• Biotechnology and Bioprocess Engineering:BBE
• /
• v.5 no.4
• /
• pp.275-287
• /
• 2000

Fermentation strategies for recombinant protein production in Pichia pastoris have been investigated and are reviewed here. Characteristics of the expression system, such as phenotypes and carbon utilization, are summarized. Recently reported results such as growth model establishment, app58lication of a methanol sensor, optimization of substrate feeding strategy, DOstat controller design, mixed feed technology, and perfusion and continuous culture are discussed in detail.

• Proceedings of the Korean Society of Agricultural Engineers Conference
• /
• 2005.10a
• /
• pp.156-163
• /
• 2005

This study was attempted to provide some fundamental data for safety structrural design of biological production facility. Wind load and snow load, acting on agricultural structures is working more sensitive than any other load. Therefore, wind speed and snow depth according to return periods for design load estimation were calculated by frequency analysis using the weather data(maximum instantaneous wind speed, maximum wind speed, maximum depth of snow cover and fall) of 68 regions in Korea. Equations for estimating maximum instantaneous wind speed with maximum wind speed were developed for all, inland and seaside regions. The results were about the same as the current eqution in general. Design wind speed and snow depth according to return periods were calculated and Local design wind load and snow load depending on return periods were presented together with iso-wind speed and iso-snow depth maps. The calculated design snow depth by maximum depth of snow cover were higher than design snow depth by maximum depth of snow fall. Considering wind speed and snow depth, protected cultivation is very difficult in Ullungdo, Gangwon seaside and contiguity inland regions, and strong structural design is needed in the west-south seaside against wind speed, and structure design of biological production facility in these regions need special consideration.

• Asian-Australasian Journal of Animal Sciences
• /
• v.13 no.5
• /
• pp.625-629
• /
• 2000

Six yearling Suffolk ewes were used to study the effect of different roughage proportion diets (30%=LR, and 70%=HR) and intake levels (0.7 M and 1.3 M) on heat production and thermoregulatory responses in sheep exposed to different ambient temperatures (20 and $30^{\circ}C$). Sheep fed HR had higher heat production (HP) and time spent eating (TSE) and lower time spent standing (TSS) than those fed LR. But effect of roughage proportion on vaginal temperature (Tv) was obvious only at high intake and at $30^{\circ}C$. Sheep fed high intake had higher Tv, HP, TSS, and TSE than those fed low intake. Roughage proportion and intake level did not have an effect on respiration rate (RR), but ambient temperature did. Ambient temperature did not have an effect on HP, TSS and TSE. At $30^{\circ}C$ sheep had higher Tv and RR than those at $20^{\circ}C$. There were interactions between intake level and ambient temperature in TSS, between intake level and roughage proportion in TSE, and between roughage proportion and ambient temperature in HP. Results indicate that high roughage diet imposes a greater potential heat load on animals than low roughage diet when given at high ambient temperature, but not at low ambient temperature. And the effects is more pronounced at high intake.

• Journal of Bio-Environment Control
• /
• v.1 no.2
• /
• pp.148-153
• /
• 1992

본 연구는 생물생산시설의 구조설계 과정에서 초기단계의 고정하중을 합리적으로 추정하기 위한 식을 유도하여 구조설계 기준 설정에 기초자료를 제공하고, 생물생산분야 종사자가 간편하게 구조적 안전성을 진단할 수 있는 방법을 개발할 목적으로 수행하였으며 연구 결과를 요약하면 다음과 같다. 1. 국내의 온실설계자료를 수집하여 실제 하중을 산출, 분석해 본 결과 기존의 일본 시설원예기준 적용치와는 차이가 있었으며, 잠정적으로 국내 시설에의 적용을 위한 추정식을 유도하였다. 2. 지역별 설계하중을 적용하여 구조해석을 실시하고, 발생되는 응력의 크기에 따라서 풍하중 및 설하중 지배지역으로 안전설계 지역구분을 실시하였다.

• Journal of Korean Society of Water and Wastewater
• /
• v.20 no.1
• /
• pp.115-121
• /
• 2006

The purpose of this study was to investigate applicability of pretreatment on the existing biological treatment for domestic wastewater reclamation. From Jar Tests, it was found that optimum dosage of coagulant was PAC 0.5mg/L and $FeCl_3$ 180mg/L for urban sewage. In this study, PAC 0.5mg/L was selected considering sludge production and the amount of coagulant required. In a continuous experiment performed with combining chemical coagulation and biological treatment, a considerable removal efficency was obtained in term of BOD, SS, T-N, T-P and ABS. When the raw sewage was supplied into the pre-chamical treatment facility, the removal of BOD and SS was 48.3% and 81.1%. However T-N removal was very low which means T-N consists of $NH_3-N$ mostly. T-P was almost completely recluced by the chemical addition. The effluent BOD & SS was 57~76 and 21~43mg/L, which could reduce the size of biological treatment facility. From the cost estimation pre-chemical treatment could save around half of the area required for biological treatment with post ceagulation.

