• Title/Summary/Keyword: biological laboratory

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Comparison of immunoadjuvant activities of four bursal peptides combined with H9N2 avian influenza virus vaccine

  • Zhang, Cong;Zhou, Jiangfei;Liu, Zhixin;Liu, Yongqing;Cai, Kairui;Shen, Tengfei;Liao, Chengshui;Wang, Chen
    • Journal of Veterinary Science
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    • v.19 no.6
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    • pp.817-826
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    • 2018

  • The bursa of Fabricius (BF) is a central humoral immune organ unique to birds. Four bursal peptides (BP-I, BP-II, BP-III, and BP-IV) have been isolated and identified from the BF. In this study, the immunoadjuvant activities of BPs I to IV were examined in mice immunized with H9N2 avian influenza virus (AIV) vaccine. The results suggested that BP-I effectively enhanced cell-mediated immune responses, increased the secretion of Th1 (interferon gamma)- and Th2 (interleukin-4)-type cytokines, and induced an improved cytotoxic T-lymphocyte (CTL) response to the H9N2 virus. BP-II mainly elevated specific antibody production, especially neutralizing antibodies, and increased Th1- and Th2-type cytokine secretion. BP-III had no significant effect on antibody production or cell-mediated immune responses compared to those in the control group. A strong immune response at both the humoral and cellular levels was induced by BP-IV. Furthermore, a virus challenge experiment followed by H&E staining revealed that BP-I and BP-II promoted removal of the virus and conferred protection in mouse lungs. BP-IV significantly reduced viral titers and histopathological changes and contributed to protection against H9N2 AIV challenge in mouse lungs. This study further elucidated the immunoadjuvant activities of BPs I to IV, providing a novel insight into immunoadjuvants for use in vaccine design.

  • https://doi.org/10.4142/jvs.2018.19.6.817 인용 KSCI

New Insights into mTOR Signal Pathways in Ovarian-Related Diseases: Polycystic Ovary Syndrome and Ovarian Cancer

  • Liu, Ai Ling;Liao, Hong Qing;Li, Zhi Liang;Liu, Jun;Zhou, Cui Lan;Guo, Zi Fen;Xie, Hong Yan;Peng, Cui Ying
    • Asian Pacific Journal of Cancer Prevention
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    • v.17 no.12
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    • pp.5087-5094
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    • 2016

  • mTOR, the mammalian target of rapamycin, is a conserved serine/threonine kinase which belongs to the phosphatidyl-linositol kinase-related kinase (PIKK) family. It has two complexes called mTORC1 and mTORC2. It is well established that mTOR plays important roles in cell growth, proliferation and differentiation. Over-activation of the mTOR pathway is considered to have a relationship with the development of many types of diseases, including polycystic ovary syndrome (PCOS) and ovarian cancer (OC). mTOR pathway inhibitors, such as rapamycin and its derivatives, can directly or indirectly treat or relieve the symptoms of patients suffering from PCOS or OC. Moreover, mTOR inhibitors in combination with other chemical-molecular agents may have extraordinary efficacy. This paper will discuss links between mTOR signaling and PCOS and OC, and explore the mechanisms of mTOR inhibitors in treating these two diseases, with conclusions regarding the most effective therapeutic approaches.

  • https://doi.org/10.22034/APJCP.2016.17.12.5087 인용 KSCI