• 제목/요약/키워드: biological preservation

검색결과 293건 처리시간 0.031초

The biomechanical and biological effect of supercooling on cortical bone allograft

  • MuYoung Kim ;Hun-Young Yoon
    • Journal of Veterinary Science
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    • 제24권6호
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    • pp.79.1-79.16
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    • 2023
  • Background: The need for a storage method capable of preserving the intrinsic properties of bones without using toxic substances has always been raised. Supercooling is a relatively recently introduced preservation method that meets this need. Supercooling refers to the phenomenon of liquid in which the temperature drops below its freezing point without solidifying or crystallizing. Objectives: The purpose of this study was to identify the preservation efficiency and applicability of the supercooling technique as a cortical bone allograft storage modality. Methods: The biomechanical effects of various storage methods, including deep freezing, cryopreservation, lyophilization, glycerol preservation, and supercooling, were evaluated with the three-point banding test, axial compression test, and electron microscopy. Additionally, cortical bone allografts were applied to the radial bone defect in New Zealand White rabbits to determine the biological effects. The degree of bone union was assessed with postoperative clinical signs, radiography, micro-computed tomography, and biomechanical analysis. Results: The biomechanical properties of cortical bone grafts preserved using glycerol and supercooling method were found to be comparable to those of normal bone while also significantly stronger than deep-frozen, cryopreserved, and lyophilized bone grafts. Preclinical research performed in rabbit radial defect models revealed that supercooled and glycerol-preserved bone allografts exhibited significantly better bone union than other groups. Conclusions: Considering the biomechanical and biological superiority, the supercooling technique could be one of the optimal preservation methods for cortical bone allografts. This study will form the basis for a novel application of supercooling as a bone material preservation technique.

염생식물 칠면초(Suaeda japonica)의 종자발아와 유묘생장에 종자 보관기간과 토양구성이 미치는 영향 (Effects of Seed Preservation Periods and Soil Composition on the Seed Germination and Seedling Growth of Halophyte Suaeda japonica)

  • 곽용성;김시은;오지철;박서경;최한길
    • Ocean and Polar Research
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    • 제44권4호
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    • pp.311-317
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    • 2022
  • Seed germination and seedling growth of a halophyte, Suaeda japonica were examined to measure the effects of seed preservation periods and various soil compositions in the mudflat of Saemangeum, Korea. Seed germination declined from 74.30% to 44.30% as the preservation period increased from 4 months to 12 months. After 14 days of seeding, seed germination was lowest in sandy soil (SS) at 8.67% and highest in earthworm cast+leaf mold (EC+LM) at 67.33%, showing 4~5 times higher than SS. The average lengths of seedlings were 12.20 mm in SS and 42.20 mm in EC+LM, respectively. These results suggest that seed germination and seedling growth enhanced in the soil that has more organic matter. In the experiment mixing Saemangeum soil (SMS), seed germination was from 4.00% in SMS to 88.00% in the soil of SMS+EC+LM after 14 days. Similar growth patterns were observed in the length of seedlings from 5.00 mm in SMS to 49.60 mm in SMS+EC+LM. The present results indicate that a population of Suaeda japonica could be established by stimulating seed germination and seedling growth by the addition of organic matters such as EC, LM, and EC+LM in the Saemangeum mud flat.

기능성 배접지의 보존 특성 분석 -호분지- (Conservation Properties of Chalk Added Functional Lining Papers)

  • 최경화;박지희;서진호
    • 보존과학연구
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    • 통권31호
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    • pp.79-88
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    • 2010
  • Paper cultural heritages experience chemical and physical deterioration due to various factors including preservation environments and the property of paper materials1). Thus, it is important to develop optimal preservation and restoration methods for the damaged paper cultural heritages. Currently, lining is a popular treatment for the restoration of paper cultural heritages in Korea. Since lining paper is a layer of paper directly attached to the inside of cultural heritages for protection, understanding of the preservation properties of lining paper is primarily needed in order to devise the better preservation methods. The main material of lining paper is the paper mulberry, but additives such as chalk and white clay is sometimes used to enhance the preservation properties of lining paper. To date, the properties of the functional lining paper containing these additives have been not fully understood yet. In this study, dry heating aging at $105^{\circ}C$ and biological aging by the Aspergillus versicolor and Penicillium polonicum for the lining paper, which is made from paper mulberry and the chalk, are carried out to evaluate changes in their preservation properties by these aging factors. As a result, it is found that the functional lining paper containing 25.1% of chalk can control the growth of fungi, while the paper containing 32.7% of chalk do not show any protection effect. However, the functional lining paper added by chalk is more aged than the lining paper made from paper mulberry by dry heating accelerated aging.

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A Study on the Realities and Preventive Countermeasures of Child Abuse Committed by Biological Parents by information analysis.

  • Ryu, Chae-Hyoung;Yoon, Hyun-Seok
    • 한국컴퓨터정보학회논문지
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    • 제24권5호
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    • pp.171-177
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    • 2019
  • In this paper, we analyze the current phenomenon of child abuse crimes based on the information gathered by the National Child Protection Agency. The purpose of this paper is to diagnose the seriousness of child abuse by biological parents based on the analyzed information and propose measures against it. Comprehensive and integrated measures are needed for child abuse committed by biological parents due to abuse concealment, continuity of damage, and inactive national intervention by considering punishment on attackers through national intervention, fundamental in-depth psychological counseling, therapeutic care, promoting recovery of victims, and ultimately continuous and regular management and monitoring as a long-terms measure. To do so, developing customized and individual educational programs and make them obligations can be first presented to identify child abuse in advance and build up preventive systems based on the principle of family preservation. In addition, problems should be addressed at a fundamental level by performing various and active therapeutic treatments such as psychological treatment, mental treatment, or drug treatment gradually and through phases for biological parents who commit child abuse and contributing to recovering the relationship. Furthermore, proper protection and treatment service should be provided to children victimized by abuse by extending professional children care facilities and adopting the professional family commission system as measures by separating family.