• Journal of Bio-Environment Control
• /
• v.1 no.1
• /
• pp.1-13
• /
• 1992

This study was attemped to provide some fundamental data for the safety structural design of biological production facility. Wind speed and snow depth according to recurrence intervals for design load estimation were calculated by frequency analysis using the weather data of 60 stations in Korea. The following results were obtained : 1. Type-I extremal distribution was selected for the probability density function of yearly maximum wind speed and snow depth and result of Chi-square goodness of fit showed highly significance at most regions. 2. Design frequency factors for given number of samples and recurrence intervals were calculated, and also design wind speed and snow depth as shown in Table 5-Table 6 and Fig.3-Fig.4 were derived. 3. About 46.4% of the winds having maximum wind speed at every station was analyzed to be same direction, and the consideration of this fact may improve the structural safety. 4. Considering wind speed and snow depth, protected cultivation is very difficult in Ullungdo and the Youngdong districts, and strong structural design is needed in the Chungnam and Junbuk west seaside against snow depth and the west-south seaside against wind speed in Korea.

• Proceedings of the Korean Society for Bio-Environment Control Conference
• /
• 1996.05a
• /
• pp.1-10
• /
• 1996

Factory-style plant production systems of various kinds are the final goal of greenhouse production systems. These systems facilitate planning for constant productivity per unit area and labor under various outside weather conditions, although energy consumption is intensive. Physical environmental control in combination with biological control can replace the use of agricultural chemicals such as insecticides, herbicides and hormones to regulate plants. In this way, closed systems which do not use such agricultural chemicals are ideal for environmental conservation for the future. Nutrient components in plants can be regulafied by physical environmental control including nutrient solution control in hydroponics. Therefore, specific contents of nutrients for particular plants can be listed on the container and be used as the basis of customer choice in the future. Plant production systems can be classified into three types based on the type of lighting: natural lighting, supplemental lighting and completely artificial lighting (Plant Factory). The amount of energy consumption increases in this order, although the degree of weather effects is in the reverse order. In the addition to lighting, factory-style plant production systems consist of mechanized and automated systems for transplanting, environmental control, hydroponics, transporting within the facility, and harvesting. Space farming and development of pharmaceutical in bio-reactors are other applications of these types of plant production systems. Various kinds of state-of-art factory-style plant production systems are discussed in the present paper. These systems are, in general, rather sophisticated and mechaized, and energy consumption is intensive. Factory-style plant production is the final goal of greenhouse production systems and the possibilities for the future are infinte but not clear.

• Nuclear Engineering and Technology
• /
• v.54 no.1
• /
• pp.262-268
• /
• 2022

This paper introduces a novel concept of the pulsed neutron facility (PNF) for maximizing the production of the thermal neutrons and its application to medical use based on prompt gamma neutron activation analysis (PGNAA) using Monte Carlo simulations. The PNF consists of a compact D-T neutron generator, a graphite pile, and a detection system using Cadmium telluride (CdTe) detector arrays. The configuration of fuel pins in the graphite monolith and the design and materials for the moderating layer were studied to optimize the thermal neutron yields. Biological samples - normal and cancerous breast tissues - including chlorine, a trace element, were used to investigate the sensitivity of the characteristic γ-rays by neutron-trace material interactions and the detector responses of multiple particles. Around 90 % of neutrons emitted from a deuterium-tritium (D-T) neutron generator thermalized as they passed through the graphite stockpile. The thermal neutrons captured the chlorines in the samples, then the characteristic γ-rays with specific energy levels of 6.12, 7.80 and 8.58 MeV were emitted. Since the concentration of chlorine in the cancerous tissue is twice that in the normal tissue, the count ratio of the characteristic g-rays of the cancerous tissue over the normal tissue is approximately 2.

• The Korean Journal of Mycology
• /
• v.32 no.2
• /
• pp.89-94
• /
• 2004

This study was carried out to investigate the suitability of various agricultural by-products as basal substrates for the mycelial growth and fruiting body formation of Hericium erinaceum. For this aim, oak sawdust, cotton waste, sugarcane bagasse, Job's tears, rice hull, Chinese cabbage, and coconut waste were used as sole or mixed substrate(s). Corn waste and rice bran were used as nutrient supplements. The growth and density of mycelium, yield of fruiting body, and biological efficiency were compared among tested substrates colonized by Hericium erinaceum. The best measurement of mycelial growth and density, yield of fruiting body, and biological efficiency in a laboratory test was found in a spawn substrate composed with oak sawdust 80% and rice bran 20%. The suitability of this spawn substrate composition for Hericium fruiting body production was testified through practical tests in plastic bottles (850 ml) in a mushroom farm which had bottle cultivation facility. However, test in a mushroom farm which had plastic bag cultivation facility, best production of Hericium fruiting body (520 g per one bag) was observed in a spawn substrate composed of cotton waste 40%, saw dust 40%, corn waste 10%, and rice bran 10%.

• Journal of Microbiology and Biotechnology
• /
• v.18 no.3
• /
• pp.539-544
• /
• 2008

A mutant of Arthrospira platensis PCC 9108, strain M9108, obtained by mutagenesis with UV treatment, was able to mixotrophically grow in an SOT medium containing 40 g of glucose/l. The biomass and specific growth rate of strain M9108 (4.10 g/l and 0.70/d) were 1.9-fold and 1.4-fold higher, respectively, than those of the wild type (2.21 g/l and 0.58/d) under mixotrophic culture condition. In addition, when compared with the wild type, the content of ${\gamma}$-linolenic acid (GLA) in the mutant was increased when glucose concentration was increased. Compared with the wild type, the GLA content of the mutant was 2-fold higher in autotrophic culture and about 3-fold higher in mixotrophic culture. Thus, the mutant appears to possess more efficient facility to assimilate and metabolize glucose and to produce more GLA than its wild-type strain.