• Archives of Pharmacal Research
• /
• v.27 no.6
• /
• pp.676-681
• /
• 2004

Acetyl-L-camitine (ALC), a naturally occurring endogenous compound, has been shown to improve the cognitive performance of patients with senile dementia Alzheimer＇s type, and to be involved in cholinergic neurotransmission. Because ALC is an endogenous compound, valida-tion of the analytical methods of ALC in the biological fluids is very important and difficult. This study was presented validation and correction for plasma ALC concentrations and pharmacok-inetics after oral administration of ALC to human volunteers. ALC concentrations in human plasma were corrected by subtracting the concentration of blank plasma from each sample. Precision and accuracy (bias %) for uncorrected ALC concentrations were below 2.6 and 6.5% for intra-days, and 4.0 and 9.4% for inter-days, respectively. Precision and accuracy (bias %)for corrected ALC concentrations were below 10.9 and 6.0% for intra-days, and 10.5 and 16.9% for inter-days, respectively. Quantitation limit was $0.1{\;}\mu\textrm{g}／mL$. After oral administration of a 500 mg ALC tablet to 8 healthy volunteers, the principle pharmacokinetic parameters were 4.2 h of the half-life$ (t_{1/2},{\beta})$, the area under the curve $(AUC_{0{\rightarrow}8){\;}of{\;}9.88{\;}\mu\textrm{g}{\cdot}h/mL$, and 3.1 h of the time ($T_{max}$) to reach $C_{max}$. This study first describes the pharmacokinetic study after oral admin-istration of a single dose of ALC in human volunteers.

• Korean Journal of Plant Taxonomy
• /
• v.42 no.2
• /
• pp.178-183
• /
• 2012

We report a newly naturalized species of the genus Bidens L. (Asteraceae) in Korea, specifically Bidens subalternans DC. var. subalternans. This naturalized plant was discovered Geumjung-gu in Busan; Gyeongsan-si, Jillyang-eup in Gyeongbuk-do; and in Buk-gu in Daegu. Bidens subalternans var. subalternans is distinguishable from other Bidens in Korea by the following combination of characteristics: an odd-pinnate leaf, deeply lobed, narrow lanceolate, four lobes in the corolla, an oblong achene shape. This taxon is termed 'Wang-do-kka-bi-ba-neul' as a common Korean name based on its large size (in particular, the size of leaf). The key for all taxa of the genus Bidens in Korea is also provided here.

• The Korean Journal of Ecology
• /
• v.18 no.1
• /
• pp.109-120
• /
• 1995

As the genetically modified microorganisms (GMMs) and their recombinant plasmid DNAs could be released into natural environments, their stabilities and impacts to indigenous microorganisls have become very importhant research subjects concerning with environmental and ecological aspects. In this study, the genetically modified E. coli CU103 and its recombinant pCU103 plasmid DNA, in which pcbCD genes involving in degradation of biphenyl and 4-chlorobiphenyl were cloned, were studied for their survival and stability in several different waters established under laboratory conditions. E. coli CU103 and its host E. coli XL1-Blue survived longer in sterile distilled water (SDW) and filtered autoclaved river water (FAW) than in filtered river water (FW). A lot of extracellular DNAs were released from E. coli CU103 by lytic action of phages in FW and the released DNAs were degraded by DNase dissolved in the water. Such effects of the factors in FW on stability of the recombinant pCU103 plasmid were also observed in the results of gel electrophoresis, quantitative analysis with bisbenzimide, and transformation assay. Therefore, the recombinant plasmids of pCU103 were found to be readily liberated from the genetically modified E. coli CU103 into waters by normal metabolic processes and lysis of cells. And the plasmid DNAs were quite stable in waters, but their stabilities could be affected by physicoKDICical and biological factors in non-sterile natural waters.

