볼 때 울릉도 및 영동지방은 시설재배지역으로 매우 불리하며, 충남과 전북 서해안 지역은 적설에, 서남해안 지역은 바람에 강한 구조로 설계하는 것이 바람직하다는 것을 확인할 수 있었다.

• 한국생물환경조절학회:학술대회논문집
• /
• 한국생물환경조절학회 1996년도 국제심포지움 21세기 첨단식물생산시스템의 실용화
• /
• pp.1-10
• /
• 1996

Factory-style plant production systems of various kinds are the final goal of greenhouse production systems. These systems facilitate planning for constant productivity per unit area and labor under various outside weather conditions, although energy consumption is intensive. Physical environmental control in combination with biological control can replace the use of agricultural chemicals such as insecticides, herbicides and hormones to regulate plants. In this way, closed systems which do not use such agricultural chemicals are ideal for environmental conservation for the future. Nutrient components in plants can be regulafied by physical environmental control including nutrient solution control in hydroponics. Therefore, specific contents of nutrients for particular plants can be listed on the container and be used as the basis of customer choice in the future. Plant production systems can be classified into three types based on the type of lighting: natural lighting, supplemental lighting and completely artificial lighting (Plant Factory). The amount of energy consumption increases in this order, although the degree of weather effects is in the reverse order. In the addition to lighting, factory-style plant production systems consist of mechanized and automated systems for transplanting, environmental control, hydroponics, transporting within the facility, and harvesting. Space farming and development of pharmaceutical in bio-reactors are other applications of these types of plant production systems. Various kinds of state-of-art factory-style plant production systems are discussed in the present paper. These systems are, in general, rather sophisticated and mechaized, and energy consumption is intensive. Factory-style plant production is the final goal of greenhouse production systems and the possibilities for the future are infinte but not clear.

• Nuclear Engineering and Technology
• /
• 제54권1호
• /
• pp.262-268
• /
• 2022

This paper introduces a novel concept of the pulsed neutron facility (PNF) for maximizing the production of the thermal neutrons and its application to medical use based on prompt gamma neutron activation analysis (PGNAA) using Monte Carlo simulations. The PNF consists of a compact D-T neutron generator, a graphite pile, and a detection system using Cadmium telluride (CdTe) detector arrays. The configuration of fuel pins in the graphite monolith and the design and materials for the moderating layer were studied to optimize the thermal neutron yields. Biological samples - normal and cancerous breast tissues - including chlorine, a trace element, were used to investigate the sensitivity of the characteristic γ-rays by neutron-trace material interactions and the detector responses of multiple particles. Around 90 % of neutrons emitted from a deuterium-tritium (D-T) neutron generator thermalized as they passed through the graphite stockpile. The thermal neutrons captured the chlorines in the samples, then the characteristic γ-rays with specific energy levels of 6.12, 7.80 and 8.58 MeV were emitted. Since the concentration of chlorine in the cancerous tissue is twice that in the normal tissue, the count ratio of the characteristic g-rays of the cancerous tissue over the normal tissue is approximately 2.

• 상하수도학회지
• /
• 제38권1호
• /
• pp.29-38
• /
• 2024

Wastewater management is increasingly emphasizing economic and environmental sustainability. Traditional methods in sewage treatment plants have significant implications for the environment and the economy due to power and chemical consumption, and sludge generation. To address these challenges, a study was conducted to develop the Intermittent Cycle Extended Aeration System (ICEAS). This approach was implemented as the primary technique in a full-scale wastewater treatment facility, utilizing key operational factors within the standard Sequencing Batch Reactor (SBR) process. The optimal operational approach, identified in this study, was put into practice at the research facility from January 2020 to December 2022. By implementing management strategies within the biological reactor, it was shown that maintaining and reducing chemical quantities, sludge generation, power consumption, and related costs could yield economic benefits. Moreover, adapting operations to influent characteristics and seasonal conditions allowed for efficient blower operation, reducing unnecessary electricity consumption and ensuring proper dissolved oxygen levels. Despite annual increases in influent flow rate and concentration, this study demonstrated the ability to maintain and reduce sludge production, electricity consumption, and chemical usage. Additionally, systematic responses to emergencies and abnormal situations significantly contributed to economic, technical, and environmental benefits.

• 한국균학회지
• /
• 제32권2호
• /
• pp.89-94
• /
• 2004

본 실험은 다양한 농가부산물들이 노루궁데이버섯의 재배를 위한 기질로써 적합한지를 알아보기 위하여 실시되었다. 이를 위해 참나무 톱밥, 사탕수수박, 코코낫박, 율무박, 왕겨, 배추, 면자각의 기본 기질들과 미강, 옥수수박의 첨가물을 서로 다른 비율과 조성을 통하여 노루궁뎅이버섯 균주를 접종한 후, 균사생장과 균사밀도, 자실체 생산량, 생물학적 효율을 조사 비교하였다. 참나무 톱밥 80%+미강 20%의 기질이 우수한 균사생장, 균사밀도, 자실체 생산량, 생물학적 효율을 보여 종균용 기질로써 적합하였다. 이러한 기질 조성의 적합성은 병재배법을 이용하는 농가에서 3개 노루궁뎅이버섯 균주를 사용하여 행한 실증실험에서 입증되었다. 그러나 봉지재배법을 이용한 농가 실증시험에서는 면자각 40%+참나무 톱밥 40% 기질에 옥수수 및 미강을 각각 10%씩 영양원으로 첨가한 기질에서 자실체 생산 및 생물학적 효율이 우수한 것으로 나타났다.