Ethyl alcohol에 고정된 고등어(Scomber japonicus) 치어의 어체수축 (Body Shrinkage of Juvenile Chub Mackerel Scomber japonicus after Preservation in Ethyl Alcohol)

  • 이정훈;김정년;김주일
    • 한국수산과학회지
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    • 제45권6호
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    • pp.730-733
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    • 2012
  • We investigated body shrinkage, defined as change in standard length and body weight of juvenile chub mackerel Scomber japonicus after preservation in 70% ethyl alcohol for 1, 8, or 12 days. Standard length (SL) was decreased by 3.6% and body weight (BW) was reduced by 27.5% after 8 day of preservation. There were no significant decreases in SL and BW after 8 or 12 days preservation. Linear regression equations for estimation of original body size from the size after preservation in 70% ethyl alcohol for 12 days were expressed as: $SL_{original}=0.96SL_{preserved}+5.17$ and $BW_{original}=1.16BW_{preserved}+0.37$. These equations will be useful for improving accuracy the estimation accuracy for various size-related biological parameters in juvenile chub mackerel juvenile.

Application of bio-preservation to enhance food safety: A review

  • Nethma Samadhi Ranathunga;Kaushalya Nadeeshani Wijayasekara;Edirisinghe Dewage Nalaka Sandun Abeyrathne
    • 한국식품저장유통학회지
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    • 제30권2호
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    • pp.179-189
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    • 2023
  • Consumers and industry experts frequently have negative perceptions of most chemical preservatives. Although most people concede that they cannot resolve global food waste issues without preservatives, they prefer products without chemical preservatives. Numerous emerging technologies is now surpassing conventional methods for mitigating microbial food deterioration in response to consumer demand and fundamental health and safety considerations, including biological antimicrobial systems such as using food-grade microorganisms and their metabolites primarily originating from microorganisms, plants, and animals. Microbial compounds, including bacteriocins, bacteriophages, and anti-fungal agents, plant extracts such as flavonoids and essential oils; and animal-originated compounds, such as lysozyme, chitosan, and lactoferrin, are considered some of the major bio-preservatives. These natural compounds can be used alone or with other preservatives to improve food safety. Hence, the use of microbes or their metabolic byproducts to extend the shelf life of foods while maintaining safety standards is known as bio-preservation. To manufacture and consume foods in a safe condition, this review primarily aims to broaden knowledge amongst industry professionals and consumers regarding bio-preservation techniques, bio-preservatives, their classifications, and distinctive mechanisms to enhance food safety.

양파 페놀성분획물의 생리활성기능탐색 (Screening of Biological Activity for Phenolic Fraction from Onion)

  • 안봉전;이진태
    • 한국식품저장유통학회지
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    • 제8권2호
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    • pp.224-230
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    • 2001
  • To develop a material for functional food, the fraction Ⅰ,II and Ⅲ of onion extract were separated by Sephadex LH-20 column. The revealed purification levels of each fraction were 5.2%-6.5%, 12.2%-14.5% and 82.6%-86.7%, respectively. A functional activity test was performed with fraction Ⅲ. The ACE inhibition activity was about 50% at 5mg and about 80% at 10mg. The XOase inhibition activity of Korean Changnyong Daego was 84%, which was the highest. At the experiment of tyrosinase inhibition was detected 21-36% at 10mg. The cholesterol dose in mice blood was as low as low as 79$\pm$5(mg/100 mL) after the intake of fraction Ⅲ. Cholestrol dose in liver was decreased to below 50%. The results show that Sephadex LH-20 column method was very useful as a fraction method for the development of functional food material nsing onions.

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참외 포장용 골판지상자의 재질구성에 관한 연구 (Studies on Linerboard Compositions of Corrugated Fiberboard Boxes for Oriental Melons)

  • 하영선;이준호;김수일;박남호
    • 한국식품저장유통학회지
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    • 제7권2호
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    • pp.160-165
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    • 2000
  • The study aimed to review linerboard compositions of corrugated fiberboard boxes for Oriental melons(Cucumis melon L. var makuwa), analyzing relationships among material compositions, price, weight of boxes, theoretical compression and bursting strength of various liner boards. 19 and 22 different liner boards were currently used for the bleached and the unbleached corrugated boards, respectively. The corrugated board containers with the bleached liners were mostly used in the market although the average price was 10 percents higher than the unbleached. The average compression strength of the bleached. We strongly recommend to redesign the boxes because the average compression and bursting strength of the current boxes were much higher than national packaging standards as much as 70 and 82, respectively.

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목재문화재의 열화에 대한 고찰 (Degradation and Preservation of wood)

  • 김익주
    • 보존과학연구
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    • 통권7호
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    • pp.265-277
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    • 1986
  • The degradation of wood is maimly caused by biological and thermal factor. In general, the field of wood preservation can be divided into two broadcategories; namely the deterioration, protection of wood, and the teatment of wood with preservatives. Wood in sea or brackish water incurs marine borer damage, consisting of attack by marine animal and also wood on land suffers severely from insect damage. But the largest wood degradation is caused by microorganism. Animals that attack wood in a marine environment are especially destructive in warm water-regions, little was achieved in their control recently. Therefore this manuscript only introduce the importance of wood deterioration caused by marine animal.

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