• Journal of Environmental Science International
• /
• v.14 no.7
• /
• pp.675-681
• /
• 2005

A laboratory experiment was performed to investigate phosphorus and nitrogen removal from synthetic wastewater by intermittently aerated activated sludge process packed with aluminum and silver plate. Three continuous experi­mental processes, i. e. an intermittently aerated activated sludge process(Run A), an intermittently aerated activated sludge process with an aluminum and silver plate packed into the reactor(Run B), and a reactor post stage(Run C) were compared. In the batch experiments, the phosphorus removal time in the reactor packed with aluminum and silver plate simultaneously was faster than that of the reactor packed with only an aluminum plate. More phos­phorus was removed with an increase of NaCl concentration. The pitting corrosion of aluminum does not affect the performance of the biological treatment. The total nitrogen removal efficiency in Run B was $57\%\;and\;43.6\%$ at the HRT of 12 and 6 hours respectively. The effluent $PO_{4-}P$ concentration as low as 1.0 mg/L could be ob­tainable through the continuous experiment in Run B at HRT of 6 hours.

• Journal of Dental Rehabilitation and Applied Science
• /
• v.32 no.3
• /
• pp.240-245
• /
• 2016

Removal of excess cement is important to prevent biological complication in cementation of implant restoration with subgingival margin. It can be difficult to completely remove excess cement. Several techniques have been introduced to minimize excess cement using abutment replica. In this case report, a simple method for making abutment replica with hot melt adhesive material in dental office was described. This technique is simple and effective because it can be used for pre-fabricated or custom abutment without additional laboratory procedure. In addition, it can minimize excess cement after cementation of implant restoration.

• Korean journal of applied entomology
• /
• v.30 no.1
• /
• pp.50-53
• /
• 1991

The entomopathogenic nematodes, Steinernema carpocapsae and Heterorhabditis bacteriophoTa, were laboratory tested for the control of rice stem borer Chilo suppressalis. When 8 cm long of two rice stems infected by rice stem borers were treated with S. carpocapsae by spray application to the concentrations of 250, 500, 1,000, or 2,000 nematodes/ml, 68.6 $\pm$ 10.0-94.9 $\pm$ 2.6 % control was obtained. When 8 cm long of rice stems infected by rice stem borers were treated with H. bacteriophoTa by spray application to the concentrations of 100, 200, 400, or 800 nematodes/ ml, 91.4 $\pm$ 0.7-100 % control was obtained. On the other hand, when 8 cm long of three rice stems were treated with H. bacteriophora by dipping application to the concentrations of 100 or 200 nematodes/ml, 46.2 $\pm$ 4.7-63.1 $\pm$ 4.7 % control was obtained. Because the moist habitat of rice stems were favorable to nematode survival and searching abilities, entomopathogenic nematode, were confirmed to be a potential biological control agents against rice stem borers.

• Biomedical Science Letters
• /
• v.15 no.1
• /
• pp.37-45
• /
• 2009

Insect cell cultures have become important tools in the production of biological substances for use in a variety of research, human and veterinary medicine, and pest control applications. These applications often require the introduction of foreign DNA into the cells and have generally used methods originally developed for use with human and other mammalian cell cultures. While these methods can be successfully employed, they are often less efficient with insect cells and frequently involve complex procedures or require specialized equipment. Even when they do work, they may require substantial modification because of differences in the culture medium or growth patterns of insect cells. In this study, We have optimized transfection conditions of Sf9 cell line using insect expression vector pIZT/V5-His which expresses green fluorescent protein effectively. Human stem cell factor (hSCF) is a glycoprotein that plays a key role in hematopoiesis acting both as a positive and negative regulator, often in synergy with other cytokines. It also plays a key role in mast cell development, gametogenesis, and melanogenesis. It can exist in membrane-bound form and in proteolytically released soluble form. As determined by an enzyme-linked immunosorbent assay performed, hSCF level in supernatant averaged 995ng/ml. The human hSCF was partially purified by immunoaffinity chromatography and analyzed with sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting. The results show that the hSCF has N-linked carbohydrate and corresponds to the soluble form, at or about 223 amino acids in length. The findings suggest functional importance for soluble hSCF in cells.

• The Journal of Advanced Prosthodontics
• /
• v.7 no.2
• /
• pp.151-159
• /
• 2015

PURPOSE. To describe and characterize the surface topography and cleanliness of CAD/CAM manufactured zirconia abutments after steaming and ultrasonic cleaning. MATERIALS AND METHODS. A total of 12 ceramic CAD/CAM implant abutments of various manufacturers were produced and randomly divided into two groups of six samples each (control and test group). Four two-piece hybrid abutments and two one-piece abutments made of zirconium-dioxide were assessed per each group. In the control group, cleaning by steam was performed. The test group underwent an ultrasonic cleaning procedure with acetone, ethyl alcohol and antibacterial solution. Groups were subjected to scanning electron microscope (SEM) analysis and Energy-dispersive X-ray spectroscopy (EDX) to verify and characterize contaminant chemical characterization non- quantitatively. RESULTS. All zirconia CAD/CAM abutments in the present study displayed production-induced wear particles, debris as well as organic and inorganic contaminants. The abutments of the test group showed reduction of surface contamination after undergoing an ultrasonic cleaning procedure. However, an absolute removal of pollutants could not be achieved. CONCLUSION. The presence of debris on the transmucosal surface of CAD/CAM zirconia abutments of various manufacturers was confirmed. Within the limits of the study design, the results suggest that a defined ultrasonic cleaning process can be advantageously employed to reduce such debris, thus, supposedly enhancing soft tissue healing. Although the adverse long-term influence of abutment contamination on the biological stability of peri-implant tissues has been evidenced, a standardized and validated polishing and cleaning protocol still has to be implemented.

• Asian Journal of Turfgrass Science
• /
• v.18 no.4
• /
• pp.193-200
• /
• 2004

Environmentally sound control of fall webworm, Hyphanria cunea (Drury) with entomopathogenic nematode, Sreinernema carpocapsae Pocheon strain was evaluated in the laboratory and pot. Pathogenicity of 5. carpocapsae Pocheon strain was different depending on larval stage, i.e., mortality of the 2nd instar and the $3\~4th$ instar was $100\%$ with >20 infective juveniles (Ijs)/larva in 3 days, but the 5th instar was $34\%$ with (Ijs)/larva in 3 days. Pathogenicity of 5. carpocapsae Pocheon strain was higher with increasing nematode concentration. Mortality of Hyphanria cunea larva by 5. carpocapsae Pocheon strain was not significantly different (more than $70\%$) between nematode concentration on treated trees (Malus alba and Platanus orientalis) and in pot.

• Journal of Ginseng Research
• /
• v.35 no.2
• /
• pp.243-249
• /
• 2011

Korean red ginseng (KRG), the steamed root of Panax ginseng Meyer, has a variety of biological properties, including anti-inflammatory, antioxidant and anticancer effects. Aflatoxin $B_1$ ($AFB_1$) produced by the Aspergillus spp. causes acute hepatotoxicity by lipid peroxidation and oxidative DNA damage, and induces liver carcinoma in humans and laboratory animals. This study was performed to examine the protective effects of KRG against hepatotoxicity induced by $AFB_1$ using liver-specific serum marker analysis, histopathology, and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling. In addition, to elucidate the possible mechanism of hepatoprotective effects, superoxide dismutase, catalase, glutathione peroxidase, and malondialdehyde were analyzed. Rats were treated with 250 mg/kg of KRG (KRG group) or saline ($AFB_1$ group) for 4 weeks and then received 150 ${\mu}g/kg$ of $AFB_1$ intraperitoneally for 3 days. Rats were sacrificed at 12 h, 24 h, 48 h, 72 h, or 1 wk after $AFB_1$ treatment. In the KRG pre-treatment group, serum alanine aminotransferase, aspartate aminotransferase, and malondialdehyde levels were low, but superoxide dismutase, catalase, and glutathione peroxidase activities were high as compared to the $AFB_1$ alone group. Histopathologically, $AFB_1$ treatment induced necrosis and apoptosis in hepatocytes, and led to inflammatory cells infiltration in the liver. KRG pre-treatment ameliorated these changes. These results indicate that KRG may have protective effects against hepatotoxicity induced by $AFB_1$ that involve the antioxidant properties of KRG.